当前位置： SCI文献检索 > JOURNAL OF MOLECULAR ENDOCRINOLOGY期刊下所有文献 > Expression of a hyperactive androgen receptor leads to androgen-independent growth of prostate cancer cells.

Expression of a hyperactive androgen receptor leads to androgen-independent growth of prostate cancer cells.

Abstract：

:Cellular changes that affect the androgen receptor (AR) can cause prostate cancer to transition from androgen dependent to androgen independent, which is usually lethal. One common change in prostate tumors is overexpression of the AR, which has been shown to lead to androgen-independent growth of prostate cancer cells. This led us to hypothesize that expression of a hyperactive AR would be sufficient for androgen-independent growth of prostate cancer cells. To test this hypothesis, stable lune cancer prostate (LNCaP) cell lines were generated, which express a virion phosphoprotein (VP)16-AR hybrid protein that contains full-length AR fused to the strong viral transcriptional activation domain VP16. This fusion protein elicited as much as a 20-fold stronger transcriptional activity than the natural AR. Stable expression of VP16-AR in LNCaP cells yielded androgen-independent cell proliferation, while under the same growth conditions the parental LNCaP cells exhibited only androgen-dependent growth. These results show that expression of a hyperactive AR is sufficient for androgen-independent growth of prostate cancer cells. To study the molecular basis of this enhanced growth, we measured the expression of soluble guanylyl cyclase-alpha1 (sGCalpha1), a subunit of the sGC, an androgen-regulated gene that has been shown to be involved in prostate cancer cell growth. Interestingly, the expression of sGCalpha1 is androgen independent in VP16-AR-expressing cells, in contrast to its androgen-induced expression in control LNCaP cells. RNA(I)-dependent inhibition of sGCalpha1 expression resulted in significantly reduced proliferation of VP16-AR cells, implicating an important role for sGCalpha1 in the androgen-independent growth of these cells.

journal_name

J Mol Endocrinol

journal_title

Journal of molecular endocrinology

authors

Hsieh CL,Cai C,Giwa A,Bivins A,Chen SY,Sabry D,Govardhan K,Shemshedini L

doi

10.1677/JME-07-0158

subject

Has Abstract

pub_date

2008-07-01 00:00:00

pages

13-23

issue

1

eissn

0952-5041

issn

1479-6813

pii

JME-07-0158

journal_volume

41

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR ENDOCRINOLOGY文献大全
  • Corticotropin-releasing factor and its receptors in the brain of rats with insulin and corticosterone deficits.

    abstract：:The expression of genes encoding corticotropin-releasing factor (CRF) and its receptor type-1 (CRF1R) and type-2alpha (CRF2R) has been studied in the brain of rats with streptozotocin (STZ)-induced diabetes and adrenalectomy (ADX). Diabetic rats had a lower body weight compared to control rats. Food and water intake w...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.1.02103

    authors： Huang Q,Timofeeva E,Richard D

    更新日期：2006-10-01 00:00:00

  • Detection of retinoic acid receptor mRNA in rat tissues by reverse transcriptase-polymerase chain reaction.

    abstract：:The presence of retinoic acid receptor (RAR) alpha, beta and gamma mRNA was examined in 16 different kinds of rat tissue using the highly sensitive reverse transcriptase-polymerase chain reaction technique. The data demonstrated that each tissue expressed at least two types of RAR mRNA. Among the three types of RAR mR...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0090291

    authors： Wan YJ,Wang L,Wu TC

    更新日期：1992-12-01 00:00:00

  • Androgen-mediated improvement of body composition and muscle function involves a novel early transcriptional program including IGF1, mechano growth factor, and induction of {beta}-catenin.

    abstract：:Androgens promote anabolism in the musculoskeletal system while generally repressing adiposity, leading to lean body composition. Circulating androgens decline with age, contributing to frailty, osteoporosis, and obesity; however, the mechanisms by which androgens modulate body composition are largely unknown. Here, w...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/JME-09-0048

    authors： Gentile MA,Nantermet PV,Vogel RL,Phillips R,Holder D,Hodor P,Cheng C,Dai H,Freedman LP,Ray WJ

