Detection of retinoic acid receptor mRNA in rat tissues by reverse transcriptase-polymerase chain reaction.

abstract：:Obesity is likely to be a multifactorial disease with an important genetic component. Animal models of genetic and experimentally induced obesity suggest that glucocorticoid receptor (GR) activity plays a role in the aetiology and maintenance of the obese state. Glucocorticoid activity appears to be essential for the ...

journal_title：Journal of molecular endocrinology

authors： Weaver JU,Hitman GA,Kopelman PG

更新日期：1992-12-01 00:00:00

abstract：:Cytochrome P450 21-hydroxylase (P450c21) is a key enzyme for corticosteroidogenesis. To understand the regulatory mechanisms of cortisol production in fish, we have cloned a cDNA encoding P450c21, for the first time in non-mammalian vertebrates, from the head kidney of the eel (Anguilla japonica). The overall similari...

journal_title：Journal of molecular endocrinology

authors： Li YY,Inoue K,Takei Y

更新日期：2003-10-01 00:00:00

abstract：:Orexin-A and -B are known to stimulate food intake in mammals. However, the critical roles of orexins in birds are not fully understood, since orexins have no stimulatory effect on food intake in the chicken. To understand the physiological role(s) of orexins in birds, we have cloned chicken orexin receptor (cOXR) cDN...

journal_title：Journal of molecular endocrinology

authors： Ohkubo T,Tsukada A,Shamoto K

更新日期：2003-12-01 00:00:00

abstract：:We investigated the mechanisms by which corticosteroids regulate the expression of the mineralocorticoid receptor (MR) in neurones. Aldosterone and dexamethasone produced a dose-dependent increase of MR and mRNA levels in cultured primary hippocampal neurones. Transient transfection of neuroblastoma cells showed that ...

journal_title：Journal of molecular endocrinology

authors： Castrén M,Trapp T,Berninger B,Castrén E,Holsboer F

更新日期：1995-06-01 00:00:00

abstract：:It has recently been shown that 20 kDa human growth hormone (hGH) forms the 1:2 hGH:hGH receptor (hGHR) complex and expresses full agonistic activity, although it hardly forms the 1:1 GH:GHR complex as compared with 22 kDa hGH. To clarify this mechanism, we analyzed the mode of receptor dimerization of 20 kDa hGH usin...

journal_title：Journal of molecular endocrinology

authors： Uchida H,Banba S,Wada M,Matsumoto K,Ikeda M,Naito N,Tanaka E,Honjo M

更新日期：1999-12-01 00:00:00

abstract：:Both 17beta-estradiol and prolactin play important roles in the mammary gland, raising the possibility of functional cross-talk between the two signaling pathways. Here, we demonstrate that estrogen receptor-alpha (ERalpha) and -beta (ERbeta) are both able to potentiate transcription from a Stat5-responsive promoter w...

journal_title：Journal of molecular endocrinology

authors： Björnström L,Kilic E,Norman M,Parker MG,Sjöberg M

更新日期：2001-08-01 00:00:00

abstract：:The constitutive expression of 25-hydroxyvitamin D3-24-hydroxylase (25-(OH)D3-24-hydroxylase) activity has been studied in an adherent variant (Ad-HL60) of the human promyelomonocytic leukaemia cell line HL60. The Ad-HL60 cells have a more differentiated phenotype than the non-adherent cells from which they were deriv...

journal_title：Journal of molecular endocrinology

authors： Hayes ME,Bayley D,Mawer EB

更新日期：1991-12-01 00:00:00

abstract：:Prolactin (PRL)-releasing peptide (PrRP) is a strong candidate stimulator of pituitary PRL transcription and secretion in teleosts. However, the role in control of extrapituitary PRL expression is unclear even in mammals. To study the possible presence of PrRP-PRL axes not only in the brain-pituitary but also in perip...

journal_title：Journal of molecular endocrinology

authors： Sakamoto T,Amano M,Hyodo S,Moriyama S,Takahashi A,Kawauchi H,Ando M

更新日期：2005-06-01 00:00:00

abstract：:During the process of insulitis in the pathogenesis of type I (insulin-dependent) diabetes mellitus, proinflammatory cytokines induce expression of the death receptor Fas on the surface of pancreatic beta-cells and thereby contribute to the enhanced susceptibility of beta-cells for apoptosis. The aim of this study was...

journal_title：Journal of molecular endocrinology

authors： Augstein P,Wachlin G,Berg S,Bahr J,Salzsieder C,Hehmke B,Heinke P,Salzsieder E

更新日期：2003-04-01 00:00:00

abstract：:Insulin-like growth factor-I (IGF-I) analogues were produced with the aim of identifying IGF-I residues that contribute to the specificity of binding to the type 1 IGF receptor as opposed to the insulin receptor. Receptor binding properties of a series of A- and B-domain analogues were compared using rat L6 myoblasts,...

