Abstract:
BACKGROUND:Loop-mediated isothermal amplification (LAMP) assays, which operate at a single temperature and require no postreaction processing, have been described for rapid species-specific detection of numerous fungi. The technology has much less commonly been applied to identification of other key genetic traits such as fungicide resistance, and has not yet been applied to mating-type determination in any fungus. OBJECTIVES:To develop first LAMP assays for mating-type identification in a fungus, in this instance with the saprophytic mould and human opportunistic pathogen Aspergillus fumigatus, a heterothallic ascomycete requiring isolates of opposite mating type (MAT1-1, MAT1-2) for sexual reproduction. METHODS:New LAMP primer sets, targeted to MAT gene sequences, were screened against 34 A fumigatus isolates (of known mating type) from diverse clinical, environmental and geographic sources to establish whether they could distinguish MAT1-1 or MAT1-2 genotypes. RESULTS AND CONCLUSIONS:The new assays, operating at a single temperature of 65°C, correctly identified the mating type of A fumigatus isolates in <20 minutes, and thus have numerous research and practical applications. Similar MAT LAMP assays could now be developed for other fungi of agricultural, environmental, industrial and/or medical importance.
journal_name
Mycosesjournal_title
Mycosesauthors
King KM,Hawkins NJ,Atkins S,Dyer PS,West JS,Fraaije BAdoi
10.1111/myc.12959subject
Has Abstractpub_date
2019-09-01 00:00:00pages
812-817issue
9eissn
0933-7407issn
1439-0507journal_volume
62pub_type
杂志文章相关文献
MYCOSES文献大全abstract::An atypical strain of Microsporum canis was isolated in our laboratory, which was characterized by a variety of polymorphous macroconidia. These are demonstrated by illustrations. Based on our observation and literature, the relationship between Microsporum canis and Microsporum distortum is discussed. Epidemiologic a...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1989.tb02167.x
更新日期:1989-01-01 00:00:00
abstract::Candida albicans, the most important opportunistic fungal pathogen, is able to generate remarkable amounts of reactive oxygen species (ROS). Since ROS are highly cytotoxic, this mechanism may contribute to the pathogenicity of this yeast, including its invasiveness and the inflammatory response of the host. Terbinafin...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1046/j.1439-0507.2002.00756.x
更新日期:2002-06-01 00:00:00
abstract::The localisation of a dermatophytosis on the face is rare. Differential diagnoses include a broad range of dermatological disorders, e. g. contact dermatitis, psoriasis vulgaris, seborrhoeic dermatitis, demodicosis, and polymorphic photo eruptions. Two patients suffering from tinea faciei incognito caused by Trichophy...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2007.01426.x
更新日期:2007-01-01 00:00:00
abstract::Pathogenicity of fungi is connected with their ability to easily penetrate the host tissues, survive in the infected host organism and use the elements of the host tissues as nutrients. Hence, the co-occurrence of pathogenic properties with the high enzymatic activity, which is manifested through the production of var...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2009.01696.x
更新日期:2010-05-01 00:00:00
abstract::The infection of the capillitium of an 8-year old child due to Microsporum canis is described. The child occasionally visited its mother working at an animal farm. The focus of the infection was found in farm rabbits infected by M. canis. A contamination of the environment (cages and floors) by the above mentioned der...
journal_title:Mycoses
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::The autopsy rates in Germany became low like in other European, American and Asian countries. Main reasons for this development are the lack of acceptance of autopsy in the society as well as in the medical profession, the introduction of a requirement for consent, unclear legal position, the public health system, pre...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0507.2007.01486.x
更新日期:2008-07-01 00:00:00
abstract::Scedosporium prolificans infections of normal hosts usually require extensive debridement and sometimes amputation to effect cure, due to the intrinsic resistance of this species to available antifungal agents. Newer agents have not tested favourably. Variable results are obtained with voriconazole, and 100% resistanc...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1046/j.1439-0507.2003.00878.x
更新日期:2003-06-01 00:00:00
abstract::This report analyses the frequency, the pattern and the severity of mycotic infection in a necropsy series of 103 bone marrow transplantations. Fungal infection was documented in 33 (32%) of all death cases. The risk of mycotic infection was increased in patients with graft failure and after retransplantation. At post...
