Abstract:
:Recently, it was shown that mesenchymal stem cells (MSCs) are capable of differentiating into endothelial cells which highlights the potential role of MSCs in neovascularization. In the present study, we investigated the paracrine factors responsible for tube formation in human adipose-tissue derived stem cells (ASCs). Moreover, we analyzed ASC's migration towards PDGF-BB and altered levels of proteins involved in different pathways. Freshly isolated human adipose tissue-derived stem cells were seeded onto wells coated with Matrigel and cultured in endothelial growth medium. Capillary-like tube formation was observed after 18 hours culture. Tube formation was significantly reduced in the presence of antibodies against platelet-derived growth factor receptor beta (PDGF) or basic fibroblast growth factor (bFGF). Reverse phase proteomic assay (RPPA) was used to interrogate the expression of 139 phosphorylated or native proteins after incubation with PDGF-BB protein for 24 hours. The present data suggest, that freshly isolated ASCs contain a subpopuplation of stem cells that can form capillary like tubes which is dependent on PDGF and bFGF signaling pathway. Furthermore, Migration of human ASCs significantly increased in response to increased concentrations of PDGF-BB. In addition, incubation of ASCs with PDGF-BB altered phosphorylation of several transcription proteins that are widely expressed throughout the hematopoietic system, targeting genes that have been associated with proliferation, anti-apoptosis or differentiation.
journal_name
Clin Hemorheol Microcircjournal_title
Clinical hemorheology and microcirculationauthors
Gehmert S,Gehmert S,Hidayat M,Sultan M,Berner A,Klein S,Zellner J,Müller M,Prantl Ldoi
10.3233/CH-2011-1397subject
Has Abstractpub_date
2011-01-01 00:00:00pages
5-13issue
1eissn
1386-0291issn
1875-8622pii
40212P27620705V5journal_volume
48pub_type
杂志文章abstract:INTRODUCTION:The hormones erythropoietin (EPO) and thrombopoietin (TPO) are main regulators of erythro- and thrombopoiesis. Cell loss caused by operative procedures may alter serum levels of the hormones, resulting in well known phenomenons like reactice thrombocytosis. MATERIAL AND METHODS:Blood samples from 10 patie...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2011-1489
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVE:To identify the indications for CEUS in renal imaging in an interdisciplinary ultrasound department. METHODS:102 CEUS examinations of the kidney in 82 patients between September 2014 and July 2016 were analysed regarding the indication for ultrasound. CEUS was performed by one experienced sonographer agent a...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-179103
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Renal ischemia-reperfusion injury (IRI) is common in renal transplantation, shock, and nephrolithotomy. OBJECTIVE:To quantitatively assess rabbit renal IRI with contrast-enhanced ultrasound (CEUS) and investigate associations between renal cortical time-intensity curve (TIC) parameters and intracellular adh...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-131768
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Capsular contractures around breast implants usually develop leading to pain and aesthetically inadequate results and ultimately often requires the replacement of the implants. Textured silicone implants are the most commonly placed implant, but polyurethane-coated implants are increasingly being used in an ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-200875
更新日期:2020-09-08 00:00:00
abstract::We described a patient with symptomatic giant hepatic hemangioma (GHH) treated with laparoscopic guided percutaneous microwave ablation (MWA). A 58 years' old woman was referred to our hospital who presented with upper abdominal distension and appetite loss for more than 1 year. The medical history included untreated ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-200922
更新日期:2020-10-13 00:00:00
abstract::Radiofrequency ablation (RFA) has shown to be a reasonable alternative for the treatment of hepatic tumors and metastases although multiple limitations remain. Cooling effects due to larger vessels can prevent complete coverage and may lead to early tumor relapse. This preliminary in vivo pig study combines the use of...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-141886
更新日期:2014-01-01 00:00:00
abstract::Hypercoagulable states due either to inherited or acquired thrombotic risk factors are only present in approximately half of cases of DVT, but the causes in the other half, remain unknown. The importance of biological risk factors such as hyperlipidemia, hypofibrinolysis and hemorheological alterations in the pathogen...
