Abstract：
CONTEXT:Nucleophosmin 1 (NPM1) is the most commonly mutated gene in acute myeloid leukemia. Detection of NPM1 mutations is useful for stratifying patients for therapy, predicting prognosis, and assessing for minimal residual disease. Several methods have been developed to rapidly detect NPM1 mutations in genomic DNA and/or messenger RNA specimens. OBJECTIVE:To directly compare a quantitative real-time polymerase chain reaction (qPCR) assay with a widely used capillary electrophoresis assay for detecting NPM1 mutations. DESIGN:We adopted and modified a qPCR assay designed to detect the 6 most common NPM1 mutations and performed the assay in parallel with capillary electrophoresis assay in 207 bone marrow aspirate or peripheral blood samples from patients with a range of hematolymphoid neoplasms. RESULTS:The qPCR assay demonstrated a higher analytical sensitivity than the capillary electrophoresis 1/1000 versus 1/40, respectively. The capillary electrophoresis assay generated 10 equivocal results that needed to be repeated, whereas the qPCR assay generated only 1 equivocal result. After test conditions were optimized, the qPCR and capillary electrophoresis methods produced 100% concordant results, 85 positive and 122 negative. CONCLUSIONS:Given the higher analytical sensitivity and specificity of the qPCR assay, that assay is less likely to generate equivocal results than the capillary electrophoresis assay. Moreover, the qPCR assay is quantitative, faster, cheaper, less prone to contamination, and well suited for monitoring minimal residual disease.
journal_name
Arch Pathol Lab Medjournal_title
Archives of pathology & laboratory medicineauthors
Barakat FH,Luthra R,Yin CC,Barkoh BA,Hai S,Jamil W,Bhakta YI,Chen S,Medeiros LJ,Zuo Zdoi
10.5858/2010-0490-OAR1subject
Has Abstractpub_date
2011-08-01 00:00:00pages
994-1000issue
8eissn
0003-9985issn
1543-2165pii
10.1043/2010-0490-OAR1journal_volume
135pub_type
杂志文章abstract：:Atherosclerosis can be defined as a disease process that occurs when the influx and deposition of cholesterol into the arterial wall exceed the egress of cholesterol from the arterial wall. This process is characterized by early deposition of cholesterol in and around arterial wall cells. The cholesterol is derived fr...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：
更新日期：1983-08-01 00:00:00
abstract：:We report the case of a primary breast rhabdomyosarcoma in a 51-year-old white woman. The tumor occupied the upper outer quadrant of the right breast. There was no association with the underlying pectoral muscle, and the patient was free of any other benign or malignant neoplastic processes outside the breast. Microsc...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：
更新日期：1998-08-01 00:00:00
abstract：:We report a water-clear cell adenoma of the parathyroid gland, a lesion which to our knowledge has not been described previously. Like its rare but well-described hyperplastic counterpart, water-clear cell hyperplasia, this adenoma is composed of cells with abundant foamy-to-granular cytoplasm and mild nuclear pleomor...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:Seven malignant peripheral lung tumors that were diagnosed using light microscopy as large-cell carcinomas or as epidermoid or adenocarcinomas were studied by light and electron microscopic histochemistry. All tumors contained numerous dense-core granules. The cells were joined by desmosomes and contained well-develop...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:We report a case of fatal fibrosing peritonitis in a 28-year-old drug abuser. The extensive fibrosis, which was limited to the abdominal cavity, contained birefringent material. Silica was identified by spectrophotometric analysis. Silica is a component of talc (magnesium silicate) used as a drug filler. Relatively sm...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1985-08-01 00:00:00
abstract：CONTEXT:Fine-needle aspiration has become an accepted and cost-effective procedure for rapid diagnosis of thyroid lesions. The routine use of fine-needle aspiration has reduced the rate of unnecessary surgery for thyroid nodules. OBJECTIVES:To determine the accuracy of fine-needle aspiration biopsy diagnosis and to di...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/0003-9985(2001)125<0484:AOFNAO>2.0.CO;2
更新日期：2001-04-01 00:00:00
abstract：CONTEXT:The results of studies among patients with antithrombin deficiency have suggested that the use of warfarin will increase the level of antithrombin. OBJECTIVE:To reevaluate the effect of warfarin on antithrombin levels using an automated amidolytic method in current use. DESIGN:Antithrombin levels were measure...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.5858/arpa.2013-0065-OA
更新日期：2014-07-01 00:00:00
abstract：CONTEXT:Differences in participant responses for ThinPrep (TP) and non-ThinPrep (NTP) preparations for gastrointestinal cytology challenges, which circulated in the College of American Pathologists Interlaboratory Comparison Program in Nongynecologic Cytology (CAP NGC), may help to identify performance variations betwe...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/2009-0326-CP.1
