Abstract:
OBJECTIVE:Proteomic analysis with 2D electrophoresis and matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) may be a powerful tool for identifying and characterizing the specific proteins relating to the pathogenesis of some diseases, including cholesteatoma. The purpose of this study was to identify upregulated proteins in human cholesteatoma in comparison with canal skin using proteomic analysis. MATERIAL AND METHODS:Three cholesteatoma matrices and three samples of normal retroauricular skin were obtained intraoperatively from cholesteatoma patients. We performed 2D electrophoresis in order to separate the proteins by molecular weight and approximately detected 600 protein spots. We then analyzed the 17 upregulated spots from the cholesteatoma matrices using MALDI-TOF MS. Upregulation of proliferating cell nuclear antigen (PCNA) and osteoclast stimulating factor-1 (OSF-1), two candidate proteins in the pathogenesis of cholesteatoma, was confirmed by means of immunohistochemistry and reverse transcriptase polymerase chain reaction. RESULTS:Interestingly, two candidate proteins, PCNA and OSF-1, relating to cellular proliferation and bone destruction were identified in the cholesteatoma matrices and we also detected nine proteins relating to the mechanism of signal transduction in the pathogenesis of cholesteatoma, including P-13-kinase P55 gamma subunit, RET proto-oncogene tyrosine kinase receptor and adenosine kinase. CONCLUSION:Proteomic analysis may be a powerful tool for the identification and characterization of many promising candidate proteins relating to cholesteatoma.
journal_name
Acta Otolaryngoljournal_title
Acta oto-laryngologicaauthors
Kim JL,Jung HHdoi
10.1080/00016480410017882subject
Has Abstractpub_date
2004-09-01 00:00:00pages
783-8issue
7eissn
0001-6489issn
1651-2251pii
Q7P6AKNXQ7D4VG1Wjournal_volume
124pub_type
杂志文章abstract::The occurrence of symptoms from the upper airways in the furniture industry was studied by means of a questionnaire which was answered by 676 wood furniture workers from 50 different factories. Twenty per cent reported nasal hypersecretion and 40% nasal obstruction. A randomized sample of 61 workers was investigated f...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488409107596
更新日期:1984-11-01 00:00:00
abstract::The influence of posture on nasal cavity geometry was studied by acoustic rhinometry in 14 normal adult subjects. On adopting a supine position from sitting the volume of the initially more patulous nasal cavity decreased and the volume of the less patulous side increased. These changes in volume are statistically sig...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489309135858
更新日期:1993-07-01 00:00:00
abstract:CONCLUSION:Results from a database for sudden sensorineural hearing loss (SSNHL) demonstrate no correlation between laboratory findings, treatment, and outcome in 400 patients. The patients with pathological test results were not treated differently from those with normal test results. The value of laboratory findings ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480903161541
更新日期:2010-03-01 00:00:00
abstract:CONCLUSION:The narrowing pattern of the upper airway in obstructive sleep apnea patients may be different in sleep as compared with awake. Three different types of obstruction were observed in these subjects during drug-induced sleep. The different obstruction pattern during drug-induced sleep suggests that different s...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016480902780242
更新日期:2009-12-01 00:00:00
abstract:CONCLUSIONS:We describe a Japanese family with high-frequency sensorineural hearing loss (SNHL) harboring a c.211delC mutation in the KCNQ4 gene. Families showing progressive high-frequency SNHL should be investigated for mutations in the KCNQ4 gene. OBJECTIVE:To determine the responsible deafness gene in a Japanese f...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2014.890740
更新日期:2014-06-01 00:00:00
abstract::The transitional zone of the saccule and the utricle--has been studied by transmission electron microscope in rats, days before birth. A noticeable secretory activity has been observed in their cells as well as the presence of inorganic spicular bodies that have also been observed in the lumen, some of them integrated...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488309139436
