Abstract:
AIMS/HYPOTHESIS:Increased oxidative-nitrosative stress, poly(ADP-ribose) polymerase (PARP) activation and subsequent cellular damage play important roles in the complications of both diabetes mellitus and pregnancy. Our aim was to investigate nitrative stress and PARP activity levels during normal and gestational diabetic (GDM) pregnancy in both maternal and fetal tissues. METHODS:Blood samples were collected during pregnancy (weeks 16-29 and 36-40), and placental and umbilical cord tissues were harvested after delivery from healthy volunteers and GDM patients subjected to a carbohydrate-restricted diet or insulin treatment. Immunohistochemical staining was performed on leucocytes and tissue sections using anti-nitrotyrosine (NT), anti-poly(ADP-ribose) (PAR) and anti-apoptosis inducing factor antibodies. RESULTS:In healthy pregnancies the intensity of NT and PAR staining of leucocytes correlated positively with gestational week (R (2) = 0.43, p < 0.01 and R (2) = 0.49, p < 0.001, respectively). In patients on a carbohydrate-restricted diet PAR staining was already strong in weeks 16-29 (p < 0.001 vs control) and did not increase further. In weeks 16-29 there was a correlation between PAR staining and the 2 h value of the oral glucose tolerance test (R (2) = 0.49, p < 0.001). Patients with the highest level of leucocyte PARP activity later required insulin therapy, which decreased the intensity of NT and PAR staining. Placental and umbilical cord tissues also had a higher level of nitrative stress markers in GDM pregnancies, but the highest level of PARP activity was observed after insulin therapy. CONCLUSIONS/INTERPRETATION:Continuous elevation of tyrosine nitration and PARP activation may be considered physiological during pregnancy. However, the high level of PARP activity in early pregnancy may signal the subsequent development of severe GDM.
journal_name
Diabetologiajournal_title
Diabetologiaauthors
Horváth EM,Magenheim R,Kugler E,Vácz G,Szigethy A,Lévárdi F,Kollai M,Szabo C,Lacza Zdoi
10.1007/s00125-009-1435-3subject
Has Abstractpub_date
2009-09-01 00:00:00pages
1935-43issue
9eissn
0012-186Xissn
1432-0428journal_volume
52pub_type
杂志文章相关文献
DIABETOLOGIA文献大全abstract:AIMS/HYPOTHESIS:Prolactin, a multifunctional hormone, is involved in regulating insulin sensitivity and glucose homeostasis in experimental studies. However, whether circulating concentrations of prolactin are associated with risk of type 2 diabetes remains uncertain. METHODS:We analysed the prospective relationship b...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-018-4733-9
更新日期:2018-12-01 00:00:00
abstract::An incidence of 9.0 out of 100,000 children under 19 years of age with Type 1 (insulin-dependent) diabetes was detected in Toronto during a 2 year prospective study. An increased number of cases occurred in the winter months of one of the years but not of the other during the study. The annual incidence was the same i...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00281308
更新日期:1982-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:No previous studies on the association between salt intake and the risk of type 2 diabetes have been reported. The aim of this study was to assess whether high salt intake, measured by 24-h urinary sodium excretion, is an independent risk factor for type 2 diabetes. METHODS:We followed prospectively 93...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-005-1824-1
更新日期:2005-08-01 00:00:00
abstract:AIMS/HYPOTHESIS:Insulin clearance may decline as an early mechanism compensating for deteriorating insulin sensitivity. However, no previous studies have investigated the association between subclinical inflammation or impaired fibrinolysis and insulin clearance. We examined the association between plasminogen activato...