更新日期：2010-08-01 00:00:00
abstract：:This study was undertaken to assess the specificity of split renal basement membranes in hereditary nephritis (HN). Thirteen specimens from eight patients with HN were mixed in a random fashion with specimens from control patients with either idiopathic nephrotic syndrome or various forms of glomerulonephritis and wit...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1976-09-01 00:00:00
abstract：:The costs of producing quality medical care and the proper allocation of resources to assure quality are major contemporary concerns. Minimalists attempt to improve quality and lower costs through the application of the theory, "less is more." Bureaucracies make the same attempt through the denial of payments for "unn...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
abstract：:Teratoid hepatoblastoma is a rare histologic subtype accounting for 4% to 10% of all hepatoblastomas and is characterized by the presence of divergent differentiation, including squamous, mucinous, melanocytic, cartilaginous, osseous, skeletal muscle, and neural elements. It is postulated that the teratoid elements mi...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.5858/arpa.2012-0212-CR
更新日期：2012-08-01 00:00:00
abstract：:Pathologic examination of an excisional biopsy specimen of a subepidermal nodule from the nasal tip of a 37-year-old black man demonstrated papillae covered by eosinophilic columnar epithelial cells, suggesting cylindric cell papilloma or well-differentiated papillary adenocarcinoma of nasal mucosa. The patient did no...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/0003-9985(2003)127
更新日期：2003-02-01 00:00:00
abstract：:Findings of a histochemical and ultrastructural study of the mucogenic cells of congenital adenomatoid malformation of lung indicate that these cells share features with goblet cells of bronchial or intestinal origin and do not indicate a similarity to normal gastric epithelium or glands, as has been previously sugges...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1979-05-01 00:00:00
abstract：CONTEXT:- Colonic inertia is a debilitating form of primary chronic constipation with unknown etiology and diagnostic criteria, often requiring pancolectomy. We have occasionally observed massively enlarged submucosal ganglia containing at least 20 perikarya, in addition to previously described giant ganglia with great...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.5858/arpa.2016-0551-OA
更新日期：2018-02-01 00:00:00
abstract：:We describe a unique case of simultaneous signet-ring histiocytes containing polyvinylpyrrolidone (mucicarminophilic histiocytosis) and hyperplastic mesothelial cells within a single supraclavicular lymph node. Both types of cells expanded the nodal sinuses and were initially suspected of being metastatic carcinoma. H...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:A 67-year-old man, with multiple skin lesions that appeared over 2 years, had biopsies that disclosed granulomatous dermatitis with associated small yeasts. The urinary antigen test results were negative for Histoplasma infection; cultures from the biopsies did not grow any fungi or other potential pathogens. The ches...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.5858/2010-0588-CRR.1
更新日期：2011-09-01 00:00:00
abstract：OBJECTIVE:To design, develop, and implement a prototype medical event-reporting system for use in transfusion medicine to improve transfusion safety by studying incidents and errors. METHODS:The IDEALS concept of design was used to identify specifications for the event-reporting system, and a Delphi and subsequent nom...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:Malignant granular cell tumor is a rare neoplasm that has been reported to occur at various sites. Although there are no reliable histologic criteria to differentiate the benign from the malignant form of granular cell tumor, in many reported cases cellular pleomorphism and the presence of mitotic activity suggested a...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
abstract：OBJECTIVE:To compare the ability of the Vitek GPS-TB card with disk diffusion testing for determining the susceptibility of enterococci to vancomycin. DESIGN:Vitek susceptibility testing was performed using the GPS-TB card and software version R05.03. Disk diffusion susceptibility testing was performed according to Na...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/0003-9985(1999)123<0622:COTVGT>2.0.CO;2
更新日期：1999-07-01 00:00:00
abstract：CONTEXT:In Thailand until 2005 there had been no external quality assessment scheme at the national level for blood coagulation tests. Only a few laboratories had an external quality assessment for these tests. In the year 2005, the Thailand National External Quality Assessment Scheme for Blood Coagulation was founded....
