Abstract：
:The use of 8-methoxypsoralen (8-MOP) and UV-A irradiation to inactivate contaminating donor leukocytes in platelet concentrates and to prevent primary alloimmunization against donor class I major histocompatibility (MHC) antigens in mice was investigated. CBA/CaH-T6J mice with the H2k haplotype and BALB/cByJ mice with the H2d haplotype were used as donors and recipients, respectively. The mixed leukocyte reaction between these two strains of mice showed that treatment of spleen cells with 500 ng/mL 8-MOP and 5J/cm2 UV-A inhibited 99% of responder and 92% of stimulator function. There was no measurable loss of platelet aggregating activity after the treatment. After two weekly transfusions of platelets without any treatment, 93% of control mice (n = 15) developed anti-H2k antibody. In contrast, only 33% of mice (n = 15) receiving platelets treated with 8-MOP and UV-A became alloimmunized. After six weekly platelet transfusions, all mice became alloimmunized. Nevertheless, the mean titers of anti-H2k antibody in sera of the treated groups were significantly lower than the control groups. One hour posttransfusion recoveries of 51Cr-labeled donor platelets were also higher in mice transfused with the treated platelets. Thus, the pretreatment of platelet concentrates with 8-MOP and UV-A irradiation effectively reduced the alloantigenicity of class I MHC molecules. The implication of this finding in relation to the mechanism by which donor leukocytes allosensitize recipients is discussed.
journal_name
Bloodjournal_title
Bloodauthors
Grana NH,Kao KJsubject
Has Abstractpub_date
1991-06-01 00:00:00pages
2530-7issue
11eissn
0006-4971issn
1528-0020journal_volume
77pub_type
杂志文章相关文献
BLOOD文献大全abstract：:All-trans retinoic acid (ATRA) has previously been shown to inhibit the growth of OPM-2 human myeloma cells. The growth inhibition was postulated to result from a transcriptional downregulation of interleukin-6 receptor alpha (IL-6Ralpha) with IL-6Rbeta (gp130) unaffected. To formally test this hypothesis, an expressi...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:We used a sensitive real-time reverse transcription-polymerase chain reaction assay to quantify cyclin D1 mRNA levels in bone marrow samples collected at diagnosis from 74 newly diagnosed multiple myeloma (MM) patients who were randomized to undergo either single or double autologous peripheral blood stem cell transpl...
journal_title：Blood
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1182/blood-2002-12-3789
更新日期：2003-09-01 00:00:00
abstract：:Although the critical requirement for the transcription factor RUNX1/AML1 at the onset of hematopoietic development is well established, little is known about its transcriptional targets at this pivotal stage of blood development. Using microarrays, we identified the uncharacterized gene AI467606 as a gene whose expre...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2010-06-294124
更新日期：2011-07-21 00:00:00
abstract：:Fourteen patients with cytogenetic relapse of chronic myeloid leukemia (CML) after transplantation with unmanipulated bone marrow were treated with alpha-2a-interferon. There were eight men and six women, median age, 33 years. Twelve patients received marrow from a related allogeneic donor and two received marrow from...
journal_title：Blood
pub_type： 临床试验,杂志文章
doi：
更新日期：1997-10-01 00:00:00
abstract：:Two fractions of human prothrombin can be isolated from single donor plasma by the technique of heparin-agarose chromatography in (sodium) citrate buffer, pH 7.5, as previously reported for pooled plasma. The two fractions, designated H-II1 and H-II2, are found in a ratio of approximately 4:1. Both forms comigrate in ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1981-11-01 00:00:00
abstract：:Posttransplantation lymphoproliferative disease (PTLD) associated with Epstein-Barr virus (EBV) is a life-threatening complication after allogeneic hematopoietic stem cell transplantation. PTLD is efficiently prevented by adoptive transfer of EBV-specific T cells from the donor. To make EBV-specific T cells available ...