    更新日期：2010-01-01 00:00:00

  • Effects of sex steroids on gonadotropin (FSH and LH) regulation in coho salmon (Oncorhynchus kisutch).

    abstract：:The effect of steroid hormone treatment on coho salmon (Oncorhynchus kisutch) was examined. The cDNAs for coho salmon FSH beta and LH beta subunits were cloned and sequenced using reverse transcriptase PCR. Northern blot analysis revealed that a single transcript of 1 kb for each of these subunits was present in the p...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0210291

    authors： Dickey JT,Swanson P

    更新日期：1998-12-01 00:00:00

  • Molecular characterization and in-vitro hormonal requirements for expression of two casein genes from a marsupial.

    abstract：:Two marsupial casein genes have been isolated from a tammar wallaby (Macropus eugenii) mammary gland cDNA library. Comparisons of the tammar alpha- and beta-casein genes with their eutherian homologous reveal extensive divergence at the levels of nucleotide and amino acid sequences. Regions of similarity between the t...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0080013

    authors： Collet C,Joseph R,Nicholas K

    更新日期：1992-02-01 00:00:00

  • A luciferase transgenic mouse model: visualization of prostate development and its androgen responsiveness in live animals.

    abstract：:Numerous mouse models of prostate carcinogenesis have been developed, but hitherto there has been no model in which the prostate gland could be imaged in live animals. The transgenic model generated here targeted mouse prostate gland using a firefly luciferase enzyme under the control of a small but highly active and ...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.1.01722

    authors： Hsieh CL,Xie Z,Liu ZY,Green JE,Martin WD,Datta MW,Yeung F,Pan D,Chung LW

    更新日期：2005-10-01 00:00:00

  • Effects of essential amino acids on lipid metabolism in mice and humans.

    abstract：:Eight amino acids are considered essential for human nutrition, and three of them, including leucine, isoleucine and valine, are called as branched-chain amino acids (BCAAs). We recently discovered that dietary deficiency of any BCAA for 7 days rapidly reduces the abdominal fat mass in mice. The goal of this study was...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1530/JME-16-0116

    authors： Xiao F,Du Y,Lv Z,Chen S,Zhu J,Sheng H,Guo F

    更新日期：2016-11-01 00:00:00

  • Protein kinase C and the regulation of insulin secretion from pancreatic B cells.

    abstract：:Protein kinase C (PKC) has been identified in islets of Langerhans and insulin-secreting tumour cells. Diacylglycerols (DAGs, the endogenous PKC activators) are generated in response to insulin secretagogues, although nutrient and non-nutrient secretagogues generate DAGs of different compositions and of different pote...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1677/jme.0.0060121

    authors： Jones PM,Persaud SJ,Howell SL

    更新日期：1991-04-01 00:00:00

  • Insulin-like growth factor (IGF)-I A- and B-domain analogues with altered type 1 IGF and insulin receptor binding specificities.

    abstract：:Insulin-like growth factor-I (IGF-I) analogues were produced with the aim of identifying IGF-I residues that contribute to the specificity of binding to the type 1 IGF receptor as opposed to the insulin receptor. Receptor binding properties of a series of A- and B-domain analogues were compared using rat L6 myoblasts,...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0170237

    authors： Shooter GK,Magee B,Soos MA,Francis GL,Siddle K,Wallace JC

    更新日期：1996-12-01 00:00:00

  • Cerebral and gonadal aromatase expressions are differently affected during sex differentiation of Pleurodeles waltl.

    abstract：:In vertebrates, sex is determined essentially by two means, genetic factors located on sex chromosomes and epigenetic factors such as temperature experienced by the individual during development. Steroids, especially estrogens, are clearly involved in gonadal differentiation in non-mammalian vertebrates. In this regar...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.1.01588

    authors： Kuntz S,Chesnel A,Flament S,Chardard D

    更新日期：2004-12-01 00:00:00

  • Identification and cloning of a glycoprotein hormone receptor from sea lamprey, Petromyzon marinus.

