Abstract：
:The objective of our research was to reveal the effects of different shear stresses on the expression of proto-oncogenes c-fos and c-myc in cultured human umbilical vein endothelial cells (HUVEC). A parallel plate flow chamber was used to control the value and duration of shear stress (SS), and the expression of c-fos and c-myc protein was measured by immunocytochemistry methods and image analysis software. Some important conclusions were drawn. In the stationary state, c-fos protein levels were very low. The SS's of 4 dyn/cm(2) and 10 dyn/cm(2) induced rapid increases of c-fos protein levels, especially the SS of 10 dyn/cm(2). The levels peaked at 1.0 h. Then, c-fos protein levels began to decrease, after 2.5 h, they declined to almost basal levels. In the stationary state, the c-myc protein levels were also very low slowly increasing after the onset of shear stress. The effects of 4 dyn/cm(2) and 10 dyn/cm(2) SS on c-myc protein expression levels had no difference and apparently were less than the effect on c-fos protein levels. The c-myc protein levels peaked at 1.5 h. Subsequently, they fell to basal levels at 2.5 h. Increased expression of these proto-oncogenes mediated by shear stress may have important effects on the regulation of critical cell activities, such as proliferation and differentiation.
journal_name
Clin Hemorheol Microcircjournal_title
Clinical hemorheology and microcirculationauthors
Li C,Zeng Y,Hu J,Yu Hsubject
Has Abstractpub_date
2002-01-01 00:00:00pages
117-23issue
2eissn
1386-0291issn
1875-8622journal_volume
26pub_type
杂志文章abstract：:Vascular endothelial cells form a monocellular layer on blood vessel walls with an estimated mass of 1.5 kg. One of the roles of endothelial cells is to control the hemodynamics through various metabolic activities affecting homeostasis, vascular tonus, blood fluidity, coagulating properties and blood cell adhesion. I...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:Radiographic contrast media can lead to drastic changes of the morphology of erythrocytes. The change of the erythrocyte morphology is associated with a decreased deformability possibly resulting from distinctions in the loss of constituents of the membrane cytoskeleton. However, it is unclear whether there is an intr...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-141879
更新日期：2014-01-01 00:00:00
abstract：OBJECTIVE:To assess sex-related differences in forearm skin microvascular reactivity to vascular occlusion and cardiovascular health in young healthy women and men. METHODS:203 young healthy individuals (67 men/136 women) participated in this study. Post-occlusive reactive hyperemia (PORH) in skin microcirculation fol...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-180483
更新日期：2019-01-01 00:00:00
abstract：:The aim of this work was to perform a comparative investigation of erythrocyte aggregability changes in the peripheral and cerebral circulation during ischemic and hemorrhagic stroke. Subjects of the present study were patients with ischemic brain infarcts (14 patients) and with hemorrhagic stroke (21 patients) from t...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：BACKGROUND:It is difficult to assess tumors of the parotid gland preoperatively. However, it is essential for the surgeon to know which kind of tumor is present. Ultrasound is the method of choice, but there is still no reliable differential diagnostic tool for determining whether a tumor is malignant or benign. OBJEC...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-179223
更新日期：2017-01-01 00:00:00
abstract：OBJECTIVE:This study aims to investigate the effect of nimodipine combined with betahistine on the levels of CRP and other inflammatory cytokines, as well as vascular endothelial function in patients with hypertensive cerebral vasospasm. METHODS:A total of 80 patients with hypertensive cerebral vasospasm from March 20...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-190589
更新日期：2020-01-01 00:00:00
