Metallopeptidase inhibitors arrest vital biological processes in the fungal pathogen Scedosporium apiospermum.

Abstract:

:Scedosporium apiospermum is an emerging agent of opportunistic mycoses in humans. Previously, we showed that mycelia of S. apiospermum secreted metallopeptidases which were directly linked to the destruction of key host proteins. In this study, we analysed the effect of metallopeptidase inhibitors on S. apiospermum development. As germination of inhaled conidia is a crucial event in the infectious process of S. apiospermum, we studied the morphological transformation induced by the incubation of conidia in Sabouraud-dextrose medium at 37 °C. After 6 h, some conidia presented a small projection resembling a germ-tube. A significant increase, around sixfold, in the germ-tube length was found after 12 h, and hyphae were exclusively observed after 24 h. Three distinct metallopeptidase inhibitors were able to arrest the transformation of conidia into hyphae in different ways; for instance, 1,10-phenanthroline (PHEN) completely blocked this process at 10 μmol l(-1), while ethylenediamine tetraacetic acid (EDTA) and ethylene glycol-bis (β-aminoethyl ether; EGTA) only partially inhibited the differentiation at up to 10 mmol l(-1). EGTA did not promote any significant reduction in the conidial growth, while PHEN and EDTA, both at 10 mmol l(-1), inhibited the proliferation around 100% and 65%, respectively. The secretion of polypeptides into the extracellular environment and the metallopeptidase activity secreted by mycelia were completely inhibited by PHEN. These findings suggest that metallo-type enzymes could be potential targets for future therapeutic interventions against S. apiospermum.

journal_name

Mycoses

journal_title

Mycoses

authors

Silva BA,Souza-Gonçalves AL,Pinto MR,Barreto-Bergter E,Santos AL

doi

10.1111/j.1439-0507.2009.01767.x

subject

Has Abstract

pub_date

2011-03-01 00:00:00

pages

105-12

issue

2

eissn

0933-7407

issn

1439-0507

pii

MYC1767

journal_volume

54

pub_type

杂志文章

相关文献

MYCOSES文献大全
  • Griseofulvin vs terbinafine for paediatric tinea capitis: When and for how long.

    abstract::Outbreaks of tinea capitis (TC) can be a source of medical and economic burden for healthcare systems; Griseofulvin and terbinafine are considered to be effective first-line treatments for TC. In order to compare the efficacy of griseofulvin and terbinafine for the treatment of TC in a paediatric population of African...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12970

    authors: Bar J,Samuelov L,Sprecher E,Mashiah J

    更新日期:2019-10-01 00:00:00

  • Dystrophic onychomycosis due to Microsporum gypseum.

    abstract::A case of onychomycosis with nail dystrophy due to Microsporum gypseum in a healthy 35-year-old woman is described. The infection had a 2-year history, occurring in the site of a nail trauma sustained in a fall from a horse. Diagnosis was based on mycological examination (direct microscope observation and culture). Cl...

    journal_title:Mycoses

    pub_type: 信件

    doi:10.1111/j.1439-0507.2006.01248.x

    authors: Romano C,Ghilardi A,Fimiani M

    更新日期:2006-07-01 00:00:00

  • Case report. Rosacea-like Tinea incognito.

    abstract::We report a case of dermatophytosis of the face due to Microsporum canis that was exacerbated and altered clinically by a long-term application of topical corticosteroids. We considered this case a rosacea-like tinea incognito of the beard area. ...

    journal_title:Mycoses

    pub_type: 信件

    doi:10.1046/j.1439-0507.2002.00742.x

    authors: Gorani A,Schiera A,Oriani A

    更新日期:2002-04-01 00:00:00

  • Adherence of Candida albicans and Candida parapsilosis to epithelial cells correlates with fungal cell surface carbohydrates.