journal_title：Journal of molecular endocrinology

authors： Shooter GK,Magee B,Soos MA,Francis GL,Siddle K,Wallace JC

更新日期：1996-12-01 00:00:00

abstract：:The mannose 6-phosphate/IGF 2 receptor (IGF2R) is comprised of 15 extra-cellular domains that bind IGF2 and mannose 6-phosphate ligands. IGF2R transports ligands from the Golgi to the pre-lysosomal compartment and thereafter to and from the cell surface. IGF2R regulates growth, placental development, tumour suppressio...

journal_title：Journal of molecular endocrinology

authors： Rezgui D,Williams C,Savage SA,Prince SN,Zaccheo OJ,Jones EY,Crump MP,Hassan AB

更新日期：2009-04-01 00:00:00

abstract：:The parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor regulates extracellular calcium concentrations and is therefore important for mineral homeostasis. ROS 17/2.8 cells, a rat osteoblast-like osteosarcoma cell line, express the PTH/PTHrP receptor and provide a good model for examining the transcriptional...

journal_title：Journal of molecular endocrinology

authors： Williams LJ,Abou-Samra AB

更新日期：2000-12-01 00:00:00

abstract：:Human 3beta-hydroxysteroid dehydrogenase/steroid Delta(5)-Delta(4)-isomerase (3beta-HSD/isomerase) is a bifunctional, single enzyme protein that is membrane-bound in the endoplasmic reticulum (microsomes) and mitochondria of cells in the placenta (type I) and in the adrenals and gonads (type II). Two membrane-binding ...

journal_title：Journal of molecular endocrinology

authors： Thomas JL,Evans BW,Blanco G,Mason JI,Strickler RC

更新日期：1999-10-01 00:00:00

abstract：:Translocation of protein kinase C (PKC) from the cytosol to the plasma membranes is believed to reflect activation of the enzyme. We have studied translocation of PKC in lactotroph-enriched anterior pituitary cell cultures by measuring the incorporation of gamma-32P from [gamma-32P]ATP into a synthetic peptide substra...

journal_title：Journal of molecular endocrinology

authors： Mau SE,Saermark T,Vilhardt H

更新日期：1994-06-01 00:00:00

abstract：:CRH and its structurally related peptide urocortin (Ucn) are released under stress. Ucn is a potent agonist for CRH-receptor 2 (CRH-R2), which is strongly expressed in rodent heart. Stress induces Ucn mRNA expression in the heart, where it may be cardioprotective. However, increasing evidence indicates that Ucn may al...

journal_title：Journal of molecular endocrinology

authors： Huang M,Kempuraj D,Papadopoulou N,Kourelis T,Donelan J,Manola A,Theoharides TC

更新日期：2009-05-01 00:00:00

abstract：:We used the Crm1 inhibitor leptomycin B (LMB) to examine a possible involvement of nuclear export in estrogen receptor alpha (ER) level and function in MCF-7 breast carcinoma cells. As revealed by immunofluorescence microscopy and western blotting with anti-ER antibodies, LMB produced an accumulation of ER in cell nuc...

journal_title：Journal of molecular endocrinology

authors： Nonclercq D,Journé F,Laïos I,Chaboteaux C,Toillon RA,Leclercq G,Laurent G

更新日期：2007-08-01 00:00:00

abstract：:In the common brushtail possum (Trichosurus vulpecula) lactation lasts for 200 days and consists of two distinct phases. Milk composition changes dramatically between phase 2 and 3, which correspond to early and late lactation respectively (phase 1 corresponds to pregnancy). RNA expression patterns have been establish...

journal_title：Journal of molecular endocrinology

authors： Demmer J,Ross IK,Ginger MR,Piotte CK,Grigor MR

更新日期：1998-02-01 00:00:00

abstract：:Progesterone's ability to maintain pregnancy in eutherian mammals highlighted this steroid as the 'hormone of pregnancy'. It was the unique 'pro-gestational' bioactivity of progesterone that enabled eventual purification of this ovarian steroid to crystalline form by Willard Myron Allen in the early 1930s. While a fun...

journal_title：Journal of molecular endocrinology

authors： DeMayo FJ,Lydon JP

更新日期：2020-07-01 00:00:00

abstract：:The diagnosis of conventional and oncocytic poorly differentiated (oPD) thyroid carcinomas is difficult. The aim of this study is to characterise their largely unknown miRNA expression profile and to compare it with well-differentiated thyroid tumours, as well as to identify miRNAs which could potentially serve as dia...

journal_title：Journal of molecular endocrinology

authors： Dettmer MS,Perren A,Moch H,Komminoth P,Nikiforov YE,Nikiforova MN

更新日期：2014-03-06 00:00:00

abstract：:The development of an efficient expression system for insulin-like growth factor-I (IGF-I) in Escherichia coli as a fusion protein is described. The fusion protein consists of an N-terminal extension made up of the first 46 amino acids of methionyl porcine GH ([Met1]-pGH) followed by the dipeptide Val-Asn. The latter ...

journal_title：Journal of molecular endocrinology

authors： King R,Wells JR,Krieg P,Snoswell M,Brazier J,Bagley CJ,Wallace JC,Ballard FJ,Ross M,Francis GL

