Abstract:
:Covalent binding of poly(ethylene glycol), abbreviated as PEG, to red blood cells (RBC) surface leads to masking of the RBC blood group determinants and the PEG layer on the cell surface sterically hinders RBC-RBC and RBC-plasma protein interactions. We cross-linked linear mPEG-SPA of various molecular mass (2000, 5000, 20000) to washed human RBC under varying incubation ratios polymer to RBC. The electrophoretic mobility (EM) of the modified RBC decreases with increasing of chain length and concentration of PEG up to 50%. It may reflect the alteration in the surface layer thickness and friction. The aggregation behaviour of the pegylated RBC was studied with the Zeta sedimentation technique modifying the cell-cell interactions pressing them toward each other under centrifugal forces of various magnitudes. As a rule at low centrifugation forces the increase in chain length and concentration of PEG linked to RBC surface reduces the dextran-induced aggregation probably via elevation of the steric repulsion, which counteracts the depletion force generated by the free polymer. This effect was reversed to some extent by elevation of free dextran concentration and centrifugation forces. If cell-cell polymer bridging starts playing a role under these conditions requires further experimental and theoretical investigations.
journal_name
Clin Hemorheol Microcircjournal_title
Clinical hemorheology and microcirculationauthors
Jovtchev S,Stoeff S,Arnold K,Zschörnig Osubject
Has Abstractpub_date
2008-01-01 00:00:00pages
229-33issue
1-4eissn
1386-0291issn
1875-8622journal_volume
39pub_type
杂志文章abstract::Beta-thalassaemia is a hereditary hemolytic disease, in which each clinic phenotype encompasses a heterogenic group of genetic alterations resulting in beta-globin chain synthesis decrease or absence in red blood cells. Studies on beta-thalassaemia carriers suggest the existence of decreased red cell deformability. Th...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::The neurologic outcome of 23 seven-year old children who had cord blood hyperviscosity was compared with that of children with normal cord blood viscosity in a randomised, controlled and blinded study. Viscosity was measured using a coaxial narrow-gap couette viscometer. Sixteen (69.6%) of the children with hypervisco...
journal_title:Clinical hemorheology and microcirculation
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Cardiac surgery with cardiopulmonary bypass is associated with important changes in the microcirculation, usually attributed to endothelial dysfunction. Another common finding of cardiac surgery is postoperative thrombocytopenia and platelet loss of function. OBJECTIVE:To investigate the association between...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-180391
更新日期:2018-01-01 00:00:00
abstract::Hypothyroidism is associated with atherosclerotic events, however, the mechanism is unclear. We investigated the effects of oxidative stress and cholesterol on plasma viscosity in female patients with hypothyroidism (n = 20; mean age: 45.5 +/- 5.5 years) at baseline and after L-thyroxine replacement therapy (average d...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract:PURPOSE:To compare the value of high-resolution contrast-enhanced ultrasound in the detection and characterisation of endoleaks after EVAR. MATERIAL AND METHODS:In this retrospective study, a cohort of 80 patients underwent both standard examination using a curved array 5 MHz transducer and a second examination using ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-141896
更新日期:2014-01-01 00:00:00
abstract::It was investigated whether the NO-donor SIN-1, the active metabolite of molsidomine, influenced the activation of platelets, the formation of circulating platelet aggregates, the spontaneous aggregation of platelets and the activation of the clotting system triggered by a body foreign surface in an in vitro closed-lo...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:Hemorheologic factors such as red blood cell (RBC) aggregation and deformability differ according to species. In many comparative hemorheologic studies, only venous blood samples have been used for measurements. There is little published information comparing arterial and venous blood differences between spe...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2010-1248
更新日期:2010-01-01 00:00:00
abstract::To compare the level of endothelial nitric oxide synthase (eNOS) expression produced in heart and lung vascular tissue, the protein content was determined using Western blot analysis with the enhancement of image processing. Heart and lung extracts from 12 and 24 weeks from control (CON) and streptozotocin-induced dia...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Complications of cocaine administration are acute vascular occlusions such as myocardial infarction and stroke. We have studied the influence of cocaine on platelet function in vitro. For that purpose, citrated blood from healthy volunteers was incubated with cocaine concentrations of 0 (control), 10, 100, 1000, 2500,...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-131795
更新日期:2014-01-01 00:00:00
abstract::The present study was to investigate the protective effects of salvianolic acids (SA) on deformability of red blood cells (RBCs) and its mechanism during the development of acute lung injury (ALI) induced by oleic acid (OA) in rabbits. 32 rabbits were randomized into four groups, normal control group, OA-treated group...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::The aim of this study was to assess social and health status of residents older than 90 years in an East-Hungarian city. The investigation involved 70 subjects, measuring routine laboratory parameters and plasma viscosity. Mean cholesterol, erythrocyte sedimentation rate (ESR), collagen cross links and plasma viscosit...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::The involvement of plasma nitric oxide metabolites (NO(x)) in hypertension was examined in stroke-resistant spontaneously hypertensive rats during the development of hypertension. Continuous application of a static magnetic field (SMF; a maximum magnetic flux density of 180 millitesla, a peak magnetic gradient of 133 ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:OBJECTIVE:To investigate macro- and microperfusion during 14 days of treatment with a new 2-layer compression system (3M™ Coban™ 2 Lite), designed for patients with leg ulcer and concomitant peripheral arterial occlusive disease. METHODS:A single-centre, open-label, prospective pilot study was performed with 15 subjec...