journal_title:Mycoses
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::A survey of superficial skin mycoses was carried out among miners and office workers employed in different establishments in Jos, Nigeria. Mycotic infection was demonstrable by microscopy and culture in 45 (10.4%) subjects: 20 males and 25 females. Malassezia furfur was the predominant aetiological agent, followed by ...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1995.tb00058.x
更新日期:1995-05-01 00:00:00
abstract::The effect of four media or media combinations on the growth and the in vitro susceptibility to amphotericin B, 5-fluorocytosine, fluconazole and itraconazole of 51 isolates of Cr. neoformans was studied. The drug concentration producing a 50% decrease in OD405 (IC50) was determined in microtiter format. The following...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1996.tb00125.x
更新日期:1996-05-01 00:00:00
abstract::Two Candida albicans isolates were collected from a HIV-positive patient with recurrent oropharyngeal candidosis (OPC). One isolate was taken during the first episode of oral candidosis [fluconazole susceptible (FLU-S), minimal inhibitory concentration (MIC) = 0.25 mg l(-1) ] and the second after the patient developed...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2010.01970.x
更新日期:2011-09-01 00:00:00
abstract::We report a case of dermatophytosis of the face due to Microsporum canis that was exacerbated and altered clinically by a long-term application of topical corticosteroids. We considered this case a rosacea-like tinea incognito of the beard area. ...
journal_title:Mycoses
pub_type: 信件
doi:10.1046/j.1439-0507.2002.00742.x
更新日期:2002-04-01 00:00:00
abstract::Systematic studies about pet guinea pigs with dermatophytosis are rare. The aim of this study was to evaluate clinical signs, therapy and zoonotic risk of pet guinea pigs with dermatophytosis. Questionnaires from both owners (n = 74) of pet guinea pigs with dermatophytosis and their veterinarians (n = 101) were analys...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2012.02228.x
更新日期:2013-03-01 00:00:00
abstract::The serological responses to Cryptococcus neoformans proteins of blood donors and HIV patients with active cryptococcosis from a tropical region were investigated in this study. Exposure to C. neoformans, an organism ubiquitous in the environment, contributes to the antibody responses observed in the blood donors. IgG...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2008.01549.x
更新日期:2009-03-01 00:00:00
abstract:BACKGROUND:Pulmonary cryptococcosis (PC) is not considered an rare, opportunistic infection anymore. The immunocompetent population accounts for an increasing proportion of the morbidity. OBJECTIVE:This study investigated the clinical characteristics of PC patients spanning 20 years, in a referral centre of China. PA...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.12966
更新日期:2019-10-01 00:00:00
abstract::Non-steroidal anti-inflammatory drugs (NSAIDs) are one of the most common pharmacological agents. They have three primary therapeutic properties including anti-inflammatory, anti-pyretic and analgesic effects. Seven NSAIDs were tested against two species of dermatophytes. Percentage inhibition was determined for effec...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2010.01945.x
更新日期:2011-09-01 00:00:00
abstract::The authors report a case of subcutaneous mucormycosis in a diabetic patient with verrucous lesions in the fourth finger of the right hand. Initially diagnosed as a fixed cutaneous sporotrichosis case, success was obtained with the use of potassium iodide with healing of the lesion in a period of nearly 5 months. At p...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.1990.33.5.241
更新日期:1990-05-01 00:00:00
abstract::Studies of the genetic diversity of Candida albicans strains and the correlation between the antifungal susceptibility and gene diversity of C. albicans were carried out and the results were found to be inconsistent. To investigate antifungal susceptibility and genotypes of C. albicans strains from patients with vulvo...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2008.01539.x
更新日期:2009-01-01 00:00:00
abstract::Malassezia species are implicated in the pathogenesis of seborrhoeic dermatitis (SD), but the relationship between each species and the disorder remains unclear. It is hypothesised that the pathogenesis of SD has an immune component, which is supported by the increased incidence in patients with immunosuppressive diso...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2009.01713.x