    abstract::Many studies have described the adherence of Candida albicans to epithelial cells but little is known about Candida parapsilosis adhesion and its role in host cell surface recognition. This study was designed to evaluate the correlation between the adherence of 20 C. albicans and 12 C. parapsilosis strains to human bu...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2009.01757.x

    authors: Lima-Neto RG,Beltrão EI,Oliveira PC,Neves RP

    更新日期:2011-01-01 00:00:00

  • [Prophylaxis and therapy of fungal infections with fluconazole in patients after bone marrow transplantation].

    abstract::In an open study 31 patients undergoing bone marrow transplantation for various haematological diseases received fluconazole as prophylaxis or treatment of fungal infections. In 26 of these patients an antecedent oral prophylaxis with polyene antimycotics had failed to prevent infections with Candida species. Five of ...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:

    authors: Quabeck K,Müller KD,Beelen DW,Dermoumi H,Kölbel M,Kraft J,Schaefer UW

    更新日期:1990-01-01 00:00:00

  • Severe fungal sclerokeratitis caused by Metarhizium anisopliae: a case report and literature review.

    abstract::To date, there has been only one published report on the infectious sclerokeratitis caused by Metarhizium anisopliae, which is an entomopathogenic fungus. Regarding corneal infection, three reports have been published to date. Although the prognoses of the corneal infections are favourable, prognosis when scleral infe...

    journal_title:Mycoses

    pub_type: 杂志文章,评审

    doi:10.1111/myc.12279

    authors: Eguchi H,Toibana T,Hotta F,Miyamoto T,Mitamura Y,Yaguchi T

    更新日期:2015-02-01 00:00:00

  • Turbidometric characterization of the postantifungal effect: comparative studies with amphotericin B, 5-fluorocytosine and miconazole on Candida albicans.

    abstract::The phenomenon of persistent suppression of Candida albicans yeast cell growth after short drug exposures (postantifungal effect, PAFE) was determined by performing comparative studies using different concentrations of 5-fluorocytosine (5-FC), amphotericin B (AMPH) and miconazole (MCZ). An in vitro turbidometric metho...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1991.tb00663.x

    authors: Scalarone GM,Mikami Y,Kurita N,Ichihara Y,Yazawa K,Miyaji M

    更新日期:1991-07-01 00:00:00

  • Geographical evaluation of Neuroparacoccidioidomycosis and Paracoccidioidomycosis in Southern Brazil.

    abstract::Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis among immunocompetent patients in Latin America. This study aimed to describe the expansion over time and the geographical distribution of confirmed Neuroparacoccidioidomycosis (NPCM) and PCM cases, and relate it to environmental characteristics such ...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12782

    authors: de Almeida SM,Salvador GLO,Roza TH,Izycki LF,Dos Santos I,Aragão A,Kulik A,Muro M,Torres LFB,de Noronha L

    更新日期:2018-08-01 00:00:00

  • Pityriacitrin--a potent UV filter produced by Malassezia furfur and its effect on human skin microflora.

    abstract::In Malassezia furfur, tryptophan as the main nitrogen source induces production of the potent ultraviolet-absorbing indole compound pityriacitrin. An in vitro study about the effects of pityriacitrin on other human skin microorganisms is presented, with special focus on Candida albicans and staphylococci in which its ...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2006.01265.x

    authors: Machowinski A,Krämer HJ,Hort W,Mayser P

    更新日期:2006-09-01 00:00:00

  • Genotypic relatedness of yeast isolates from women infected with human immunodeficiency virus and their children.

    abstract::The objective of this study was to compare polymerase chain reaction (PCR) fingerprinting with other molecular typing methods as an epidemiologic tool to investigate the transmission of Candida strains between HIV-positive mothers and their children. Forty-nine yeast strains (including Candida albicans, Candida glabra...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1046/j.1439-0507.1999.00479.x

    authors: Ruhnke M,Grosch-Wörner I,Lischewski A,Neubauer A,Steinmüller A,Trautmann M,Morschhäuser J

