Lack of response of INT-407 cells to the presence of non-culturable Campylobacter jejuni.

Abstract:

:Many contradictory articles on the infectivity of non-culturable Campylobacter jejuni can be found. We studied the effect of non-culturable C. jejuni in an in vitro assay. To prevent the potential effect of a few culturable bacteria in the non-culturable suspension, INT-407 cells, which mimic the outer cell layer in the small intestines, were exposed to culturable C. jejuni suspensions with or without non-culturable C. jejuni. The number of bacteria adhering to and/or invading INT-407 cells and the IL-8 secretion were measured. No differences were found between bacterial suspensions with or without non-culturable C. jejuni added. These findings show that non-culturable C. jejuni do not adhere to or invade INT-407 cells and do not induce an immune response. As previous studies showed a correlation between the used in vitro assays and the effect in vivo, our study strongly suggests that culturability is a good indicator of the risk for C. jejuni infection.

journal_name

Epidemiol Infect

authors

Verhoeff-Bakkenes L,Hazeleger WC,Zwietering MH,De Jonge R

doi

10.1017/S0950268807000040

subject

Has Abstract

pub_date

2008-10-01 00:00:00

pages

1401-6

issue

10

eissn

0950-2688

issn

1469-4409

pii

S0950268807000040

journal_volume

136

pub_type

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