Cloning and characterization of cDNAs encoding steroidogenic acute regulatory protein from freshwater stingrays (Potamotrygon spp.).


:The steroidogenic acute regulatory protein (StAR) is critical to the regulated synthesis of steroids in vertebrates. We have isolated cDNA sequences encoding StAR in the freshwater stingrays Potamotrygon hystrix and P. motoro. A single P. hystrix StAR transcript (3376 bp) and two overlapping P. motoro StAR transcripts (1272 and 3365 bp) were isolated. The P. hystrix and P. motoro StAR transcripts contain open reading frames encoding proteins of 284 amino acids that are 99% identical to each other and 56-64% identical to other StAR proteins. Pregnenolone synthesis by green monkey kidney (COS-1) cells transfected with an expression construct encoding a human cholesterol side chain cleavage/adrenodoxin reductase/adrenodoxin fusion protein was increased 16-fold by coexpression with a pCMV5/P. motoro StAR expression construct. Northern blot analysis revealed a single 4000 bp StAR transcript in the P. motoro interrenal gland, but RT-PCR indicates StAR mRNA is also expressed in the brain, gonads, atria, ventricle, gill (female only) and muscle (female only). Expression in extragonadal and extraadrenocortical tissues is an indication that StAR may be critical to processes other than steroidogenesis. The longest P. motoro StAR transcript contains a sequence with great similarity to short interspersed repetitive elements found in other elasmobranchs. This study is the first to isolate and characterize elasmobranch StAR cDNA sequences and to demonstrate the activity of a nonmammalian StAR protein in a heterologous expression system.


J Mol Endocrinol


Nunez BS,Piermarini PM,Evans AN,Applebaum SL




Has Abstract


2005-12-01 00:00:00














  • Acute anorexigenic action of leptin in rainbow trout is mediated by the hypothalamic Pi3k pathway.

    abstract::Leptin (Lep) is an anorexigenic hormone and regulates appetite-related neuropeptides in mammals. A number of neuropeptides have also been linked to appetite regulation in teleost fish, but Lep signaling activation and effects on appetite-regulating neurons are poorly elucidated in early vertebrates. This study uses ce...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Gong N,Jönsson E,Björnsson BT

    更新日期:2016-04-01 00:00:00

  • Semi-quantitative analysis of interleukin-1 alpha, interleukin-6 and interleukin-8 mRNA expression by human thyrocytes.

    abstract::It has been suggested that the thyroid itself may contribute to the inflammatory process observed in autoimmune thyroiditis by releasing the cytokines interleukin-1 alpha (IL-1 alpha), interleukin-6 (IL-6) and interleukin-8 (IL-8), but studies of cytokine gene expression in thyrocytes have been limited and conflicting...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Watson PF,Pickerill AP,Davies R,Weetman AP

    更新日期:1995-08-01 00:00:00

  • cDNA cloning of chicken orexin receptor and tissue distribution: sexually dimorphic expression in chicken gonads.

    abstract::Orexin-A and -B are known to stimulate food intake in mammals. However, the critical roles of orexins in birds are not fully understood, since orexins have no stimulatory effect on food intake in the chicken. To understand the physiological role(s) of orexins in birds, we have cloned chicken orexin receptor (cOXR) cDN...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Ohkubo T,Tsukada A,Shamoto K

    更新日期:2003-12-01 00:00:00

  • Effect of nuclear export inhibition on estrogen receptor regulation in breast cancer cells.

    abstract::We used the Crm1 inhibitor leptomycin B (LMB) to examine a possible involvement of nuclear export in estrogen receptor alpha (ER) level and function in MCF-7 breast carcinoma cells. As revealed by immunofluorescence microscopy and western blotting with anti-ER antibodies, LMB produced an accumulation of ER in cell nuc...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Nonclercq D,Journé F,Laïos I,Chaboteaux C,Toillon RA,Leclercq G,Laurent G