更新日期:1983-05-01 00:00:00
abstract:CONCLUSIONS:The presented results add further support to the observation that laser microsurgery is the preferential surgical treatment for recurrent respiratory papillomatosis (RRP). A meticulous follow-up for early recognition of local recurrence and malignant transformation is recommended. OBJECTIVES:Endoscopic mic...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480701200350
更新日期:2007-11-01 00:00:00
abstract::To elucidate the effects of CO2 in the middle ear upon the cochlea, measurements were made of the cochlear potentials (compound action potential and endocochlear potential) and of the pH of the inner ear fluids and the organ of Corti. Gas containing CO2 did not affect the AP threshold, except for a slight decrease in ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488909107397
更新日期:1989-07-01 00:00:00
abstract::Background: It is beneficial for CI patients listen to music. However it is necessary to take steps to improve the musicality of CI patients.Objectives: The aims of the study were to evaluate the primary musicality of children with cochlear implants versus those with normal hearing.Material and methods: Children parti...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2020.1766699
更新日期:2020-09-01 00:00:00
abstract::The submucosal glands in the rat nose are characterized by the presence of both neutral and acid glycoproteins, which are important constituents of nasal and tracheobronchial secretions. This study is an attempt to gain a better insight into the secretion of these two types of glycoproteins and its neural regulation. ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488709128287
更新日期:1987-11-01 00:00:00
abstract::Seven to eight years after a disease period of vestibular neuritis 19 patients previously examined at our department were given a questionnaire about whether they had experienced any audiovestibular symptoms since they first fell ill. All 19 patients responded and 18 consented to participate in the long-term follow-up...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489950180243
更新日期:1999-01-01 00:00:00
abstract::Normal quantum type bone remodeling was studied in temporal bones of young domestic pigs by means of combined microradiography, osteofluorochrome labelling and osteoid staining. Evolving secondary osteons were morphologically normal although much less in the otic capsule than in the surrounding compacta of the tempora...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489009122540
更新日期:1990-09-01 00:00:00
abstract::Background: Chronic exposure to whole body vibration can affect the gastrointestinal system, the lumbar spine, the peripheral veins and the vestibulocochlear system. Aims/Objectives: The aim of this study was to evaluate the effects of vibration on the vestibular system in noisy and noise-free environments using the v...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2019.1666213
更新日期:2019-11-01 00:00:00
abstract::It has previously been shown that simple compounds with multiple amine groups are ototoxic, the degree of ototoxicity depending on the number of amine groups in the molecule. The relationship between the number of amino groups and ototoxicity in aminoglycoside was studied using kanamycin A and kanamycin B, which conta...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488709134700
更新日期:1987-01-01 00:00:00
abstract:CONCLUSION:Selected patients with Meniere's disease (MD) show an improvement of disability from vertigo after transtympanic ventilation tube insertion, although an effect on vestibular function is not seen if ipsilateral middle ear pressure lower than -50 daPa is used as a selection criterion. OBJECTIVES:Transtympanic...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016480902791678
更新日期:2009-12-01 00:00:00
abstract:CONCLUSION:Concomitant antiemetic therapy comprising fosaprepitant, palonosetron, and dexamethasone is effective for head and neck carcinoma. OBJECTIVE:A patient diary was constructed to determine the effectiveness of concomitant antiemetic therapy with a neurokinin-1 receptor antagonist (fosaprepitant), 5-hydroxytryp...
