Using pCIN-mIL-4 DNA vector to express mRNA and protein and to improve herpes simplex virus-induced Behcet's disease symptoms in mice.


:Behcet's disease (BD) is a chronic, recurrent, inflammatory, multisystemic disorder characterized primarily by vasculitis. The etiopathogenesis of BD involves immunogenetics, infectious organisms (streptococcus, herpes simplex virus), immunoregulation and vascular dysfunctions. We previously found that immunoregulation associated with viral infection was important to the development of BD-like symptoms. Recently, we demonstrated that Th2 cytokines up-regulated by Th2 adjuvant were efficient in attenuating or improving these BD-like symptoms. In order to directly augment IL-4 expression, a DNA vector (pCIN-mIL-4) was administered to BD-like mice using the Helios gene gun system. Two injections of the pCIN-mIL-4 vector, spread over 2 weeks, attenuated or improved the mucocutaneous symptoms of 10 out of 12 BD-like mice in our study. The improved mucocutaneous symptoms were crust in face, ulcer in mouth, scruff, back, genital and erythema. This improvement also correlated with induction of IL-4 mRNA in lymph nodes, protein in serum and intracellular IL-4 staining in splenocytes. Normal control mice (n = 10) injected with the pCIN-mIL-4 vector expressed IL-4 mRNA and showed more splenocytes stained with anti-IL-4 antibody (5.77 +/- 0.92%) than did mice injected with the pCIN control vector (3.34 +/- 0.25%; p = 0.02). These findings indicate that an IL-4 DNA vector could be used to express mRNA and protein in vivo and further suggest that such an IL-4 DNA vector could be used as a therapeutic treatment in recurrent inflammation shifted to T helper type 1 cytokine production.






Lee SI,Kwon HJ,Lee ES,Yang BC,Bang D,Lee S,Sohn S




Has Abstract


2007-10-10 00:00:00
















  • Technological investigations with attenuated strains of Shigella for production of live vaccines. 1. Cultivation in a fermenter of an attenuated strain S. flexneri 2a with two markers.

    abstract::A method was developed for cultivation of the strain Shigella flexneri 2a 77 with two attenuated markers. It ensures the preservation of the initial properties of the strain. The regimen for control of dissolved oxygen level was optimized. It was established that yeast extract is a necessary component of the nutritive...


    pub_type: 杂志文章


    authors: Nenkov P,Bratoeva M,Vitanov T,Marinova S,Denchev V,Linde K

    更新日期:1987-09-01 00:00:00

  • Provider time and costs to vaccinate adult patients: Impact of time counseling without vaccination.

    abstract::Amid provider reports of financial barriers as an impediment to adult immunization, this study explores the time and costs of vaccination in adult provider practices. Both a Vaccination Time-Motion Study and Vaccine Practice Management Survey were conducted (March - October 2017) in a convenience sample of 19 family m...


    pub_type: 杂志文章


    authors: Shen A,Khavjou O,King G,Bates L,Zhou F,Leidner AJ,Yarnoff B

    更新日期:2019-02-04 00:00:00

  • Innate immunemodulator containing adjuvant formulated HA based vaccine protects mice from lethal infection of highly pathogenic avian influenza H5N1 virus.

    abstract::The highly pathogenic avian influenza (HPAI) H5N1 viruses and their spillover into the human population pose substantial economic and public health threats. Although antiviral drugs have some effect in treating influenza infection, vaccination is still the most effective intervention to prevent possible pandemic outbr...


    pub_type: 杂志文章


    authors: Lu Y,Landreth S,Liu G,Brownlie R,Gaba A,Littel-van den Hurk SVD,Gerdts V,Zhou Y

    更新日期:2020-02-28 00:00:00

  • Serological and epidemiological effects and influence factors of primary immunization with current live attenuated measles vaccine (Hu191) among infants aged 6-15 months.

    abstract::A study of the serological and epidemiological effects of primary immunization with current live attenuated measles vaccine (Hu191) among 503 children aged 6-15 months, was conducted in Jingzhou, Hubei province, China from 1991 to 1998. The results showed that the positive conversion rate of measles IgG was 91.65%, ge...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Youwang Y,Ping W,Feng C

