Expression of Toll-like receptors in cultured nasal epithelial cells.

Abstract:

CONCLUSIONS:Nasal epithelial cells are constitutively equipped with all Toll-like receptors (TLRs) which are essential for innate immunity. Both mRNA and protein levels of TLR3 expression increased in more differentiated nasal epithelial cells. Considering that the ligand for TLR3 is viral dsRNA, this result is in good accordance with previous reports demonstrating that more differentiated airway epithelial cells have increased resistance to rhinovirus infection. OBJECTIVE:Nasal epithelial cells use innate immune responses to combat inspired potential pathogens. TLRs are receptors that recognize pathogen-associated molecular patterns of microbes. Therefore, we investigated the expression of TLRs in cultured nasal epithelial cells obtained from nasal polyps. MATERIALS AND METHODS:Submerged single layer (SSL) and air-liquid interface (ALI) nasal epithelial cell cultures with or without 10(-7) M retinoid acid (+/- RA) were created. RESULTS:ALI + RA culture developed ciliary differentiation as observed by light and scanning electron microscopic examination in 3 weeks. It had higher interleukin (IL)-8 basal secretion (21.9 vs 0.82-1.45 ng/ml) and transepithelial potential (-20.4 mV). TLR1-10 mRNA expression in cultured nasal epithelial cells was determined by RT-PCR. Only TLR3 mRNA significantly increased at day 20 vs day 1 (n=5, p=0.02) in ALI + RA cell culture. Higher TLR3 protein was also expressed at day 20 in ALI + RA cell culture but not in SSL culture by western blotting.

journal_name

Acta Otolaryngol

journal_title

Acta oto-laryngologica

authors

Lin CF,Tsai CH,Cheng CH,Chen YS,Tournier F,Yeh TH

doi

10.1080/00016480601089416

subject

Has Abstract

pub_date

2007-04-01 00:00:00

pages

395-402

issue

4

eissn

0001-6489

issn

1651-2251

pii

777066536

journal_volume

127

pub_type

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