    更新日期:2007-08-01 00:00:00

  • Localization of inhibin/activin subunit mRNAs during the luteal phase in the primate ovary.

    abstract::Localization of inhibin/activin subunit mRNAs within the macaque ovary from the immediate pre-ovulatory period of the menstrual cycle, when serum immunoreactive inhibin begins to rise, to day 9 of the luteal phase, when serum inhibin concentrations are maximal, was investigated using in-situ hybridization. Ovaries wer...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Fraser HM,Lunn SF,Cowen GM,Saunders PT

    更新日期:1993-06-01 00:00:00

  • Neuroendocrine integration of nutritional signals on reproduction.

    abstract::Reproductive function in mammals is energetically costly and therefore tightly regulated by nutritional status. To enable this integration of metabolic and reproductive function, information regarding peripheral nutritional status must be relayed centrally to the gonadotropin-releasing hormone (GNRH) neurons that driv...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章,评审


    authors: Evans MC,Anderson GM

    更新日期:2017-02-01 00:00:00

  • Glucocorticoid regulation of amino acid transport in primary human trophoblast cells.

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    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Vaughan OR,Powell TL,Jansson T

    更新日期:2019-11-01 00:00:00

  • An association between a Bc1I restriction fragment length polymorphism of the glucocorticoid receptor locus and hyperinsulinaemia in obese women.

    abstract::Obesity is likely to be a multifactorial disease with an important genetic component. Animal models of genetic and experimentally induced obesity suggest that glucocorticoid receptor (GR) activity plays a role in the aetiology and maintenance of the obese state. Glucocorticoid activity appears to be essential for the ...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Weaver JU,Hitman GA,Kopelman PG

    更新日期:1992-12-01 00:00:00

  • SULFATION PATHWAYS: Contribution of intracrine oestrogens to the aetiology of endometriosis.

    abstract::Endometriosis is an incurable hormone-dependent inflammatory disease that causes chronic pelvic pain and infertility characterized by implantation and growth of endometrial tissue outside the uterine cavity. Symptoms have a major impact on the quality of life of patients resulting in socioeconomic, physical and psycho...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章,评审


    authors: Piccinato CA,Malvezzi H,Gibson DA,Saunders PTK

    更新日期:2018-08-01 00:00:00

  • Transcriptional upregulation of hepatic GH receptor and GH-binding protein expression during pregnancy in the mouse.

    abstract::In the mouse, GH-binding protein (GHBP) and GH receptor (GHR) are encoded by a single gene via alternative splicing. We previously demonstrated that the steady-state levels of the GHR and GHBP mRNAs are significantly elevated in mouse liver during pregnancy. Hepatic GHR and GHBP mRNAs are associated primarily with one...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Ilkbahar YN,Southard JN,Talamantes F

    更新日期:1999-08-01 00:00:00

  • miR-135a-5p inhibits 3T3-L1 adipogenesis through activation of canonical Wnt/β-catenin signaling.

    abstract::MicroRNAs are endogenous, conserved, and non-coding small RNAs that function as post-transcriptional regulators of fat development and adipogenesis. Adipogenic marker genes, such as CCAAT/enhancer binding protein α (Cebpa), peroxisome proliferator-activated receptor γ (Pparg), adipocyte fatty acid binding protein (Ap2...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Chen C,Peng Y,Peng Y,Peng J,Jiang S

    更新日期:2014-06-01 00:00:00

  • Organization of the Indian hedgehog--parathyroid hormone-related protein system in the postnatal growth plate.

    abstract::In embryonic growth cartilage, Indian hedgehog (Ihh) and parathyroid hormone-related protein (PTHrP) participate in a negative feedback loop that regulates chondrocyte differentiation. Postnatally, this region undergoes major structural and functional changes. To explore the organization of the Ihh–PTHrP system in pos...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Chau M,Forcinito P,Andrade AC,Hegde A,Ahn S,Lui JC,Baron J,Nilsson O

