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Antigen-specific CD8+ T cells induced by the ubiquitin fusion degradation pathway.

Abstract：

:We have developed a DNA vaccine encoding a fusion protein of ubiquitin (Ub) and target proteins at the N-terminus for effective induction of antigen-specific CD8(+) T cells. A series of expression plasmids encoding a model antigen, ovalbumin (OVA), fused with mutated Ub, was constructed. Western blotting analyses using COS7 cells transfected with these plasmids revealed that there were three types of amino acid causing different binding capacities between Ub and OVA. Natural Ub with a C-terminal glycine readily dissociated from OVA; on the other hand, artificially mutated Ub, the C-terminal amino acid of which had been exchanged to valine or arginine, stably united with the polypeptide, while Ub with a C-terminal alanine partially dissociated. The ability of DNA vaccination to induce OVA-specific CD8(+) T cells closely correlated with the stability of Ub fusion to OVA. Our strategy could be used to optimize the effect of genetic vaccines on the induction of CD8(+) T cells.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Imai T,Duan X,Hisaeda H,Himeno K

doi

10.1016/j.bbrc.2007.11.034

subject

Has Abstract

pub_date

2008-01-25 00:00:00

pages

758-63

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(07)02445-X

journal_volume

365

pub_type

杂志文章

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