Abstract：
:Cultured cells derived from hamster insulinoma (In-111 R1 cells) were placed in 1.4 M dimethyl sulfoxide (Me2SO)-containing RPMI 1640 at 20 degrees C for 20 min. They were frozen to -40 degrees C at a cooling rate of 1.0 or 0.5 degrees C/min, subsequently to -80 degrees C at 3 degrees C/min with a programmable freezer. After being maintained at -80 degrees C, they were rapidly thawed to 37 degrees C. Thawed cells were washed with 0.75 M sucrose for removal of Me2SO. Recovered cells were cultured in 2 ml of RPMI 1640 with 1.3 mM theophylline under a gas phase of 95% air -5% CO2 at 37 degrees C for 2 days. In both cooling rates, frozen-thawed cells discharged more insulin than the thawed in the absence of theophylline. However, this released insulin level was higher in the cells frozen at a cooling rate of 0.5 degrees C/min than that at 1.0 degrees C/min. Moreover, insulin released from frozen-thawed hamster insulinoma cells increased significantly with the addition of 1.3 mM theophylline. Considering that the higher insulin release level at 11.1 mM glucose alone might indicate cellular damage, it is suggested that the cooling rate of 1 degree C/min may be better for cryopreservation of the dispersed cells under the present protocol for the assessment of the function of insulin release.
journal_name
Diabetes Res Clin Practjournal_title
Diabetes research and clinical practiceauthors
Ishihara K,Taniguchi H,Ejiri K,Baba Sdoi
10.1016/0168-8227(91)90097-wsubject
Has Abstractpub_date
1991-02-01 00:00:00pages
95-8issue
2eissn
0168-8227issn
1872-8227pii
0168-8227(91)90097-Wjournal_volume
11pub_type
杂志文章abstract：:The renal response to volume expansion (VE) has been shown to be impaired in streptozotocin (STZ)-induced diabetes. This may contribute to the abnormal maintenance of fluid balance in diabetics. Since calcium channel blockade (CaCb) has been shown to improve renal hemodynamic and tubular functions, the present studies...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/s0168-8227(97)01376-4
更新日期：1997-03-01 00:00:00
abstract：AIMS:To examine predictors of quality of life gains among people with type 1 diabetes following the Dose Adjustment for Normal Eating (DAFNE) self-management training programme. METHODS:Clinical and questionnaire data were collected from 437 patients from 6 hospital centres before, and 18 months post-DAFNE interventio...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2012.09.017
更新日期：2012-11-01 00:00:00
abstract：:The aim of this study was to investigate the effect of resveratrol on beta cells in male C57BL/6J mice fed a high-fat diet and the possible mechanisms. Male C57BL/6J mice were randomly divided into three groups: normal control (NC) group, high-fat diet (HF) group and high-fat diet and resveratrol treatment (HFR) group...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2012.02.029
更新日期：2012-09-01 00:00:00
abstract：AIMS:Type 2 diabetes (T2D) is a condition where the body becomes insulin resistant and cannot use insulin made by the pancreas or is relatively insulin deficient causing high blood glucose levels. Assessing the quality of clinical practice guidelines (CPGs) for T2D is important to identify knowledge gaps and where impr...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2020.108365
更新日期：2020-10-01 00:00:00
abstract：:We measured the cell-mediated immune response to GAD and bovine beta-casein in 38 type 1 and 37 type 2 diabetic patients who visited diabetic clinics or who were hospitalized in Bangkok, Thailand, and in 43 normal controls, by using a lymphoproliferation assay. Positive results against GAD were found in 29/38 (76.3%) ...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/s0168-8227(01)00322-9
更新日期：2002-03-01 00:00:00
abstract：AIMS:To determine if HLA-DP loci independently contribute to classic type 1 diabetes (T1D) of all ages, childhood-onset T1D and latent autoimmune diabetes in adults (LADA) among Chinese Han population. METHODS:A total of 518 patients with classic T1D (Among them 180 participants manifested T1D between 1-14 years), 519...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2020.108582
更新日期：2020-12-08 00:00:00
abstract：AIM:Dyslipidaemia may be a risk factor for diabetic kidney disease. We examined prospectively association between the use of statins and development of renal dysfunction in type 2 diabetes. METHODS:A consecutive cohort of 5264 diabetic patient recruited between 1996 and 2005 underwent detailed assessments. Renal dysfu...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2010.02.006
更新日期：2010-06-01 00:00:00
abstract：:The relationship between depressive symptoms and glycemic control in youth with type 2 diabetes was assessed at baseline (n = 682), 6, and/or 24 months (n = 576). Neither baseline nor persistence of depressive symptoms was significantly associated with maintenance of glycemic control. Nevertheless, depressive symptoms...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.diabres.2017.11.008