    更新日期:2011-08-01 00:00:00

  • Tumour necrosis factor superfamily member 11 gene promoter polymorphisms modulate promoter activity and influence bone mineral density in postmenopausal women with osteoporosis.

    abstract::Tumour necrosis factor superfamily member 11 (TNFSF11) gene, that codes for receptor activator of nuclear factor-kappaB ligand, is one of the candidate genes for the genetic susceptibility to osteoporosis. As variations in the TNFSF11 gene promoter could alter its expression, the aim of the study was to evaluate the f...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Mencej S,Albagha OM,Prezelj J,Kocjan T,Marc J

    更新日期:2008-06-01 00:00:00

  • IGF-independent effects of IGFBP-2 on the human breast cancer cell line Hs578T.

    abstract::There is evidence that insulin-like growth factor-binding protein (IGFBP-2), a modulator of the actions of IGFs, also has IGF-independent effects in human tumor cell lines. These involve specific binding of IGFBP-2 to alpha5beta1-integrin, followed by alterations in the phosphorylation status of downstream signaling m...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Frommer KW,Reichenmiller K,Schutt BS,Hoeflich A,Ranke MB,Dodt G,Elmlinger MW

    更新日期:2006-08-01 00:00:00

  • The PROGINS polymorphism of the human progesterone receptor diminishes the response to progesterone.

    abstract::The human progesterone receptor (PR) is a ligand-dependent transcription factor and two isoforms, (PRA and PRB), can be distinguished. PROGINS, a PR polymorphic variant, affects PRA and PRB and acts as a risk-modulating factor in several gynaecological disorders. Little is known about the functional consequences of th...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Romano A,Delvoux B,Fischer DC,Groothuis P

    更新日期:2007-02-01 00:00:00

  • Epigenetic arginine methylation in breast cancer: emerging therapeutic strategies.

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    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章,评审


    authors: Wang SM,Dowhan DH,Muscat GEO

    更新日期:2019-04-01 00:00:00

  • 17beta-hydroxysteroid dehydrogenase (HSD)/17-ketosteroid reductase (KSR) family; nomenclature and main characteristics of the 17HSD/KSR enzymes.

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    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章,评审


    authors: Peltoketo H,Luu-The V,Simard J,Adamski J

    更新日期:1999-08-01 00:00:00

  • The ARP-1 orphan receptor represses steroid-mediated stimulation of human placental lactogen gene expression.

    abstract::Studies were performed to determine whether ARP-1, which is an orphan receptor of the steroid receptor superfamily, inhibits basal activity of the human placental lactogen (hPL) promoter and the increase in hPL promoter activity in response to the receptors for thyroid hormone (TR) and retinoic acid (RAR). Co-transfec...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Stephanou A,Shah M,Richardson B,Handwerger S

    更新日期:1996-06-01 00:00:00

  • Recent insight into the correlation of SREBP-mediated lipid metabolism and innate immune response

    abstract::Fatty acids are essential nutrients that contribute to several intracellular functions. Fatty acid synthesis and oxidation are known to be regulated by sterol regulatory element-binding proteins (SREBPs), which play a pivotal role in the regulation of cellular triglyceride synthesis and cholesterol biogenesis. Recent ...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章,评审


    authors: Park HY,Kang HS,Im SS

    更新日期:2018-10-01 00:00:00

  • Aromatase expression in Xenopus oocytes: a three cell-type model for the ovarian estradiol synthesis.

    abstract::In contrast to the classical model describing the synthesis of androgens and estrogens as restricted to somatic cells, a previous study demonstrated that Xenopus laevis oocytes participate in androgen synthesis. The objective of our study was to determine whether Xenopus oocytes are also involved in estrogen synthesis...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Gohin M,Bodinier P,Fostier A,Bobe J,Chesnel F

    更新日期:2011-09-07 00:00:00

  • Thyroid hormone-mediated negative transcriptional regulation of Necdin expression.

