Abstract：
:Metformin improves obesity-associated metabolic dysregulation, but has controversial effects on adipose tissue inflammation. The objective of the study is to examine the direct effect of metformin on adipocyte inflammatory responses and elucidate the underlying mechanisms. Adipocytes were differentiated from 3T3-L1 cells and treated with metformin at various doses and for different time periods. The treated cells were examined for the proinflammatory responses, as well as the phosphorylation states of AMPK and the expression of PFKFB3/iPFK2. In addition, PFKFB3/iPFK2-knockdown adipocytes were treated with metformin and examined for changes in the proinflammatory responses. The following results were obtained from the study. Treatment of adipocytes with metformin decreased the effects of lipopolysaccharide on inducing the phosphorylation states of JNK p46 and on increasing the mRNA levels of IL-1β and TNFα. In addition, treatment with metformin increased the expression of PFKFB3/iPFK2, but failed to significantly alter the phosphorylation states of AMPK. In PFKFB3/iPFK2-knockdown adipocytes, treatment with metformin did not suppress the proinflammatory responses as did it in control adipocytes. In conclusion, metformin has a direct effect on suppressing adipocyte proinflammatory responses in an AMPK-independent manner. Also, metformin increases adipocyte expression of PFKFB3/iPFK2, which is involved in the anti-inflammatory effect of metformin.
journal_name
J Mol Endocrinoljournal_title
Journal of molecular endocrinologyauthors
Qi T,Chen Y,Li H,Pei Y,Woo SL,Guo X,Zhao J,Qian X,Awika J,Huo Y,Wu Cdoi
10.1530/JME-17-0066subject
Has Abstractpub_date
2017-07-01 00:00:00pages
49-59issue
1eissn
0952-5041issn
1479-6813pii
59/1/49journal_volume
59pub_type
杂志文章abstract：:Porcine LH receptor ectodomain was overexpressed in insect cells and lepidopteran larvae using the recombinant baculovirus expression system. A low multiplicity of infection yielded the largest active production, of approximately 10(7) receptors/cell or 3 micrograms active receptor/mg total protein in infected cells. ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0140051
更新日期：1995-02-01 00:00:00
abstract：:Successful human embryo implantation requires a proper differentiation of endometrial stromal cells (ESCs) into decidual cells, during a process called decidualization. ESCs express specific molecules, such as prolactin, insulin-like growth factor-binding protein-1 (IGFBP-1) and connexin-43. Decidual cells are also in...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-17-0046
更新日期：2017-10-01 00:00:00
abstract：:CRH and its structurally related peptide urocortin (Ucn) are released under stress. Ucn is a potent agonist for CRH-receptor 2 (CRH-R2), which is strongly expressed in rodent heart. Stress induces Ucn mRNA expression in the heart, where it may be cardioprotective. However, increasing evidence indicates that Ucn may al...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/JME-08-0120
更新日期：2009-05-01 00:00:00
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journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/JME-08-0149
更新日期：2009-03-01 00:00:00
abstract：:Atrial natriuretic peptide (ANP) as well as its receptors is found in mammalian ovary and follicular cells and its function in oocyte meiotic maturation has also been reported in Xenopus, hamster and rat. But the results are controversial and the physiological mechanism of ANP on oocyte maturation is not clear, especi...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.1.01673
更新日期：2005-04-01 00:00:00
abstract：:During the process of insulitis in the pathogenesis of type I (insulin-dependent) diabetes mellitus, proinflammatory cytokines induce expression of the death receptor Fas on the surface of pancreatic beta-cells and thereby contribute to the enhanced susceptibility of beta-cells for apoptosis. The aim of this study was...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0300163
更新日期：2003-04-01 00:00:00
abstract：:Hypothalamic GHRH controls the release of GH from the pituitary gland and also acts as a growth factor in a variety of cancers. The mitogenetic activity of GHRH is exerted through the binding to the pituitary type receptor (pGHRH-R) and its splice variants, mainly SV1. The intracellular signaling pathways that are act...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/JME-09-0121
更新日期：2010-02-01 00:00:00