更新日期：2018-01-01 00:00:00
abstract：:Thirty diabetics who had been receiving ordinary insulin were switched to S-lente insulin, a new mixture of four parts semilente and six parts ultralente insulin. Eight times a day, we measured the glucose, insulin, and C-peptide (CPR) in their blood. Those with more than 250 mg/dl postprandial glucose were designated...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/0168-8227(90)90019-p
更新日期：1990-01-01 00:00:00
abstract：:Cause(s) of death in patients with diabetes mellitus (DM) admitted to a tertiary care hospital in North India was analysed from classified information in patients' death records and case file. Of the 306652 total admissions from 1991 to 1999, 21584 patients died (7.04%), 592 of whom (355 men and 237 women) had DM (2.7...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/s0168-8227(03)00013-5
更新日期：2003-05-01 00:00:00
abstract：BACKGROUND:The polymorphism 385 C/A of FAAH has been associated with overweight and obesity. The aim of our study was to investigate the relationship of polymorphism (cDNA 385 C-->A) of FAAH gene on obesity parameters in patients with diabetes mellitus type 2. DESIGN:A population of 70 patients with diabetes mellitus ...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2009.12.019
更新日期：2010-04-01 00:00:00
abstract：:A hypothesis that mitochondrial genome itself is the thrifty genome has been advanced, as both its mutation, or quality, and amount in the peripheral blood, or quantity, are found to have close relationship to diabetes mellitus, or more specifically insulin resistance. Mere presence of relationship, however, does not ...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,评审
doi：10.1016/s0168-8227(01)00336-9
更新日期：2001-12-01 00:00:00
abstract：:To investigate the cumulative incidence and mortality of COVID-19 and the prevalence of comorbidities such as diabetes, obesity and hypertension in regions with different altitude levels ranging from sea level to high altitude. METHODOLOGY: Analytical study in which we correlated secondary data obtained from reports o...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2020.108463
更新日期：2020-11-01 00:00:00
abstract：BACKGROUND:While the mechanisms leading to β-cell destruction and clinical onset of T1D are still unclear, the composition of the immune profile is probably important for the outcome of immune activity. The aim of this study was to investigate the composition and possible changes of the immunological profile, spontaneo...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2013.01.014
更新日期：2013-04-01 00:00:00
abstract：:The prevalence of obesity observed in this study seems higher than that reported in the previous study. This is probably due to the characteristics of the subjects studied, since they were subjects admitted for health examinations with possible selection. However, the observation in this study clearly indicated that o...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/0168-8227(90)90153-k
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVE:Several genome-wide association studies and replication analyses have identified common variation at the insulin-like binding protein 2 (IGF2BP2) gene to be associated with type 2 diabetes (T2DM). The aim of this study was to replicate in a Lebanese Arab population identified associations of IGF2BP2 variants ...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2011.12.026
更新日期：2012-05-01 00:00:00
abstract：OBJECTIVE:We examined the effect of a 14-month progressive resistance training (PRT) program on endothelial function in both a supervised training (Center) group and non-supervised training (Home) group of patients with type 2 diabetes. We studied 28 men and women with type 2 diabetes who participated in a 14-month PRT...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.diabres.2007.09.020
更新日期：2008-03-01 00:00:00
abstract：AIMS:We evaluated clinical outcome and risk factors affecting survival in patients with diabetes on continuous ambulatory peritoneal dialysis (CAPD) in Southern China. METHODS:This longitudinal cohort study enrolled all incident patients who used CAPD as their first renal replacement therapy modality in our center fro...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2013.03.030
更新日期：2013-06-01 00:00:00
abstract：OBJECTIVE:To examine change of diagnosis in patients from the German/Austrian multicenter DPV (Diabetes Patienten Verlaufsdokumentation) database initially classified as type 2 diabetes. METHODS:Patients aged ≤20 years at onset, diagnosed between 1995 and 2010 were followed for at least 6 months. Chi-square/Wilcoxon t...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,多中心研究
