Abstract：
BACKGROUND:Myeloid and plasmacytoid dendritic cell (DC) subsets have been recently identified in the human lung based on their differential expression of Blood DC Antigens 1-3 (BDCAs). We investigated the expression of these antigens by isolated human pulmonary CD1a(+) DCs, namely Langerhan's cells. METHODS:Using an in vitro cell culture system we successfully isolated a population of relatively pure (>70%) CD1a(+) cells from human lung tissue (n=5 subject samples) and stained these with antibodies against the myeloid DC markers BDCA1 (CD1c) and BDCA3 (CD303), the plasmacytoid DC marker BDCA2 (CD141), the Langerhan's cell marker Langerin and the maturation marker CD83. RESULTS:Among different subject samples, the isolated CD1a(+) cells showed variable expression of Langerin, BDCAs and CD83. Interestingly, in two subject samples, which contained >70% CD83(+) mature CD1a(+) cells, >50% of the cells were positive for all of the BDCAs. CONCLUSIONS:We conclude that isolated pulmonary CD1a(+) DCs in vitro have the capacity to express both myeloid and plasmacytoid BDCA markers and that rather than subset restriction in pulmonary DCs, a significant degree of flexibility/plasticity can be induced, albeit experimentally.
journal_name
Respir Medjournal_title
Respiratory medicineauthors
Tsoumakidou M,Kemp SJ,Thorley AJ,Zhu J,Dewar A,Jeffery PK,Tetley TDdoi
10.1016/j.rmed.2009.02.006subject
Has Abstractpub_date
2009-06-01 00:00:00pages
935-8issue
6eissn
0954-6111issn
1532-3064pii
S0954-6111(09)00058-4journal_volume
103pub_type
杂志文章abstract：:The diagnostic value of isocapnic hyperventilation of cold air (IHCA) is not fully established. All 342 adult patients in whom IHCA had been performed because of a clinical suspicion of asthma between 1992 and 1994 were analysed retrospectively in the authors' hospital. In addition, 26 healthy subjects were recruited....
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/s0954-6111(97)90112-8
更新日期：1997-09-01 00:00:00
abstract：:Recent developments in intrabronchial administration of insulin raise lung function in patients with type I diabetes as important issue. Several studies in adults report abnormalities of lung function of these patients. The aim of this study was to investigate lung function in children with type I diabetes. Twenty-sev...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1053/rmed.2002.1402
更新日期：2002-12-01 00:00:00
abstract：INTRODUCTION:Non-cystic fibrosis(CF) bronchiectasis has been recognized in children for the past 200 years. Early childhood pneumonia and underlying conditions such as immunodeficiency, primary ciliary dyskinesia(PCD), and congenital lung pathology should be considered in the etiology. The aim of our study was to descr...
journal_title：Respiratory medicine
pub_type： 杂志文章,评审
doi：10.1016/j.rmed.2018.02.013
更新日期：2018-04-01 00:00:00
abstract：:In this study healthy never-smoking subjects (n = 18) were recruited from a population study. Bronchoalveolar lavage (BAL), blood lymphocytes and bronchial biopsies, analysed both in the epithelium and lamina propria, were stained for T and B lymphocytes, natural killer (NK) cells and different subpopulations of T lym...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/s0954-6111(99)90156-7
更新日期：1999-08-01 00:00:00
abstract：BACKGROUND:Sarcoidosis patients often are troubled by dyspnea, exercise limitation, and fatigue. Many patients (up to 50-81%) suffer from sarcoidosis-associated fatigue. The etiology of fatigue in sarcoidosis is still unclear. OBJECTIVE:The aim of this study was to assess the relationship between fatigue and both exer...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2016.05.010
更新日期：2016-07-01 00:00:00
abstract：:Bird fancier's lung, the most common form of extrinsic allergic alveolitis in Britain, can be a difficult diagnostic problem. The symptoms are non-specific, often insidious in onset and frequently misdiagnosed as influenza or a viral or bacterial pneumonia. Frequently there is a delay in eliciting the history of expos...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/s0954-6111(06)80230-1
更新日期：1992-03-01 00:00:00
abstract：:Chlorofluorocarbon (CFC)-containing inhalers for use in the treatment of asthma are to be phased out under the terms of the Montreal Protocol (1). In this multi-centre, randomized, double-blind study, the therapeutic equivalence of two formulations of beclomethasone dipropionate (BDP) containing CFC or non-CFC (HFA134...
