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Rapid isolation of novel FK506 binding proteins from multiple organisms using gDNA and cDNA T7 phage display.

Abstract：

:Reverse chemical proteomics using T7 phage display is a powerful technique for identifying cellular receptors of biologically active small molecules. However, to date this method has generally been limited to cDNA libraries constructed from mRNA isolated from eukaryotes. In this paper, we describe the construction of the first prokaryotic T7 phage display libraries from randomly digested Pseudomonas stutzeri and Vibrio fischeri gDNA, as well as a plant cDNA library from Arabidopsis thaliana. We also describe the use of T7 phage display to identify novel proteins from environmental DNA samples using biotinylated FK506 as a model affinity probe.

journal_name

Bioorg Med Chem

journal_title

Bioorganic & medicinal chemistry

authors

Piggott AM,Kriegel AM,Willows RD,Karuso P

doi

10.1016/j.bmc.2009.08.039

subject

Has Abstract

pub_date

2009-10-01 00:00:00

pages

6841-50

issue

19

eissn

0968-0896

issn

1464-3391

pii

S0968-0896(09)00798-6

journal_volume

17

pub_type

杂志文章

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