    abstract：:A full-length transcript encoding a functional lamprey glycoprotein hormone receptor I (lGpH-R I, GenBank AY750688) was cloned from the testes of the sea lamprey, Petromyzon marinus, using the GpH-R protein fingerprint GLYCHORMONER from the PRINTS database. The present study is the first to identify a GpH-R transcript...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.1.02064

    authors： Freamat M,Kawauchi H,Nozaki M,Sower SA

    更新日期：2006-08-01 00:00:00

  • Cloning and characterization of cDNAs encoding steroidogenic acute regulatory protein from freshwater stingrays (Potamotrygon spp.).

    abstract：:The steroidogenic acute regulatory protein (StAR) is critical to the regulated synthesis of steroids in vertebrates. We have isolated cDNA sequences encoding StAR in the freshwater stingrays Potamotrygon hystrix and P. motoro. A single P. hystrix StAR transcript (3376 bp) and two overlapping P. motoro StAR transcripts...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.1.01857

    authors： Nunez BS,Piermarini PM,Evans AN,Applebaum SL

    更新日期：2005-12-01 00:00:00

  • Homologous and heterologous in vitro regulation of pig pituitary somatostatin receptor subtypes, sst1, sst2 and sst5 mRNA.

    abstract：:Somatostatin (SRIF) is commonly regarded as an inhibitor of GH release in rodents and humans. However, in pigs, SRIF can stimulate the release of GH at low (picomolar) doses, while inhibiting GHRH-stimulated GH release at high (nanomolar) doses in primary pituitary cell cultures. One possible mechanism by which pig ce...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0320437

    authors： Luque RM,Park S,Peng XD,Delgado E,Gracia-Navarro F,Kineman RD,Malagón MM,Castaño JP

    更新日期：2004-04-01 00:00:00

  • Hypochlorous acid via peroxynitrite activates protein kinase Cθ and insulin resistance in adipocytes.

    abstract：:We recently reported that genetic deletion of myeloperoxidase (MPO) alleviates obesity-related insulin resistance in mice in vivo. How MPO impairs insulin sensitivity in adipocytes is poorly characterized. As hypochlorous acid (HOCl) is a principal oxidant product generated by MPO, we evaluated the effects of HOCl on ...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1530/JME-14-0213

    authors： Zhou J,Wang Q,Ding Y,Zou MH

    更新日期：2015-02-01 00:00:00

  • Thyroid hormone regulation of prohormone convertase 1 (PC1): regional expression in rat brain and in vitro characterization of negative thyroid hormone response elements.

    abstract：:Most pro-neuropeptides are processed by the prohormone convertases, PC1 and PC2. We previously reported that changes in thyroid status altered anterior pituitary PC1 mRNA and this regulation was due to triiodothyronine (T(3))-dependent interaction of thyroid hormone receptor (TR) with negative thyroid hormone response...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0330021

    authors： Shen X,Li QL,Brent GA,Friedman TC

    更新日期：2004-08-01 00:00:00

  • The -514 C/T polymorphism in the hepatic lipase gene promoter is associated with insulin sensitivity in a healthy young population.

    abstract：:Impaired insulin action has been associated with diabetes, dyslipidemia and atherosclerotic vascular disease. The expression of insulin resistance results from the interaction of environmental and genetic factors. Human hepatic lipase (HL) is a lipolytic enzyme that plays a role in the metabolism of several lipoprotei...

    journal_title：Journal of molecular endocrinology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1677/jme.1.01627

    authors： Gómez P,Pérez-Jiménez F,Marín C,Moreno JA,Gómez MJ,Bellido C,Pérez-Martínez P,Fuentes F,Paniagua JA,López-Miranda J

    更新日期：2005-04-01 00:00:00

  • A role for PFKFB3/iPFK2 in metformin suppression of adipocyte inflammatory responses.