    更新日期:2001-02-28 00:00:00

  • The tuberculosis vaccine H4:IC31 is safe and induces a persistent polyfunctional CD4 T cell response in South African adults: A randomized controlled trial.

    abstract:BACKGROUND:New, more effective vaccines to prevent tuberculosis (TB) disease are needed urgently. H4:IC31 is an investigational vaccine that contains a fusion protein of the immunodominant antigens TB10.4 and Ag85B, formulated in IC31 adjuvant. We assessed the safety and immunogenicity of H4:IC31 in South African adult...


    pub_type: 杂志文章,随机对照试验


    authors: Geldenhuys H,Mearns H,Miles DJ,Tameris M,Hokey D,Shi Z,Bennett S,Andersen P,Kromann I,Hoff ST,Hanekom WA,Mahomed H,Hatherill M,Scriba TJ,H4:IC31 Trial Study Group.,van Rooyen M,Bruce McClain J,Ryall R,de Bruyn G,H4:

    更新日期:2015-07-09 00:00:00

  • Comparison of NITAG policies and working processes in selected developed countries.

    abstract:BACKGROUND:Vaccines are specific medicines characterized by two country-specific market access processes: (1) a recommendation by National Immunization Technical Advisory Group (NITAG), and (2) a funding policy decision. OBJECTIVES:The objective of this study was to compare and analyze NITAGs of 13 developed countries...


    pub_type: 杂志文章


    authors: Ricciardi GW,Toumi M,Weil-Olivier C,Ruitenberg EJ,Dankó D,Duru G,Picazo J,Zöllner Y,Poland G,Drummond M

    更新日期:2015-01-01 00:00:00

  • Characterization of a multi-lipopeptides mixture used as an HIV-1 vaccine candidate.

    abstract::A multi-component vaccine has been defined, which contains six different synthetic 24- to 32-amino acid lipopeptides derived from the sequence of HIV-1 proteins. The physicochemical properties of the lipopeptide components were compatible with multi-dimensional analysis, using RP-HPLC, Edman sequencing, electrospray m...


    pub_type: 杂志文章


    authors: Klinguer C,David D,Kouach M,Wieruszeski JM,Tartar A,Marzin D,Levy JP,Gras-Masse H

    更新日期:1999-09-01 00:00:00

  • Cytokine production patterns and antibody response to measles vaccine.

    abstract::Cytokines play an important role in the immune response to live measles virus immunization. To gain further insight into the cytokine production profile in response to measles vaccination, we studied interferon-gamma (IFN-gamma), tumor necrosis factor (TNF-alpha), soluble IL-2 receptor (sIL-2R), interleukin-2 (IL-2), ...


    pub_type: 临床试验,杂志文章


    authors: Ovsyannikova IG,Reid KC,Jacobson RM,Oberg AL,Klee GG,Poland GA

    更新日期:2003-09-08 00:00:00

  • Heterogeneous early immune responses to the S. aureus EapH2 antigen induced by gastrointestinal tract colonisation impact the response to subsequent vaccination.

    abstract:INTRODUCTION:S. aureus is a pathogen to which individuals are exposed shortly after birth, with immune responses to S. aureus increasing during childhood. There is marked heterogeneity between the anti- S. aureus immune responses of different humans, the basis of which is not fully understood. METHODS:To investigate d...


    pub_type: 杂志文章


    authors: Flaxman A,Yamaguchi Y,van Diemen PM,Rollier C,Allen E,Elshina E,Wyllie DH

    更新日期:2019-01-14 00:00:00

  • Comparative biological activities of whole cell pertussis vaccine and a new acellular preparation.

    abstract::The biological activities of whole-cell pertussis vaccine and a new acellular preparation were compared. It was observed that both preparations were similarly effective in inducing protection in mice against intracerebral challenge with B. pertussis, however, the acellular preparation was approximately 200-fold more p...


    pub_type: 临床试验,杂志文章


    authors: García-Sánz JA,Ruiz-Puente J,Jiménez-Paredes J,González-Pacheco M,Villalva-Posada H

    更新日期:1985-03-01 00:00:00

  • Clearing asymptomatic parasitaemia increases the specificity of the definition of mild febrile malaria.