    更新日期:1999-01-01 00:00:00

  • Screening for asymptomatic scalp carriage in household contacts of patients with tinea capitis during 1997-2011: a retrospective hospital-based study.

    abstract::For anthropophilic tinea capitis (TC), household spread and asymptomatic scalp carriage (ASC) is considered an important route of transmission and incomplete clearance. To investigate ASC in household contacts of patients diagnosed with TC in a tertiary hospital in Athens, Greece, we retrospectively reviewed the medic...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12166

    authors: Dessinioti C,Papadogeorgaki E,Athanasopoulou V,Antoniou C,Stratigos AJ

    更新日期:2014-06-01 00:00:00

  • Confocal laser scanning microscopy as a new valuable tool in the diagnosis of onychomycosis - comparison of six diagnostic methods.

    abstract::Onychomycosis is common and can mimic several different nail disorders. Accurate diagnosis is essential to choose the optimum antifungal therapy. The aim of this study was to evaluate the use of confocal laser scanning microscopy (CLSM) and optical coherence tomography (OCT) as new non-invasive diagnostic tools in ony...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2012.02198.x

    authors: Rothmund G,Sattler EC,Kaestle R,Fischer C,Haas CJ,Starz H,Welzel J

    更新日期:2013-01-01 00:00:00

  • Our recommendations for avoiding exposure to fungi outside the hospital for patients with haematological cancers.

    abstract::Despite several chemotherapeutic and preventative advances, opportunistic fungal infections remain common unintended consequences of cancer treatment. Currently, cancer patients spend most of their time between treatments at home, where they can inadvertently come across potential hazards from environmental and food s...

    journal_title:Mycoses

    pub_type: 杂志文章,评审

    doi:10.1111/myc.12167

    authors: Ariza-Heredia EJ,Kontoyiannis DP

    更新日期:2014-06-01 00:00:00

  • Comparative stability, sensitivity and specificity studies with different lots of Blastomyces dermatitidis yeast and mycelial lysate antigens.

    abstract::Comparative studies were performed to assess the stability and lot-to-lot variation of Blastomyces dermatitidis yeast and mycelial phase lysate antigens. Four lots were prepared from each growth phase of B. dermatitidis strain T-58 (canine isolate) during a 14-month period. Serum specimens from dogs with blastomycosis...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1994.tb00293.x

    authors: Orr ML,Bono JL,Abuodeh RO,Williams SJ,Legendre AM,Scalarone GM

    更新日期:1994-06-01 00:00:00

  • Frequency, type and treatment of fungal pathogens in toenail onychomycosis in the central Alpine region of South Tyrol, northern Italy - a 10-year retrospective study from 2004 to 2013.

    abstract::Onychomycosis is one of the most common dermatological diseases, primarily caused by dermatophytes, and sometimes, also by yeast and moulds. This study aims to clarify the frequency of isolated pathogens from mycotic toenails in the reference centre of the central Alpine mountain region of South Tyrol, northern Italy....

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12528

    authors: Gasser J,Pagani E,Vittadello F,Nobile C,Zampieri P,Eisendle K

    更新日期:2016-12-01 00:00:00

  • Serum antibody levels correlate with oral fungal cell numbers and influence the patients' response to chronic paracoccidioidomycosis.

    abstract::Paracoccidioidomycosis (PCM) is a neglected fungal disease that elicits an important granulomatous inflammatory reaction which aims to isolate the fungi and resolve the infection; besides the innate cellular response, the patients' sera may contain different levels of antibodies directed against PCM's pathogenic agent...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12325

    authors: de Carli ML,Cardoso BC,Malaquias LC,Nonogaki S,Pereira AA,Sperandio FF,Hanemann JA

    更新日期:2015-06-01 00:00:00

  • Study of the ITS region in an atypical isolate and comparison with six species of Microsporum.

