Comparison of the effect of amniotic membrane suspension and autologous serum on alkaline corneal epithelial wound healing in the rabbit model.


PURPOSE:Alkaline corneal epithelial wound closure rates in rabbit eyes were compared after topical applications of amniotic membrane suspension, autologous serum, or preservative-free artificial tears. METHODS:Thirty rabbits were divided into 3 randomization groups of 10 rabbits each. Central corneal alkali wound was produced in both eyes of the rabbits by applying a 5-mm round filter paper, soaked in 1 N NaOH, for 60 seconds. Group 1 animals received preservative-free artificial tears in 1 eye and amniotic membrane suspension in the other eye; group 2 animals received preservative-free artificial tears in 1 eye and autologous serum in the other eye; and group 3 animals received autologous serum in 1 eye and amniotic membrane suspension in the other eye. The treatment was dosed 4 times a day with the eyedrops, and epithelial wound closure was recorded using slit lamp. The data were analyzed to determine the rate of wound closure. RESULTS:The mean wound radius closure rate was 67.8 +/- 5.2 microm/h for autologous serum-treated eyes, 74.5 +/- 5.4 microm/h for amniotic membrane suspension-treated eyes, and 66.8 +/- 5.0 microm/h for preservative-free artificial tear-treated eyes. CONCLUSIONS:This study shows that alkali-injured corneal epithelial wounds heal faster when treated with amniotic membrane suspension than with autologous serum or preservative-free artificial tears.






Shahriari HA,Tokhmehchi F,Reza M,Hashemi NF




Has Abstract


2008-12-01 00:00:00
















  • Prolonged impairment of corneal innervation after exposure to sulfur mustard and its relation to the development of delayed limbal stem cell deficiency.

    abstract:PURPOSE:Ocular injuries after exposure to the vesicant sulfur mustard (SM) are characterized by acute corneal erosions and inflammation of the anterior segment that may be followed by delayed limbal stem cell deficiency (LSCD), expressed clinically by corneal neovascularization and epithelial defects. The present study...


    pub_type: 杂志文章


    authors: Kadar T,Dachir S,Cohen M,Gutman H,Cohen L,Brandeis R,Horwitz V,Amir A

    更新日期:2013-04-01 00:00:00

  • White-to-white corneal diameter in the Tehran Eye Study.

    abstract:PURPOSE:To determine the mean corneal diameter and its determinants in the population of Tehran. METHODS:From the Tehran Eye Study participants who were sampled through a stratified cluster approach, those in the first 4 municipality districts of Tehran were selected, and after applying the inclusion and exclusion cri...


    pub_type: 杂志文章,多中心研究


    authors: Hashemi H,KhabazKhoob M,Yazdani K,Mehravaran S,Mohammad K,Fotouhi A

    更新日期:2010-01-01 00:00:00

  • Randomized clinical study for comparative evaluation of fourth-generation fluoroquinolones with the combination of fortified antibiotics in the treatment of bacterial corneal ulcers.

    abstract:PURPOSE:Comparative evaluation of efficacy of monotherapy with moxifloxacin (0.5%) or gatifloxacin (0.3%) with combination therapy of cefazolin (5%) and tobramycin (1.3%) in treatment of bacterial corneal ulcers. METHODS:Patients diagnosed with bacterial keratitis (ulcer diameter 2-8 mm) were randomized to 1 of the 3 ...


    pub_type: 杂志文章,随机对照试验


    authors: Shah VM,Tandon R,Satpathy G,Nayak N,Chawla B,Agarwal T,Sharma N,Titiyal JS,Vajpayee RB

    更新日期:2010-07-01 00:00:00

  • Refractive Surgery: Malpractice Litigation Outcomes.

    abstract:PURPOSE:To review data on malpractice claims related to refractive surgery to identify common allegations and injuries and financial outcomes. METHODS:The WestlawNext database was reviewed for all malpractice lawsuits/settlements related to refractive eye surgery. Data evaluated included patient demographics, type of ...