    abstract::In clinical trials, the specificity of the disease endpoint is critical to an accurate estimate of vaccine efficacy. We used a logistic regression model to analyse parasite densities among children before and after treatment with antimalarials, in order to estimate the impact that clearing asymptomatic parasitaemia ha...


    pub_type: 杂志文章


    authors: Bejon P,Mwangi T,Lowe B,Peshu N,Hill AV,Marsh K

    更新日期:2007-11-23 00:00:00

  • Potentiation by a novel alkaloid glycoside adjuvant of a protective cytotoxic T cell immune response specific for a preerythrocytic malaria vaccine candidate antigen.

    abstract::We have recently demonstrated that the novel glycoalkaloid tomatine, derived from leaves of the wild tomato Lycopersicon pimpinellifolium, can act as a powerful adjuvant for the elicitation of antigen-specific CD8+ T cell responses. Here, we have extended our previous investigation with the model antigen ovalbumin to ...


    pub_type: 杂志文章


    authors: Heal KG,Sheikh NA,Hollingdale MR,Morrow WJ,Taylor-Robinson AW

    更新日期:2001-07-20 00:00:00

  • Development pathway for biodefense vaccines.

    abstract::At the present time it is estimated that the process of development of a vaccine from discovery to licensure takes approximately 18-20 years and costs in excess of US$500 million. For "routine" vaccines, the case for developing a vaccine is straightforward in terms of economics and large scale public health utilizatio...


    pub_type: 杂志文章,评审


    authors: Barrett AD,Beasley DW

    更新日期:2009-11-05 00:00:00

  • Eimeria species parasites as novel vaccine delivery vectors: anti-Campylobacter jejuni protective immunity induced by Eimeria tenella-delivered CjaA.

    abstract::Vaccination of poultry against coccidiosis caused by the Eimeria species is almost entirely based upon varied formulations of live parasites. The recent development of a series of protocols that support genetic complementation by transfection in Eimeria now provides an opportunity to utilise live anticoccidial vaccine...


    pub_type: 杂志文章


    authors: Clark JD,Oakes RD,Redhead K,Crouch CF,Francis MJ,Tomley FM,Blake DP

    更新日期:2012-03-30 00:00:00

  • Vaccine-induced intestinal immunity to ricin toxin in the absence of secretory IgA.

    abstract::The RNA N-glycosidase ribosome inactivating proteins (RIPs) constitute a ubiquitous family of plant- and bacterium-derived toxins that includes the category B select agents ricin, abrin and shiga toxin. While these toxins are potent inducers of intestinal epithelial cell death and inflammation, very little is known ab...


    pub_type: 杂志文章


    authors: Neal LM,McCarthy EA,Morris CR,Mantis NJ

    更新日期:2011-01-17 00:00:00

  • Delayed progression of murine AIDS in C57BL/6 mice pre-immunized with a highly antigenic 10-mer peptide encoded by the murine AIDS defective virus gag p12 gene.

    abstract::C57BL/6 (B6) mice were immunized with a highly antigenic 10-mer peptide (P12-10), which is encoded by the murine AIDS (MAIDS) defective virus gag p12 gene, emulsified in incomplete Freund's adjuvant (ICFA). One week later, the mice were inoculated with the MAIDS virus to see if the immunization affects progression of ...


    pub_type: 杂志文章


    authors: Mizuochi T,Horino A,Uchida T

    更新日期:1998-12-01 00:00:00

  • Do parents prefer inactivated or live attenuated influenza vaccine for their children?

    abstract:OBJECTIVES:To determine the proportion of children whose parents prefer them to receive live, attenuated influenza vaccine (LAIV) or inactivated influenza vaccine (IIV), examine reasons for preferences, and determine what percentage of vaccinated children receive other than the preferred type of vaccine and why. METHO...


    pub_type: 杂志文章


    authors: Santibanez TA,Kahn KE,Bridges CB

    更新日期:2018-11-19 00:00:00

  • The purification and protective capacity of Bordetella pertussis outer membrane proteins.

