Abstract:
:Among dendritic cell (DC) subsets, CD8alpha(+) DCs and plasmacytoid DCs (pDCs) produce high levels of IL12 and type I interferons (IFNs), respectively, and confer early innate immunity. Development of CD8alpha(+) DCs and pDCs requires the interferon regulatory factor 8 (IRF8). Recently, a spontaneous point mutation was identified in the Irf8/Icsbp gene in the BXH2 mouse, which exhibits an immunodeficient phenotype similar to the IRF8 knockout (KO) mouse. We show that this mutation, designated IRF8(R294C), abolishes the development of CD8alpha(+) DCs without impairing pDC development, and eliminates production of IL12p40, while retaining that of type I IFNs. Electrophoretic mobility shift and chromatin immunoprecipitation assays indicated that IRF8(R294C) failed to interact with partner transcription factors and did not bind certain promoters that require partner interactions. Together, this work indicates that IRF8-partner interactions play different roles in CD8alpha(+) DCs and pDCs, revealing a mechanistic separation that underlies development of these DC subsets.
journal_name
Bloodjournal_title
Bloodauthors
Tailor P,Tamura T,Morse HC 3rd,Ozato Kdoi
10.1182/blood-2007-07-100750subject
Has Abstractpub_date
2008-02-15 00:00:00pages
1942-5issue
4eissn
0006-4971issn
1528-0020pii
blood-2007-07-100750journal_volume
111pub_type
杂志文章相关文献
BLOOD文献大全abstract::Intrachromosomal amplification of chromosome 21 (iAMP21) defines a distinct subgroup of childhood B-cell precursor acute lymphoblastic leukemia (BCP-ALL) that has a dismal outcome when treated with standard therapy. For improved diagnosis and risk stratification, the initiating genetic events need to be elucidated. To...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-01-329961
更新日期:2011-06-23 00:00:00
abstract::CD99 is a crucial regulator of the transmigration (diapedesis) of leukocytes through the blood vessel wall. Here, we report that CD99 acts at 2 different steps in the extravasation process. In agreement with previous antibody-blocking experiments, we found that CD99 gene inactivation caused neutrophil accumulation bet...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2016-08-733394
更新日期:2017-03-30 00:00:00
abstract::The three-dimensional tertiary structure of human von Willebrand Factor (vWF) on a hydrophobic surface under aqueous conditions and different shear stress regimes was studied by atomic force microscopy (AFM). vWF was imaged by AFM at molecular level resolution under negligible shear stress, under a local applied shear...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-10-15 00:00:00
abstract::We used our recently reported stable, transformed human renal carcinoma cell line as a model system to study the role of 3',5'-adenosine monophosphate (cAMP) in erythropoietin secretion. The erythropoietin produced by these cells is both biologically active and immunologically cross-reactive with purified native human...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1987-04-01 00:00:00
abstract::Autologous stem cell transplantation (ASCT) is considered the gold standard in the frontline therapy of younger patients with multiple myeloma because it results in higher complete remission (CR) rates and longer event-free survival than conventional chemotherapy. The greatest benefit from ASCT is obtained in patients...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2009-08-238196
更新日期:2010-05-06 00:00:00
abstract::The efficacy and safety of zanolimumab in patients with refractory cutaneous T-cell lymphoma (CTCL) have been assessed in two phase 2, multicenter, prospective, open-label, uncontrolled clinical studies. Patients with treatment refractory CD4(+) CTCL (mycosis fungoides [MF], n = 38; Sézary syndrome [SS], n = 9) receiv...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:10.1182/blood-2006-12-062877
更新日期:2007-06-01 00:00:00
abstract::We studied the efficiency of indirect tumor cell purging via enrichment of CD34+ hematopoietic progenitor cells from leukapheresis products (LP) in breast cancer patients based on immunomagnetic selection of CD34+ cells. Detection of tumor cells was made by immunocytochemical staining. In addition, we evaluated the ca...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::At the intersection between children and older adults, the care of adolescent and young adult (AYA) patients with acute lymphoblastic leukemia (ALL) poses unique challenges and issues beyond those faced by other age groups. Although the survival of AYA patients is inferior to younger children, growing evidence suggest...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2014-11-551481