    pub_type: 杂志文章


    authors: Custer BL,Ballard SR,Carroll RB,Barnes SD,Justin GA

    更新日期:2017-10-01 00:00:00

  • Nontraumatic corneal perforation.

    abstract:PURPOSE:To study the predisposing conditions, treatments, and visual outcomes of nontraumatic corneal perforations. METHODS:A retrospective chart review was conducted of all nontraumatic corneal perforations seen between January 1992 and December 1998, with > or = 3 months of follow-up, at the Cornea Service Wills Eye...


    pub_type: 杂志文章


    authors: Lekskul M,Fracht HU,Cohen EJ,Rapuano CJ,Laibson PR

    更新日期:2000-05-01 00:00:00

  • Six different mutations of TGFBI (betaig-h3, keratoepithelin) gene found in Japanese corneal dystrophies.

    abstract:PURPOSE:To investigate mutations of the human transforming growth factor beta-induced gene (TGFBI), transforming growth factor-beta-induced gene product (betaig-h3, keratoepithelin), in Japanese patients with Avellino corneal dystrophy (ACD), lattice corneal dystrophy (LCD), granular corneal dystrophy (GCD), and Reis-B...


    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Fujiki K,Hotta Y,Nakayasu K,Yamaguchi T,Kato T,Uesugi Y,Ha NT,Endo S,Ishida N,Lu WN,Kanai A

    更新日期:2000-11-01 00:00:00

  • Descemet-stripping automated endothelial keratoplasty by using suture for donor insertion.

    abstract:PURPOSE:To describe a new technique for donor insertion in cases of Descemet-stripping automated endothelial keratoplasty (DSAEK). METHODS:This is a retrospective case series study of a novel method to introduce the donor tissue in DSAEK. This is a report of 16 cases that underwent the DSAEK procedure. The surgical te...


    pub_type: 杂志文章


    authors: Sarnicola V,Toro P

    更新日期:2008-08-01 00:00:00

  • Comparative study of descemet stripping automated endothelial keratoplasty donor preparation by Moria CBm microkeratome, horizon microkeratome, and Intralase FS60.

    abstract:PURPOSE:To evaluate the quality of stromal bed and the safety on endothelium in preparation of donor tissue for Descemet stripping automated endothelial keratoplasty in a masked fashion using 2 mechanical microkeratomes and a femtosecond laser. METHODS:Deep anterior lamellar dissection was performed on 15 donor cornea...


    pub_type: 杂志文章


    authors: Mootha VV,Heck E,Verity SM,Petroll WM,Lakshman N,Muftuoglu O,Bowman RW,McCulley JP,Cavanagh HD

    更新日期:2011-03-01 00:00:00

  • Cluster of Post-Operative Endophthalmitis Caused by Acanthamoeba T10 Genotype - A First Report.

    abstract:PURPOSE:To report a cluster of postoperative Acanthamoeba endophthalmitis after routine cataract surgeries. METHODS:A brief summary of sentinel events leading to the referral of 4 patients of postoperative endophthalmitis to our hospital is followed by clinical descriptions and the various diagnostic approaches and in...


    pub_type: 杂志文章


    authors: Rammohan R,Hajib Naraharirao M,Veerappan S,Vijayaraghavan P,Rajaraman R,Manayath GJ,Dsouza P,Radhakrishnan S,Venkatapathy N,Lakshmipathi D,Madhuravasal Krishnan J,Raghavan A

    更新日期:2021-02-01 00:00:00

  • Keratitis resulting from Thielavia subthermophila Mouchacca.

    abstract:PURPOSE:The purpose of this study was to report the first case of fungal keratitis resulting from Thielavia sp. METHODS:We conducted a retrospective chart review. RESULTS:A 10-year old girl presented 2 weeks after ocular plant injury with pain and corneal stromal infiltration with central ulceration and ill-defined m...


