Abstract：
:A number of cell-penetrating peptides (CPPs) have been reported, but their transduction efficiencies are too low to be used as intracellular carriers for therapeutic purposes. We conducted a comprehensive search to find novel CPPs using an in vitro virus (IVV) library, which presented random peptides consisting of 15 amino acids (diversity of the library was >10(12)). We found 9 kinds of novel CPPs with an intracellular translocation efficiency higher than that of the TAT peptide (YGRKKKRRQRRR). Interestingly, one of the novel CPPs, No. 14 (KLWMRWYSPTTRRYG), showed a dramatic improvement in translocation activity relative to the TAT peptide in CHO cells (>10-fold efficiency in 50 microM). As the intracellular translocation efficiency of No. 14 was increased by substitution Arg for Lys1 (14-1), we carried out alanine scanning on the basis of 14-1 to determine important amino acids for the intracellular translocation. The Ala substitution analysis showed that both Arg and Trp residues were important for the cell-penetrating activity and that their contribution was in the order Trp3 journal_name journal_title authors subject pub_date pages issue eissn issn journal_volume pub_type
abstract：:Despite recent advances in the management of thyroid cancer, the survival rate of this tumor may still be improved. Therefore, the identification of biological and molecular features of indolent and aggressive disease would be critical to define clinically useful predictors of high-...
journal_title：International journal of molecular medicine
pub_type： 杂志文章,评审
doi：10.3892/ijmm.2012.1089
更新日期：2012-11-01 00:00:00
abstract：:Wiskott-Aldrich syndrome (WAS) is an X-linked recessive disorder characterized by microthrombocytopenia, eczema, immunodeficiency, and susceptibility to lymphoid malignancy. Loss-of-function mutations in WAS gene have been identified to cause disorders with platelet defects including WAS and X-linked thrombocytopenia....
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
abstract：:The anti-cancer and cancer-preventive effects of green tea and its main constituent (-)-epigallocatechin gallate [(-)-EGCG] are well documented by a variety of studies, including epidemiological, cell culture, animal, and clinical studies. While (-)-EGCG remains the most potent polyphenol in green tea, it is very unst...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2005-04-01 00:00:00
abstract：:With the rapid economic development in recent years, China is facing a great challenge due to heavy metal pollution. The heavy metals may enter the human body through ingestion of aqua products to cause great health risks. In the present study, the inhibitory effects of luteolin on the combined toxicity of multi-heavy...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2017.3219
更新日期：2018-01-01 00:00:00
abstract：:p57 (Kip2) belongs to the Cip/Kip family and is one of the universal negative regulators of the cell cycle. In this study, we investigated the p57 expression of various types of thyroid neoplasm. p57 overexpression was observed in only 4.2% of normal thyroid tissues. In follicular adenoma and minimally invasive follic...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2002-04-01 00:00:00
abstract：:In recent studies, we and others have demonstrated that bone morphogenetic protein-2 (BMP-2) promotes vascularization, inhibits hypoxic cell death of cancer cells and may be involved in tumor angiogenesis. The activation of circulating endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) represents a ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.18.4.735
更新日期：2006-10-01 00:00:00
abstract：:The skin, the conjunctivae, the airways and the digestive tract compose a huge vulnerable biological surface, which is exposed to the external environment. An allergen can often trigger an allergic reaction at a number of sites or result in an atopic march. However, the mechanism of atopic march remains unclear. Less ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章,评审
doi：10.3892/ijmm_00000525
更新日期：2010-12-01 00:00:00
abstract：:The human immunodeficiency virus type 1 (HIV-1) epidemic in Brazil is spreading to small municipalities as well as the innermost parts of the country and scarce information has been reported on the frequency of HIV-1 resistance-associated mutations in these areas. To determine the frequency and diversity of the HIV-1 ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2010-10-01 00:00:00
abstract：:Cutaneous melanoma is very aggressive and results in high mortality rates for cancer patients. Determining molecular targets is important for developing novel therapies for cutaneous melanoma. Cell division cycle associated 8 (CDCA8) is a putative oncogene that is upregulated in multiple types of cancer. The present s...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2018.3985
更新日期：2019-01-01 00:00:00
abstract：:Telomerase activity and hTERT mRNA expression are upregulated in colorectal cancer. Whether they are inherent in colorectal adenomas, premalignant lesions to cancer, however, remains to be elucidated. We examined telomerase activity by the fluorescence-based telomeric repeat amplification protocol method and analyzed ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2002-08-01 00:00:00
abstract：:The number of patients suffering from diabetes mellitus is constantly rising worldwide, and diabetic retinopathy (DR) has become the most frequent cause of postnatal blindness. Vascular endothelial growth factor (VEGF) is known to play a central role during DR development. Thus, inhibiting the effects of VEGF may hamp...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:POU domain proteins are a family of critical regulators of development and differentiation due to their transcriptional activity in the nucleus. Skn‑1a, a member of the POU domain protein family, appears to be expressed predominantly in epidermal keratinocytes and is thought to play a critical role in keratinocyte dif...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2014.1803
更新日期：2014-08-01 00:00:00
abstract：:ACSL3 is a member of the long chain acyl-CoA synthetase (ACSL) family that consists of 5 isozymes responsible for cellular fatty acid metabolism in various tissues in an isozyme-specific manner. Our previous studies have demonstrated that expression of ACSL3 mRNA and protein in liver was specifically increased after f...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2011.624
更新日期：2011-05-01 00:00:00
abstract：:Ouabain, an inhibitor of the Na+/K+-ATPase, has been reported to affect the secretory activity of the adrenal cortex, and especially of zona glomerulosa (ZG). However, conflicting results were obtained, depending on the experimental condition used since ouabain appears to interact with angiotensin-II (Ang-II) and its ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2006-08-01 00:00:00