    abstract::The whole cell vaccine (WCV) of Bordetella pertussis is protective in the intracerebral (i.c.) mouse protection assay. We found a correlation between the i.c. mouse protection assay potency and the presence of the virulence-associated outer membrane proteins (OMPs) in outer membrane complexes (OMC). The virulence-asso...


    pub_type: 杂志文章


    authors: Hamstra HJ,Kuipers B,Schijf-Evers D,Loggen HG,Poolman JT

    更新日期:1995-01-01 00:00:00

  • Defining hard-to-reach populations for vaccination.

    abstract::Extending the benefits of vaccination to everyone who is eligible requires an understanding of which populations current vaccination efforts have struggled to reach. A clear definition of "hard-to-reach" populations - also known as high-risk or marginalized populations, or reaching the last mile - is essential for est...


    pub_type: 杂志文章,评审


    authors: Ozawa S,Yemeke TT,Evans DR,Pallas SE,Wallace AS,Lee BY

    更新日期:2019-09-03 00:00:00

  • Induction of autoantibody production but not autoimmune disease in HEL transgenic mice vaccinated with HEL in combination with CpG or control oligodeoxynucleotides.

    abstract::CpG oligodeoxynucleotides (ODN) are synthetic DNA sequences that mimic bacterial DNA, and bind to the TLR9 receptor. The cells that express TLR9, B cells and dendritic cells, are stimulated by CpG ODN and induce innate and acquired immune responses. Because CpG ODN induce antigen-independent immune activation there ha...


    pub_type: 杂志文章


    authors: Wang Y,Krieg AM

    更新日期:2004-06-30 00:00:00

  • Humoral immunity 5 years after booster immunization with an adolescent and adult formulation combined tetanus, diphtheria, and 5-component acellular pertussis vaccine.

    abstract::Persistence of antibodies following a single dose of Tdap vaccine (tetanus, diphtheria, and five-component acellular pertussis vaccine for use in individuals past childhood) was evaluated in a follow-up of adolescents (N=324) and adults (N=644) who had received Tdap in earlier clinical trials. Outcome measures were se...


    pub_type: 杂志文章


    authors: Barreto L,Guasparini R,Meekison W,Noya F,Young L,Mills E

    更新日期:2007-11-23 00:00:00

  • Influenza vaccination rates in Ontario children: implications for universal childhood vaccination policy.

    abstract::The aims of this study were to estimate influenza vaccination coverage for children during the 2006-2007 influenza season in Ontario, Canada, where universal vaccination is available, and to compare the rate among children aged 6-23 months with corresponding rates from other Canadian provinces that specifically target...


    pub_type: 杂志文章


    authors: Moran K,Maaten S,Guttmann A,Northrup D,Kwong JC

    更新日期:2009-04-14 00:00:00

  • Elucidation of the full genetic information of Japanese rubella vaccines and the genetic changes associated with in vitro and in vivo vaccine virus phenotypes.

    abstract::Rubella is a mild disease characterized by low-grade fever, and a morbilliform rash, but causes congenital defects in neonates born from mothers who suffered from rubella during the pregnancy. After many passages of wild-type rubella virus strains in various types of cultured cells, five live attenuated rubella vaccin...


    pub_type: 杂志文章


    authors: Otsuki N,Abo H,Kubota T,Mori Y,Umino Y,Okamoto K,Takeda M,Komase K

    更新日期:2011-02-24 00:00:00

  • Lower peripheral blood CD14+ monocyte frequency and higher CD34+ progenitor cell frequency are associated with HBV vaccine induced response in HIV infected individuals.

    abstract::We evaluated immunologic predictors of response to HBV vaccine administered in the presence or absence of GM-CSF in HIV infected individuals. We measured peripheral blood hematopoietic progenitor, monocyte and myeloid-derived suppressor cell (MDSC) frequencies, and expression of GMCSF receptor on monocytes and MDSCs, ...


    pub_type: 杂志文章,随机对照试验


    authors: Anthony DD,Umbleja T,Aberg JA,Kang M,Medvik K,Lederman MM,Peters MG,Koziel MJ,Overton ET

    更新日期:2011-04-27 00:00:00

  • A plant-produced vaccine protects mice against lethal West Nile virus infection without enhancing Zika or dengue virus infectivity.

