Evaluation of a rapamycin-regulated serotype 2 adeno-associated viral vector in macaque parotid glands.

Abstract:

OBJECTIVES:Salivary glands are useful target organs for local and systemic gene therapeutics. For such applications, the regulation of transgene expression is important. Previous studies by us in murine submandibular glands showed that a rapamycin transcriptional regulation system in a single serotype 2, adeno-associated viral (AAV2) vector was effective for this purpose. This study evaluated if such a vector was similarly useful in rhesus macaque parotid glands. METHODS:A recombinant AAV2 vector (AAV-TF-RhEpo-2.3w), encoding rhesus erythropoietin (RhEpo) and a rapamycin-inducible promoter, was constructed. The vector was administered to macaques at either of two doses [1.5 x 10(11) (low dose) or 1.5 x 10(12) (high dose) vector genomes] via cannulation of Stensen's duct. Animals were followed up for 12-14 weeks and treated at intervals with rapamycin (0.1 or 0.5 mg kg(-1)) to induce gene expression. Serum chemistry, hematology, and RhEpo levels were measured at interval. RESULTS:AAV-TF-RhEpo-2.3w administration led to low levels of rapamycin-inducible RhEpo expression in the serum of most macaques. In five animals, no significant changes were seen in serum chemistry and hematology values over the study. One macaque, however, developed pneumonia, became anemic and subsequently required euthanasia. After the onset of anemia, a single administration of rapamycin led to significant RhEpo production in this animal. CONCLUSION:Administration of AAV-TF-RhEpo-2.3w to macaque parotid glands was generally safe, but led only to low levels of serum RhEpo in healthy animals following rapamycin treatment.

journal_name

Oral Dis

journal_title

Oral diseases

authors

Zheng C,Voutetakis A,Metzger M,Afione S,Cotrim AP,Eckhaus MA,Rivera VM,Clackson T,Chiorini JA,Donahue RE,Dunbar CE,Baum BJ

doi

10.1111/j.1601-0825.2009.01631.x

subject

Has Abstract

pub_date

2010-04-01 00:00:00

pages

269-77

issue

3

eissn

1354-523X

issn

1601-0825

pii

ODI1631

journal_volume

16

pub_type

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