doi：10.1016/j.diabres.2011.09.011
更新日期：2011-12-01 00:00:00
abstract：:A 44-year-old woman with diabetes mellitus, cardiomyopathy, and a mitochondrial gene mutation, was reported. She was diagnosed as having diabetes at 33 years of age and was treated with insulin. However, she stopped treatment 6 months later and had no medical care until she developed diabetic ketoacidosis at 41 years ...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/0168-8227(95)01100-r
更新日期：1995-06-01 00:00:00
abstract：AIM:Bariatric surgery is associated with a significant improvement in glucose control and even diabetes remission. There are no studies investigating national trends in the use of bariatric surgery in people with type 2 diabetes. We examine trends in the use of bariatric surgery in patients with and without type 2 diab...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2012.12.011
更新日期：2013-03-01 00:00:00
abstract：AIMS:Central obesity, a prominent feature of metabolic syndrome (MetS), is commonly assessed by gender- and ethnicity-specific waist circumference (WC) cut-off values. Since 2006, the recommended WC cut-offs by the International Diabetes Federation (IDF) for Europeans are used in Iran because of limited data availabili...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2008.07.009
更新日期：2008-10-01 00:00:00
abstract：AIMS:To evaluate the Hypoglycaemia Symptom Rating Questionnaire (HypoSRQ©) and relationships between self-reported hypoglycaemia and hypoglycaemia measured using blinded continuous glucose monitoring (CGM). METHODS:Diabetes outpatients (n = 113) recruited from Ashford and St. Peter's Hospital completed the HypoSRQ (re...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2019.04.010
更新日期：2019-05-01 00:00:00
abstract：:Mutations of mitochondrial DNA have been found in cardiomyopathic and diabetic patients as well as mitochondrial myopathic patients. Mitochondrial DNA mutations are maternally transmitted. It has also been reported that these mutations can be acquired under the influence of free radicals. This article is a mini-review...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,评审
doi：10.1016/0168-8227(96)01239-9
更新日期：1996-07-01 00:00:00
abstract：AIM:To perform a network meta-analysis between long-acting insulin analogues (glargine and detemir) and Neutral Protamine Hagedorn (NPH) insulin on adults with type 1 diabetes. METHODS:A systematic review of the literature was conducted according to the Cochrane Collaboration guidelines. The search for randomized cont...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,评审
doi：10.1016/j.diabres.2011.09.001
更新日期：2011-12-01 00:00:00
abstract：AIMS:To evaluate the incidence of impaired glucose tolerance (IGT), impaired fasting glucose (IFG) and diabetes in 318 Caucasian women with gestational diabetes (GDM) at 6 weeks postpartum. METHODS:All women had 75g OGTT and the following data were collected: age, height, weight, results of the challenge 50g and diagn...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2009.04.003
更新日期：2009-06-01 00:00:00
abstract：:The effects of exercise on alterations in the amount of B-cell mass, insulin content and fibrous tissue present in the pancreas were examined for a diabetic state induced by a 70% pancreatectomy and a prediabetic state in Otsuka Long-Evans Tokushima Fatty (OLETF) rat, a model for the spontaneous development of non-ins...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/s0168-8227(96)01357-5
更新日期：1997-02-01 00:00:00
abstract：:Many studies show poor agreement between fasting plasma glucose (FPG)-based and 2-h postchallenge glucose (2-h PG)-based criteria to assess glucose metabolism. We examined the rate of agreement between FPG- and 2-h PG-based criteria in the diagnosis of intermediate hyperglycemia in four representative cohort studies i...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,多中心研究
doi：10.1016/j.diabres.2008.01.022
更新日期：2008-06-01 00:00:00
abstract：AIMS:Diabetic patients require care from a variety of health care providers, but little is known about their actual use of health care. Aims of this study were to (1) obtain information on health care use by diabetic patients in the Netherlands, (2) distinguish patterns of health care utilisation among these patients, ...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/s0168-8227(03)00116-5
更新日期：2003-09-01 00:00:00
abstract：:There is a growing body of evidence that the insulin-like growth factors (IGF-I and IGF-II) are dynamically involved in the regulation of glucose homeostasis, with one of their binding proteins, IGFBP-1, playing a counterregulatory role. The IGFs are structurally and functionally related to insulin and in the circulat...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,评审
doi：10.1016/0168-8227(94)90121-x
更新日期：1994-02-01 00:00:00