    pub_type: 杂志文章


    authors: Theoulakis P,Goldblum D,Zimmerli S,Muehlethaler K,Frueh BE

    更新日期:2009-10-01 00:00:00

  • A keratitis rat model for evaluation of anti-Acanthamoeba polyphaga agents.

    abstract:PURPOSE:To develop a rat model of chronic Acanthamoeba polyphaga keratitis suitable for pharmacologic assessment of therapeutic agents. METHODS:An A. polyphaga isolate (ATCC #50495) was grown in peptone-yeast extract-glucose medium. Five-weeks-old, Sprague-Dawley male rats were injected with 10(3) or 10(4) trophozoite...


    pub_type: 杂志文章


    authors: Vasseneix C,Gargala G,François A,Hellot MF,Duclos C,Muraine M,Benichou J,Ballet JJ,Brasseur G,Favennec L

    更新日期:2006-06-01 00:00:00

  • In vivo confocal microscopic findings of two siblings with Maroteaux-Lamy syndrome.

    abstract:PURPOSE:To describe the clinical and in vivo confocal microscopic findings of the cornea in 2 male siblings with Maroteaux-Lamy syndrome. METHODS:Two male siblings 27 and 22 years of age who had been diagnosed with Maroteaux-Lamy syndrome underwent ophthalmologic assessment. In vivo confocal microscopy of their cornea...


    pub_type: 杂志文章


    authors: Mocan MC,Eldem B,Irkec M

    更新日期:2007-01-01 00:00:00

  • Fungal keratitis caused by Scedosporium apiospermum: report of two cases and review of treatment.

    abstract:PURPOSE:We report our experience in treating two cases of Scedosporium apiospermum keratitis and provide a review of basic scientific and clinical data regarding the treatment of this visually devastating disease. METHOD:We present a case report and literature review. RESULTS:A 35-year-old woman and a 73-year-old man...


    pub_type: 杂志文章,评审


    authors: Wu Z,Ying H,Yiu S,Irvine J,Smith R

    更新日期:2002-07-01 00:00:00

  • The fate of scleral grafts in the cornea.

    abstract::There have been reports of the clearing of sclera that has been grafted into the cornea. This clearing was studied in the rabbit by transplanting full-thickness scleral buttons or by implanting slivers of sclera, both autografts and allografts, into the cornea. Some clearing around the edges of the implanted scleral t...


    pub_type: 杂志文章


    authors: Maurice DM,Singh T

    更新日期:1996-03-01 00:00:00

  • Conjunctival epithelial cells cultured on human amniotic membrane fail to transdifferentiate into corneal epithelial-type cells.

    abstract:PURPOSE:Previous studies on the use of human amniotic membrane (HAM) in rabbit stem cell deficiency models have found the new epithelium growing over the HAM to express cornea-specific keratins (K3 and K12) in 40% of the cases, suggesting that HAM may have induced conjunctival epithelial cells to transdifferentiate int...


    pub_type: 杂志文章


    authors: Cho BJ,Djalilian AR,Obritsch WF,Matteson DM,Chan CC,Holland EJ

    更新日期:1999-03-01 00:00:00

  • Outcomes of repeat endothelial keratoplasty in patients with failed deep lamellar endothelial keratoplasty.

    abstract:PURPOSE:To report the outcomes of repeat endothelial keratoplasty (EK) in patients with previous failed deep lamellar endothelial keratoplasty (DLEK). METHODS:This retrospective interventional case series involved the review of clinical records of patients with failed DLEK surgery who underwent repeat EK surgery. RES...


    pub_type: 杂志文章


    authors: Kim P,Brodbaker E,Lichtinger A,Yeung SN,Bahar I,Iovieno A,Amiran MD,Rootman DS

