当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Novel fluorescent cycloheximide derivatives for the imaging of protein synthesis.

Novel fluorescent cycloheximide derivatives for the imaging of protein synthesis.

Abstract：

:Cycloheximide (CHX) is one of the most interesting protein synthesis inhibitors. For this reason, fluorescent derivatives of CHX could find useful applications in cell biology. We report the successful synthesis of a set of novel fluorescent derivatives of CHX. The effect of different functional groups on the biological activity of CHX was studied upon their modification through suitable strategies, i.e., acetylation of the hydroxyl group and reductive amination of the ketone group. The first route induced a complete loss of biological activity, while the second approach allowed a retained inhibition of protein synthesis, as demonstrated by in vitro translation assays. Various fluorescent dyes for reductive amination were tested (i.e., ANTS, APTS, and Rhodamine-123), and the success of the syntheses was demonstrated by diverse analytical techniques. Cycloheximide labeling with fluorescent dyes is a promising approach for developing fluorescence reporters for various applications, both in vitro (fluorescence spectroscopy) and in vivo (live imaging).

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Paoletti F,Ainger K,Donati I,Scardigli R,Vetere A,Cattaneo A,Campa C

doi

10.1016/j.bbrc.2010.04.075

subject

Has Abstract

pub_date

2010-05-28 00:00:00

pages

258-64

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(10)00744-8

journal_volume

396

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Localization of phosphoprotein PP 105 in cell lines of various species.

    abstract：:The cellular location of phosphoprotein pp 105 was determined in various mouse cell lines with rabbit anti mouse pp 105 serum. Immunofluorescence was predominantly observed in the nucleoli in addition to a diffuse but weaker fluorescence of the whole nucleus. Cell surface fluorescence was obtained only with cells grow...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)90387-x

    authors： Pfeifle J,Anderer FA

    更新日期：1983-10-14 00:00:00

  • Connecting CuA with metal centers of heme a, heme a3, CuB and Zn by pathways with hydrogen bond as the bridging element in cytochrome c oxidase.

    abstract：:Pathways formed of delocalized π-electron systems and polar groups of polypeptide chains bridged by hydrogen bonds are referred as π-H pathways. Suitable for electron transfer, these pathways in cytochrome c oxidase connect CuA, the source of electrons distributed in cytochrome c oxidase, with the metal centers, heme ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.01.083

    authors： Ramasarma T,Vaigundan D

    更新日期：2019-03-05 00:00:00

  • Functional characterization of an androgen response element in the first intron of the C3(1) gene of prostatic binding protein.

    abstract：:We demonstrate that the 204 bp intronic gene fragment of C3(1), which has a specific in vitro affinity for the androgen receptor, is able to confer androgen responsiveness to a heterologous promoter. This characteristic is completely destroyed by a single G----T substitution, affecting a 5'-TGTTCT-3' element that clos...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91534-9

    authors： Claessens F,Celis L,Peeters B,Heyns W,Verhoeven G,Rombauts W

    更新日期：1989-10-31 00:00:00

  • Hydrogen from intestinal bacteria is protective for Concanavalin A-induced hepatitis.

    abstract：:It is well known that some intestinal bacteria, such as Escherichia coli, can produce a remarkable amount of molecular hydrogen (H(2)). Although the antioxidant effects of H(2) are well documented, the present study examined whether H(2) released from intestinally colonized bacteria could affect Concanavalin A (ConA)-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.06.024

    authors： Kajiya M,Sato K,Silva MJ,Ouhara K,Do PM,Shanmugam KT,Kawai T

    更新日期：2009-08-21 00:00:00

  • The Mycobacterium tuberculosis transcriptional repressor EthR is negatively regulated by Serine/Threonine phosphorylation.

    abstract：:Recent efforts have underlined the role of Serine/Threonine Protein Kinases (STPKs) in growth, pathogenesis and cell wall metabolism in mycobacteria. Herein, we demonstrated that the Mycobacterium tuberculosis EthR, a transcriptional repressor that regulates the activation process of the antitubercular drug ethionamid...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.03.074

    authors： Leiba J,Carrère-Kremer S,Blondiaux N,Dimala MM,Wohlkönig A,Baulard A,Kremer L,Molle V

    更新日期：2014-04-18 00:00:00

  • miR-410-3p promotes prostate cancer progression via regulating PTEN/AKT/mTOR signaling pathway.

    abstract：:Prostate cancer has become one of commonest urologic tumors in male. In recent years, miRNAs are continually attracting attentions of researchers for their special regulatory function in human cancers. Previous study has revealed that miR-410 acts as a biomarker for the diagnosis of prostate cancer. Whereas, the speci...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.06.176

    authors： Zhang Y,Zhang D,Lv J,Wang S,Zhang Q

    更新日期：2018-09-18 00:00:00

  • Reduced tumour necrosis factor-induced cytotoxicity by inhibitors of the arachidonic acid metabolism.