journal_title:Acta oto-laryngologica
pub_type: 临床试验,杂志文章
doi:10.3109/00016489.2014.913314
更新日期:2014-11-01 00:00:00
abstract::A case-control design was used to assess eye-head-trunk coordination for community-dwelling elders performing a stand-walk task. Eighteen elders with a high risk of falling were matched to 18 subjects with low risk on the basis of age, living status (living alone or with someone) and category of residence (independent...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480152583656
更新日期:2001-09-01 00:00:00
abstract:CONCLUSIONS:Flat-panel cone-beam computed tomography (CBCT) is able to assess the trajectory of the implanted cochlear implant (CI) array. This is essential to determine specific effects of electrode design and surgical innovations on outcomes in cochlear implantation. CBCT is a non-invasive approach yielding similar d...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2011.644805
更新日期:2012-04-01 00:00:00
abstract:CONCLUSION:Although sentinel lymph node (SLN) biopsy is not yet validated for clinical use to replace elective neck dissection in patients with oral squamous cell carcinoma, it can be recommended for patients who do not fulfil the criteria for elective neck treatment according to current treatment protocols. OBJECTIVE...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480701362002
更新日期:2008-01-01 00:00:00
abstract::Background: The various pathological types of non-squamous cell carcinomas (nSCCs) of the larynx accounted for about 5% laryngeal malignancies, but the knowledge regarding these pathological behaviors, therapeutic models and prognostic factors was limited.Objectives: To investigate the clinical characteristics and the...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2019.1700305
更新日期:2020-02-01 00:00:00
abstract::Gaze functions under high-frequency head oscillations with large amplitudes were investigated in 12 normal subjects. Oscillation amplitude decreased as the frequency increased (31 degrees at 2 Hz to 11 degrees at 5 Hz on average). Maximum head velocity and acceleration were 170-200 degrees/s and 2,500-5,000 degrees/s2...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488909127495
更新日期:1989-03-01 00:00:00
abstract::In a study on the effects of a frequency-dependent automatic gain control in hearing-aids, two experiments were carried out with hearing-impaired listeners. In the first experiment, the effect of varying the amplitude-frequency response on the speech-reception threshold (SRT) of sentences presented in noise was studie...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.1990.12088430
更新日期:1990-01-01 00:00:00
abstract::Dynamic range (DR) of cochlear implant electrodes and speech recognition scores (SRS) were chronologically studied in 23 post-lingually deaf subjects over a period of one year. DR significantly increased over one year (ANOVA, p = 0.03). When DR was compared among three groups of electrodes which were located in the in...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489609137829
更新日期:1996-03-01 00:00:00
abstract:CONCLUSIONS:Klotho protein content decreases with increasing age, which weakens resistance to oxidative stress, resulting in induced cell death as well as modulating endolymph fluid homeostasis. Down-regulation of klotho also leads to down-regulation of TRPV5 and TRPV6, resulting in modified Ca(2+) homeostasis in the i...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016480902725254
更新日期:2009-12-01 00:00:00
abstract::Preyer's reflex, the elicitation of startle response to auditory stimuli, has been widely used for the evaluation of hearing in rodents and other animals. Surprisingly, however, the sensitivity and specificity of Preyer's reflex in the assessment of hearing has not been adequately studied. The aim of this study was to...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:
更新日期:2001-07-01 00:00:00
abstract:CONCLUSIONS:In skilled hands, selective embolization is a safe procedure and represents an effective treatment for prolonged epistaxis. Embolization therapy can be repeated if necessary. OBJECTIVE:Severe posterior epistaxis is a common clinical problem in an ENT department and controlling the bleeding may present diff...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480410023029
更新日期:2005-03-01 00:00:00
abstract::To investigate whether the crossed olivo-cochlear bundle (COCB) functions in a protective manner, albino guinea pigs were exposed to sounds of varying intensity (110-130 dB SPL, 3-30 min) with or without electric stimulation of COCB, and the threshold shifts of the compound action potential (CAP) were examined. A stat...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.1992.11665405
更新日期:1992-01-01 00:00:00
abstract:CONCLUSION:This study suggests that hyperbilirubinemia in the neonatal rat can impair auditory function and induce peripheral nerve pathology by reducing neurofilament-positive cells in spiral ganglion neurons (SGNs). This finding indicates a potential connection between hyperbilirubinemia and auditory impairment. OBJ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2014.938361
更新日期:2014-11-01 00:00:00
abstract::The early development of human otoconial membranes was studied by scanning electron microscopy and X-ray microanalysis; 87 specimens, ranging from 7 to 12 weeks gestational age were examined. A few otoconia, which contained calcium, were present in the utricle by the end of the seventh week. By 8 weeks, both the saccu...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488209128884
更新日期:1982-07-01 00:00:00
abstract::ESS is a form of minimal access surgery that includes different tasks and manoeuvres requiring sophisticated psychomotor coordination with varying levels of force application. The avoidance of complications is partially dependent upon reducing surgical force application when operating against vital barriers such as th...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480410016568
更新日期:2004-03-01 00:00:00