Abstract:
:Monoclonal antibody (mAb) MN423 recognizes Alzheimer's disease specific conformation of tau protein assembled into paired helical filaments (PHF). Since the three-dimensional structure of PHF is currently unavailable, the structure of MN423 binding site could provide important information about PHF conformation with the consequences for the Alzheimer's disease prevention and cure. Fab fragment of MN423 was prepared and purified. We have identified two different conditions for crystallization of the Fab fragment that yielded two crystal forms. They diffracted to 3.0 and 1.6 A resolution with four and one molecule in the asymmetric unit, respectively. Both crystal forms belonged to the space group P2(1) with unit cell parameters a = 76.4 A, b = 138.4 A, c = 92.4 A, beta = 101.9 degrees , and a = 71.5 A, b = 36.8 A, c = 85.5 A, beta = 113.9 degrees .
journal_name
Protein Pept Lettjournal_title
Protein and peptide lettersauthors
Csóková N,Skrabana R,Urbániková L,Kovácech B,Popov A,Sevcík J,Novák Mdoi
10.2174/092986606778256180subject
Has Abstractpub_date
2006-01-01 00:00:00pages
941-4issue
9eissn
0929-8665issn
1875-5305journal_volume
13pub_type
杂志文章abstract::Background. Obesity is a major health problem in both developed and emerging countries. Obesity is a complex disease whose etiology involves genetic factors in strong interplay with environmental determinants and lifestyle. The discovery of genetic factors and biological pathways underlying human obesity is hampered b...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/09298665113209990053
更新日期:2014-01-01 00:00:00
abstract::Interaction between S100P and its target protein is an essential step in several cellular functions. The amphipathic mellitin peptide binds tightly to S100P protein in the presence of calcium cation. Since little is known about the recognition sequence, mellitin interaction form a model for S100P. Interaction between ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606775338380
更新日期:2006-01-01 00:00:00
abstract::Glycation, a non-enzymatic reaction between glucose and protein is the primary cause of diabetic complications. Albumin, the most abundant plasma protein undergoes glycation both in vivo and in vitro. The influence of albumin on glycation of less abundant proteins has not been addressed. For the first time, we show th...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608785133690
更新日期:2008-01-01 00:00:00
abstract::Here, we show for the first time that non-fibrillar and spherical aggregates produced from PrP-(23-98) in the presence of NADPH plus copper ions are toxic to cultured cells and induce apoptotic signals. It is also confirmed that endogenous cellular PrP isoform is not required for toxicity to occur. ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609787049475
更新日期:2009-01-01 00:00:00
abstract::Phage Virion Proteins (PVP) are essential materials of bacteriophage, which participate in a series of biological processes. Accurate identification of phage virion proteins is helpful to understand the mechanism of interaction between the phage and its host bacteria. Since experimental method is labor intensive and t...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866526666190410124642
更新日期:2020-01-01 00:00:00
abstract::Most proteinaceous pores are characterized as ionic channels. However, some are also involved in protein translocation through phospholipidic membranes. This concept has evolved slowly in cell biology and in biophysics, requiring the development of adapted electrical and biochemical methods. Protein translocation in m...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/09298665113209990076
更新日期:2014-03-01 00:00:00
abstract::The mutation, conferring streptomycin and deoxyglucose resistance on cells, had profound effect on the kinetic and thermodynamic parameters inferring thermostabilization of beta-glucosidase from mutant 51 SM(r) of Cellulomonas biazotea. Free energy of activation for substrate binding, enthalpy and entropy of activatio...