    abstract：:The mechanism of tumour necrosis factor-mediated cytotoxicity was investigated by using various inhibitors of arachidonic acid metabolism. Phospholipase A2 inhibitors with different modes of action interfered with the cytotoxic action of TNF, whereas phospholipase C inhibitors did not. Neither cyclooxygenase nor lipox...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90429-3

    authors： Suffys P,Beyaert R,Van Roy F,Fiers W

    更新日期：1987-12-16 00:00:00

  • Characterization of a Smad motif similar to Drosophila mad in the mouse Msx 1 promoter.

    abstract：:Mouse Msx 1 gene, orthologous of the Drosophila msh, is involved in several developmental processes. BMP family members are major proteins in the regulation of Msx 1 expression. BMP signaling activates Smad 1/5/8 proteins, which associate to Smad 4 before translocating to the nucleus. Analysis of Msx 1 promoter reveal...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6502

    authors： Alvarez Martinez CE,Binato R,Gonzalez S,Pereira M,Robert B,Abdelhay E

    更新日期：2002-03-01 00:00:00

  • Protein misfolding, mitochondrial dysfunction and muscle loss are not directly dependent on soluble and aggregation state of mSOD1 protein in skeletal muscle of ALS.

    abstract：:Mutant superoxide dismutase 1 (mSOD1) is often found as aggregates at the outer-membrane of mitochondria in motor neurons of various mouse models and familial amyotrophic lateral sclerosis (f-ALS) patients. It has been postulated that disruption of mitochondrial function by physical association of misfolded mSOD1 aggr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.12.126

    authors： Wei R,Bhattacharya A,Chintalaramulu N,Jernigan AL,Liu Y,Van Remmen H,Chaudhuri AR

    更新日期：2012-01-27 00:00:00

  • Effect of botulinum D toxin on neutrophils.

    abstract：:Activated botulinum D toxin ADP-ribosylates a 22 kDa molecular weight protein in homogenates obtained by sonication of a suspension of rabbit peritoneal neutrophils. The ADP-ribosylation catalyzed by activated botulinum D toxin is inhibited in homogenates obtained from cells pretreated with the toxin, suggesting that ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80129-3

    authors： Mege JL,Volpi M,Becker EL,Sha'afi RI

    更新日期：1988-04-29 00:00:00

  • The WNT inhibitor APCDD1 sustains the expression of β-catenin during the osteogenic differentiation of human dental follicle cells.

    abstract：:In hair follicle cells APCDD1 inhibits the canonical WNT/β-Catenin pathway and its inactivation is associated with an autosomal dominant form of hair loss. We analyzed the role of APCDD1 for the osteogenic differentiation in dental follicle cells (DFCs) and identified a new and surprising function. Contrarily to hair ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.12.107

    authors： Viale-Bouroncle S,Klingelhöffer C,Ettl T,Morsczeck C

    更新日期：2015-02-13 00:00:00

  • Effect of a mechanical tension on the hydration of DNA in fibres.

    abstract：:Fiber X-ray diffraction and measurement of fibre dimensions yield information about the effects of a mechanical tension on hydration of DNA in fibres. At a given relative humidity, the mechanical tension changes the DNA conformation but does not modify the number of water molecules associated to a nucleotide. The numb...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)92352-x

    authors： Harmouchi M,Albiser G,Premilat S

    更新日期：1992-10-15 00:00:00

  • Purification of a novel serine proteinase inhibitor from the skeletal muscle of white croaker (Argyrosomus argentatus).

    abstract：:A novel serine proteinase inhibitor has been purified to homogeneity from the skeletal muscle of white croaker (Argyrosomus argentatus). The purification was carried out by ammonium sulfate fractionation, DEAE-Sephacel, heating treatment followed by column chromatographies on SP-Sepharose, Sephadex G-150 and gel-filtr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2803

    authors： Cao MJ,Osatomi K,Matsuda R,Ohkubo M,Hara K,Ishihara T

    更新日期：2000-06-07 00:00:00

  • Monoterpenoid perillyl alcohol impairs metabolic flexibility of Candida albicans by inhibiting glyoxylate cycle.

    abstract：:The metabolic pathway such as glyoxylate cycle (GC) enables Candida albicans, to survive under glucose deficient conditions prevalent in the hostile niche. Thus its key enzymes (Isocitrate lyase; ICL and malate synthase; MLS) represent attractive targets against C. albicans. We have previously reported the antifungal ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.11.064

    authors： Ansari MA,Fatima Z,Ahmad K,Hameed S

    更新日期：2018-01-01 00:00:00

  • Trichosanthin affects HSV-1 replication in Hep-2 cells.