    abstract：:Metformin improves obesity-associated metabolic dysregulation, but has controversial effects on adipose tissue inflammation. The objective of the study is to examine the direct effect of metformin on adipocyte inflammatory responses and elucidate the underlying mechanisms. Adipocytes were differentiated from 3T3-L1 ce...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1530/JME-17-0066

    authors： Qi T,Chen Y,Li H,Pei Y,Woo SL,Guo X,Zhao J,Qian X,Awika J,Huo Y,Wu C

    更新日期：2017-07-01 00:00:00

  • Changes in progesterone and oestrogen receptor mRNA and protein and oxytocin receptors in endometrium of ewes after intrauterine injection of ovine trophoblast interferon.

    abstract：:This study determined whether twice-daily intrauterine injections of ovine conceptus secretory proteins (oCSP) containing type-I trophoblast interferon (25 micrograms/uterine horn) from day 11 to day 15 post-oestrus (oestrus = day 0) could alter the binding capacities of endometrial receptors for oxytocin, progesteron...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0100185

    authors： Mirando MA,Harney JP,Zhou Y,Ogle TF,Ott TL,Moffatt RJ,Bazer FW

    更新日期：1993-04-01 00:00:00

  • Agonist-bound nuclear receptors: not just targets of coactivators.

    abstract：:Members of the nuclear receptor superfamily of ligand-regulated transcription factors are targets of a wide range of lipophilic signaling molecules as well as several drugs and xenobiotics that modulate many aspects of physiology and metabolism. Agonist binding to receptors is associated with recruitment of coactivato...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1677/jme.0.0310001

    authors： Fernandes I,White JH

    更新日期：2003-08-01 00:00:00

  • Adiponectin limits differentiation and trophoblast invasion in human endometrial cells.

    abstract：:Successful human embryo implantation requires a proper differentiation of endometrial stromal cells (ESCs) into decidual cells, during a process called decidualization. ESCs express specific molecules, such as prolactin, insulin-like growth factor-binding protein-1 (IGFBP-1) and connexin-43. Decidual cells are also in...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1530/JME-17-0046

    authors： Duval F,Dos Santos E,Moindjie H,Serazin V,Swierkowski-Blanchard N,Vialard F,Dieudonné MN

    更新日期：2017-10-01 00:00:00

  • Neuroprotective and anti-ageing role of leptin.

    abstract：:Leptin (Lep), an adipose-derived hormone, exerts very important functions in the body mainly on energy storage and availability. The physiological effects of Lep controlling the body weight and suppressing appetite are mediated by the long form of Lep receptor in the hypothalamus. Lep receptor activates several downst...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1530/JME-12-0151

    authors： Folch J,Pedrós I,Patraca I,Sureda F,Junyent F,Beas-Zarate C,Verdaguer E,Pallàs M,Auladell C,Camins A

    更新日期：2012-10-10 00:00:00

  • Urocortin increased endothelial ICAM1 by cPLA2-dependent NF-κB and PKA pathways in HUVECs.

    abstract：:Urocortin (Ucn1), a member of the corticotrophin-releasing hormone (CRH) family, has been reported to participate in inflammation. The increased expression of intercellular adhesion molecule 1 (ICAM1) plays important roles in inflammation and immune responses. Our previous results demonstrated that Ucn1 significantly ...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1530/JME-13-0182

    authors： Wan R,Liu Y,Li L,Zhu C,Jin L,Li S

    更新日期：2013-12-20 00:00:00

  • Reciprocal influences between the signalling pathways regulating proliferation and steroidogenesis in adrenal glomerulosa cells.

    abstract：:The main regulators of aldosterone secretion in adrenal gland zona glomerulosa (ZG) cells are the hormones angiotensin II (Ang II) and adrenocorticotrophin (ACTH) and small increases in the extracellular potassium (K(+)) concentration. The action of these agonists is mediated by different signalling systems - ACTH is ...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1677/jme.0.0320893

    authors： Foster RH

    更新日期：2004-06-01 00:00:00

  • A novel oestrogen-regulated gene in human breast cancer cells identified by differential display.