journal_title：Blood
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1182/blood-2009-08-236356
更新日期：2010-04-08 00:00:00
abstract：:We studied the cell kill induced by granulocyte-macrophage colony-stimulating factor (GM-CSF ) fused to Diphtheria Toxin (DT-GM-CSF ) in acute myeloid leukemia (AML) samples and in populations of normal primitive hemopoietic progenitor cells. AML samples from three patients were incubated in vitro with 100 ng/mL DT-GM...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:The spread of multiple myeloma (MM) involves (re)circulation into the peripheral blood and (re)entrance or homing of MM cells into new sites of the BM. Hypoxia in solid tumors was shown to promote metastasis through activation of proteins involved in the epithelial-mesenchymal transition (EMT) process. We hypothesized...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2011-09-380410
更新日期：2012-06-14 00:00:00
abstract：:CD8+CD25+ cells, which expressed high levels of Foxp3, glucocorticoid-induced tumor necrosis factor receptor (GITR), CCR8, tumor necrosis factor receptor 2 (TNFR2), and cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) mRNAs, were identified in the fibrous septa and medullary areas of human thymus. Activated CD8+CD...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2003-04-1320
更新日期：2003-12-01 00:00:00
abstract：:In patients with common variable immunodeficiency (CVI), we have previously defined a subgroup of patients (CVIHyper) characterized by decreased numbers of CD4+ lymphocytes in peripheral blood, splenomegaly, and persistent immune activation in vivo, particularly of monocytes/macrophages. To further characterize this h...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1996-01-15 00:00:00
abstract：:A semiquantitative polymerase chain reaction assay was used to monitor the blood levels of Epstein-Barr virus (EBV)-DNA in 9 patients receiving allogeneic bone marrow transplants (BMT). Four of 5 recipients of HLA-mismatched T-cell-depleted grafts showed a 4- to 5-log increase of EBV-DNA within 1 to 3 months after BMT...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：:A 14-year-old boy with hemoglobin SC disease and alpha-thalassemia-2 experienced five episodes of acute splenic sequestration crisis (ASSC), while two of his siblings with identical globin genotypes (SC and -alpha/alpha alpha) had no such experience. To determine if an additional red blood cell (RBC) defect was respon...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:We have previously demonstrated that young adult DBA/2 (DBA) mice have more stem cells than C57BL/6 (B6) mice, as measured in a cobblestone area-forming cell (CAFC) assay using unfractionated marrow. To study the nature of this difference, we have now compared the proliferative fate of single, highly enriched Sca-1(+)...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：2000-08-15 00:00:00
abstract：:The unique t(15;17) of acute promyelocytic leukemia (APL) fuses the PML gene with the retinoic acid receptor alpha (RAR alpha) gene. Although retinoic acid (RA) inhibits cell growth and induces differentiation in human APL cells, resistance to RA develops both in vitro and in patients. We have developed RA-resistant s...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1997-06-15 00:00:00
abstract：:Recent studies suggest that the evolutionary history of a cancer is important in forecasting clinical outlook. To gain insight into the clonal dynamics of multiple myeloma (MM) and its possible influence on patient outcomes, we analyzed whole exome sequencing tumor data for 333 patients from Myeloma XI, a UK phase 3 t...
journal_title：Blood
pub_type： 杂志文章,随机对照试验
doi：10.1182/blood-2016-11-750612
更新日期：2017-10-05 00:00:00
abstract：:Marrow specimens obtained from 23 patients with acute myelocytic leukemia were exposed to cytosine arabinoside and/or daunorubicin in vitro, and the effects of these agents on colony formation in vitro was determined. Thymidine suicide indices were determined as well, which permitted a distinction to be made between k...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1980-09-01 00:00:00
abstract：:p15(INK4b) gene is an inhibitor of cyclin-dependent kinase (CDK) 4 and CDK6 whose expression is induced by transforming growth factor (TGF)beta. Recent reports suggest frequent methylation of the p15(INK4b) gene promoter in leukemias, and it has been proposed that this methylation could be necessary for leukemic cells...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1998-04-15 00:00:00
abstract：:Deregulation of signal transduction pathways (STPs) may promote leukemogenesis by conferring cell proliferation and survival advantages in acute myelogenous leukemia (AML). Several agents targeting STPs are under development; however, redundancy and cross-talk between STPs could activate multiple downstream effectors ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2006-02-003475
更新日期：2006-10-01 00:00:00
abstract：:The bcr1- and bcr3- promyelocytic leukemia/retinoic acid receptor alpha (PML/RAR alpha) are the two major fusion proteins expressed in acute promyelocytic leukemia (APL) patients. These proteins, which are present in different lengths of PML (amino acids 1-552 and 1-394, respectively), contain most of the functional d...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
abstract：:Leukemia relapse is a major cause of treatment failure for patients with acute leukemia given allogeneic bone marrow transplantation (BMT). This study evaluated whether a reduction of the dosage of cyclosporine-A (Cs-A) used for graft versus host disease (GVHD) prophylaxis could reduce relapse rate (RR) in children wi...