    abstract：:Trichosanthin (TCS) is a type I ribosome-inactivating protein that inhibits the replication of both human immunodeficiency virus type 1 (HIV-1) and herpes simplex virus type 1 (HSV-1). The mechanism of inhibition is not clear. This investigation explored the effects of TCS on the stages of HSV-1 infection in Hep-2 cel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.10.080

    authors： He DX,Tam SC

    更新日期：2010-11-26 00:00:00

  • Characterization of ADAR1-mediated modulation of gene expression.

    abstract：:Conversion of adenosine into inosine in RNA molecules constitutes an important post-transcriptional mechanism for generating transcript diversity and is catalyzed by adenosine deaminases acting on RNA (ADARs). Intriguingly, we observed that the editing enzyme ADAR1 enhances reporter gene expression in a cellular, plas...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.09.109

    authors： Gommans WM,Maas S

    更新日期：2008-12-05 00:00:00

  • Human erythrocyte ankyrin, a cytoskeleton component, generates the p57 membrane proteinase which cleaves C3, the third component of complement.

    abstract：:Human erythrocytes express a p57 membrane serine proteinase which cleaves C3, the third component of complement. Amino acid analysis of the first 20 N-terminal residues of the purified p57 proteinase demonstrated 100% homology with residues 910-929 of erythrocyte ankyrin, a sequence localized in its Mr = 62 kDa fragme...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2481

    authors： Hermann J,Barel M,Frade R

    更新日期：1994-10-28 00:00:00

  • Decreased contraction of glycated collagen lattices coincides with impaired matrix metalloproteinase production.

    abstract：:Nonenzymatic glycation of extracellular matrix (ECM) proteins is increased in diabetes mellitus and aging and triggers cellular events leading to an imbalance in ECM homeostasis. We studied the influence of collagen glycation on matrix metalloproteinase production by dermal fibroblasts using the model of lattice cultu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1519

    authors： Rittié L,Berton A,Monboisse JC,Hornebeck W,Gillery P

    更新日期：1999-10-22 00:00:00

  • Protein kinase C inhibits autophagy and phosphorylates LC3.

    abstract：:During autophagy, the microtubule-associated protein light chain 3 (LC3), a specific autophagic marker in mammalian cells, is processed from the cytosolic form (LC3-I) to the membrane-bound form (LC3-II). In HEK293 cells stably expressing FLAG-tagged LC3, activation of protein kinase C inhibited the autophagic process...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.04.030

    authors： Jiang H,Cheng D,Liu W,Peng J,Feng J

    更新日期：2010-05-14 00:00:00

  • TopBP1 associates with NBS1 and is involved in homologous recombination repair.

    abstract：:TopBP1 is involved in DNA replication and DNA damage checkpoint. Recent studies have demonstrated that TopBP1 is a direct positive effecter of ATR. However, it is not known how TopBP1 recognizes damaged DNA. Here, we show that TopBP1 formed nuclear foci after exposure to ionizing radiation, but such TopBP1 foci were a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.08.086

    authors： Morishima K,Sakamoto S,Kobayashi J,Izumi H,Suda T,Matsumoto Y,Tauchi H,Ide H,Komatsu K,Matsuura S

    更新日期：2007-11-03 00:00:00

  • Hydrolysis of leucine enkephalin in the nasal cavity of the rat--a possible factor in the low bioavailability of nasally administered peptides.

    abstract：:In order to investigate the utility of intranasal administration of peptides for systemic medication, the nasal absorption of the model peptide, leucine enkephalin (Tyr-Gly-Gly-Phe-Leu), was studied in the rat. At a concentration of 60 micrograms/ml in Ringer's buffer the pentapeptide was found to undergo, extensive h...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91224-0

    authors： Hussain A,Faraj J,Aramaki Y,Truelove JE

    更新日期：1985-12-31 00:00:00

  • Cholecystokinin elevates cytosolic calcium in small cell lung cancer cells.

    abstract：:The ability of cholecystokinin (CCK) to elevate intracellular Ca2+ levels in small cell lung cancer cells was investigated using the fluorescent Ca2+ indicator Fura 2. CCK-8 elevated the cytosolic Ca2+ levels in cell line NCI-H345 in a dose dependent manner. Nanomolar concentration of CCK-8 elevated cytosolic Ca2+ lev...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92180-3

    authors： Staley J,Fiskum G,Moody TW

    更新日期：1989-08-30 00:00:00

  • Retinoic acid switches differential expression of FGF8 isoforms in LNCaP cells.