abstract：:The classical concept of the renin-angiotensin system (RAS) is that of a blood-borne cascade, whose final and bioactive product, angiotensin II, plays an important endocrine role in the maintenance of blood pressure and electrolyte as well as fluid balance. In addition to this circulating RAS, there are an increasing ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1677/jme.0.0260155
更新日期：2001-06-01 00:00:00
abstract：:Chemotactic cytokines (chemokines) are small secreted proteins that control leukocyte trafficking in immune organs. Chemokines which are induced in the brain during conditions of inflammation play a role in the local immune response. Recently, it has been established in the rodent brain that distinct chemokines and ch...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1677/JME-06-0032
更新日期：2007-03-01 00:00:00
abstract：:Expression of the rat alpha 2u-globulin gene family is regulated in the adult male liver by a number of hormones, including growth hormone, thyroid hormone and several steroids. Upon injection into ovariectomized females, estrogens first induce alpha 2u-globulin expression and then suppress this gene after several day...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0200141
更新日期：1998-02-01 00:00:00
abstract：:Bisphenol A (BPA) is a xenobiotic endocrine-disrupting chemical. In vitro and in vivo studies have indicated that BPA alters endocrine-metabolic pathways in adipose tissue, which increases the risk of metabolic disorders and obesity. BPA can affect adipose tissue and increase fat cell numbers or sizes by regulating th...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-14-0282
更新日期：2015-06-01 00:00:00
abstract：:The human progesterone receptor (PR) is a ligand-dependent transcription factor and two isoforms, (PRA and PRB), can be distinguished. PROGINS, a PR polymorphic variant, affects PRA and PRB and acts as a risk-modulating factor in several gynaecological disorders. Little is known about the functional consequences of th...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.1.02170
更新日期：2007-02-01 00:00:00
abstract：:The parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor regulates extracellular calcium concentrations and is therefore important for mineral homeostasis. ROS 17/2.8 cells, a rat osteoblast-like osteosarcoma cell line, express the PTH/PTHrP receptor and provide a good model for examining the transcriptional...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0250309
更新日期：2000-12-01 00:00:00
abstract：:The GnRH receptor (GnRHR) is expressed in several non-pituitary tissues, notably in gonads. However, mechanisms underlying the gonad-specific expression of Gnrhr are not well understood. Here, Gnrhr expression was analysed in the developing testes and pituitaries of rats and transgenic mice bearing the human placental...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-12-0231
更新日期：2013-04-23 00:00:00
abstract：:The effect of steroid hormone treatment on coho salmon (Oncorhynchus kisutch) was examined. The cDNAs for coho salmon FSH beta and LH beta subunits were cloned and sequenced using reverse transcriptase PCR. Northern blot analysis revealed that a single transcript of 1 kb for each of these subunits was present in the p...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0210291
更新日期：1998-12-01 00:00:00
abstract：:Somatostatin (SRIF) is commonly regarded as an inhibitor of GH release in rodents and humans. However, in pigs, SRIF can stimulate the release of GH at low (picomolar) doses, while inhibiting GHRH-stimulated GH release at high (nanomolar) doses in primary pituitary cell cultures. One possible mechanism by which pig ce...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0320437
更新日期：2004-04-01 00:00:00
abstract：:Protein kinase C (PKC) has been identified in islets of Langerhans and insulin-secreting tumour cells. Diacylglycerols (DAGs, the endogenous PKC activators) are generated in response to insulin secretagogues, although nutrient and non-nutrient secretagogues generate DAGs of different compositions and of different pote...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1677/jme.0.0060121
更新日期：1991-04-01 00:00:00
abstract：:cDNAs encoding the glycoprotein hormone alpha- and gonadotropin (GTH) II beta-subunits of Japanese eel (Anguilla japonica) pituitary were cloned using the polymerase chain reaction. The nucleotide sequence of the glycoprotein hormone alpha-subunit cDNA was 364 base pairs (bp) long, encoding 117 amino acids, and that o...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0160171
更新日期：1996-04-01 00:00:00