abstract：:In the vascular system, the shear applied to the vascular wall activates mechano-sensors located on endothelial cells (ECs) leading to a modification in the gene expression profile. We applied laminar shear stress at 1 Pa on ECs for 6 h and measured by quantitative real time PCR the expression modulation of genes impl...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：OBJECTIVE:To investigate the extent to which B-flow and B-flow with 3D postprocessing and speckle reduction imaging (SRI) have advantages in appraising the morphology of a high-grade stenosis of the internal carotid artery (ICA) for preinterventional planning and for postinterventional ultrasonographic follow-up. MATE...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:Ischemic recruitment of endothelial progenitor cells (EPCs) in involved in compensatory angiogenic in animal models, but this still needs to be substantiated in humans. We enrolled 12 patients, who underwent surgical correction of abdominal aortic aneurysm without atherosclerosis of leg arteries (n = 4) or lower limb ...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-2011-1437
更新日期：2012-01-01 00:00:00
abstract：:Shear stress is known to induce platelet activation and aggregation. The red blood cell (RBC) aggregation test requires the application of shear stress for the cells to disaggregate for initialization. We tested the hypothesis that applying shear stress may activate platelets, which can influence RBC aggregation. The ...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-151969
更新日期：2016-01-01 00:00:00
abstract：:The neurologic outcome of 23 seven-year old children who had cord blood hyperviscosity was compared with that of children with normal cord blood viscosity in a randomised, controlled and blinded study. Viscosity was measured using a coaxial narrow-gap couette viscometer. Sixteen (69.6%) of the children with hypervisco...
journal_title：Clinical hemorheology and microcirculation
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1997-01-01 00:00:00
abstract：:The processing of polymers for blood contacting devices can have a major influence on surface properties. In this study, we fabricated poly(ether imide) (PEI) membranes and films to investigate the effects of the processing on physicochemical surface properties by atomic force microscopy (AFM), scanning electron micro...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-2010-1351
更新日期：2010-01-01 00:00:00
abstract：BACKGROUND:Red blood cells (RBC) are exposed to varying shear stress while traversing the circulatory system; this shear initiates RBC-derived nitric oxide (NO) production. OBJECTIVE:The current study investigated the effect of varying shear stress dose on RBC-derived NO production. METHODS:Separated RBC were prepare...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-189412
更新日期：2019-01-01 00:00:00
abstract：:Accumulated uremic toxins like indoxyl sulphate, hippuric acid and p-cresyl sulphates in renal failure patients stimulate proinflammatory effects, and consequently kidney and cardiovascular diseases. Low clearance rate of these uremic toxins from the blood of uremic patients by conventional techniques like hemodialysi...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-168046
更新日期：2016-01-01 00:00:00
abstract：UNLABELLED:Revascularization after long term aortic ischaemia in vascular surgery induces reperfusion injury accompanied with oxidative stress and inflammatory responses. The hypothesis of this study was that the aortic occlusion followed by controlled reperfusion (CR) can reduce the ischaemia-reperfusion injury, the s...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-152038
更新日期：2016-09-12 00:00:00
abstract：:In this study we determine the effects of reducing blood glucose on mean arterial blood pressure (MAP) and hematocrit (Hct) in patients with type 2 diabetes who are not responding to conventional treatment in an intensive treatment program 1 year after initiation of treatment. Data on MAP, glucose and Hct was obtained...
journal_title：Clinical hemorheology and microcirculation
pub_type： 临床试验,杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Hypercoagulable states due either to inherited or acquired thrombotic risk factors are only present in approximately half of cases of DVT, but the causes in the other half, remain unknown. The importance of biological risk factors such as hyperlipidemia, hypofibrinolysis and hemorheological alterations in the pathogen...