    更新日期:2011-11-01 00:00:00

  • Comparison of corneal surface higher-order aberrations after endothelial keratoplasty, femtosecond laser-assisted keratoplasty, and conventional penetrating keratoplasty.

    abstract:PURPOSE:To evaluate and compare corneal higher-order aberrations (HOAs) after Descemet stripping automated endothelial keratoplasty (DSAEK), femtosecond laser-assisted penetrating keratoplasty (FLAK), and conventional penetrating keratoplasty (PKP). METHODS:A retrospective comparison of consecutive surgical series of ...


    pub_type: 杂志文章


    authors: Chamberlain W,Omid N,Lin A,Farid M,Gaster RN,Steinert RF

    更新日期:2012-01-01 00:00:00

  • A review of relative risks associated with four types of contact lenses.

    abstract::This paper reviews the data of Frank et al. in the light of increasing concern over the safety of contact lenses used for extended wear. Relative risks were calculated for four types of contact lenses (CLs): extended wear soft, daily wear soft, rigid gas-permeable (RGP), and polymethylmethacrylate (PMMA) lenses--worn ...


    pub_type: 杂志文章


    authors: Bailey CS

    更新日期:1990-01-01 00:00:00

  • Intracameral moxifloxacin: in vitro safety on human ocular cells.

    abstract:PURPOSE:The fourth-generation fluoroquinolone, moxifloxacin, covers most gram-positive and gram-negative isolates causing endophthalmitis. It is safe and effective for systemic and topical use, but only limited data are available on prophylactic intracameral administration to prevent endophthalmitis. This study uses a ...


    pub_type: 杂志文章


    authors: Kernt M,Neubauer AS,Liegl RG,Lackerbauer CA,Eibl KH,Alge CS,Ulbig MW,A AK

    更新日期:2009-06-01 00:00:00

  • Corneal transplant rejection rate and severity after endothelial keratoplasty.

    abstract:PURPOSE:To examine the corneal transplant rejection rate and the rate of graft failure subsequent to rejection in the first 2 years after endothelial keratoplasty (EK) and to compare this to background data for similar cases of penetrating keratoplasty (PK). METHODS:Anonymized data from consecutive cases of EK [deep l...


    pub_type: 杂志文章,多中心研究


    authors: Allan BD,Terry MA,Price FW Jr,Price MO,Griffin NB,Claesson M

    更新日期:2007-10-01 00:00:00

  • Corneal Endothelium and Phacoemulsification.

    abstract::Phacoemulsification has become the most commonly used procedure in cataract surgery owing to the development of new devices and surgical techniques; however, corneal endothelial damage still represents a serious complication. Although several factors are known to cause corneal endothelial damage, effects of irrigating...


    pub_type: 杂志文章


    authors: Takahashi H

    更新日期:2016-11-01 00:00:00

  • Bilateral epibulbar xanthomas--a case report.

    abstract:PURPOSE:To report bilateral epibulbar xanthomatous lesions in an adult with a normal serum lipid profile and no cutaneous or systemic features of a histiocytic disease. METHODS:Clinicopathologic report. RESULTS:A 44-year-old man presented with gradually enlarging yellow epibulbar masses in both eyes. The lesions had ...


    pub_type: 杂志文章


    authors: Satchi K,Loughnan MS,McKelvie PA,McNab AA

    更新日期:2010-02-01 00:00:00

  • Pyogenic granuloma of the cornea after penetrating keratoplasty.

    abstract::An 81-year-old man with metastatic prostate carcinoma underwent a penetrating keratoplasty for phlyctenular keratitis. Two years later he developed a fleshy, vascular mass in the superotemporal corneal graft wound, at the site of prior graft sutures. An excisional biopsy of the mass was performed to rule out metastati...


    pub_type: 杂志文章


    authors: De Potter P,Tardio DJ,Shields CL,Shields JA

    更新日期:1992-11-01 00:00:00

  • Intraocular lens exchange 1 week after descemet stripping automated endothelial keratoplasty.