    abstract::Microsporum species are a frequent cause of cutaneous mycoses in humans. Atypical strains of Microsporum can sometimes be difficult to identify with conventional methods. Recently, we have obtained a Microsporum isolate with atypical morphology and special nutritional requirements (Microsporum CHUS-126-02). As several...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2006.01281.x

    authors: Leon-Mateos A,Paredes-Suárez C,Pereiro M Jr,Toribio J

    更新日期:2006-11-01 00:00:00

  • First application of loop-mediated isothermal amplification (LAMP) assays for rapid identification of mating type in the heterothallic fungus Aspergillus fumigatus.

    abstract:BACKGROUND:Loop-mediated isothermal amplification (LAMP) assays, which operate at a single temperature and require no postreaction processing, have been described for rapid species-specific detection of numerous fungi. The technology has much less commonly been applied to identification of other key genetic traits such...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12959

    authors: King KM,Hawkins NJ,Atkins S,Dyer PS,West JS,Fraaije BA

    更新日期:2019-09-01 00:00:00

  • Unilateral renal phaeohyphomycosis due to Bipolaris spicifera in an immunocompetent child - rare case presentation and review of literature.

    abstract::Phaeohyphomycosis refers to infections caused by phaeoid fungi that can have an aggressive course in normal hosts. We report a case of left-sided renal phaeohyphomycosis due to Bipolaris spicifera in a 7-year-old immunocompetent male child. He presented with fever, dysuria, nausea, vomiting and flank pain. Examination...

    journal_title:Mycoses

    pub_type: 杂志文章,评审

    doi:10.1111/myc.12335

    authors: Kaur R,Bala K

    更新日期:2015-07-01 00:00:00

  • Non-response to fluconazole maintenance treatment (ReCiDiF regimen) for recurrent vulvovaginal candidosis is not related to impaired glucose metabolism.

    abstract::Is non-response to maintenance treatment for recurrent vulvovaginal candidosis (RCVV) related to the impaired glucose metabolism? In the ReCiDiF trial, women with RCVV were given a degressive regimen with fluconazole according to their clinical, microscopic and mycologic response. Data obtained from optimal, suboptima...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12626

    authors: Grinceviciene S,Bellen G,Ruban K,Donders G

    更新日期:2017-08-01 00:00:00

  • Podopompholyx due to pemphigus vulgaris and Trichophyton rubrum infection. Report of an unusual case.

    abstract::We report the case of a 48-year old man who after a disease-free interval of 7 years developed a relapse of pemphigus vulgaris (PV) presenting as pompholyx of the left foot. The patient was first thought to suffer from a dyshidrosic form of tinea pedis due to Trichophyton rubrum. Although the concomitant occurrence of...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1994.tb00790.x

    authors: Borradori L,Harms M

    更新日期:1994-03-01 00:00:00

  • Evaluation of different commercial ELISA methods for the serodiagnosis of systemic candidosis.

    abstract::Different commercial enzyme-linked immunsorbent assays (ELISA) were evaluated in a preliminary study for diagnosis of systemic candidosis: Biomerica and Virotech GmbH, which allowed immunoglobulin G detection, and Platelia, which associated total antibody to antigen detection. They were tested with a home-made ELISA a...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1046/j.1439-0507.2002.00775.x

    authors: Persat F,Topenot R,Piens MA,Thiebaut A,Dannaoui E,Picot S

    更新日期:2002-12-01 00:00:00

  • Ultrastructural investigations of Candida albicans after in vitro treatment with the new fungicidal agent rilopirox.

    abstract::Candida albicans was maintained in various culture media and incubated with different concentrations of the antifungal agent rilopirox. After fixation, dehydration and embedding in Spurr's medium, the cells were analysed at the ultrastructural level to investigate morphological aspects of the antifungal mode of action...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1993.tb00727.x

    authors: Reitze HK,Dannhorn DR,Hänel H,Seitz KA

    更新日期:1993-11-01 00:00:00

  • [Molecular biological identification of Cuninghamella spec].