    abstract：:Retinoic acid (RA) is described as an inhibitor of prostate cancer cell growth. We utilized reverse transcription-polymerase chain reaction (RT-PCR) to analyze expression of different isoforms of fibroblast growth factor 8 (FGF8) in response to RA. Results in the prostate cancer cell line LNCaP show that whereas overa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2740

    authors： Brondani V,Hamy F

    更新日期：2000-05-27 00:00:00

  • A cytoplasmic nickel-iron hydrogenase with high specific activity from Desulfovibrio multispirans sp. N., a new species of sulfate reducing bacterium.

    abstract：:A hydrogenase from a new species of sulfate reducing bacterium has been isolated and characterized. In contrast to other hydrogenases isolated from Desulfovibrio, this enzyme is found in the cytoplasmic fraction rather than in the periplasm. The specific activity of the enzyme, as measured in the hydrogen evolution as...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91386-x

    authors： Czechowski MH,He SH,Nacro M,DerVartanian DV,Peck HD Jr,LeGall J

    更新日期：1984-12-28 00:00:00

  • Identification of Gliotoxin isolated from marine fungus as a new pyruvate kinase M2 inhibitor.

    abstract：:Pyruvate kinase M2 (PKM2) functions as an important rate-limiting enzyme of aerobic glycolysis that is involved in tumor initiation and progression. However, there are few studies on effective PKM2 inhibitors. Gliotoxin is a marine-derived fungal secondary metabolite with multiple biological activities, including immu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.05.139

    authors： Tang W,Liu ZL,Mai XY,Qi X,Li DH,Gu QQ,Li J

    更新日期：2020-07-30 00:00:00

  • 2-Deoxy-d-glucose increases GFAT1 phosphorylation resulting in endoplasmic reticulum-related apoptosis via disruption of protein N-glycosylation in pancreatic cancer cells.

    abstract：:The glycolytic inhibitor 2-deoxy-d-glucose (2DG) causes energy starvation, affecting cell viability in a wide range of cancer cell lines. To determine the action of 2DG in pancreatic cancer, we performed proteomic analysis of pancreatic cancer cell line after 2DG treatment. Eighty proteins showed differential expressi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.05.041

    authors： Ishino K,Kudo M,Peng WX,Kure S,Kawahara K,Teduka K,Kawamoto Y,Kitamura T,Fujii T,Yamamoto T,Wada R,Naito Z

    更新日期：2018-06-27 00:00:00

  • Berberine alleviates hepatic lipid accumulation by increasing ABCA1 through the protein kinase C δ pathway.

    abstract：:Abnormal lipid metabolism may contribute to the pathogenesis of non-alcoholic steatohepatitis (NASH). The ATP-binding cassette transporter A1 (ABCA1) protein mediates the transport of cholesterol and phospholipids from cells to apolipoprotein A-I (apoA-I) to generate nascent HDL particles. Previous studies revealed th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.03.003

    authors： Liang H,Wang Y

    更新日期：2018-04-06 00:00:00

  • Degradation of dynorphin-(1-13) and dynorphin-(1-17) by the neuroblastoma cell membrane. Evidence for the involvement of a cysteine protease.

    abstract：:The membrane of mouse neuroblastoma N-18 cells degraded dynorphin-(1-13), dynorphin-(1-17), and Leu-enkephalin. The degradation of the former two peptides was inhibited strongly by N-ethylmaleimide, moderately by diisopropylphosphorofluoridate and phosphoramidon, and slightly by bestatin. When Leu-enkephalin was the s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)91095-8

    authors： Satoh M,Yokosawa H,Ishii S

    更新日期：1986-10-15 00:00:00

  • Cdc45 degradation during differentiation and apoptosis.

    abstract：:Cell division cycle protein 45 (Cdc45) is crucial for the initiation as well as the elongation process of eukaryotic DNA replication. Our findings suggested that the Cdc45 protein is ubiquitylated and degraded by the proteasome pathway in human cells. Firstly, the fate of Cdc45 protein after induction of terminal diff...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.08.069

    authors： Pollok S,Grosse F

    更新日期：2007-11-03 00:00:00

  • Generation of cloned calves and transgenic chimeric embryos from bovine embryonic stem-like cells.

    abstract：:Bovine embryonic stem-like cells (ES-like cells) appear to maintain a normal diploid karyotype indefinitely during culture in vitro and to express marker proteins that are characteristic of ES cells from mice, namely, alkaline phosphatase (AP), stage-specific embryonic antigen-1 (SSEA-1), STAT-3, and Oct 4. After prol...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)01536-5

    authors： Saito S,Sawai K,Ugai H,Moriyasu S,Minamihashi A,Yamamoto Y,Hirayama H,Kageyama S,Pan J,Murata T,Kobayashi Y,Obata Y,Yokoyama KK

    更新日期：2003-09-12 00:00:00