journal_title:Clinical hemorheology and microcirculation
pub_type:
doi:
更新日期:2002-01-01 00:00:00
abstract:INTRODUCTION:Laparoscopy is more beneficial than the conventional open technique, however the pneumoperitoneum created may have an ischemic side effect. OBJECTIVE:Our aim was to evaluate the protective effects of preconditioning during laparoscopic cholecystectomies (LC). METHODS:30 patients were randomized into 2 gr...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-170336
更新日期:2018-01-01 00:00:00
abstract::Anemia in pregnant women is associated with increased maternal and perinatal mortality and represents an important economic burden in many developing countries. Our goal was to evaluate the impact of anemia on the capillary network during pregnancy. Therefore, we compared microcirculatory parameters of anemic pregnant...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-162064
更新日期:2016-11-25 00:00:00
abstract::In Chinese traditional medicine, angelica is widely used for its known clinical effects of ameliorating blood microcirculation. But the mechanism of these beneficial effects still remains unclear. In this work the rheological behaviour of human erythrocytes treated by angelica was studied in vitro. Normal RBCs incubat...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::It is well established that exercise performance in athletes is related to improved blood fluidity. However, training effects on functional state of endothelium and relations of endothelial cell functions with hemorheology are poorly known. Circulating levels of von Willebrand factor may serve as a good marker of endo...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:PURPOSE:To explore the peripapillary capillary density distribution and its correlation with nerve fibre layer in superficial and deep retinal layers of healthy Chinese. METHODS:Images of superficial and deep retinal capillary networks were obtained by optical coherence tomography angiography (OCTA) using a CIRRUS TM ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-180453
更新日期:2020-01-01 00:00:00
abstract::In this study, the human RBC capillary flow has been modeled by passing 11 microl of RBC suspension (Hematocrit = 6%) in phosphate buffer solution (PBS) of a viscosity of 1 and 2.6 cP (in the presence of 2% Dextran) through 5 microm pore diameter polycarbonate Nuclepore filters. We have developed a digitally controlle...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2009-1242
更新日期:2009-01-01 00:00:00
abstract::Vascular endothelial cells form a monocellular layer on blood vessel walls with an estimated mass of 1.5 kg. One of the roles of endothelial cells is to control the hemodynamics through various metabolic activities affecting homeostasis, vascular tonus, blood fluidity, coagulating properties and blood cell adhesion. I...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVE:Urea transporter-B (UT-B), highly expressed in erythrocyte, confers specific permeability to urea, urea analogues and water. The purpose of this study was to determine the hemorheological properties of UT-B null erythrocyte using a series of biophysical techniques. METHODS AND RESULTS:The blood was taken fro...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-16174
更新日期:2017-01-01 00:00:00
abstract::The main functions of the blood are the transport, and delivery of oxygen and nutrients, removal of carbon dioxide and waste products of metabolism, distribution of heat and signals of immune system. They are provided by circulation due to the driving force of the heart. Circulation of the blood depends on its rheolog...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章,评审
doi:10.3233/CH-2009-1192
更新日期:2009-01-01 00:00:00
abstract::It is now clear that different pathophysiologic mechanisms have a profound influence on the extent of the functional impairment in intermittent claudication. In particular, metabolic derangements, including impaired oxygen delivery and/or extraction, reduced nitric oxide synthesis, reduced glucose oxidation, accumulat...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章,评审
doi:
更新日期:1999-01-01 00:00:00
abstract::Erythrocyte deformability could be a suitable parameter reflecting hyposplenic-asplenic conditions after splenectomy or spleen autotransplantation. The aim of this study was to analyze the efficacy of spleen autotransplantation using filtrometry and ektacytometry for testing erythrocyte deformability, besides complex ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2010-1307
更新日期:2010-01-01 00:00:00