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/1543-2165-133.1.72
更新日期：2009-01-01 00:00:00
abstract：:Myocardium as emboli is a decidedly rare phenomenon. Only three such examples were identified among 8033 consecutive autopsies performed over a ten-year period, 1976 to 1985, an incidence of 0.037%. In two of these three cases, myocardial emboli occurred in the systemic (coronary) circulation. The source of emboli was...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
abstract：CONTEXT.—:The incidence of anal cancer in the United States is on the rise in high-risk populations. The anal Papanicolaou test (APT) is advocated as a screening tool, in addition to digital rectal examination and high-resolution anoscopy. OBJECTIVE.—:To review our experience and the current literature to create, in c...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.5858/arpa.2017-0242-RA
更新日期：2019-01-01 00:00:00
abstract：:Paraneoplastic disorders (PNDs) are remote effects of tumors that are unrelated to the size, location, metastases, or physiological activities of mature tissue of origin. Some of these disorders, such as fever and anemia, have been known for a long time. Other disorders, such as lymphopenia and low serum cholesterol l...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：CONTEXT:Basal-like breast carcinoma (BLBC) is a distinct molecular subtype of breast carcinoma identified through gene expression profiling studies. OBJECTIVE:To provide the clinical background, the histologic profile, and the immunohistochemical profile of these tumors and discuss the current knowledge of their molec...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.5858/arpa.2012-0439-RA
更新日期：2014-01-01 00:00:00
abstract：:We report the case of a patient treated with a combination of fludarabine and cyclophosphamide after suffering from B-cell chronic lymphocytic leukemia for 10 years. Three months after treatment, the patient presented with an unusual association, not previously reported in the literature: Richter syndrome (monotypic E...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/1543-2165(2006)130[1227:CEVLBL]2.0.CO;2
更新日期：2006-08-01 00:00:00
abstract：CONTEXT:Mutations of the proto-oncogene B-raf (BRAF) have been detected in melanocytic lesions and papillary carcinomas of the thyroid, and identification of these mutations could be useful in resolving some diagnostic problems. OBJECTIVE:To develop a method to evaluate mutations of BRAF that could provide results muc...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：10.1043/1543-2165(2007)131[1361:BMIMLA]2.0.CO;2
更新日期：2007-09-01 00:00:00
abstract：:A benign chondrolipomatous breast tumor occurred in a 66-year-old woman. The preoperative diagnosis, based on mammography and xeroradiography, was fibroadenoma. Grossly, the demarcated lesion resembled a fibroadenoma with islands of cartilage projecting from its cut surface. Histologically, it was composed of benign m...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:Patients with xeroderma pigmentosum (XP) experience cutaneous and ocular abnormalities, including neoplasia of epithelial and melanocytic cellular origin. Black patients with XP display more severe ocular abnormalities and neoplasia. We report the clinicopathologic findings in two black young children with XP who pres...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：
更新日期：1991-09-01 00:00:00
abstract：:Questionnaires sent to representative medicolegal jurisdictions in the United States showed that medicolegal necropsies comprise a substantial proportion of the total number of autopsies performed. Qualitative differences between customary hospital autopsies and their forensic counterparts include autopsy bias, the im...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章
doi：
更新日期：1984-06-01 00:00:00
abstract：:Succinate dehydrogenase (SDH)-deficient renal cell carcinoma is a recently recognized distinct subtype of renal cell carcinoma in the 2016 World Health Organization classification. It is associated with SDH gene germline mutations, which also cause paraganglioma/pheochromocytoma, gastrointestinal stromal tumor, and pi...
journal_title：Archives of pathology & laboratory medicine
pub_type： 杂志文章,评审
doi：10.5858/arpa.2018-0024-RS
更新日期：2019-05-01 00:00:00