journal_title:Clinical hemorheology and microcirculation
pub_type: 临床试验,杂志文章
doi:10.3233/CH-2012-1568
更新日期:2013-01-01 00:00:00
abstract::The lipoprotein lipase deficient (LPL-/-) mice and high fat-diet (HFD) induced hypertriglyceridemic mice were used to investigate the effects of cardiotonic pill (CP) on RBC rheologic abnormalities. Mice were randomly divided into the following five groups: the control group; the untreated HFD group; the untreated LPL...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Carotid stenting stimulates intimal proliferation through platelet and stem cell activation. OBJECTIVE:The aim of this study is to evaluate whether the administration before or after carotid stenting of clopidogrel loading dose may play a role on circulating endothelial progenitor cells, stromal cell-derive...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章,随机对照试验
doi:10.3233/CH-180429
更新日期:2019-01-01 00:00:00
abstract:AIM:Use of contrast enhanced ultrasound (CEUS) in severe cases of COVID-19 infection to assess pulmonary changes near the pleura. MATERIAL AND METHODS:Bedside examinations by an experienced intensive care unit examiner using a multi-frequency probe (C1-6 MHz) with B-mode and CEUS to assess pleural-near changes in seve...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-209005
更新日期:2020-01-01 00:00:00
abstract::The source and target of edema fluid for ischemic brain swelling clinically often observed in "malignant infarction" was examined in ex vivo. Wister rat brain hemispheres were removed and incubated air-tightly in a deoxygenated artificial cerebrospinal fluid at 37 degrees for 30 min. Ionic movement into the brain tiss...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:PURPOSE:The purpose of this study was to evaluate whether image fusion with contrast enhanced ultrasound (CEUS) and CT is effective in the pre-, intra- and post-interventional management of liver lesions during microwave or radiofrequency ablation. METHODS AND MATERIALS:Fifteen patients with a single hepatocellular ca...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2012-1598
更新日期:2012-01-01 00:00:00
abstract::Excessive time for analysis may impede microcirculatory studies with large amounts of video data. Engaging more personnel in the analyses seems to be a rational approach in that scenario and could shorten the time-interval between capturing images and obtaining results. Our hypothesis was that novice users would be ab...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章,多中心研究
doi:10.3233/CH-151958
更新日期:2016-01-01 00:00:00
abstract::Recently, it was shown that mesenchymal stem cells (MSCs) are capable of differentiating into endothelial cells which highlights the potential role of MSCs in neovascularization. In the present study, we investigated the paracrine factors responsible for tube formation in human adipose-tissue derived stem cells (ASCs)...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2011-1397
更新日期:2011-01-01 00:00:00
abstract::The main functions of the blood are the transport, and delivery of oxygen and nutrients, removal of carbon dioxide and waste products of metabolism, distribution of heat and signals of immune system. They are provided by circulation due to the driving force of the heart. Circulation of the blood depends on its rheolog...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章,评审
doi:10.3233/CH-2009-1192
更新日期:2009-01-01 00:00:00
abstract:AIM:To investigate three-year outcomes of patients presenting with nonSTelevation acute coronary syndrome (NSTE-ACS) treated with bioresorbable everolimus-eluting vascular scaffolds (BRS). METHODS AND RESULTS:The study included a total of 488 consecutive patients, enrolled between May 2012 and December 2013 (median ag...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-189101
更新日期:2018-01-01 00:00:00
abstract::In the present study, the role of the nucleus and its contribution to the deformability of the passive neutrophils was investigated. To determine the rheological properties of the nucleus and of the neutrophil itself, deformation tests on single neutrophil and sequestered nucleus have been performed by micropipette un...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2010-1326
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVE:To assess sex-related differences in forearm skin microvascular reactivity to vascular occlusion and cardiovascular health in young healthy women and men. METHODS:203 young healthy individuals (67 men/136 women) participated in this study. Post-occlusive reactive hyperemia (PORH) in skin microcirculation fol...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-180483
更新日期:2019-01-01 00:00:00
abstract::Altitude training is frequently used by athletes to improve sea-level performance. However, the objective benefits of altitude training are controversial. This study aimed to investigate the possible alterations in hemorheological parameters in response to altitude training. Sprague Dawley rats, were divided into 6 gr...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-131711
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:IL-17 is considered to be a cancer-promoting gene in hepatocellular carcinoma (HCC). Here, we explored the effect of IL-17 in predicting the therapeutic effect of transcatheter arterial chemoembolization (TACE) combined with apartinib in patients with HCC in this study. METHODS:Established of IL-17 knockdown...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-200857
更新日期:2020-07-09 00:00:00
abstract:BACKGROUND:Advances in rapid-prototyping and 3D printing technologies have enhanced the possibilities in preparing designed architectures for tissue engineering applications. A major advantage in custom designing is the ability to create structures with desired mechanical properties. While the behaviour of a designed s...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-151931
更新日期:2015-01-01 00:00:00
abstract::In the vascular system, the shear applied to the vascular wall activates mechano-sensors located on endothelial cells (ECs) leading to a modification in the gene expression profile. We applied laminar shear stress at 1 Pa on ECs for 6 h and measured by quantitative real time PCR the expression modulation of genes impl...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::In order to ascertain whether young asymptomatic patients with heterozygous familial hypercholesterolemia (FH) but without clinical signs of atherosclerosis have increased plasma thrombomodulin (TM) levels expressing early endothelial damage, we determined TM in 23 heterozygous FH subjects (aged 28-44 years, x: 32 +/-...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:Glaucoma is currently attributed to two different possible pathogenetic mechanisms: mechanical (the ocular damage is induced by physical injury), and vascular (the ocular damage is sustained by ischemia of the optic nerve head). AIM:We considered the possibility that several anti-glaucoma drugs (beta-blocke...
journal_title:Clinical hemorheology and microcirculation
pub_type: 临床试验,杂志文章
doi:
更新日期:2006-01-01 00:00:00