journal_title:Diabetologia
pub_type: 杂志文章,多中心研究
doi:10.1007/s00125-012-2741-8
更新日期:2013-01-01 00:00:00
abstract:AIMS/HYPOTHESIS:Glucagon-like peptide-1 (GLP-1), a polypeptide hormone secreted by the l-cells in the gastrointestinal tract, has shown promising effects as a new treatment modality for patients with Type II (non-insulin-dependent) diabetes mellitus. However, the pharmacokinetic profile of native GLP-1 with a rapid eli...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s00125-001-0719-z
更新日期:2002-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:Abnormal rheological properties of erythrocytes, leucocytes and plasma may have a role in the development of diabetic microangiopathy. We hypothesized that changed haemorrheological variables may already be found in children with onset diabetes. METHODS:Erythrocyte deformation (rheoscope), neutrophil d...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250051239
更新日期:1999-07-01 00:00:00
abstract:AIMS/HYPOTHESIS:Diabetic distal symmetrical sensory polyneuropathy (DSP) affects 20-30% of diabetic patients. Transcutaneous electrical nerve stimulation (TENS) and electrical spinal cord stimulation have been proposed as physical therapies. We performed a controlled, randomised pilot trial to compare the effects of hi...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s00125-005-1728-0
更新日期:2005-05-01 00:00:00
abstract:AIMS/HYPOTHESIS:Circulating levels of adiponectin are negatively associated with multiple indices of insulin resistance, and the concentration is reduced in humans with insulin resistance and type 2 diabetes. However, the mechanisms by which adiponectin improves insulin sensitivity remain unclear. SUBJECTS AND METHODS...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-006-0240-5
更新日期:2006-06-01 00:00:00
abstract:AIMS/HYPOTHESIS:To identify the possible causes of the lengthening of the action potential duration described in patients affected by diabetes mellitus. METHODS:We studied the effects of streptozotocin-induced diabetes on the current density of the repolarising potassium currents It(o), IK, Iss and IK1 in enzymaticall...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250050013
更新日期:2000-01-01 00:00:00
abstract:AIMS/HYPOTHESIS:Low phosphate and high calcium concentrations have been linked to altered glucose tolerance and reduced insulin sensitivity in non-diabetic individuals. The aim of this study was to examine the relationships of calcium and phosphate levels and the calcium-phosphate product with the development of type 2...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-014-3241-9
更新日期:2014-07-01 00:00:00
abstract:AIMS/HYPOTHESIS:The aim of the study was to analyse how strongly commuting and leisure-time physical activity affect progression to diabetes and to study whether this relationship is different in individuals with isolated impaired fasting glucose (i-IFG) and isolated impaired glucose tolerance (i-IGT). METHODS:We stud...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-009-1587-1
更新日期:2010-01-01 00:00:00
abstract:AIMS/HYPOTHESIS:The endogenous production of stromal cell-derived factor-1 (SDF-1) in beta cells in transgenic mice attenuates the development of diabetes in response to streptozotocin. Here we propose that beta cell injury induces SDF-1 production, and the SDF-1/chemokine (C-X-C motif) receptor 4 (CXCR4) interaction a...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-011-2181-x
更新日期:2011-08-01 00:00:00
abstract:AIMS/HYPOTHESIS:Carbohydrate-free diet prevents hyperglycaemia and beta cell destruction in the New Zealand Obese (NZO) mouse model. Here we have used a sequential dietary regimen to dissociate the effects of obesity and hyperglycaemia on beta cell function and integrity, and to study glucose-induced alterations of key...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-010-1973-8
更新日期:2011-03-01 00:00:00
abstract::Serum insulin response to a single bolus of IV glucose or tolbutamide was measured in eight healthy subjects. Insulin disappearance rate was assessed by deconvolution from the serum insulin levels, using the measured insulin disappearance rate. The mean rate constant of insulin disappearance was 0.238 +/- 0.005 min-1 ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00421232
更新日期:1978-09-01 00:00:00
abstract:UNLABELLED:AIMS/HYPOTHESIS; The aim of the Diabetes Prevention Study is to assess the efficacy of an intensive diet-exercise programme in preventing or delaying Type II (non-insulin-dependent) diabetes mellitus in subjects with impaired glucose tolerance, to evaluate the effects of the intervention programme on cardiov...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1007/s001250051229
更新日期:1999-07-01 00:00:00
abstract::This study aimed at evaluating the influence of furosemide upon insulin and glucagon responses to arginine in healthy subjects. For this purpose, six normal subjects received two consecutive arginine pulses (3 g), 60 min apart, before and after the administration of furosemide (40 mg, IV). The acute insulin response (...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00251008
更新日期:1980-04-01 00:00:00
abstract::In this study, we found that the ratio of proinsulin to total immunoreactive insulin was much higher in 22 patients with Type 2 (non-insulin-dependent) diabetes mellitus than in 28 non-diabetic control subjects of similar age and adiposity (32 +/- 3 vs 15 +/- 1%, p less than 0.001). In addition, the arginine-induced a...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00296991