更新日期：1992-02-01 00:00:00

abstract：:We have studied the action of peripheral blood lymphocytes (PBLs) and intrathyroidal lymphocytes (ITLs) on the biochemical and hormonal metabolism of autologous thyrocytes cultured in follicles in a collagen gel. The production of tumour necrosis factor alpha (TNF-alpha) in culture was also measured. Thyroid tissues a...

journal_title：Journal of molecular endocrinology

authors： Massart C,Gibassier J,Raoul ML,Le Gall F,Lancien G,Genetet N,Denais A,Darcel F,Lucas C

更新日期：1997-04-01 00:00:00

abstract：:Intracellular glucocorticoid (GC) receptor (GR) function determines tissue sensitivity to GCs and strongly affects the development of type 2 diabetes and obesity. 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) mediates intracellular steroid exposure to mouse liver GR by prereceptor reactivation of GCs and is...

journal_title：Journal of molecular endocrinology

authors： Liu Y,Nakagawa Y,Wang Y,Liu L,Du H,Wang W,Ren X,Lutfy K,Friedman TC

更新日期：2008-08-01 00:00:00

abstract：:Atrial natriuretic peptide (ANP) as well as its receptors is found in mammalian ovary and follicular cells and its function in oocyte meiotic maturation has also been reported in Xenopus, hamster and rat. But the results are controversial and the physiological mechanism of ANP on oocyte maturation is not clear, especi...

journal_title：Journal of molecular endocrinology

authors： Zhang M,Tao Y,Zhou B,Xie H,Wang F,Lei L,Huo L,Sun Q,Xia G

更新日期：2005-04-01 00:00:00

abstract：:The nociceptin receptor (NOP) and its endogenous ligand, nociceptin/orphanin FQ (OFQ), are involved in a wide range of biological functions, such as pain, anxiety, learning, and memory. The zebrafish has been proposed as a candidate to study the in vivo effects of several drugs of abuse and to discover new pharmacolog...

journal_title：Journal of molecular endocrinology

authors： Rivas-Boyero AA,Herrero-Turrión MJ,Gonzalez-Nunez V,Sánchez-Simón FM,Barreto-Valer K,Rodríguez RE

更新日期：2011-03-03 00:00:00

abstract：:Twenty-five years have elapsed since the calcium-sensing receptor (CaSR) was first identified in bovine parathyroid and the receptor is now recognized as a fundamental contributor to extracellular Ca2+ (Ca2+ e) homeostasis, regulating parathyroid hormone release and urinary calcium excretion. The CaSR is a class C G-p...

journal_title：Journal of molecular endocrinology

authors： Gorvin CM

更新日期：2019-08-01 00:00:00

abstract：:Inhibition of aromatase, a key enzyme in the biosynthesis of oestradiol-17 beta, by the addition of 1,4,6-androstatrien-3,17-dione resulted in a significant increase in the levels of immunoreactive human chorionic gonadotrophin (hCG) in the medium and tissue. This increase was partially reversed by the simultaneous ad...

journal_title：Journal of molecular endocrinology

authors： Sharma SC,Purohit P,Rao AJ

更新日期：1993-08-01 00:00:00

abstract：:Hypogonadal (hpg) mutant mice, with a congenital deficiency of hypothalamic gonadotrophin-releasing hormone (GnRH), and testicular feminized (tfm) mice, which lack a functional androgen receptor, were used to study the effects of the potent GnRH agonist 'Zoladex' (ICI 118630; D-Ser (Bu(t))6, Azgly10-GnRH) on pituitary...

journal_title：Journal of molecular endocrinology

authors： Scott IS,Bennett MK,Porter-Goff AE,Harrison CJ,Cox BS,Grocock CA,O'Shaughnessy PJ,Clayton RN,Craven R,Furr BJ

更新日期：1992-06-01 00:00:00

abstract：:Reproductive hormone imbalance in infertile women is correlated to high levels of phthalates and alkylphenols, which are among endocrine-disrupting chemicals (EDCs). Previous studies have shown that they interfere with gene expression by deregulating levels of microRNAs (miRs), small non-coding RNAs targeting mRNAs en...

journal_title：Journal of molecular endocrinology

authors： Patiño-García D,Cruz-Fernandes L,Buñay J,Orellana R,Moreno RD

更新日期：2020-11-01 00:00:00

abstract：:Breast cancer is a heterogeneous disease, and the complexity of breast carcinogenesis is associated with epigenetic modification. There are several major classes of epigenetic enzymes that regulate chromatin activity. This review will focus on the nine mammalian protein arginine methyltransferases (PRMTs) and the dysr...

journal_title：Journal of molecular endocrinology

authors： Wang SM,Dowhan DH,Muscat GEO

更新日期：2019-04-01 00:00:00

abstract：:Genetic studies have reported association of a polyglycine-encoding GGN repeat in exon 1 of the androgen receptor (AR) gene with common human traits. The polyglycine tract is located in the transactivating domain of the AR protein, suggesting an effect of repeat length on receptor function. Here, we compare the functi...

journal_title：Journal of molecular endocrinology

authors： Brockschmidt FF,Nöthen MM,Hillmer AM

更新日期：2007-07-01 00:00:00