    abstract::Among the genus Cunninghamella, so far C. bertholletiae is known to be the only clinically relevant species. Correct identification of C. bertholletiae is not possible with classical methods. PCR and sequencing of the internal transcribed spacer (ITS) region was used to identify seven of nine clinical isolates as C. b...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2002.tb04543.x

    authors: Lemmer K,Losert H,Rickerts V,Just-Nübling G,Sander A,Kerkmann ML,Tintelnot K

    更新日期:2002-01-01 00:00:00

  • Pseudomembranous-like tinea of the scrotum: report of six cases.

    abstract::Six cases of tinea of the scrotum with atypical clinical features were observed in the dermatology department of our hospital between 2001 and 2007. The age of onset ranged from 14 to 26 years. Unusual clinical presentations of pseudomembranous-like disease were observed in every patient. Causative agents were Microsp...

    journal_title:Mycoses

    pub_type: 信件

    doi:10.1111/j.1439-0507.2008.01557.x

    authors: Lu S,Xi LY,Zhang JM,Lu CM

    更新日期:2009-05-01 00:00:00

  • Eosinophilic fungal rhinosinusitis due to the unusual pathogen Curvularia inaequalis.

    abstract::Among fungi, Curvularia inaequalis is a rare pathogen. We report the first case of non-invasive fungal rhinosinusitis caused by this species. Endoscopic sinus surgery revealed massive polyposis and the presence of viscous eosinophilic mucus that allowed the growth of the fungus. We diagnosed eosinophilic fungal rhinos...

    journal_title:Mycoses

    pub_type: 信件

    doi:10.1111/j.1439-0507.2008.01671.x

    authors: Posteraro B,Scarano E,La Sorda M,Torelli R,De Corso E,Mulé A,Paludetti G,Fadda G,Sanguinetti M

    更新日期:2010-01-01 00:00:00

  • Invasive metastasing pulmonary aspergillosis under polychemotherapy.

    abstract::We report on a female patient who received a polychemotherapy because of an extensive pemphigus vulgaris. Under this therapy, the general condition of the patient worsened. Noncharacteristic symptoms, such as dyspnoea and tachycardia occurred. In spite of comprehensive, also invasive diagnostics, pulmonary foci demons...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1994.tb00393.x

    authors: Sima D,Tietz HJ,Reisshauer H,Buchholtz I,Schulze P,Meyer R

    更新日期:1994-11-01 00:00:00

  • Production of Trichophyton rubrum microspores in large quantities and its application to evaluate amorolfine/azole compound interactions in vitro.

    abstract::Trichophyton rubrum is the most frequently isolated dermatophyte species in European countries. The lack or poor sporulation of T. rubrum has always been a major complication and a limiting factor when performing antifungal susceptibility testing. Therefore, we describe an in vitro method aiming to enhance sporulation...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12632

    authors: Laurent A,Monod M

    更新日期:2017-09-01 00:00:00

  • Cryptococcosis in cirrhotic patients.

    abstract::The objective of the present study was to report on three distinct forms of presentation of Cryptococcus neoformans infection in three cirrhotic patients. One patient had disseminated cryptococcosis with detection of the fungus in ascitic fluid, cerebrospinal fluid and blood; the second patient had pleural involvement...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2004.01064.x

    authors: França AV,Carneiro M,dal Sasso K,Souza Cda S,Martinelli A

    更新日期:2005-01-01 00:00:00

  • Molecular diagnostics of clinical strains of filamentous Basidiomycetes.

    abstract::Eleven clinical and veterinary strains of filamentous Basidiomycetes were compared with 15 reference strains representing the orders Aphyllophorales and Agaricales. The methods used were restriction analysis of small subunit (18S) (SSU) rDNA and internal transcribed spacers (ITS) 1 and 2 and variable domain 9 (V9)-ITS...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1998.tb00321.x

    authors: de Hoog GS,Gerrits van den Ende AH

    更新日期:1998-05-01 00:00:00