journal_title：Blood
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：
更新日期：2000-03-01 00:00:00
abstract：:Two families of Epstein-Barr virus (EBV), type A and type B, have been defined on the basis of sequence divergence in the EBNA-2 gene. Type A EBV immortalizes B cells more efficiently in vitro and infects immunocompetent individuals more commonly than type B EBV. However, increased rates of infection by type B EBV are...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：:Parasitic worms of the genus Schistosoma excrete relatively large amounts of immunogenic glycoproteins (circulating cathodic antigen [CCA]) that contain polysaccharide side chains with the trisaccharide Lewis-x (L(ex)) as a repeating unit. These carbohydrates evoke high titers of specific IgM antibodies that cross-rea...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:Transplant-associated thrombotic microangiopathy (TA-TMA) occurs frequently after hematopoietic stem cell transplantation (HSCT) and can lead to significant morbidity and mortality. There are no data addressing individual susceptibility to TA-TMA. We performed a hypothesis-driven analysis of 17 candidate genes known t...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2015-08-663435
更新日期：2016-02-25 00:00:00
abstract：:The most common chromosomal abnormality of infant acute lymphoblastic leukemia (ALL) is the t(4;11)(q21;q23) that gives rise to the MLL/AF4 fusion gene. Leukemic blasts expressing MLL/AF4 are arrested at an early progenitor stage with lymphoid or monocytoid characteristics. A novel B-lineage ALL cell line termed B-lin...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood.v98.12.3398
更新日期：2001-12-01 00:00:00
abstract：:Despite the success of human leukocyte antigen (HLA) typing in allogeneic stem cell transplantation (SCT) it is rare to find an unrelated donor that is perfectly matched, making identification of "permissive" mismatches of paramount importance. Here, we describe novel associations between donor T-cell cytokine product...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2003-01-0192
更新日期：2004-01-15 00:00:00
abstract：:Macrophage colony-stimulating factor (M-CSF or CSF-1) and granulocyte-macrophage CSF (GM-CSF) have been shown to increase human monocyte urokinase-type plasminogen-activator (u-PA) activity with possible consequences for cell migration and tissue remodeling; because monocyte u-PA activity is likely to be controlled in...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1993-12-15 00:00:00
abstract：:We used in vitro DNA amplification by the polymerase chain reaction and nonradioactive probes for prenatal diagnosis of beta thalassemia in Chinese from the Guangdong province. Exact molecular diagnoses were made in all 20 fetuses studied over a 6-month period. We conclude that this method of prenatal diagnosis for be...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1989-02-01 00:00:00
abstract：:The molecular backgrounds of variants encountered in Afro-Caribbean black individuals and associated with the production of clinically significant antibodies against high-incidence antigens (anti-RH18, anti-RH34) and against Rhe epitopes were determined. We showed that RH:-18 phenotypes are produced by 3 distinct RHCE...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2002-01-0229
更新日期：2002-12-01 00:00:00
abstract：:The B-cell leukemia/lymphoma-2 (BCL-2) family of proteins governs the intrinsic pathway of mitochondrial apoptosis. Dysregulation of BCL-2 has long been known to be a crucial part of the pathophysiology of B-cell lymphomas; however, several early attempts to target this pathway therapeutically were unsuccessful becaus...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2017-04-737338
更新日期：2017-08-31 00:00:00
abstract：:Circulating monocytes can differentiate into dendritic cells (moDCs), which are potent inducers of adaptive immune responses. Previous reports show that granulocyte macrophage-colony-stimulating factor (GM-CSF) and interleukin-4 induce monocyte differentiation into moDCs in vitro, but little is known about the physiol...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2007-02-076364
更新日期：2007-10-01 00:00:00
