Abstract:
:Native peptides can be regarded as surrogate markers for protease activity in biological samples. Analysis of peptides by peptidomics allows to monitor protease activity in vivo and to describe the influence of protease inhibition. To elucidate the potential of peptides as markers for in vivo protease inhibition we analyzed plasma samples from animals treated with either the indirect FXa inhibitor FONDAPARINUX or the dipeptidylpeptidase IV inhibitor AB192. Signals correlating with the treatment were subsequently identified and assessed with respect to protease-dependent consensus cleavage motifs and occurrence of downstream targets. It could be shown that regulated peptides were either substrates, products or downstream targets of the inhibited protease. The results from the present study demonstrate that the in vivo analysis of peptides by peptidomics has the potential to broaden the knowledge of inhibitor related effects in vivo and that this method may pave the way to develop predictive biomarkers.
journal_name
Peptidesjournal_title
Peptidesauthors
Tammen H,Hess R,Rose H,Wienen W,Jost Mdoi
10.1016/j.peptides.2008.08.016subject
Has Abstractpub_date
2008-12-01 00:00:00pages
2188-95issue
12eissn
0196-9781issn
1873-5169pii
S0196-9781(08)00363-Xjournal_volume
29pub_type
杂志文章相关文献
PEPTIDES文献大全abstract::The presence of alpha-hANP immunoreactive material in human heart and plasma was investigated with a specific and sensitive radioimmunoassay and immunohistochemical method. It was found that alpha-hANP immunoreactive staining of specific atrial granules was located around the nucleus of atrial cardiocytes. No immunore...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(86)90057-4
更新日期:1986-01-01 00:00:00
abstract::Apelin was recently found to be an inotropic polypeptide in isolated rat hearts, and intravenous injection of apelin can induce a transient decrease in blood pressure. To illustrate the mechanism of apelin-induced vasodilation, we observed the in vitro effects of apelin on the L-arginine (L-Arg)/nitric oxide (NO) path...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2007.07.016
更新日期:2007-10-01 00:00:00
abstract::Japonicin-1 (FFPIGVFCKIFKTC) and japonicin-2 (FGLPMLSILPKALCILLKRKC), two peptides with differential growth-inhibitory activity against the Gram-negative bacterium, Escherichia coli and the Gram-positive bacterium Staphylococcus aureus, were isolated from an extract of the skin of the Japanese brown frog Rana japonica...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(01)00634-9
更新日期:2002-03-01 00:00:00
abstract::Cholecystokinin COOH-terminal octapeptide (CCK-8) produces a satiating effect in the rat and other animals upon peripheral administration. Although it has been demonstrated that the receptors which mediate this action are located in the periphery and are of the CCK-A subtype, their anatomical location has not been fir...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(92)90143-q
更新日期:1992-01-01 00:00:00
abstract::The localization of endozepine-like immunoreactivity in the brain of the frog Rana ridibunda was investigated by indirect immunofluorescence, using an antiserum against synthetic rat octadecaneuropeptide (ODN). A specific immunoreaction was detected in ependymal cells lining the ventricular system of the brain and in ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(92)90146-t
更新日期:1992-01-01 00:00:00
abstract::We examined the mechanism through which leptin increases Na(+), K(+)-ATPase activity in the rat kidney. Leptin was infused under anaesthesia into the abdominal aorta proximally to the renal arteries and then Na(+), K(+)-ATPase activity was measured in the renal cortex and medulla. Leptin (1mug/kgmin) increased Na(+), ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2006.08.010
更新日期:2006-12-01 00:00:00
abstract::While neuropeptides of the allatostatin family inhibit in vitro production of juvenile hormone, which modulates aspects of development and reproduction in the cockroach, Diploptera punctata, they are susceptible to inactivation by peptidases in the hemolymph, gut, and bound to internal tissues. Patterns of peptidase c...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(98)00149-1
更新日期:1999-01-01 00:00:00
abstract::Subpopulations of dopamine (DA) neurons in the ventral mesencephalon have been reported to contain cholecystokinin (CCK) and neurotensin (NT), giving rise to DA, DA/NT, NT/CCK and DA/CCK/NT projections. More precisely, colocalized DA/CCK neurons project mainly to the caudal part of the medial nucleus accumbens, wherea...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(92)90073-c
