Abstract:
:Contraction of blood clots is necessary for hemostasis and wound healing and to restore flow past obstructive thrombi, but little is known about the structure of contracted clots or the role of erythrocytes in contraction. We found that contracted blood clots develop a remarkable structure, with a meshwork of fibrin and platelet aggregates on the exterior of the clot and a close-packed, tessellated array of compressed polyhedral erythrocytes within. The same results were obtained after initiation of clotting with various activators and also with clots from reconstituted human blood and mouse blood. Such close-packed arrays of polyhedral erythrocytes, or polyhedrocytes, were also observed in human arterial thrombi taken from patients. The mechanical nature of this shape change was confirmed by polyhedrocyte formation from the forces of centrifugation of blood without clotting. Platelets (with their cytoskeletal motility proteins) and fibrin(ogen) (as the substrate bridging platelets for contraction) are required to generate the forces necessary to segregate platelets/fibrin from erythrocytes and to compress erythrocytes into a tightly packed array. These results demonstrate how contracted clots form an impermeable barrier important for hemostasis and wound healing and help explain how fibrinolysis is greatly retarded as clots contract.
journal_name
Bloodjournal_title
Bloodauthors
Cines DB,Lebedeva T,Nagaswami C,Hayes V,Massefski W,Litvinov RI,Rauova L,Lowery TJ,Weisel JWdoi
10.1182/blood-2013-08-523860subject
Has Abstractpub_date
2014-03-06 00:00:00pages
1596-603issue
10eissn
0006-4971issn
1528-0020pii
blood-2013-08-523860journal_volume
123pub_type
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