    abstract：:Screening by differential display of oestrogen-sensitive MCF7 human breast cancer cells grown in the short-term (6 days) and long-term (70 weeks) absence of oestrogen has led to the identification of a new oestrogen-regulated mRNA. The cDNA isolated by differential display has 100% homology from nucleotides 615 to 859...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0200375

    authors： Stephen R,Corcoran D,Darbre PD

    更新日期：1998-06-01 00:00:00

  • LH receptor RNA and protein levels after hormonal treatment of porcine granulosa cells in primary culture.

    abstract：:Granulosa cells were prepared from small follicles (less than 3 mm) from the ovaries of 5-month-old gilts. They were cultured in plastic dishes coated with a synthetic adhesion peptide in a chemically defined medium supplemented with 2% serum substitute. After 3 days of culture, the cells reached confluence and expres...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0080119

    authors： Goxe B,Salesse R,Remy JJ,Genty N,Garnier J

    更新日期：1992-04-01 00:00:00

  • Uncoupling the mechanisms that facilitate cell survival in hormone-deprived bovine mammary explants.

    abstract：:Mammary explants can be hormonally stimulated to mimic the biochemical changes that occur during lactogenesis. Previous studies using mammary explants concluded that the addition of exogenous macromolecules were required for mammary epithelial cells to remain viable in culture. The present study examines the survival ...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/JME-08-0035

    authors： Brennan AJ,Sharp JA,Lefèvre CM,Nicholas KR

    更新日期：2008-09-01 00:00:00

  • Early regulation of c-myc mRNA by 1,25-dihydroxyvitamin D3 in human myelomonocytic U937 cells.

    abstract：:The effects of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3; 10 nmol/l) on the human monomyelocytic cell line U937 were investigated. Addition of 1,25-(OH)2D3 led to a decrease in cell proliferation which fell at 72 h to 67.8 +/- 4.3% (mean +/- S.E.M.) of control values. The presence of CD14, a surface marker found on matur...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0030043

    authors： Karmali R,Bhalla AK,Farrow SM,Williams MM,Lal S,Lydyard PM,O'Riordan JL

    更新日期：1989-07-01 00:00:00

  • IGF-I inhibits apoptosis through the activation of the phosphatidylinositol 3-kinase/Akt pathway in pituitary cells.

    abstract：:In previous studies we demonstrated that IGF-I induces proliferation of pituitary lactotrophs. In addition to its mitotrophic actions, IGF-I is known to prevent apoptosis induced by diverse stimuli in several cell types. In this study, we investigated the action of IGF-I on pituitary cell survival and the intracellula...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1677/jme.0.0330155

    authors： Fernández M,Sánchez-Franco F,Palacios N,Sánchez I,Fernández C,Cacicedo L

    更新日期：2004-08-01 00:00:00

  • 90 YEARS OF PROGESTERONE: New insights into progesterone receptor signaling in the endometrium required for embryo implantation.

    abstract：:Progesterone's ability to maintain pregnancy in eutherian mammals highlighted this steroid as the 'hormone of pregnancy'. It was the unique 'pro-gestational' bioactivity of progesterone that enabled eventual purification of this ovarian steroid to crystalline form by Willard Myron Allen in the early 1930s. While a fun...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：10.1530/JME-19-0212

    authors： DeMayo FJ,Lydon JP

    更新日期：2020-07-01 00:00:00

  • Dietary restriction of mice on a high-fat diet induces substrate efficiency and improves metabolic health.

    abstract：:High energy intake and, specifically, high dietary fat intake challenge the mammalian metabolism and correlate with many metabolic disorders such as obesity and diabetes. However, dietary restriction (DR) is known to prevent the development of metabolic disorders. The current western diets are highly enriched in fat, ...

    journal_title：Journal of molecular endocrinology

    pub_type： 杂志文章

    doi：

    authors： Duivenvoorde LP,van Schothorst EM,Bunschoten A,Keijer J

    更新日期：2011-08-01 00:00:00