abstract：:The crystal structures of the leucine-rich repeat domain (LRD) of the FSH receptor (FSHR) in complex with FSH and the TSH receptor (TSHR) LRD in complex with the thyroid-stimulating autoantibody (M22) provide opportunities to assess the molecular basis of the specificity of glycoprotein hormone-receptor binding. A com...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/JME-08-0040
更新日期：2008-09-01 00:00:00
abstract：:Basal and thyroid hormone-regulated expression of constructs containing varying lengths of 5' flanking sequence of the human glycoprotein hormone alpha subunit gene ligated to the reporter gene encoding chloramphenicol acetyl transferase (CAT) were examined in transfected pituitary GH3 cells. CAT gene expression was q...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0040187
更新日期：1990-04-01 00:00:00
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journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0200375
更新日期：1998-06-01 00:00:00
abstract：:Evidence indicates that central regulation of food intake is well conserved along the vertebrate lineage, at least between teleost fish and mammals. However, several differences arise in the comparison between both groups. In this review, we describe similarities and differences between teleost fish and mammals on an ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1530/JME-17-0320
更新日期：2018-05-01 00:00:00
abstract：:Studies were performed to determine whether ARP-1, which is an orphan receptor of the steroid receptor superfamily, inhibits basal activity of the human placental lactogen (hPL) promoter and the increase in hPL promoter activity in response to the receptors for thyroid hormone (TR) and retinoic acid (RAR). Co-transfec...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0160221
更新日期：1996-06-01 00:00:00
abstract：:The development of an efficient expression system for insulin-like growth factor-I (IGF-I) in Escherichia coli as a fusion protein is described. The fusion protein consists of an N-terminal extension made up of the first 46 amino acids of methionyl porcine GH ([Met1]-pGH) followed by the dipeptide Val-Asn. The latter ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0080029
更新日期：1992-02-01 00:00:00
abstract：:Reproductive hormone imbalance in infertile women is correlated to high levels of phthalates and alkylphenols, which are among endocrine-disrupting chemicals (EDCs). Previous studies have shown that they interfere with gene expression by deregulating levels of microRNAs (miRs), small non-coding RNAs targeting mRNAs en...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-20-0149
更新日期：2020-11-01 00:00:00
abstract：:Molecular genetics and other contemporary approaches have contributed to a better understanding of prolactin (PRL) actions at the cellular and organismal levels. In this review, several advances in knowledge of PRL actions are highlighted. Special emphasis is paid to areas of progress with consequences for understandi...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1530/JME-13-0220
更新日期：2013-12-19 00:00:00
abstract：:The diagnosis of conventional and oncocytic poorly differentiated (oPD) thyroid carcinomas is difficult. The aim of this study is to characterise their largely unknown miRNA expression profile and to compare it with well-differentiated thyroid tumours, as well as to identify miRNAs which could potentially serve as dia...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-13-0266
更新日期：2014-03-06 00:00:00
abstract：:Natriuretic peptides (NPs) have been reported to have critical roles in follicular development and oocyte maturation in rodents. This study aimed to extend our current understanding of NP-mediated signalling pathways and mechanisms of action in the follicles of a monovulatory species. Ovine granulosa cells (GCs) and t...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-20-0034
更新日期：2020-05-01 00:00:00
abstract：:This study investigates the effectiveness and mechanisms of a serum- and glucocorticoid-inducible kinase 1 (SGK1) inhibitor in counteracting hyperglycemia. In an in vivo experiment, we demonstrated that after an 8-week treatment with an SGK1 inhibitor, the fasting blood glucose and HbA1c level significantly decreased ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-15-0285
更新日期：2016-05-01 00:00:00
abstract：:In vertebrates, sex is determined essentially by two means, genetic factors located on sex chromosomes and epigenetic factors such as temperature experienced by the individual during development. Steroids, especially estrogens, are clearly involved in gonadal differentiation in non-mammalian vertebrates. In this regar...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.1.01588
更新日期：2004-12-01 00:00:00