更新日期:2015-06-11 00:00:00
abstract::Recently (J Pharmacol Exp Ther 261:580, 1992), we have shown that K562 leukemia cells express a calcium-signaling purinoceptor with characteristics of the P2T receptor subtype for adenosine diphosphate (ADP) previously found only in platelets. Because these results suggested that the P2T receptor may be an early marke...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::The etiopathologic basis of painful crisis in sickle cell anemia is largely unknown, and no objective criteria for its diagnosis and follow-up exist at present. We have studied 11 patients through 14 painful crises and observed a significant decrease of the densest fraction of red cells in 12 of the 14 crises as deter...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-08-01 00:00:00
abstract::Contraction of blood clots is necessary for hemostasis and wound healing and to restore flow past obstructive thrombi, but little is known about the structure of contracted clots or the role of erythrocytes in contraction. We found that contracted blood clots develop a remarkable structure, with a meshwork of fibrin a...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-08-523860
更新日期:2014-03-06 00:00:00
abstract::Approximately 50% of multi-transfused individuals become refractory to random donor platelets. Recent clinical data suggest that those patients receiving leukocyte-depleted blood products are less likely to become refractory to random donor platelets than recipients of non-leukocyte-depleted products. Leukocyte deplet...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1992-03-01 00:00:00
abstract::Targeting cancer stem cells is of paramount importance in successfully preventing cancer relapse. Recently, in silico screening of public gene-expression datasets identified cyclooxygenase-derived cyclopentenone prostaglandins (CyPGs) as likely agents to target malignant stem cells. We show here that Δ(12)-PGJ(3), a n...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-11-317750
更新日期:2011-12-22 00:00:00
abstract::A new combination of total lymphoid irradiation and cyclophosphamide was used prior to bone marrow transplantation in an attempt to achieve decreased rejection rates and graft-versus-host disease. Nine previously transfused patients with severe aplastic anemia received marrow from an HLA-identical, MLC-compatible sibl...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1980-02-01 00:00:00
abstract::Natural killer cells (NK) were studied to determine factors important in their expansion. Flourescence-activated cell sorter (FACS) purified CD56+/CD3- NK cells cultured alone for 18 days in rIL-2 containing medium (1,000 U/mL) showed enhanced cytotoxicity but only minimal expansion. NK expansion was increased (12.5 +...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::We have identified a small molecular weight compound, SCH 14988, which specifically stimulates in vitro granulocyte-colony stimulating factor (G-CSF) production from activated human peripheral blood mononuclear cells and monocytes but not other cytokines or CSFs with hematoregulatory activity. In vivo administration o...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-07-15 00:00:00
abstract::Nucleophosmin-mutated acute myeloid leukemia (NPM1mut-AML) patients have a high rate of complete remission (CR) to induction chemotherapy. However, the mechanisms responsible for such effects are unknown. Because miR-10 family members are expressed at high levels in NPM1mut-AML, we evaluated whether these microRNAs co...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-10-532374
更新日期:2014-04-10 00:00:00
abstract::The Kell blood group is one of the major antigenic systems in human red blood cells. To determine the location of the Kell gene on human chromosomes, panels containing genomic DNA of human-hamster somatic cell hybrids were hybridized with radiolabeled cDNA probe specific for the Kell locus. Only the samples containing...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-05-15 00:00:00
abstract::The ability to determine the functional capacity of putative human hematopoietic stem cell (HSC) populations requires in vivo assays in which long-term multilineage differentiation can be assessed. We hypothesized that if human fetal bone was transplanted adjacent to a fetal thymus fragment in severe combined immunode...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::Clinical resistance to imatinib mesylate is commonly observed in patients with advanced Philadelphia chromosome- positive (Ph(+)) leukemias. Acquired resistance is typically associated with reactivation of BCR-ABL due to kinase domain mutations or gene amplification, indicating that BCR-ABL remains a viable target for...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-05-1361