更新日期:2010-07-01 00:00:00
abstract::A universal PCR-assay for the detection of fungal DNA was compared with microscopy and culture for the diagnosis of invasive aspergillosis using 78 samples from 42 patients. Eighteen patients were suffering from invasive aspergillosis, 5 patients were colonized with Aspergillus in the respiratory tract, 19 patients di...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1998.tb00605.x
更新日期:1998-01-01 00:00:00
abstract::A 45-year-old woman was found to have cerebral phaeohyphomycosis caused by an Exophiala species. The portal of entry of the fungus remained unknown. Despite surgery and antimycotic therapy the patient died from circulation failure. The fungus is compared to several related Exophiala species. ...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1991.tb00651.x
更新日期:1991-05-01 00:00:00
abstract::Fifty-four patients with human immunodeficiency virus (HIV) infection were studied to assess the load of oral carriage of Candida spp. The mean oral Candida carriage density (30,305.93 +/- 56,643.93 CFU ml(-1)) in HIV patients was significantly higher than that seen in the control population (93.48 +/- 358.48 CFU ml(-...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2008.01534.x
更新日期:2009-01-01 00:00:00
abstract::The sensitivity of a sandwich enzyme-linked immunosorbent assay (ELISA) for detecting Aspergillus galactomannan was evaluated with 66 serum samples and 113 specimens of the respiratory tract obtained from 52 patients with pulmonary diseases. The patients were divided into five groups: proven invasive pulmonary aspergi...
journal_title:Mycoses
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Paracoccidioidomycosis is a deep mycosis endemic to Latin America, with considerable morbidity and mortality. It is caused by the dimorphic fungus Paracoccidioides brasiliensis, which affects, among other organs in the human body, the oral cavity. Fungus virulence and immunocompetence of the host determine the establi...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/myc.12017
更新日期:2013-05-01 00:00:00
abstract::Chronic vegetating pyoderma is usually associated with staphylococci and streptococci. A case that started as a therapeutically resistant tinea of the hand caused by Trichophyton mentagrophytes is described. The vegetating skin lesion was associated with low immunoglobulin levels and impaired phagocytosis. It healed a...
journal_title:Mycoses
pub_type: 信件
doi:10.1111/j.1439-0507.2006.01191.x
更新日期:2006-03-01 00:00:00
abstract::The antifungal activities of extracts of 10 medicinal plants collected from south-eastern parts of Nigeria were tested against seven pathogenic fungi using the broth dilution and agar plate methods. All the extracts at 1:10 dilution inhibited the growth of Basidiobolus haptosporus and B. ranarum but did not inhibit th...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1995.tb00048.x
更新日期:1995-05-01 00:00:00
abstract::Vulvovaginal candidiasis (VVC) is a hormonal-dependent infection but in contrast to sporadic VVC, therapy of recurrent vulvovaginal candidiasis (RVVC) is still unsolved. Long-term administration of medroxyprogesterone acetate was evaluated for the management of RVVC. Overall, 20 patients were treated with Depo-Provera...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.12593
更新日期:2017-04-01 00:00:00
abstract::For anthropophilic tinea capitis (TC), household spread and asymptomatic scalp carriage (ASC) is considered an important route of transmission and incomplete clearance. To investigate ASC in household contacts of patients diagnosed with TC in a tertiary hospital in Athens, Greece, we retrospectively reviewed the medic...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.12166
更新日期:2014-06-01 00:00:00
abstract::Invasive aspergillosis has increasingly been recognised to cause significant morbidity and mortality in immunocompromised patients. Fever unresponsive to broad-spectrum antibiotics is the earliest and most common sign of an invasive fungal infection. As invasive Aspergillus infections are usually acquired by inhalatio...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0507.1997.tb00559.x
更新日期:1997-01-01 00:00:00
abstract::CHROMagar Candida is a new differential culture medium that allows selective isolation of yeasts and simultaneously identifies colonies of Candida albicans, Candida glabrata, Candida tropicalis and Candida krusei. We evaluated this medium and compared it with a reference medium, Sabouraud glucose agar, for the presump...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1046/j.1439-0507.1999.00406.x
更新日期:1999-04-01 00:00:00