Confocal myography for the study of hypertensive vascular remodelling.

Abstract:

:Hypertension is associated with vascular structural alterations known as "vascular remodelling", which initially are adaptive but in the long run, lead to vascular damage and loss of function. Despite decades of study, there is still modest information on the 3-dimensional (3D) arrangement of vascular cells and extracellular matrix (ECM) and how they change under pathological situations. To address this problem we developed a technique which combines fluorescence confocal microscopy, pressure myography and image analysis, "confocal myography", which permits the study of intact resistance-sized vessels at cellular level and at physiological pressure. With the aid of this method, we have identified, in arteries from hypertensive rats, abnormal orientation of endothelial, smooth muscle cells (SMC) and elastic fibres; elongation and denudation of endothelial cells, and adventitial hypercellularity. Confocal myography offers a new approach to the study of vascular remodelling in intact small arteries from a 3D point of view.

authors

Arribas SM,González JM,Briones AM,Somoza B,Daly CJ,Vila E,González MC,McGrath JC

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

205-10

issue

1-2

eissn

1386-0291

issn

1875-8622

journal_volume

37

pub_type

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