更新日期:2002-10-15 00:00:00
abstract::Recent findings that hemoglobin S gelation and sickling are pH-dependent and also influence oxygen affinity suggested that the red cells containing this hemoglobin variant might show an abnormal Bohr effect. We therefore studied the effects of pH variation on the in vitro oxygen affinity of whole blood from persons wi...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1979-03-01 00:00:00
abstract::Although the binding of endothelial cell protein C receptor (EPCR) to its ligands is well characterized at the biochemical level, it remains unclear how EPCR interaction with its ligands at the cell surface impacts its cellular trafficking. We characterized the cellular localization and trafficking of EPCR in endothel...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-03-208900
更新日期:2009-08-27 00:00:00
abstract::The main interface of the 2 subunits of platelet integrin alphaIIbbeta3 comprises the beta-propeller domain of alphaIIb and the betaA domain of beta3. In the center of the beta-propeller, several aromatic residues interact by cation-pi and hydrophobic bonds with Arg261 of betaA. In this study, we substituted alphaIIb-...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-10-251066
更新日期:2010-06-03 00:00:00
abstract::It has now been 3 years since the von Willebrand factor (VWF)-cleaving protease implicated in thrombocytopenic purpura (TTP) pathogenesis was identified as ADAMTS13 (a disintegrin-like and metalloprotease with thrombospondin type 1 motif 13). More than 50 ADAMTS13 mutations resulting in familial TTP have been reported...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2004-10-4097
更新日期:2005-07-01 00:00:00
abstract::Essential thrombocythemia (ET) and primary myelofibrosis (PMF) are chronic diseases characterized by clonal hematopoiesis and hyperproliferation of terminally differentiated myeloid cells. The disease is driven by somatic mutations in exon 9 of CALR or exon 10 of MPL or JAK2-V617F in >90% of the cases, whereas the rem...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-07-661835
更新日期:2016-01-21 00:00:00
abstract::A 9-yr-old female presented with a Wilm's tumor and a coagulopathy consistent with von Willebrand's disease. Factor VIII procoagulant activity (VIII C), factor VIII related antigen (VIIIR:Ag), and von Willebrand factor activity (VIII:vWf) were decreased. There was no evidence for a circulating inhibitor of the factor ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-10-01 00:00:00
abstract::Targeting mechanisms of neutrophil elastase (NE) and other luminal proteins stored in myeloperoxidase (MPO)-positive secretory lysosomes/primary granules of neutrophils are unknown. These granules contain an integral membrane protein, CD63, with an adaptor protein-3-dependent granule delivery system. Therefore, we hyp...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-10-116285
更新日期:2008-10-15 00:00:00
abstract::Pregnancy represents a major challenge to immunologic tolerance. How the fetal "semiallograft" evades maternal immune attack is unknown. Pregnancy success may involve alteration of both central (thymic) and peripheral tolerance mechanisms. HIV infection is characterized by CD4(+) T-cell depletion, chronic immune activ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-07-298992
更新日期:2011-02-10 00:00:00
abstract::In a series of 153 children with T-cell malignancies enrolled in 2 consecutive European Organization for Research and Treatment of Cancer (EORTC) trials, we assessed the HOX11L2 expression and/or the presence of a t(5;14)(q35;q32). Additionally, in 138 of these patients, HOX11 expression and SIL-TAL rearrangement were...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:10.1182/blood-2003-05-1495
更新日期:2004-01-15 00:00:00
abstract::Productive T-cell immunity requires both the activation and the migration of specific T cells to the antigenic tissue. The costimulatory molecule CD28 plays an essential role in the initiation of T-cell-mediated immunity. We investigated the possibility that CD28 may also regulate migration of primed T cells to target...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-10-050724
更新日期:2007-04-01 00:00:00