journal_title：Respiratory medicine
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1053/rmed.1999.0678
更新日期：2000-01-01 00:00:00
abstract：INTRODUCTION:Primary ciliary dyskinesia (PCD) is a rare genetically heterogeneous disorder of motile cilia, which leads to recurrent and chronic airway infections. Detailed information about infection causing pathogens is scarce. With this study, we aimed to determine the prevalence and susceptibility of the most commo...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2019.08.009
更新日期：2019-09-01 00:00:00
abstract：:Preterm infants who are symptomatic at follow-up frequently have a high airways resistance (RAW) and a low functional residual capacity to thoracic gas volume ratio (FRC:TGV). The aim of this study was to assess the effect of bronchodilator therapy on these lung function abnormalities. Thirty-four infants who had recu...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/0954-6111(94)90057-4
更新日期：1994-04-01 00:00:00
abstract：:Active smoking is an increasing problem amongst U.K. teenagers. The smoking habits of a cohort of 14-16-year-olds were determined and the association between regular active smoking and domestic and social factors investigated. Current smoking habits of a cohort of 2289 14-16-year-olds were assessed using a simple post...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1053/rmed.1999.0746
更新日期：2000-04-01 00:00:00
abstract：:In 16 patients with pulmonary fibrosis, an artificial pneumothorax was introduced using the Veress cannula and the Saugman water manometer. Atmospheric air was introduced by fractionated insufflation to a total volume of 800 ml (median). The interpleural space was found on the first attempt, and in all cases, fraction...
journal_title：Respiratory medicine
pub_type： 临床试验,杂志文章
doi：10.1016/s0954-6111(97)90253-5
更新日期：1997-08-01 00:00:00
abstract：:RANTES has been implicated in the allergic inflammation of asthma by promoting the migration and activation of the inflammatory cells, including eosinophils. The study was undertaken to evaluate RANTES levels in the exhaled breath condensate (EBC) of asthmatics with different degrees of asthma severity. EBC was collec...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2008.03.010
更新日期：2008-08-01 00:00:00
abstract：:The pathophysiology of corticosteroid-induced osteoporosis is complex, but centres around a primary inhibition of osteoblastic activity compounded by the effects of secondary hyperparathyroidism. The preservation of the microanatomy of bone in the early stages of the disease, coupled with strategies to prevent the eme...
journal_title：Respiratory medicine
pub_type： 杂志文章,评审
doi：10.1016/s0954-6111(05)80252-5
更新日期：1993-02-01 00:00:00
abstract：:Airway dehydration and subsequent hyperosmolarity of periciliary fluid are considered critical events in exercise-induced bronchoconstriction (EIB). It has been shown that an in vitro hyperosmolar stimulation of basophils and mast cells with mannitol can induce the release of histamine and leukotrienes. The aim of thi...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/s0954-6111(03)00119-7
更新日期：2003-08-01 00:00:00
abstract：OBJECTIVES:To analyze the conditions of psychological dysfunction and positive mental health in patients with asthma and chronic bronchitis (CB), as compared to healthy individuals, and to identify the factors associated with these mental health indicators. METHODS:Cross-sectional study based on data obtained from the...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2012.03.021
更新日期：2012-07-01 00:00:00
abstract：BACKGROUND:Anti-endothelial cell antibodies (AECA) are circulating antibodies that bind to endothelial antigens and induce endothelial cell damage. AECA have been detected in patients with collagen vascular disease (CVD) and their presence is associated with interstitial lung disease (ILD) in cases of CVD. However, the...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2007.07.024
更新日期：2008-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Mycobacterium tuberculosis has been proposed as a candidate agent for the cause of sarcoidosis. The QuantiFERON-TB Gold test has a higher specificity for detecting M. tuberculosis infection than the conventional tuberculin skin test. This study aimed to investigate the rate of positive QuantiFE...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2007.09.013
更新日期：2008-02-01 00:00:00
abstract：BACKGROUND:A simple rule based on short-acting inhaled β2-agonist (SABA) use could identify patients with chronic obstructive pulmonary disease (COPD) at increased risk of exacerbations and signal the need for maintenance therapy change, similar to asthma "Rules of Two(®)". METHODS:Associations between SABA use, COPD ...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2014.07.002
更新日期：2014-09-01 00:00:00
abstract：:This retrospective study reviews the patterns of P. cepacia pulmonary infection in 75 of a total of 872 mainly adults with cystic fibrosis, registered here during the 4 years 1987-1990; 35 (47%) were female. During this period, 55 patients acquired P. cepacia and the annual incidence and prevalence rates have remained...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/0954-6111(93)90090-m
更新日期：1993-04-01 00:00:00
abstract：INTRODUCTION:Non-invasive positive pressure ventilation (NIPPV) is an effective treatment in respiratory failure. Continuous positive pressure (CPAP) may also be useful in acute hypoxaemic patients. Supplementary oxygen is usually necessary in both systems. However, the inspired oxygen fraction (FiO2) delivered by a NI...