    abstract::Unliganded thyroid hormone receptors (apoTRs) repress transcription of hormone-activated genes by recruiting corepressors to the promoters. In contrast, on promoters containing so-called negative thyroid hormone response elements (nTREs), apoTRs activate transcription. A number of different molecular mechanisms have b...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Nygård M,Becker N,Demeneix B,Pettersson K,Bondesson M

    更新日期:2006-06-01 00:00:00

  • Uncoupling the mechanisms that facilitate cell survival in hormone-deprived bovine mammary explants.

    abstract::Mammary explants can be hormonally stimulated to mimic the biochemical changes that occur during lactogenesis. Previous studies using mammary explants concluded that the addition of exogenous macromolecules were required for mammary epithelial cells to remain viable in culture. The present study examines the survival ...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Brennan AJ,Sharp JA,Lefèvre CM,Nicholas KR

    更新日期:2008-09-01 00:00:00

  • Cloning of rabbit prolactin cDNA and prolactin gene expression in the rabbit mammary gland.

    abstract::cDNA clones coding for rabbit prolactin were isolated from a pituitary library using a rat prolactin RNA probe. One cDNA contained 873 bases including the entire coding sequence of rabbit prolactin, its signal peptide and the 5' and 3' untranslated regions of 44 and 145 nucleotides respectively. The deduced amino acid...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Gabou L,Boisnard M,Gourdou I,Jammes H,Dulor JP,Djiane J

    更新日期:1996-02-01 00:00:00

  • The interaction of CGRP and adrenomedullin with a receptor expressed in the rat pulmonary vascular endothelium.

    abstract::An abundant, seven trans-membrane domain receptor related to the calcitonin receptor has been studied by a number of groups without identification of its ligand. A recent report claimed that the receptor was a type 1 CGRP receptor (Aiyar et al J. Biol. Chem. 271 11325-11329 (1996)). We have studied the equivalent rat ...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Han ZQ,Coppock HA,Smith DM,Van Noorden S,Makgoba MW,Nicholl CG,Legon S

    更新日期:1997-06-01 00:00:00

  • Daily exposure to phthalates and alkylphenols alters miR biogenesis and expression in mice ovaries.

    abstract::Reproductive hormone imbalance in infertile women is correlated to high levels of phthalates and alkylphenols, which are among endocrine-disrupting chemicals (EDCs). Previous studies have shown that they interfere with gene expression by deregulating levels of microRNAs (miRs), small non-coding RNAs targeting mRNAs en...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Patiño-García D,Cruz-Fernandes L,Buñay J,Orellana R,Moreno RD

    更新日期:2020-11-01 00:00:00

  • Detection of retinoic acid receptor mRNA in rat tissues by reverse transcriptase-polymerase chain reaction.

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    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Wan YJ,Wang L,Wu TC

    更新日期:1992-12-01 00:00:00

  • Cloning and characterization of goldfish activin type IIB receptor.

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    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Ge W,Tanaka M,Yoshikuni M,Eto Y,Nagahama Y

    更新日期:1997-08-01 00:00:00

  • Effects of sex steroids on gonadotropin (FSH and LH) regulation in coho salmon (Oncorhynchus kisutch).

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    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Dickey JT,Swanson P

    更新日期:1998-12-01 00:00:00

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    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Mirando MA,Harney JP,Zhou Y,Ogle TF,Ott TL,Moffatt RJ,Bazer FW

    更新日期:1993-04-01 00:00:00

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    abstract::Tight regulation of fatty acid metabolism in pancreatic beta-cells is important for beta-cell viability and function. Chronic exposure to elevated concentrations of fatty acid is associated with beta-cell lipotoxicity. Glucose is known to repress fatty acid oxidation and hence to augment the toxicity of fatty acids. T...

    journal_title:Journal of molecular endocrinology

    pub_type: 杂志文章


    authors: Ravnskjaer K,Boergesen M,Dalgaard LT,Mandrup S

    更新日期:2006-04-01 00:00:00