当前位置： SCI文献检索 > NEUROCHEMISTRY INTERNATIONAL期刊下所有文献 > Existence of high and low affinity dinucleotides pentaphosphate-induced calcium responses in individual synaptic terminals and lack of correlation with the distribution of P2X1-7 subunits.

Existence of high and low affinity dinucleotides pentaphosphate-induced calcium responses in individual synaptic terminals and lack of correlation with the distribution of P2X1-7 subunits.

Abstract：

:Individual analysis of synaptic terminals calcium responses, induced by dinucleotides pentaphosphate, Ap(5)A or Gp(5)G, demonstrates the presence of two main groups considering the concentration required for stimulation. The first group corresponds to those responding to Ap(5)A or Gp(5)G at nanomolar concentration, representing 16% and 12%, respectively, and the second one responds to micromolar concentration and represents, respectively, 17% and 14%, of the total functional synaptosomal population in rat midbrain. Dose-response curves in single terminals showed an Ap(5)A EC(50) values of 0.9+/-0.2 nM and 11.8+/-0.9 microM, being the maximal intrasynaptosomal calcium increase of 200+/-0.3 and 125+/-0.2 nM for the high and low affinity responding terminals, respectively. Combination of microfluorimetric and immunocytochemical studies showed lack of correlation between dinucleotides pentaphosphate responses and P2X receptor subunits expression, in spite of the abundance of P2X(2), P2X(3) and P2X(7) at the presynaptic level in rat midbrain synaptosomes. Pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (PPADS), a P2X receptors antagonist, showed no effect on low affinity dinucleotides receptors population, and partial inhibition on the high affinity one. On the other hand, diinosine pentaphosphate (Ip(5)I) completely abolished the low affinity dinucleotides responses, and 60% inhibition of the high affinity ones.

journal_name

Neurochem Int

journal_title

Neurochemistry international

authors

Gómez-Villafuertes R,Sánchez-Nogueiro J,Marín-García P,Miras-Portugal MT

doi

10.1016/j.neuint.2006.12.007

subject

Has Abstract

pub_date

2007-03-01 00:00:00

pages

628-41

issue

4

eissn

0197-0186

issn

1872-9754

pii

S0197-0186(06)00358-5

journal_volume

50

pub_type

杂志文章

相关文献

NEUROCHEMISTRY INTERNATIONAL文献大全
  • P53 knockout mice are protected from cocaine-induced kindling behaviors via inhibiting mitochondrial oxidative burdens, mitochondrial dysfunction, and proapoptotic changes.

    abstract：:Previously we demonstrated that p53 mediates dopaminergic neurotoxicity via inducing mitochondrial burdens and proapoptotsis. However, little is known about the role of p53 in the excitotoxicity induced by psychostimulant, such as cocaine. Cocaine-induced kindling (convulsive) behaviors significantly increased p53 exp...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2018.12.017

    authors： Mai HN,Sharma N,Jeong JH,Shin EJ,Pham DT,Trinh QD,Lee YJ,Jang CG,Nah SY,Bing G,Kim HC

    更新日期：2019-03-01 00:00:00

  • Differential modulation by muscimol of [(3)H] flunitrazepam binding to benzodiazepine binding site subtypes.

    abstract：:The effects of the GABA agonist, muscimol on [(3)H]flunitrazepam binding were examined in cerebellum and hippocampus regions proposed to contain different populations of benzodiazepine binding site subtypes. Quantitative analysis was made of the contribution of different components of [(3)H]flunitrazepam binding by ut...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(84)90082-2

    authors： Mitchell R,Wilson L

    更新日期：1984-01-01 00:00:00

  • Identification of a histaminergic circuit in the caudal hypothalamus: an evidence for functional heterogeneity of histaminergic neurons.

    abstract：:It is well established that histaminergic neurons in the posterior hypothalamus make connections with whole brain areas and regulate several functions. Recent evidence indicates that histaminergic neurons are heterogeneous cell group and organized into distinct circuits. However, functional circuits of histaminergic n...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2012.05.022

    authors： Umehara H,Mizuguchi H,Fukui H

    更新日期：2012-11-01 00:00:00

  • Regulation of intracellular [Ca2+] and GABA release by presynaptic GABAB receptors in rat cerebrocortical synaptosomes.