journal_title：Respiratory medicine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.rmed.2005.12.005
更新日期：2006-09-01 00:00:00
abstract：AIM:To examine tobacco use among teenagers, identify factors related to tobacco use, as well as evaluate the outcome of a smoking prevention program. METHODS:From age 7/8 to 14/15, annual questionnaires about asthma and allergy have been completed in the OLIN paediatric study in Northern Sweden. From 12/13 years, ques...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2006.07.001
更新日期：2007-03-01 00:00:00
abstract：:Seventy-seven patients with morbid obesity, body mass index (BMI) 40-69.9 kg m(-2), who were candidates for gastroplasty, were studied in our laboratory as part of a pre-operative survey. They had no complaints other than obesity and were not cyanotic. A group of 28 lean subjects (BMI 20-29.8 kg m(-2)) who were candid...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1053/rmed.2001.1035
更新日期：2001-04-01 00:00:00
abstract：BACKGROUND:International guidelines recommend simple spirometry for bronchiectasis patients. However, pulmonary pathophysiology of bronchiectasis is very complex and still poorly understood. Our objective was to characterize lung function in bronchiectasis and identify specific functional sub-groups. METHODS:This was ...
journal_title：Respiratory medicine
pub_type： 杂志文章,多中心研究
doi：10.1016/j.rmed.2018.10.031
更新日期：2018-12-01 00:00:00
abstract：:Viozan (sibenadet HCl, AR-C68397AA) is a novel dual D2 dopamine receptor, beta2-adrenoceptor agonist that has been investigated for efficacy in alleviating the symptoms of chronic obstructive pulmonary disease (COPD). The slowly progressive nature of this disease means that patients will require ongoing therapeutic ma...
journal_title：Respiratory medicine
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：
更新日期：2003-01-01 00:00:00
abstract：BACKGROUND:Exhaled breath condensate (EBC) is simple to collect and as such a non-invasive method that has attracted substantial interest in the last few years. However, several methodological concerns have been raised and it has been difficult to reproduce results between different centres. Because of low concentratio...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2005.04.007
更新日期：2006-01-01 00:00:00
abstract：BACKGROUND:This study evaluated the dose-response and dosing interval of the novel long-acting muscarinic receptor antagonist (LAMA) GSK573719 in patients with COPD. METHODS:This randomized, double-blind, placebo-controlled, 3-way cross-over, incomplete block study evaluated 5 once-daily doses of GSK573719 (62.5-1000 ...
journal_title：Respiratory medicine
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.rmed.2012.03.012
更新日期：2012-07-01 00:00:00
abstract：BACKGROUND:Gas6 protein is involved in pulmonary embolism (PE) and acute inflammation in animal models. METHODS:We enrolled 82 consecutive patients with acute dyspnea and suspected PE (Geneva score with high (HCP) or low/intermediate clinical probability (LICP)+D-dimer >or=0.5microg/mL) and 29 age-matched healthy volu...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2008.10.018
更新日期：2009-04-01 00:00:00
abstract：:Collection of airway calibre and beta2-agonist data in large clinical trials and epidemiological surveys is sometimes difficult and may be an inefficient use of resources. The aim of this study was to determine whether the omission of the forced expiratory volume in 1 sec (FEV1) and beta2-agonist questions from the se...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1053/rmed.2001.1034
更新日期：2001-05-01 00:00:00
abstract：:The Lion Intoximeter 3000 has been used for evidential breath testing in the U.K. for some years. Some individuals with lung diseases have difficulty in providing evidential breath samples using the device. This study describes an investigation that we have carried out on a newer instrument--the Lion Intoxilyzer 6000U...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1053/rmed.2000.0797
更新日期：2000-07-01 00:00:00
abstract：BACKGROUND:Stepping down from combination asthma therapy (inhaled corticosteroids (ICS) + long-acting β2 agonists (LABA)) is often avoided due to fear of exacerbations, which may lead to overmedication in well-controlled asthma. A better knowledge about the predictors of outcome might encourage clinicians to start step...
journal_title：Respiratory medicine
pub_type： 临床试验,杂志文章
doi：10.1016/j.rmed.2016.06.010
更新日期：2016-08-01 00:00:00
