Abstract：
BACKGROUND:Exhaled breath condensate (EBC) is simple to collect and as such a non-invasive method that has attracted substantial interest in the last few years. However, several methodological concerns have been raised and it has been difficult to reproduce results between different centres. Because of low concentrations of inflammatory markers, potential loss in the sampling system may have great influence. The aim of the present study was to se if evaporation and plastic coating could facilitate detection. METHODOLOGY:Through methodological improvements, we have now made it possible to measure EBC concentrations of eicosanoids and cytokines in our system. Due to absorbance of both fatty acid derivates and proteins to several plastics, the first step is coating of all surfaces with bovine serum albumin and Tween 20. Since several assays are sensitive to these factors, the methodology has to be standardised to avoid false results. Secondly, larger amounts of EBC have to be vacuum-dried, and thereafter resolved in the respective assay buffers. The EBCs have to be concentrated 5-10 times, depending on samples and assay sensitivity. RESULTS:Due to these improvements we can measure, for example, cysteinyl-leukotrienes, leukotriene B4, prostaglandin E and 8-isoprostane. High sensitivity assays have also made it possible to measure cytokines, for example, interleukin (IL)-1beta, IL-8 and IL-13. SUMMARY:We are aware of different results from other labs. However, it seems essential to coat and to concentrate the samples in order to achieve reliable and measurable results.
journal_name
Respir Medjournal_title
Respiratory medicineauthors
Tufvesson E,Bjermer Ldoi
10.1016/j.rmed.2005.04.007subject
Has Abstractpub_date
2006-01-01 00:00:00pages
34-8issue
1eissn
0954-6111issn
1532-3064pii
S0954-6111(05)00161-7journal_volume
100pub_type
杂志文章abstract：BACKGROUND:In patients with chronic obstructive pulmonary disease (COPD), energy expenditure (EE) assessment during the performance of daily activities is not yet studied in depth. The aim of this study was to determine which daily activities are more demanding to patients with COPD and to compare the accuracy of EE es...
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pub_type： 杂志文章
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pub_type： 杂志文章,评审
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pub_type： 杂志文章,随机对照试验
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pub_type： 杂志文章,多中心研究,随机对照试验
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pub_type： 临床试验,杂志文章
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更新日期：2014-02-01 00:00:00
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pub_type： 杂志文章,随机对照试验
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更新日期：2019-01-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：1998-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.rmed.2009.10.004
更新日期：2010-03-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2002-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：2011-03-01 00:00:00
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journal_title：Respiratory medicine
pub_type： 杂志文章,多中心研究,随机对照试验
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pub_type： 杂志文章
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更新日期：2014-11-01 00:00:00
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pub_type： 杂志文章,多中心研究
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更新日期：2018-12-01 00:00:00
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更新日期：2009-07-01 00:00:00
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journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/s0954-6111(98)90103-2
更新日期：1998-02-01 00:00:00
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journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2005.05.004
更新日期：2006-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.rmed.2012.03.011
更新日期：2012-07-01 00:00:00
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pub_type： 杂志文章,多中心研究,随机对照试验
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更新日期：2006-04-01 00:00:00
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journal_title：Respiratory medicine
pub_type： 杂志文章,评审
doi：10.1016/j.rmed.2016.12.009
更新日期：2017-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：1999-03-01 00:00:00