    abstract：:In this study we determined the changes in the intracellular free Ca2+ concentration, associated with the inhibitory modulation of the exocytotic release of GABA by GABAB receptor activation in rat cerebrocortical synaptosomes. We observed that SK&F 97541 and (-)baclofen both act as agonists of the presynaptic GABAB r...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(95)00021-y

    authors： Santos AE,Carvalho CM,Macedo TA,Carvalho AP

    更新日期：1995-10-01 00:00:00

  • 5-Hydroxytryptamine2B receptors stimulate Ca2+ increases in cultured astrocytes from three different brain regions.

    abstract：:The expression of 5-hydroxytryptamine-2B (5-HT2B) receptor mRNA has recently been shown in cultured astrocytes. Here the expression of functional 5-HT2B receptors has been studied in cultured astrocytes from rat cerebral cortex, hippocampus, and brain stem. Fluo-3- and fura-2-based microspectrofluorometry was used for...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(99)00134-5

    authors： Sandén N,Thorlin T,Blomstrand F,Persson PA,Hansson E

    更新日期：2000-04-01 00:00:00

  • Glutamine transport at the blood-brain and blood-cerebrospinal fluid barriers.

    abstract：:Glutamine has multiple physiological and pathophysiological roles in the brain. Because of their position at the interface between blood and brain, the cerebral capillaries and the choroid plexuses that form the blood-brain barriers (BBB) and blood-cerebrospinal fluid (CSF) barriers, have the potential to influence br...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(03)00013-5

    authors： Xiang J,Ennis SR,Abdelkarim GE,Fujisawa M,Kawai N,Keep RF

    更新日期：2003-09-01 00:00:00

  • Preferential release of newly synthesized [(3)H]GABA from striatal slices to preloaded [(3)H]GABA.

    abstract：:The release of [(3)H]GABA which is newly synthesized from [(3)H]l-glutamic acid (GLU) has been examined using striatal slices obtained from the rat brain. It was found that 8-10% of [(3)H]GLU transported was converted to [(3)H]GABA during the incubation of striatal slices in the presence of nipecotic acid (5 x 10(?5) ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(84)90045-7

    authors： Yoneda Y,Kanmori K,Takahashi M,Zuo PP,Paik IH,Kuriyama K

    更新日期：1984-01-01 00:00:00

  • Chlorimipramine, fenfluramine and quipazine decrease 5-hydroxytryptamine synthesis in discrete rat brain nuclei.

    abstract：:A procedure for studying 5-hydroxytryptamine synthesis by determining the rate of accumulation of 5-hydroxytryptophan after administering m-hydroxybenzylhydrazine, an inhibitor of aromatic-l-amino acid decarboxylase, and large doses of l-tryptophan was characterized. The utility of this method as an index of 5-hydroxy...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(86)90014-8

    authors： Nielsen JA

    更新日期：1986-01-01 00:00:00

  • The role of TNF-alpha/NF-kappa B pathway on the up-regulation of voltage-gated sodium channel Nav1.7 in DRG neurons of rats with diabetic neuropathy.

    abstract：:Diabetic neuropathy (DN) is a common form of peripheral neuropathy, yet the mechanisms responsible for chronic pain in this disease are poorly understood. The up-regulation of the expression and function of voltage-gated sodium channel Nav1.7 has been implicated in DN, however, the exact mechanism is unclear. In the p...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2014.05.012

    authors： Huang Y,Zang Y,Zhou L,Gui W,Liu X,Zhong Y

    更新日期：2014-09-01 00:00:00

  • Alpha-synuclein and its neurotoxic fragment inhibit dopamine uptake into rat striatal synaptosomes. Relationship to nitric oxide.

    abstract：:Alpha-synuclein (ASN), a 140-amino acid protein, is richly expressed in presynaptic terminals in the central nervous system, where it plays a role in synaptic vesicle function. However, if it is altered and accumulated it is involved in neurodegeneration as Parkinson's disease (PD). ASN contained 35-amino acid domain ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2006.01.025

    authors： Adamczyk A,Kaźmierczak A,Strosznajder JB

    更新日期：2006-09-01 00:00:00

  • α-Lipoic acid (LA) enantiomers protect SH-SY5Y cells against glutathione depletion.