更新日期:1992-05-01 00:00:00
abstract::Studies were conducted in order to determine if selected neurons could be isolated from the brain using Sepharose-linked recognition complexes directed against or related to the biosynthetic/neurosecretory product of the desired neuronal population. Immunoreactive LRF neurons were precipitated when dispersed cells of ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(85)90061-0
更新日期:1985-03-01 00:00:00
abstract::The aim of this study was to compare the actions of CCK neuropeptides within the nucleus accumbens (N.Acc) of alcohol preferring (Fawn-Hooded, FH) and alcohol nonpreferring (Wistar-Kyoto, WKY) rats. CCK-8S (30-300 nM) facilitated the K(+) stimulated release of [(3)H]dopamine (DA) from N.Acc prisms in both rat strains,...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(00)00159-5
更新日期:2000-03-01 00:00:00
abstract::Lysine-8-vasopressin (LVP) was injected into the lateral ventricle (ICV) or into the cisterna magna (c.m.) of ether-anesthetized rats. ICV injection of LVP decreased the blood pressure (BP), whereas c.m. administration increased it. The opposite effects of ICV versus c.m. administration of the peptide might be related...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(86)90021-5
更新日期:1986-05-01 00:00:00
abstract::This work determined Plasmodium falciparum merozoite surface protein-8 (MSP-8) regions specifically binding to membrane surface receptors on human erythrocytes. Five high activity binding peptides (HABPs), whose binding to erythrocytes became saturable and sensitive on being treated with neuraminidase and chymotrypsin...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(03)00185-2
更新日期:2003-07-01 00:00:00
abstract::The synthesis, purification, and characterization of biotinylated analogues of motilin are reported. The C-terminal of canine motilin was extended by the addition of a cysteine residue, and then biotinylated. Biotinyl motilin was purified by following HPLC and characterized by amino acid analysis. Biotinylation of the...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)90011-6
更新日期:1994-01-01 00:00:00
abstract::Previously, we described a gene, flp-1, that encodes seven FMRFamide-like peptides from two alternatively spliced transcripts in the nematode Caenorhabditis elegans. To determine whether all or a subset of the predicted peptides coded for by flp-1 are produced in vivo, we undertook the isolation of FMRFamide-like pept...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90049-m
更新日期:1993-03-01 00:00:00
abstract::The melanocortin 4 receptor (MC4R) plays an essential role in weight regulation. Recently we could show that the MC4R is able to form receptor dimers. In the present study we investigated the role of extracellular cysteine residues and the structure of the third extracellular loop for receptor dimerization. None of th...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.02.027
更新日期:2006-02-01 00:00:00
abstract::Motilin is a regulatory polypeptide of 22 amino acid residues and orginates in motilin cells scattered in the duodenal epithelium of most mammals and chickens. Motilin is released into the general circulation at about 100-min intervals during the interdigestive state and is the most important factor in controlling the...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/s0196-9781(96)00333-6
更新日期:1997-01-01 00:00:00
abstract::Combined modulation of peptide hormone receptors including, glucagon-like peptide-1 (GLP-1), glucose-dependent insulinotropic polypeptide (GIP) and xenin, have established benefits for the treatment of diabetes. The present study has assessed the biological actions and therapeutic efficacy of a novel exendin-4/xenin-8...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2017.10.015
更新日期:2018-02-01 00:00:00
abstract::The effects of dipeptide cyclo-[His-Pro] (CHP), known to participate in the appetite behavior and food intake control, have been investigated using PC12 cells in culture as model system. We found that only in the presence of experimental conditions that cause cellular stress the cyclic dipeptide affect cellular prolif...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.07.010
更新日期:2006-01-01 00:00:00
abstract::Ceratotoxin A (CtxA), a 36-residue alpha-helical cationic peptide isolated from the medfly Ceratitis capitata, exhibits strong antibacterial activity. To determine its mode of action against bacteria, we investigated the behavior of ceratotoxin A by incorporating it into planar lipid bilayers. Macroscopic and single c...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2003.09.015