abstract::There are few studies on haemorheological disturbances in morbidly obese patients. The role played by the metabolic syndrome on the rheological profile of morbidly obese subjects has not yet been established, and it is not clear whether morbidly obese, but "metabolically healthy", show rheological alterations. We aime...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2011-1417
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND AND AIM:Hematocrit is an important hemorheological parameter. Both hematocrit and nonalcoholic fatty liver disease (NAFLD) are strongly correlated with cardiovascular disease. However, there is no study to explore the direct relationship of hematocrit with NAFLD. METHODS:Hematocrit levels were analyzed from...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2011-1509
更新日期:2012-01-01 00:00:00
abstract::In a group of young subjects with acute myocardial infarction (AMI) (97 men and 8 women; mean age 39.6+/-5.5 years) we examined the thiobarbituric acid - reactive substances and the total antioxidant status at the initial stage and after 12 months. The same parameters were examined in a group of 55 control subjects. O...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::Abnormalities in labyrinth vasculature, resulting in labyrinth ischemia may be responsible for acute unilateral vestibular syndrome (AVS). However, since no tools for the study of the labyrinth microvasculature are available in clinical settings, labyrinth microvascular abnormalities in AVS patients (AVS-pts) can only...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2012-1554
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:We have studied dense erythrocytes separated on Arabinogalactan (Stractan) ultracentrifuged gradients in flame-burned patients and in normal individuals. In each case, the percentage of erythrocytes in the densest layers was increased when compared to age and sex matched controls. METHODS AND RESULTS:Using a...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2012-1556
更新日期:2013-01-01 00:00:00
abstract::Although there are several studies dealing with erythrocyte deformability (ED) in obese patients, research on this topic in morbidly obese subjects is scarce. In these studies ED seems to be decreased, although the cause remains unknown. A case-control study in 76 morbid obese subjects (23 women and 53 men, aged 44 ± ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2010-1357
更新日期:2010-01-01 00:00:00
abstract::Ektacytometry is frequently used to study red blood cell (RBC) deformability. This method is capable of measuring RBC deformation under a wide range of shear stresses, and the results are usually presented as shear stress-elongation index curves. These curves are very useful for detailed analyses of RBC mechanical beh...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Renal failure is a disease with accelerated atherosclerosis beginning with endothelial cell dysfunction. Factors affecting endothelial cell dysfunction include whole blood viscosity (WBV) and asymmetric dimethylarginine (ADMA). The relationship in controls and renal failure was determined. METHODS:51 subjec...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-141843
更新日期:2015-01-01 00:00:00
abstract::We report the case of a 61-year-old patient admitted to our hospital owing to recent nonspecific abdominal pain. Five years earlier he had been treated with a graft owing to thoracic and abdominal aortic aneurysm with dissection. He now showed a left renal vein aneurysm with a diameter of 11x7.5 cm. This case demonstr...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::Determination of shear stresses at given shear rates allow approximation of flow curves by mathematical models and to calculate viscosities of non-Newtonian fluids. In term neonates, the mean arterial blood pressure (MAP) is markedly below that of adults, therefore rheological properties of blood play an important rol...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-152008
更新日期:2015-10-06 00:00:00
abstract::Under both the in vivo and in vitro conditions we investigated the insulin effect on the most significant factor disturbing the blood rheological disorders in the microcirculation, the red blood cell aggregability. The in vivo studies we carried out in the 42 insulin treated diabetic patients (diabetes mellitus type I...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::The erythrocyte adenosine triphosphate (ATP) is utilised for protein phosphorylation and exported through the pannexin 1 hemichannel (Px1) in the microcirculation. The physiological stimuli for ATP release are dependent of blood shear rate level and of the tissue oxygen content. The deoxygenated and oxygenated states ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-141833
更新日期:2015-01-01 00:00:00