    abstract：:Growing evidence suggests that α-lipoic acid (LA) has neuroprotective effects in various pathological conditions including brain ischemia and neurodegeneration. While anti-oxidative activity has been thought to play a central role in LA-mediated neuroprotection, the precise mechanism and the effect of LA enantiomers (...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2011.09.005

    authors： Yamada T,Hashida K,Takarada-Iemata M,Matsugo S,Hori O

    更新日期：2011-12-01 00:00:00

  • 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) effects on enkephalinergic neurons in various regions of mouse brain.

    abstract：:In order to elucidate the effects of MPTP on enkephalinergic neurons, dopamine (DA), norepinephrine (NE), proenkephalin (PE) mRNA and met-enkephalin (ME) were measured in striatum, olfactory tubercle, and prefrontal cortex of C57/B16 mice 1 day-2 weeks following treatment with 96 mg/kg MPTP HCl (24 mg/kg i.p., twice/d...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(93)90010-3

    authors： Mitsuo K,Cosi C,Harvey-White JD,Schwartz JP

    更新日期：1993-02-01 00:00:00

  • High-affinity choline uptake and acetylcholine-metabolizing enzymes in CNS white matter. A quantitative study.

    abstract：:The presence of nicotinic and muscarinic receptors suggests the occurrence of cholinergic neurotransmission in white matter; however no quantitative information exists on acetylcholine formation and breakdown in white matter. We compared white structures of pig brain (fimbria, corpus callosum, pyramidal tracts, and oc...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2008.06.008

    authors： Hassel B,Solyga V,Lossius A

    更新日期：2008-12-01 00:00:00

  • Long-term changes in noradrenaline (NA) and dopamine (DA) contents of rat cerebellum following neonatal X-irradiation.

    abstract：:The effects of neonatal X-irradiation on cerebellar cathecholamine levels in rats were studied at different postnatal intervals. Since synaptogenesis in the cerebellar cortex is basically a postnatal phenomenon, changes in noradrenaline (NA) and dopamine (DA) levels induced by X-rays on the cerebella (CE) of adult rat...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(89)90081-8

    authors： Dopico AM,Zieher LM,Mayo J,Altschüller N,López JJ,Jaim-Etcheverry G

    更新日期：1989-01-01 00:00:00

  • Transient coupling of NMDA receptor with ip3 production in cultured cells of the avian retina.

    abstract：:The mobilization of inositol triphosphate ip3 by N-methyl D-aspartate (NMDA) and kainate, two excitatory amino acid EAA receptor agonists, was studied in cultured chick retina cells as a function of culture differentiation. Kainate (EC50 = 30 microM) stimulated from 6 to 9-fold the production of [3H]ip3 between E8C3 (...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(94)00143-i

    authors： Reis RA,Kubrusly RC,de Mello MC,de Mello FG

    更新日期：1995-04-01 00:00:00

  • Dopamine transporters are involved in the onset of hypoxia-induced dopamine efflux in striatum as revealed by in vivo microdialysis.

    abstract：:Although many studies have revealed alterations in neurotransmission during ischaemia, few works have been devoted to the neurochemical effects of mild hypoxia, a situation encountered during life in altitude or in several pathologies. In that context, the present work was undertaken to determine the in vivo mechanism...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2005.02.005

    authors： Orset C,Parrot S,Sauvinet V,Cottet-Emard JM,Bérod A,Pequignot JM,Denoroy L

    更新日期：2005-06-01 00:00:00

  • Effect of diet on serotonergic neurotransmission in depression.

    abstract：:Depression is characterized by sadness, purposelessness, irritability, and impaired body functions. Depression causes severe symptoms for several weeks, and dysthymia, which may cause chronic, low-grade symptoms. Treatment of depression involves psychotherapy, medications, or phototherapy. Clinical and experimental ev...

