Abstract：
:Ras homolog enriched in the brain (Rheb) is a homolog of Ras GTPase that regulates cell growth, proliferation, and cell cycle via mammalian target of rapamycin (mTOR). Recently, it has been confirmed that Rheb activation not only promotes cellular proliferation and differentiation but also enhances cellular apoptosis in response to diverse toxic stimuli. However, the function of Rheb in the central nervous system (CNS) is still with limited understanding. To elaborate whether Rheb was involved in CNS injury, we performed a neuroinflammatory model by lipopolysaccharide (LPS) lateral ventral injection in adult rats. Upregulation of Rheb was observed in the brain cortex by performing western blotting and immunohistochemistry. Double immunofluorescent staining demonstrated that Rheb was mainly in active astrocytes and neurons. PCNA and active caspase-3 were upregulated, and co-labeling with Rheb, which indicated that Rheb might be relevant to astrocytic proliferation and neuronal apoptosis following the inflammatory response by LPS-induced. Furthermore, we also found that the expression profiles of cyclinD1 and CDK4 were parallel with that of Rheb in a time-space dependent manner. Finally, knocking down Rheb by siRNA and treatment with rapamycin or lovastatin showed that not only astrocytic proliferation decreased but also neuronal protection. Based on our data, we suggested that Rheb might play an important role in physiological and pathological functions following neuroinflammation caused by LPS, which might provide a potential target to the treatment of neuroinflammation.
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Cao M,Tan X,Jin W,Zheng H,Xu W,Rui Y,Li L,Cao J,Wu X,Cui G,Ke K,Gao Ydoi
10.1016/j.neuint.2013.01.025subject
Has Abstractpub_date
2013-03-01 00:00:00pages
406-17issue
4eissn
0197-0186issn
1872-9754pii
S0197-0186(13)00034-Xjournal_volume
62pub_type
杂志文章abstract：:Acyl-CoA synthetase activity was investigated in membrane fractions from normal and Trembler mouse sciatic nerves. Stearoyl-CoA synthesis in the Trembler assays was always about twice that observed in the control experiments under various conditions of CoA and ATP concentrations. In experiments using varying concentra...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(85)90035-x
更新日期：1985-01-01 00:00:00
abstract：:D1 and D2 receptors are key mediators of dopaminergic signaling in the brain, and since the manifestations of pathologies related to dopamine are different in female and male patients, it is important to analyze if there are sex-related differences in dopaminergic markers. To contribute to the knowledge in this regard...
journal_title：Neurochemistry international
pub_type： 杂志文章
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更新日期：2016-11-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2020.104710
更新日期：2020-05-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(85)90009-9
更新日期：1985-01-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2006.05.005
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2013.08.010
更新日期：2013-11-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(87)90032-5
更新日期：1987-01-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(96)00094-0
更新日期：1997-06-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(90)90032-o
更新日期：1990-01-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(89)90114-9
更新日期：1989-01-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2006.06.008
更新日期：2006-12-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/j.neuint.2009.01.025
更新日期：2009-07-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(95)00093-3
更新日期：1996-04-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(00)00106-6
更新日期：2001-04-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2007.06.013
更新日期：2008-01-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(94)90110-4
更新日期：1994-04-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2009.03.015
更新日期：2009-09-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2016.12.005
更新日期：2017-03-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2010.10.018
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journal_title：Neurochemistry international
pub_type： 杂志文章
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2008.02.008
更新日期：2008-06-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(86)90148-8
更新日期：1986-01-01 00:00:00
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journal_title：Neurochemistry international
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(01)00034-1
更新日期：2001-10-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(98)00013-8
更新日期：1998-08-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(93)90089-n
更新日期：1993-08-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(88)90165-9
更新日期：1988-01-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(84)90126-8
更新日期：1984-01-01 00:00:00
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journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/j.neuint.2014.02.007
更新日期：2014-04-01 00:00:00
abstract：:Rapid eye movement sleep deprivation is associated with an increase in Na-K ATPase activity. In order to understand the possible biochemical mechanism of this increase, the kinetics of Na-K ATPase was studied. Although the enzyme activity increased after the deprivation, the catalytic efficiency of the enzyme remained...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(99)00121-7
更新日期：2000-03-01 00:00:00
