Abstract:
:Basal and thyroid hormone-regulated expression of constructs containing varying lengths of 5' flanking sequence of the human glycoprotein hormone alpha subunit gene ligated to the reporter gene encoding chloramphenicol acetyl transferase (CAT) were examined in transfected pituitary GH3 cells. CAT gene expression was quantified by measurement of CAT enzyme activity. In addition, an RNase protection assay was used to confirm changes in CAT mRNA levels and to verify regulation of transcription from the authentic alpha gene start site. The pattern of basal alpha promoter activity indicated the presence of inhibitory DNA elements between -98 and -172 and upstream of -846 relative to the transcriptional start site, as well as a stimulatory element between -346 and -846. Each of the transfected constructs exhibited tri-iodothyronine inhibition of alpha promoter activity, suggesting the presence of an inhibitory thyroid hormone response element within 98 bp of the alpha gene transcriptional start site.
journal_name
J Mol Endocrinoljournal_title
Journal of molecular endocrinologyauthors
Balfour NJ,Franklyn JA,Gurr JA,Sheppard MCdoi
10.1677/jme.0.0040187subject
Has Abstractpub_date
1990-04-01 00:00:00pages
187-90issue
2eissn
0952-5041issn
1479-6813journal_volume
4pub_type
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