    journal_title：Neurochemistry international

    pub_type： 杂志文章,评审

    doi：10.1016/j.neuint.2012.12.014

    authors： Shabbir F,Patel A,Mattison C,Bose S,Krishnamohan R,Sweeney E,Sandhu S,Nel W,Rais A,Sandhu R,Ngu N,Sharma S

    更新日期：2013-02-01 00:00:00

  • Ubiquitin dimers control the hydrolase activity of UCH-L3.

    abstract：:Ubiquitin (Ub) carboxy terminal hydrolase (UCH)-L1 and UCH-L3 are two of the deubiquitinating enzymes expressed in the brain. Both gad mice, which lack UCH-L1 expression and Uchl3 knockout mice exhibit neurodegeneration, although at distinct areas. These phenotypes indicate the importance of UCH-L1 and UCH-L3 in the r...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2008.12.013

    authors： Setsuie R,Sakurai M,Sakaguchi Y,Wada K

    更新日期：2009-05-01 00:00:00

  • Antidepressant-like effects of 3,6'-disinapoyl sucrose on hippocampal neuronal plasticity and neurotrophic signal pathway in chronically mild stressed rats.

    abstract：:Recent studies suggest that the behavioral effects of chronic antidepressant treatment are mediated by stimulation of hippocampal neuronal plasticity and neurogenesis. The present study was designed to examine the effects of 3,6'-disinapoyl sucrose (DISS), a bioactive component of Polygala tenuifolia Willd, on the exp...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2009.12.004

    authors： Hu Y,Liao HB,Dai-Hong G,Liu P,Wang YY,Rahman K

    更新日期：2010-02-01 00:00:00

  • Peroxisome proliferator-activated receptor (PPAR)β/δ, a possible nexus of PPARα- and PPARγ-dependent molecular pathways in neurodegenerative diseases: Review and novel hypotheses.

    abstract：:Peroxisome proliferator-activated receptors (PPARα, -β/δ and -γ) are lipid-activated transcription factors. Synthetic PPARα and PPARγ ligands have neuroprotective properties. Recently, PPARβ/δ activation emerged as the focus of a novel approach for the treatment of a wide range of neurodegenerative diseases. To fill t...

    journal_title：Neurochemistry international

    pub_type： 杂志文章,评审

    doi：10.1016/j.neuint.2013.06.012

    authors： Aleshin S,Strokin M,Sergeeva M,Reiser G

    更新日期：2013-10-01 00:00:00

  • Sildenafil treatment of vascular dementia in aged rats.

    abstract：BACKGROUND:and purpose: In this study, we employed a multiple microinfarction (MMI) based vascular dementia (VaD) model in aged rats and tested the therapeutic effects of Sildenafil, a phosphodiesterase type 5 inhibitor, on cognitive decline, white matter damage, autophagy and inflammatory response associated with VaD....

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2018.12.015

    authors： Venkat P,Chopp M,Zacharek A,Cui C,Landschoot-Ward J,Qian Y,Chen Z,Chen J

    更新日期：2019-07-01 00:00:00

  • Glutamate-mediated excitotoxicity and neurodegeneration in Alzheimer's disease.

    abstract：:Alzheimer's disease (AD) is the most common form of dementia, accounting for 60-70% of cases in subjects over 65 years of age. Several postulates have been put forward that relate AD neuropathology to intellectual and functional impairment. These range from free-radical-induced damage, through cholinergic dysfunction,...

    journal_title：Neurochemistry international

    pub_type： 杂志文章,评审

    doi：10.1016/j.neuint.2004.03.007

    authors： Hynd MR,Scott HL,Dodd PR

    更新日期：2004-10-01 00:00:00

  • Impaired electro-genesis in skeletal muscle fibers of transgenic Alzheimer mice.

    abstract：:Alzheimer's disease (AD) is characterized by memory decline, but is often associated with non-cognitive symptoms, including muscular dysfunction. In the majority of cases these motor disturbances are seen when other neuro-degenerative disorders such as Parkinson's disease overlap dementia, however these can also be di...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2013.10.014

    authors： Mukhamedyarov MA,Volkov EM,Khaliullina DF,Grigoryev PN,Zefirov AL,Palotás A