journal_title：Clinical hemorheology and microcirculation
pub_type：
doi：
更新日期：2002-01-01 00:00:00
abstract：:We studied the effect of mechanical forces (shear stress) on the kinetics of internalization of native LDL and ox-LDL in endothelial cell line ECV304. This study was performed by using Confocal microscopy and FRET with two carbocyanine dyes, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiO) as t...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:Cell-deformability characterization involves general measurement of highly complex relationships between cell biology and physical forces to which the cell is subjected. The review takes account of the modern technical solutions simulating the action of the force applied to the red blood cell in macro- and microcircul...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章,评审
doi：10.3233/CH-2009-1187
更新日期：2009-01-01 00:00:00
abstract：:The present investigation was designed to evaluate the acute effect of aerobic exercise on oxidative stress and the flow properties of the blood. Fourteen clinically healthy subjects (7 men and 7 women aged 56+/-19 yr) underwent maximal treadmill exercise with blood samples drawn prior to and immediately after exercis...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：OBJECTIVES:To identify the associated factors for malignancy in partially cystic thyroid nodules (PCTNs) on ultrasound (US). MATERIALS AND METHODS:This is a retrospective study. 338 PCTNs confirmed by surgery was included in this study. The US features of the nodules were reviewed and their significance in differentia...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-190582
更新日期：2020-01-01 00:00:00
abstract：:Beta thalassemia major is an inherited impairment of haemoglobin structure, in which there is partial or complete failure to synthesize a specific type of globin chain. The study was undertaken to assess the hemorheological changes in beta thalassemic major patients. We studied hemorheological parameters in thalassemi...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:Impaired endothelial responsiveness to specific vasodilator stimuli has been used as a surrogate marker of cardiovascular risk. Multiple methods allow testing endothelial responses in both microvessels and conduit arteries, but it is still unclear whether there is a relationship in endothelial function between these t...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：OBJECTIVE:To investigate macro- and microperfusion during 14 days of treatment with a new 2-layer compression system (3M™ Coban™ 2 Lite), designed for patients with leg ulcer and concomitant peripheral arterial occlusive disease. METHODS:A single-centre, open-label, prospective pilot study was performed with 15 subjec...
journal_title：Clinical hemorheology and microcirculation
pub_type： 临床试验,杂志文章
doi：10.3233/CH-2012-1568
更新日期：2013-01-01 00:00:00
abstract：:Development of new drugs is a sophisticated process that requires several, different methods. In our experiments we have applied two rheological models to study experimental and clinically used drugs. The antioxidant properties of several agents were estimated by erythrocyte filtration technique. The known antioxidant...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:The effects of various aldehydes such as secondary products of peroxidized lipids and other aldehydes on rheological parameters and their relation with aging process were studied. Malondialdehyde (MDA) in different concentrations can increase significantly viscosity and plastic viscosity of erythrocytes suspended in p...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：PURPOSE:Real-time shear-wave elastography (SWE) is a newly developed technique for the sonographic quantification of tissue elasticity, which already is used in the assessment of breast and thyroid lesions. Due to limited overlying tissue, the testes are ideally suited for assessment using shear wave elastography. To o...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-162046
更新日期：2016-01-01 00:00:00
abstract：:Asian tradition medicine (ATM), such as herbal medicine and acupuncture, has been widely used in the treatment of diseases, but the effects still remain unclear at the level of the microcirculation. Up to now, a variety of approaches have been made to comprehensively evaluate the significance of ATM. Microcirculatory ...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：BACKGROUND:Patients having coronary artery disease treated by coronary bypass or PCI procedure are exposed to tissue damage because of the phenomenon called reperfusion injury. Reperfusion injury can be characterized/monitored by oxidative stress parameters, inflammatory markers and by post-operative complication rate....
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章,随机对照试验
doi：10.3233/CH-170270
更新日期：2017-01-01 00:00:00
abstract：INTRODUCTION:The hormones erythropoietin (EPO) and thrombopoietin (TPO) are main regulators of erythro- and thrombopoiesis. Cell loss caused by operative procedures may alter serum levels of the hormones, resulting in well known phenomenons like reactice thrombocytosis. MATERIAL AND METHODS:Blood samples from 10 patie...
journal_title：Clinical hemorheology and microcirculation
pub_type： 杂志文章
doi：10.3233/CH-2011-1489
更新日期：2011-01-01 00:00:00