    abstract:PURPOSE:To report a case of intraocular lens (IOL) exchange performed 1 week after Descemet stripping automated endothelial keratoplasty (DSAEK). METHODS:Describe the clinical findings and outcome of IOL exchange in an 80-year-old man with pseudophakic bullous keratopathy and retained Artisan (Ophtec BV, Groningen, th...


    pub_type: 杂志文章


    authors: Lapenna L,Kadyan A,Busin M

    更新日期:2010-02-01 00:00:00

  • In vitro susceptibilities of bacterial ocular isolates to fluoroquinolones.

    abstract:PURPOSE:To evaluate and compare the in vitro antimicrobial activity of levofloxacin versus ciprofloxacin and ofloxacin against ocular isolates from patients with bacterial conjunctivitis. METHODS:The in vitro antimicrobial susceptibilities of ocular isolates to levofloxacin, ofloxacin, and ciprofloxacin were determine...


    pub_type: 杂志文章


    authors: Graves A,Henry M,O'Brien TP,Hwang DG,Van Buskirk A,Trousdale MD

    更新日期:2001-04-01 00:00:00

  • Corneal allograft rejection in a pregnant woman.

    abstract::We report a case of corneal allograft rejection that occurred in a gravid woman 1 year following transplantation. This is the first report, to our knowledge, of corneal allograft rejection occurring in a pregnant woman. ...


    pub_type: 杂志文章


    authors: McDonnell PJ,Robin JB

    更新日期:1987-01-01 00:00:00

  • Donor eyes. A comparison of characteristics and outcomes for Eye Bank and local tissue.

    abstract::The Corneal Recipient Registry was begun in 1985 to collect information on all recipients of corneal grafts in the province of Ontario, Canada, and on the donors providing tissue. While most of the tissue is handled by the Eye Bank of Canada (Ontario Division), ophthalmologists in centers away from the Eye Bank often ...


    pub_type: 杂志文章


    authors: Chipman ML,Willett P,Basu PK,Wolf A

    更新日期:1989-01-01 00:00:00

  • Mycobacterium chelonae keratitis: resolution after debridement and presoaked collagen shields.

    abstract::We report a case of Mycobacterium chelonae keratitis following corneal injury by a foreign body. Diagnosis was made by Ziehl-Neelsen staining and Löwenstein-Jensen culture of corneal scrapings. On the basis of the in vitro susceptibility testing, the patient was treated with topical fortified amikacin. Given the lack ...


    pub_type: 杂志文章


    authors: Lois N,Perez del Molino ML

    更新日期:1995-09-01 00:00:00

  • Evaluation of Human Corneal Lenticule Quality After SMILE With Different Cap Thicknesses Using Scanning Electron Microscopy.

    abstract:PURPOSE:To evaluate the surface characteristics of lenticules created by small-incision lenticule extraction (SMILE) with different cap thicknesses. METHODS:This prospective study included 20 consecutive patients who underwent bilateral SMILE. Surface regularity of the extracted corneal lenticule was analyzed using sc...


    pub_type: 杂志文章


    authors: Weng S,Liu M,Yang X,Liu F,Zhou Y,Lin H,Liu Q

    更新日期:2018-01-01 00:00:00

  • Comparison of corneal endothelial image analysis by Konan SP8000 noncontact and Bio-Optics Bambi systems.

    abstract:PURPOSE:Compare corneal endothelial image analysis by Konan SP8000 and Bio-Optics Bambi image-analysis systems. METHODS:Corneal endothelial images from 98 individuals (191 eyes), ranging in age from 4 to 87 years, with a normal slit-lamp examination and no history of ocular trauma, intraocular surgery, or intraocular ...


    pub_type: 杂志文章


    authors: Benetz BA,Diaconu E,Bowlin SJ,Oak SS,Laing RA,Lass JH

    更新日期:1999-01-01 00:00:00