Abstract:
:A variety of medical grade polymeric materials are used in tissue engineering and biomedical technology. Dense non-porous polymeric foils were used as substrates for endothelial cell layers. Half a the test samples (polymers and control materials) were seeded with bovine corneal endothelial cells (BCEC) which more or less covered the substrates with an extracellular matrix (ECM) in the consecutive culturing period. Afterwards the ECM covered as well as the uncovered materials were seeded with human umbilical venous endothelial cells (HUVEC). HUVEC seeded samples were cultured either under static or under dynamical conditions in a cone/plate rheometer with a mean low arterial shear stress of 8.2 dyn/cm2 to simulate the flow conditions in a coronary vein graft. With the exemption of polyvinyl chloride all other materials could be coated with ECM at least partially. Under static conditions the best results with respect to complete coverage with ECM and HUVEC were seen on polyester and polyurethane. Under shear load, however, the complete HUVEC layer together with the ECM detached from the polymer surface within a short time. ECM and HUVEC remained no longer than 43 minutes on anyone of the materials tested. The materials as supplied and tested were clearly not appropriate as implants in contact to the flowing blood.
journal_name
Clin Hemorheol Microcircjournal_title
Clinical hemorheology and microcirculationauthors
Hoepken S,Fuhrmann R,Jung F,Franke RPdoi
10.3233/CH-2009-1230subject
Has Abstractpub_date
2009-01-01 00:00:00pages
157-66issue
1-2eissn
1386-0291issn
1875-8622pii
A3415877TG533550journal_volume
43pub_type
杂志文章abstract:PURPOSE:To evaluate the different ultrasound techniques for quantification of contrast enhanced ultrasound (CEUS) in an experimental rat prostate carcinoma model. METHOD AND MATERIALS:Prostate carcinoma (MLLB-2) cells were subcutaneously injected into the right flank of male rat (Charles River, Sulzfeld, Germany, 180 ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2010-1341
更新日期:2010-01-01 00:00:00
abstract::Some studies indicate that obesity is associated with rheological disturbances. Because there are usually sex differences in the type of obesity we decided to evaluate rheological differences between male and female obese patients. We studied 18 morbidly obese men, mean age 43.66 +/- 11.32 years, mean body mass index ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2010-1275
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:Capsular contractures around breast implants usually develop leading to pain and aesthetically inadequate results and ultimately often requires the replacement of the implants. Textured silicone implants are the most commonly placed implant, but polyurethane-coated implants are increasingly being used in an ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-200875
更新日期:2020-09-08 00:00:00
abstract:INTRODUCTION:The hormones erythropoietin (EPO) and thrombopoietin (TPO) are main regulators of erythro- and thrombopoiesis. Cell loss caused by operative procedures may alter serum levels of the hormones, resulting in well known phenomenons like reactice thrombocytosis. MATERIAL AND METHODS:Blood samples from 10 patie...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2011-1489
更新日期:2011-01-01 00:00:00
abstract::Red blood cell distribution width (RDW) has been shown to be associated with disease activity in several inflammatory disorders. However only one study to show this has been conducted in patients with Behçet's disease (BD). The aim of the present study was to analyse the association of RDW with BD and its main complic...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-131744
更新日期:2014-01-01 00:00:00
abstract::Beta-thalassaemia is a hereditary hemolytic disease, in which each clinic phenotype encompasses a heterogenic group of genetic alterations resulting in beta-globin chain synthesis decrease or absence in red blood cells. Studies on beta-thalassaemia carriers suggest the existence of decreased red cell deformability. Th...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::This review focuses on the contribution of abnormal blood rheology in the pathophysiology of sickle cell anemia (SCA). SCA is characterized by a reduction of red blood cell (RBC) deformability but this reduction is very heterogeneous among patients. Recent works have shown that patients with the lowest RBC deformabili...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-189005
更新日期:2018-01-01 00:00:00
abstract::Anemia in pregnant women is associated with increased maternal and perinatal mortality and represents an important economic burden in many developing countries. Our goal was to evaluate the impact of anemia on the capillary network during pregnancy. Therefore, we compared microcirculatory parameters of anemic pregnant...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-162064
更新日期:2016-11-25 00:00:00
abstract:AIM:The aim of the study was to determine the role of some erythrocyte patterns (Red Blood Cell deformability, aggregability, cytosolic calcium concentration) in normal tension glaucoma (NTG), compared with primary open angle glaucoma (POAG) and normal subjects. MATERIAL AND METHODS:Ninety patients entered the study, ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::The concentrations of the acute phase proteins alpha1-Acid Glycoprotein (AAG) and haptoglobin were determined in Sprague-Dawley-rats after implantation of a novel biodegradable multifunctional polymeric biomaterial for the reconstruction of a gastric wall defect (polymer group; n=42). For comparison, the concentration...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Hemorheological and hemodynamic effects of high molecular weight polyethylene oxide (PEO) solutions have been studied in vitro and in vivo at 30 experimental dogs. The rheological behaviour of the PEO solutions and of blood samples has been assessed by rotational viscometers at 20 degrees C, 25 degrees C and 37 degree...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract:AIM:Detection of prostate cancer lesions using transrectal contrast enhanced ultrasound (CEUS) of the prostate utilizing quantitative perfusion analysis. METHOD:20 patients (mean age 63 years, 47-71) with biopsy proven prostate cancer underwent transrectal ultrasound (TRUS) prior to radical prostatectomy by 2 experien...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2012-1594
更新日期:2012-01-01 00:00:00
abstract::There are few studies on haemorheological disturbances in morbidly obese patients. The role played by the metabolic syndrome on the rheological profile of morbidly obese subjects has not yet been established, and it is not clear whether morbidly obese, but "metabolically healthy", show rheological alterations. We aime...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2011-1417
更新日期:2011-01-01 00:00:00
abstract::We investigated relationships among body composition, blood rheology, and exercise performance in 14 rugbymen (19-31 yr, weight 65.8-109.2 kg, height 1.7-1.96 m, body mass index 21.7-33.1 kg/m2) who underwent a standardized submaximal exercise session on cycloergometer corresponding to 225 kJ over 30 min. The rheologi...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::Despite increasing advances in microvascular free tissue transfer, flap failures, most commonly resulting from thrombosis at the anastomotic vascular site, remain a significant concern. Although several experimental and clinical studies have been carried out, no consensus has been reached so far on the efficacy, dosag...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章,评审
doi:10.3233/CH-2009-1220
更新日期:2009-01-01 00:00:00
abstract::In this study we determine the effects of reducing blood glucose on mean arterial blood pressure (MAP) and hematocrit (Hct) in patients with type 2 diabetes who are not responding to conventional treatment in an intensive treatment program 1 year after initiation of treatment. Data on MAP, glucose and Hct was obtained...