更新日期:2003-11-01 00:00:00
abstract::This review is part of a special issue dedicated to Opioid addiction, and examines the influential role of opioid peptides, opioid receptors and opiate drugs in mediating food intake and body weight control in rodents. This review postulates that opioid mediation of food intake was an example of "positive addictive" p...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2019.04.008
更新日期:2019-06-01 00:00:00
abstract::Obestatin is purported to be a peptide hormone encoded in preproghrelin. We studied the metabolic effects of continuous infusion of obestatin via subcutaneously implanted osmotic mini-pumps. Administration of up to 500nmol/kg body weight/day obestatin did not change 24h cumulative food intake or body weight in rats. S...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2008.03.023
更新日期:2008-08-01 00:00:00
abstract::To study the effects of inducing stereo-chemical modifications in the structure of dermorphin (DM) so as to improve its mu-opioid receptor affinity and its resistance to C-terminal enzymatic degradation, in the Institute of Molecular Genetics of Moscow, we synthesized a new DM analogue ([DPro(6)]DM) and analyzed the c...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(03)00057-3
更新日期:2003-03-01 00:00:00
abstract::Antimicrobial peptides (AMPs) and mucins are components of airway secretions and both contribute to the innate host defense system. At neutral pH, AMPs are positively charged, mucins negatively. It was the aim of the study to test whether these opposite charges result in interactions between AMPs and mucins. We measur...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2006.07.018
更新日期:2006-12-01 00:00:00
abstract::We investigated the ability of sulfated cholecystokinin (26-33) (CCK-8) and cholecystokinin (30-33) (CCK-4) to induce taste aversion or avoidance conditioning (TAC) in a one-bottle testing paradigm after either intravenous (i.v.), intracerebroventricular (i.c.v.), or intraperitoneal (i.p.) administration. Significant ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(98)00025-4
更新日期:1998-01-01 00:00:00
abstract::Pituitary Adenylate-Cyclase Activating Polypeptide (PACAP) and Tac1 gene-encoded tachykinins (substance P: SP, neurokinin A: NKA) are expressed in capsaicin-sensitive nerves, but their role in nociception, inflammation and vasoregulation is unclear. Therefore, we investigated the function of these neuropeptides and th...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2013.03.003
更新日期:2013-05-01 00:00:00
abstract::For clinical use, it is essential to develop potent endomorphin (EM) analogs with reduced antinociceptive tolerance. In the present study, the antinociceptive activities and tolerance development of four potent EM-1 analogs with C-terminal oligoarginine-conjugation was evaluated and compared in the radiant heat paw wi...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2018.07.004
更新日期:2018-08-01 00:00:00
abstract::Deltorphin, a heptapeptide that selectively binds to delta receptors, was intracerebroventricularly administered to mice belonging to the strains C57BL/6 and DBA/2 immediately after training in a one-trial inhibitory avoidance task. The retention performance of both strains was improved by the peptide administration, ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(90)90063-b
更新日期:1990-05-01 00:00:00
abstract::Higher levels of natriuretic peptides were identified in outpatients after myocardial infarction (MI) compared to the healthy population, even in the absence of heart failure (HF). Therefore, we assessed the optimal cut-off value of B-type natriuretic peptide (BNP) in relation to new-onset HF prediction in 79 post-MI ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.06.023
更新日期:2010-10-01 00:00:00
abstract::Obesity is a chronic inflammatory disease significantly risen worldwide, especially among children. Adipokines, secreted from adipose tissue, are hormones involved in various cellular processes such as energy metabolism and inflammation. Among the others, adiponectin is gaining increasing interest for its insulin-sent...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2014.11.007
更新日期:2015-01-01 00:00:00
abstract::Heat-stable enterotoxin b (STb) of Escherichia coli is a 48-amino acid basic, disulfide-bonded peptide that causes intestinal secretion in experimental animal models. Recent evidence suggests that the in vivo mechanism of STb action involves release of 5-hydroxytryptamine (5-HT) and production of prostaglandin E2 (PGE...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(96)00033-2
更新日期:1996-01-01 00:00:00