    更新日期：2014-01-01 00:00:00

  • Adrenoceptors in brain: cellular gene expression and effects on astrocytic metabolism and [Ca(2+)]i.

    abstract：:Recent in vivo studies have established astrocytes as a major target for locus coeruleus activation (Bekar et al., 2008), renewing interest in cell culture studies on noradrenergic effects on astrocytes in primary cultures and calling for additional information about the expression of adrenoceptor subtypes on differen...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2010.03.019

    authors： Hertz L,Lovatt D,Goldman SA,Nedergaard M

    更新日期：2010-11-01 00:00:00

  • Differential effect of acute reserpine administration on D-1 and D-2 dopaminergic receptor density and function in rat striatum.

    abstract：:In the present study we investigated the effects of a single injection of reserpine on dopamine receptor function in rat striatum. The measurement of adenylate cyclase activity indicated that cAMP formation induced by the selective D-1 agonist SKF 82526 was greatly increased 2 and 20 h after reserpine; in contrast, th...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(89)90010-7

    authors： Liberini P,Nisoli E,Missale C,Memo M,Buonamici M,Rossi A,Spano PF

    更新日期：1989-01-01 00:00:00

  • Molsidomine, a nitric oxide donor, modulates rotational behavior and monoamine metabolism in 6-OHDA lesioned rats treated chronically with L-DOPA.

    abstract：:Some biochemical and histological studies of Parkinson's disease patients' brains and 6-OHDA-lesioned rats suggest that dopaminergic dennervation of the striatum leads to the nitrergic system hypofunction in this structure. Hence, recently the modulation of nitric oxide (NO)- soluble guanylyl cyclase-cyclic GMP signal...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2013.09.021

    authors： Lorenc-Koci E,Czarnecka A,Lenda T,Kamińska K,Konieczny J

    更新日期：2013-12-01 00:00:00

  • Protein S100B release from rat brain slices during and after ischemia: comparison with lactate dehydrogenase leakage.

    abstract：:One hour of ischemia significantly increased protein S100B release from rat brain slices without altering lactate dehydrogenase leakage. Reoxygenation of the ischemic slices, however, increased the levels of these biochemical markers in the medium. Although removal of extracellular Ca(+2) ions from the medium did not ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2005.06.009

    authors： Büyükuysal RL

    更新日期：2005-12-01 00:00:00

  • Electroacupuncture preconditioning-induced neuroprotection may be mediated by glutamate transporter type 2.

    abstract：:Electroacupuncture has been shown to induce a preconditioning effect in the brain. The mechanisms for this protection are not fully elucidated. We hypothesize that this protection is mediated by excitatory amino acid transporters (EAATs) that have been shown to be neuroprotective. To test this hypothesis, two-month ol...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2013.06.017

    authors： Zhu X,Yin J,Li L,Ma L,Tan H,Deng J,Chen S,Zuo Z

    更新日期：2013-10-01 00:00:00

  • Characterization of high affinity and stereospecific [ (3)H ]d- fenfluramine binding to rat brain.

    abstract：:Specific binding of [(3)H]d- fenfluramine , an anorectic agent acting on serotonergic system, was characterized in rat total brain minus cerebellum. The binding was reversible, stereospecific and with high affinity (K(d) about 80 nM). The receptor density in total brain was about 32 pmol/g tissue. Subcellular distribu...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(88)90007-1

    authors： Mennini T,Gobbi M,Taddei C,Garattini S

    更新日期：1988-01-01 00:00:00

  • Effect of hypercholesterolemia and of oxidative stress on the nitric oxide-cGMP pathway.

    abstract：:Soluble guanylyl cyclase (sGC) is a key enzyme of the NO-cGMP pathway which is believed to mediate vasoprotective actions. In cardiovascular diseases such as hypercholesterolemia and atherosclerosis, these important functions of the vascular endothelium are strongly impaired. One of the major reasons for this so-calle...

    journal_title：Neurochemistry international

    pub_type： 杂志文章,评审

    doi：10.1016/j.neuint.2004.03.026

    authors： Francois M,Kojda G

    更新日期：2004-11-01 00:00:00