Abstract：
CONTEXT:Platelet-derived microparticles (PDMPs) probably function in hemostasis, thrombosis, inflammation, and transfusion-related immunomodulation. OBJECTIVE:To compare PDMP levels of leukocyte-filtered and unfiltered whole blood during storage. DESIGN:Ten whole blood donations were collected and processed. Half of each collection was filtered, half remained unfiltered, and both halves were measured for red cell, white cell, and platelet (PLT) content before storage. Samples were drawn on days 0, 1, 2, 3, 5, 7, 14, 21, 28, and 35 and analyzed by flow cytometry. RESULTS:Leukocyte filtration lowered prestorage PDMP and PLT counts by an average of 72% and 99%, respectively. Prestorage PDMP counts were 123 +/- 51/microL in unfiltered whole blood supernatant versus 34 +/- 18/microL after filtration. Prestorage PLT counts were 190 +/- 49/microL in unfiltered whole blood supernatant versus 2 +/- 4/microL after filtration. Moreover, PDMP and PLT counts in filtered whole blood remained low throughout storage, typically below 100/microL. In contrast, unfiltered whole blood PDMP- and PLT-gated events increased approximately 2 log during storage, with the peak number of PLT-gated events tending to coincide with the peak number of PDMP-gated events (4 donors) or to come after the peak number of PDMP-gated events (6 donors). CONCLUSIONS:Leukocyte filtration of whole blood lowers prestorage PDMP and PLT counts. Platelet-derived microparticle and PLT counts remain low throughout 35 days of storage. In contrast, PDMP- and PLT-gated events increase significantly in unfiltered whole blood. The nature of PLT-gated events in stored blood warrants further investigation.
journal_name
Arch Pathol Lab Medjournal_title
Archives of pathology & laboratory medicineauthors
Sugawara A,Nollet KE,Yajima K,Saito S,Ohto Hdoi
10.1043/1543-2165-134.5.771subject
Has Abstractpub_date
2010-05-01 00:00:00pages
771-5issue
5eissn
0003-9985issn
1543-2165pii
10.1043/1543-2165-134.5.771journal_volume
134pub_type
杂志文章abstract：:A survey of manpower supply and need in academic pathology departments was conducted by the Joint Task Force on Pathology Manpower of the College of American Pathologists, the American Society of Clinical Pathologists, and the Association of Pathology Chairmen by means of questionnaires distributed to chairmen and fac...
journal_title：Archives of pathology & laboratory medicine
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doi：
更新日期：1985-10-01 00:00:00
abstract：CONTEXT:Although the value of pharmacogenomics to improve patient outcomes has become increasingly clear, adoption in medical practice has been slow, which can be attributed to several factors, including complicated and expensive testing procedures and required equipment, lack of training by private practice physicians...
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journal_title：Archives of pathology & laboratory medicine
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journal_title：Archives of pathology & laboratory medicine
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journal_title：Archives of pathology & laboratory medicine
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更新日期：1980-06-01 00:00:00
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更新日期：1988-01-01 00:00:00
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更新日期：1979-02-01 00:00:00
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journal_title：Archives of pathology & laboratory medicine
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更新日期：1988-02-01 00:00:00
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journal_title：Archives of pathology & laboratory medicine
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journal_title：Archives of pathology & laboratory medicine
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更新日期：1976-12-01 00:00:00
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journal_title：Archives of pathology & laboratory medicine
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journal_title：Archives of pathology & laboratory medicine
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doi：
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journal_title：Archives of pathology & laboratory medicine
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doi：
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journal_title：Archives of pathology & laboratory medicine
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doi：
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doi：
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