Abstract：
CONTEXT:Although the value of pharmacogenomics to improve patient outcomes has become increasingly clear, adoption in medical practice has been slow, which can be attributed to several factors, including complicated and expensive testing procedures and required equipment, lack of training by private practice physicians, and reluctance of both private and commercial payers to reimburse for such testing. OBJECTIVES:To evaluate a fully automated molecular detection system for human genotyping assays, starting with anticoagulated whole blood samples, and to perform all sample preparation, assay, and analysis steps automatically with actionable results reported by the system's software. DESIGN:The genotypes of 254 random individuals were determined by performing bidirectional DNA sequencing, and that information was used to statistically train the imaging software of the automated molecular detection system to distinguish the 3 possible genotypes (ie, homozygous wild type, heterozygous, and homozygous mutant) at each of 3 different loci (CYP2C9*2, CYP2C9*3, and VKORC1). RESULTS:The resulting software algorithm was able to correctly identify the genotypes of all 254 individuals (100%) evaluated without any further user analysis. CONCLUSIONS:The EncompassMDx workstation (Rheonix, Inc, Ithaca, New York) is a molecular detection system that can automatically determine the genotypes of individuals in an unattended manner. Considerably less technical expertise was required to achieve results identical to those obtained using more complex, time-consuming, and expensive bidirectional DNA sequencing. This optimized system may dramatically simplify and reduce the costs of pharmacogenomics testing, thus leading to more-widespread use.
journal_name
Arch Pathol Lab Medjournal_title
Archives of pathology & laboratory medicineauthors
Spizz G,Chen Z,Li P,McGuire IC,Klimkiewicz P,Zysling D,Yasmin R,Hungerford W,Thomas B,Wilding G,Mouchka G,Young L,Zhou P,Montagna RAdoi
10.5858/arpa.2014-0059-OAsubject
Has Abstractpub_date
2015-06-01 00:00:00pages
805-11issue
6eissn
0003-9985issn
1543-2165journal_volume
139pub_type
杂志文章abstract：CONTEXT:Previous studies have shown that paraproteins caused spurious results on individual analytes including total bilirubin (TBIL), direct bilirubin (DBIL), or HDL-cholesterol (HDL-C). Studies demonstrating paraprotein interferences with multiple analytes measured by different analyzers have not been reported. OBJE...
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abstract：:By means of an electrofocusing technique, the serum specimens of pregnant women routinely revealed three bands of alkaline phosphatase (ALP) that were released by placental syncytiotrophoblast, germ cell (ovary), and fetal intestine at pl of 4.73, 4.65, and 4.55, respectively. When defining reference ranges for ostens...
journal_title：Archives of pathology & laboratory medicine
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doi：
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journal_title：Archives of pathology & laboratory medicine
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journal_title：Archives of pathology & laboratory medicine
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journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1993-05-01 00:00:00
abstract：OBJECTIVE:To evaluate three fluorescent chitin stains for detecting microsporidia spores in specimens from acquired immunodeficiency syndrome (AIDS) patients with chronic diarrhea. METHODS:We compared the Fungifluor, Calcofluor White, and Fungiqual A fluorochrome stains for identifying Enterocytozoon bieneusi and Sept...
journal_title：Archives of pathology & laboratory medicine
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doi：
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journal_title：Archives of pathology & laboratory medicine
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journal_title：Archives of pathology & laboratory medicine
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journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,多中心研究
doi：
更新日期：1993-10-01 00:00:00
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journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
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journal_title：Archives of pathology & laboratory medicine
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journal_title：Archives of pathology & laboratory medicine
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doi：
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journal_title：Archives of pathology & laboratory medicine
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