    abstract::West Nile virus (WNV) has caused multiple global outbreaks with increased frequency of neuroinvasive disease in recent years. Despite many years of research, there are no licensed therapeutics or vaccines available for human use. One of the major impediments of vaccine development against WNV is the potential enhancem...


    pub_type: 杂志文章


    authors: Lai H,Paul AM,Sun H,He J,Yang M,Bai F,Chen Q

    更新日期:2018-03-27 00:00:00

  • Changes in Streptococcus pneumoniae serotype distribution in invasive disease and nasopharyngeal carriage after the heptavalent pneumococcal conjugate vaccine introduction in Bogotá, Colombia.

    abstract:BACKGROUND:In Bogotá, the Heptavalent Conjugate Vaccine (PCV7) was introduced into childhood immunization schedule since 2009. The aim of this study was to assess the changes in serotype distribution and penicillin susceptibility of Streptococcus pneumoniae isolates recovered from nasopharyngeal samples and invasive di...


    pub_type: 杂志文章


    authors: Parra EL,De La Hoz F,Díaz PL,Sanabria O,Realpe ME,Moreno J

    更新日期:2013-08-20 00:00:00

  • Recombinant H7 hemagglutinin forms subviral particles that protect mice and ferrets from challenge with H7N9 influenza virus.

    abstract::A novel avian-origin influenza A H7N9 virus emerged in China in 2013 and continues to cause sporadic human infections with mortality rates approaching 35%. Currently there are no approved human vaccines for H7N9 virus. Recombinant approaches including hemagglutinin (HA) and virus-like particles (VLPs) have resulted in...


    pub_type: 杂志文章


    authors: Pushko P,Pujanauski LM,Sun X,Pearce M,Hidajat R,Kort T,Schwartzman LM,Tretyakova I,Chunqing L,Taubenberger JK,Tumpey TM

    更新日期:2015-09-11 00:00:00

  • Adjuvants influence the quantitative and qualitative immune response in BALB/c mice immunized with respiratory syncytial virus FG subunit vaccine.

    abstract::The ability of monophosphoryl lipid A (MPL), QS-21 and alum to alter the immunologic response to immunization with respiratory syncytial virus a chimeric FG construct (FG) subunit vaccine was examined in BALB/c mice. FG/MPL, FG/alum, and FG/MPL/QS-21 combinations increased non-neutralizing antibody response, while FG/...


    pub_type: 杂志文章


    authors: Neuzil KM,Johnson JE,Tang YW,Prieels JP,Slaoui M,Gar N,Graham BS

    更新日期:1997-04-01 00:00:00

  • A randomized, open label trial to evaluate and compare the immunogenicity and safety of a novel liquid hexavalent DTwP-Hib/Hep B-IPV (EasySix™) to licensed combination vaccines in healthy infants.

    abstract::Immunogenicity and safety of a newly developed liquid DTwP-Hib/HepB-IPV hexavalent vaccine (EasySix™) was evaluated and compared with administration of commercially licensed Pentavac SD® (DTwP-HepB/Hib) and Imovax Polio® vaccine in an open-label, randomized multi-centric trial. 284 participants, aged 6-10weeks, random...


    pub_type: 杂志文章,随机对照试验


    authors: Mohanty L,Sharma S,Behera B,Panwar S,Paliwal C,Gupta A,Chilkoti DC,Singh A

    更新日期:2018-04-19 00:00:00

  • Impact of MenBvac, an outer membrane vesicle (OMV) vaccine, on the meningococcal carriage.

    abstract::The aim of the study was to analyze the impact of MenBvac, an outer membrane vesicle (OMV) vaccine against P1.7,16 strains, on meningococcal carriage. During a B:14:P1.7,16/ST-32 outbreak in Normandy (France), children aged 1-7 years were randomly selected to participate in the study. Among the 1082 volunteers, there ...


    pub_type: 杂志文章


    authors: Delbos V,Lemée L,Bénichou J,Berthelot G,Deghmane AE,Leroy JP,Houivet E,Hong E,Taha MK,Caron F,B14 STOP study group.

    更新日期:2013-09-13 00:00:00