journal_title:Clinical hemorheology and microcirculation
pub_type: 临床试验,杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::Growth hormone deficiency (GHD) in adults results in alterations of body composition and metabolism associated with a lowered insulin sensitivity and an increased cardiovascular risk. Since hemorheologic disturbances (putative factors of vascular risk) are found in the insulin-resistance syndrome, we investigated bloo...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::In the present study, the role of the nucleus and its contribution to the deformability of the passive neutrophils was investigated. To determine the rheological properties of the nucleus and of the neutrophil itself, deformation tests on single neutrophil and sequestered nucleus have been performed by micropipette un...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2010-1326
更新日期:2010-01-01 00:00:00
abstract:PURPOSE:To compare the value of high-resolution contrast-enhanced ultrasound in the detection and characterisation of endoleaks after EVAR. MATERIAL AND METHODS:In this retrospective study, a cohort of 80 patients underwent both standard examination using a curved array 5 MHz transducer and a second examination using ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-141896
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Hemodialysis patients have increased mortality from cardiovascular complications. Whole blood viscosity (WBV) and red cell aggregation (RCA) may influence the pathogenesis of vascular complications in this population. The objective of this study was to determine whether the hemodialysis treatment or vascular...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2012-1532
更新日期:2012-01-01 00:00:00
abstract::In order to further characterize the alterations of erythrocyte aggregation described in ovarian cancer, we measured it with laser backscattering in eleven women suffering from ovarian cancer (mean age: 44.7 +/- 3.6, extreme values: 28-61 yr) compared with thirteen matched control women. Blood rheology exhibited a wid...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND:The optimal timing of remote ischemic preconditioning (RIPC) in renal ischemia-reperfusion (I/R) injury is still unclear. We aimed to compare early- and delayed-effect RIPC with hematological, microcirculatory and histomorphological parameters. METHODS:In anesthetized male CrI:WI Control rats (n = 7) laparo...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-200916
更新日期:2020-01-01 00:00:00
abstract::The objective of our research was to reveal the effects of different shear stresses on the expression of proto-oncogenes c-fos and c-myc in cultured human umbilical vein endothelial cells (HUVEC). A parallel plate flow chamber was used to control the value and duration of shear stress (SS), and the expression of c-fos...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Biomaterials research is expected to forward new materials to be used as, e.g., implant materials or as scaffolds for tissue engineering. It is central for such a scaffold material to create the track on which those cells can inhabit the scaffold needed to rebuild functional tissue substitutes. The tissue engineering ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Erythrocytes kept outside the blood circulation undergo progressive changes in metabolism, shape and function, which was the topic of this study. For that purpose, blood anticoagulated with either heparin, citrate or EDTA was incubated at temperatures of 5°C, 22°C or 37°C for 0 h, 24 h and 48 h, respectively. A temper...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-131682
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Purpura fulminans (PF) is a distinct form of rare meningococcal septicaemia mostly in childhood which is characterized by high lethality, extensive necroses and mutilations of extremities. Other ethiologies are idiopathic forms or purpura neonatorum, which is marked by deficiency of Protein-C. PF is caused b...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-190588
更新日期:2020-01-01 00:00:00
abstract::The present experiment attempted to evaluate the effect of electrical acupuncture on the cerebral microcirculation in anesthetized rats, using fluorescence videomicroscopy. Changes in the pial arteriolar diameter under acute hemorrhagic hypotension were examined quantitatively. The present results suggest that acupunc...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Raloxifene, the prototype of the selective estrogen receptor modulators, has been associated with an increased risk of venous thromboembolism. As hemorheological factors may be involved in thrombus formation this placebo-controlled study investigated whether raloxifene was associated with changes in determinants of bl...
journal_title:Clinical hemorheology and microcirculation
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2002-01-01 00:00:00
abstract:OBJECTIVE:The aim of our study was to evaluate the role of preoperative US, CEUS, and 99mTc-MIBI scanning with SPECT/CT in localizing diseased parathyroid glands in cases of refractory secondary hyperparathyroidism (SHPT). MATERIAL AND METHODS:Using pathological results as the gold standard, we compared the operative ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-190723
更新日期:2020-01-01 00:00:00
abstract:INTRODUCTION:Laparoscopy is more beneficial than the conventional open technique, however the pneumoperitoneum created may have an ischemic side effect. OBJECTIVE:Our aim was to evaluate the protective effects of preconditioning during laparoscopic cholecystectomies (LC). METHODS:30 patients were randomized into 2 gr...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-170336
更新日期:2018-01-01 00:00:00