更新日期:1987-09-01 00:00:00
abstract:AIM/HYPOTHESIS:A strong association between susceptibility to type 2 diabetes and common variants of transcription factor 7-like 2 (TCF7L2), encoding an enteroendocrine transcription factor involved in glucose homeostasis, has been reported in three different populations (Iceland, Denmark and USA) by Grant et al. We ai...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-006-0477-z
更新日期:2007-01-01 00:00:00
abstract::The adrenergic regulation of adipose tissue lipolysis in response to insulin-induced hypoglycaemia (intravenous infusion of soluble insulin 0.10 IU.kg body weight-1.h-1 until the arterial plasma glucose fell below 2.8 mmol/l) was investigated directly in vivo in 11 insulin-dependent diabetic (IDDM) patients and 12 con...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250050519
更新日期:1996-07-01 00:00:00
abstract:AIM/HYPOTHESIS:Although mitochondrial oxidative phosphorylation (OxPhos) dysfunction is believed to be responsible for beta cell dysfunction in insulin resistance and mitochondrial diabetes, the mechanisms underlying progressive beta cell failure caused by defective mitochondrial OxPhos are largely unknown. METHODS:We...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-015-3506-y
更新日期:2015-04-01 00:00:00
abstract::Short- and long-term regulation of hepatic carbohydrate metabolism by insulin-like growth factor II was studied in primary cultures of adult rat hepatocytes and compared to the metabolic potency of insulin. Insulin-like growth factor II stimulated glycogen synthesis from [14C]glucose, uptake of [3H]aminoisobutyric aci...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00400920
更新日期:1992-03-01 00:00:00
abstract:AIMS/HYPOTHESIS:We assessed secular trends of cardiovascular outcomes following first diagnosis of myocardial infarction (MI) or diabetes in an unselected population. METHODS:All Danish residents aged > or = 30 years without prior diabetes or MI were identified by individual-level linkage of nationwide registers. Indi...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-010-1783-z
更新日期:2010-08-01 00:00:00
abstract:AIMS/HYPOTHESIS:Oral administration of antigen can induce immunological tolerance. Insulin is a key autoantigen in childhood type 1 diabetes. Here, oral insulin was given as antigen-specific immunotherapy before the onset of autoimmunity in children from age 6 months to assess its safety and immune response actions on ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-020-05376-1
更新日期:2021-01-30 00:00:00
abstract:AIMS/HYPOTHESIS:HLA haplotypes DRB1*03_DQB1*02 and DRB1*04_DQB1*0302, and allelic variation of the T cell regulatory gene cytotoxic T-lymphocyte-associated antigen-4 (CTLA4) and of the T cell activation gene protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22) have been associated with type 1 diabetes...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-007-0603-6
更新日期:2007-04-01 00:00:00
abstract::Hyperglycaemia may enhance insulin resistance typical of non-insulin dependent diabetes mellitus, as well as insulin dependent diabetes mellitus, and thus initiate a vicious pathogenetic cycle. We sought to test the hypothesis that reduction in chronic hyperglycaemia in the diabetic dog by methods that do not employ i...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00290590
更新日期:1988-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:Dysfunction of lipid metabolism in white adipose tissue can substantially interfere with health and quality of life, for example in obesity and associated metabolic diseases. Therefore, it is important to characterise pathways that regulate lipid handling in adipocytes and determine how they affect meta...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-016-4152-8
更新日期:2017-02-01 00:00:00
abstract::The development of diabetic glomerulopathy in kidneys transplanted to diabetic patients was estimated in transplant biopsies and evaluated in relation to suspected clinical risk factors for diabetic nephropathy. Surgical biopsies were taken at baseline and at 24-36 months post-transplantation in 16 Type 1 (insulin-dep...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00400997
更新日期:1991-09-01 00:00:00
abstract::We have suggested that the order of cellular vascular perfusion within the islet is important in the regulation of islet hormone secretion. Anatomically, the A and D cells appear to be randomly dispersed throughout the mantle. Although islet capillary blood flow is known to be from the B-cell core to the A- and D-cell...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00265095
更新日期:1989-03-01 00:00:00
abstract::For many, the extent to which blood glucose control can be lowered is limited by risk of hypoglycaemia. Hypoglycaemia is feared and carries fiscal, social and medical costs, with risk of death being associated with severe hypoglycaemia in patients with type 2 diabetes at high cardiovascular risk. In this issue of Diab...
journal_title:Diabetologia
pub_type: 评论,杂志文章
doi:10.1007/s00125-015-3550-7
更新日期:2015-06-01 00:00:00
abstract:AIMS/HYPOTHESIS:Our aim was to compare the therapeutic effect of thalidomide and rosiglitazone on the prevention of diabetic retinopathy in streptozotocin-induced diabetic rats. METHODS:Male Holtzman rats of 6 to 8 weeks of age and weighing 170+/-30 g were randomly divided into four groups: control ( n=13), untreated ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-003-1234-1
更新日期:2003-12-01 00:00:00