abstract：:Periplaneta americana extracts (PAEs) play a crucial role in skin wound healing. However, their molecular effects and signaling pathways in regenerating tissues and cells are not clear. In this study, we refined the PAE from Periplaneta americana to investigate the mechanisms underlying skin wound healing. The human k...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2017.3040
更新日期：2017-08-01 00:00:00
abstract：:Induction of apoptosis is one of the mechanisms of chemotherapeutic agents against breast cancer. In addition, recent studies have shown that diets containing polyphenolic components possess anticancer activities either in vitro or in vivo by inhibiting cell proliferation and inducing apoptosis. The aim of our study w...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2010-07-01 00:00:00
abstract：:Laminarin, found in marine brown algae, is used as a carbohydrate reserve for phytoplankton; however, it is also used in traditional Chinese medicine, and has been shown to have several biological activities, including anticancer activities. In this study, we examined the mechanisms through which laminarin from Lamina...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2013.1409
更新日期：2013-08-01 00:00:00
abstract：:We analyzed a series of ovarian carcinomas from patients treated with cisplatin-based chemotherapy for loss of heterozygosity (LOH) to determine the relationship between microsatellite alteration and prognosis. Patients with tumors that had lost alleles at loci 3p14-21, 12qter, or 17p13.3 showed significantly reduced ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：:Although diabetes mellitus has been recognized as a risk factor for chronic kidney disease (CKD), genetic factors for predisposition to CKD in individuals with diabetes mellitus remain elucidated. The purpose of the present study was to identify genetic variants that confer susceptibility to CKD among individuals with...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm_00000161
更新日期：2009-04-01 00:00:00
abstract：:Soy sauce (Shoyu) is a traditional Japanese fermented seasoning and is available worldwide. We investigated the effect of Shoyu polysaccharides (SPS) prepared from soy sauce on the intestinal immune system of mice. SPS enhanced the production of immunoglobulin A (IgA) from Peyer's patch cells in vitro, and its oral ad...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2008-08-01 00:00:00
abstract：:Blood supply plays a crucial role in solid tumour development and leukaemogenesis. It has been suggested that blocking of angiogenesis could be possible in cancer therapy. We have demonstrated the antiproliferative activity of Gleditsia sinensis fruit extract (GSE) on various human solid tumour cancer cell lines as we...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2003-08-01 00:00:00
abstract：:Liver ischemia represents a common clinical problem. In the present study, using an in vitro model of hepatic ischemia-reperfusion injury, we evaluated the potential cytoprotective effect of the purine metabolites, such as adenosine and inosine, and studied the mode of their pharmacological actions. The human hepatoce...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2012.1203
更新日期：2013-02-01 00:00:00
abstract：:Icariin (ICA) and icaritin (ICT), with a similar structure to genistein, are the important bioactive components of the genus Epimedium, and regulate many cellular processes. In the present study, using the estrogen receptor (ER)-negative breast cancer cell line, SKBr3, as a model, we examined the hypothesis that ICA a...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2014.1722
更新日期：2014-06-01 00:00:00
abstract：:Heparin/heparan sulfate interacting protein (HIP) was initially identified as an adhesion molecule from a human uterine epithelial cell line. It was previously demonstrated that HIP was upregulated in human colorectal cancer. However, its expression was significantly lower in Dukes' D samples compared to earlier Dukes...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2003-04-01 00:00:00
abstract：:Bladder cancer (BC) is one of the most common malignant tumors in males globally. Its progression imposes a heavy burden on patients; however, the expression profile of circular (circ)RNAs in BC progression remains unclear. This study explored changes in circRNA expression during BC progression by sequencing different...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2019.4340
更新日期：2019-11-01 00:00:00
abstract：:The present study aimed to explore the underlying mechanisms of bone morphogenetic protein 2 (BMP2) in alleviating intervertebral disc degeneration (IDD). A rat puncture IDD model was constructed, and the rats were randomly divided into six groups: Control; IDD (model); IDD+PBS [containing 1010 adeno‑associated virus ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2018.3972
更新日期：2019-01-01 00:00:00
abstract：:Single-prolonged stress (SPS) is an established animal model for post-traumatic stress disorder (PTSD). The calcium (Ca2+)-calmodulin (CaM)-CaM kinase (K)IIalpha signal passage plays an important role in the plasticity of central nervous, learning and memory, mind and behavior and other types of cognitive activities. ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm_00000294
更新日期：2009-12-01 00:00:00
abstract：:The response of living cells to change in cell environment depends on the action of second messenger molecules. The two second messenger molecules cAMP and Ca2+ regulate a large number of eukaryotic cellular events. Calmodulin-stimulated cyclic nucleotide phosphodiesterase (PDE1) is one of the key enzymes involved in ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章,评审
doi：
更新日期：2006-07-01 00:00:00
abstract：:The prognosis for patients with metastatic bladder cancer (BCa) is poor, and has not been improved by current treatment methods. Long noncoding RNAs (lncRNAs) are involved in the pathology of various tumors, including bladder cancer. However, the role of zinc finger E‑box‑binding homeobox 1‑antisense 1 (ZEB1‑AS1) in B...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2019.4187
更新日期：2019-07-01 00:00:00
abstract：:Post-transcriptional control of mRNA trafficking and metabolism plays a critical role in the actualization and fine tuning of the genetic program of cells, both in development and in differentiated tissues. Cis-acting signals, responsible for post-transcriptional regulation, reside in the RNA message itself, usually i...
journal_title：International journal of molecular medicine
pub_type： 杂志文章,评审
doi：10.3892/ijmm.2014.1629
更新日期：2014-04-01 00:00:00