journal_title:Protein and peptide letters
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更新日期:2005-04-01 00:00:00
abstract::Hepatitis C virus and related viruses in the Flaviviridae family (such as dengue virus, yellow fever virus or West Nile virus) are amongst the most important human pathogens, causing substantial morbidity and mortality world-wide. The production of viral progeny in Flaviviridae is orchestrated by the small, multifunct...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
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更新日期:2010-08-01 00:00:00
abstract::Colon cancer is one of the most common malignances. In vitro and in vivo study show that retinoic acids inhibit a wide variety of cancer cells but the molecular mechanism of their anti-tumor effects are not yet fully understood. Alltrans retinoic acid (ATRA), an isomer of retinoic acid, can inhibit the proliferation o...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612803521675
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND:Unstructured regions in proteins can vary from several amino acid residues to a completely disordered sequence. Since such regions play an important role in the protein functioning, much attention is being paid to their prediction. Special different programs are available for this purpose; however, predictio...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866525666180621143957
更新日期:2018-01-01 00:00:00
abstract::From the extensive analysis, we identified three highly conserved sequence segments in HCV NS5A proteins and one binding motif in human proteins. The binding motif of human proteins often forms a full helix or an extended strand-loop structure, and is in good agreement with the experimental findings of previous studie...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608784567500
更新日期:2008-01-01 00:00:00
abstract::Cyclophilin A (CyPA) is a cytosolic receptor of immunosuppressive drug cyclosporin A (CsA) which possesses peptidyl-prodyl cis/trans isomerase (PPIase) activity. The recombinant human CyPA (rhCyPA) gene has been expressed in E. coli M15. Purification was performed using salting-out, as well as Sephacryl S-100 and DEAE...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611797642670
更新日期:2011-12-01 00:00:00
abstract::The immunogenicity of "novel" MART-1 and Tyrosinase class-II peptides was assessed in transgenic mice. Tyrosinase(141-161) peptide was found to be immunogenic and endogenously processed in the HLA-DRbeta1*0101 and HLA-DRbeta1*0401 transgenic mice with peptide specific production of IFNgamma or IL-5 respectively. The M...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986607780782768
更新日期:2007-01-01 00:00:00
abstract::G-protein betagamma dimers are prime regulators transmitting extracellular signals to wide-ranging cellular effectors including phosphoinositide 3-kinase (PI3K) isoforms beta and gamma. Recombinant Gbetagamma purified from Sf9 cells via metal-affinity and anion exchange chromatography exhibited a wortmannin-insensitiv...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609789055340
更新日期:2009-01-01 00:00:00
abstract::PepX is a X-prolyl dipeptidyl aminopeptidase of S15 family that cleaves dipeptides from the N-terminus of polypeptides having a proline or alanine residue at the second position. Involved in bacterial nutrition and in peptide maturation, this serine exopeptidase, counterpart of the mammalian DDP-4, has been proposed t...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::Bacterial ADP-ribosyltransferases (BADPRTs) are extensively contributed to determine the strain-specific virulence state and pathogenesis in human hosts. Understanding molecular evolution and functional diversity of the BADPRTs is an important standpoint to describe the fundamental behind in the vaccine designing for ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866511320090012
更新日期:2013-09-01 00:00:00
abstract::After several studies on prediction of mutation, we examine the effect of three sampling strategies, the sampling based on years, the sampling based on number of mutations, and the sampling based on the unpredictable portion of amino-acid pairs, on the prediction performance in H5N1 hemagglutinins. The results show th...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608785133735
更新日期:2008-01-01 00:00:00
abstract::Apomyoglobin (apoMb) folds through at least two partially folded forms that are detected both as transient intermediates during folding/unfolding kinetics or as stable intermediates at equilibrium. Here, I summarize the results of recent kinetic studies, which combined with detailed characterizations of equilibrium fo...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866053587174
更新日期:2005-04-01 00:00:00
abstract:BACKGROUND:Laminin, a member of the Extracellular Matrix (ECM), is a glycoprotein that is used as a factor that affects cell adhesion, proliferation, survival, and differentiation. Of these, five globular domains (LG domains) of the alpha chain play an important role in influencing the cell by binding to the integrin. ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866526666190617091155
更新日期:2019-01-01 00:00:00
abstract::Protein phosphatase magnesium-dependent 1, delta (PPM1D) is a member of the PPM1 (formerly PP2C) protein phosphatase family, and is induced in response to DNA damage. The overexpression of PPM1D is thought to exert oncogenic effects through the inhibition of tumor suppressor proteins. PPM1D shows high selectivity for ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608785849236
更新日期:2008-01-01 00:00:00
abstract::After consideration of the tertiary structure of the pY+3 binding pocket in SH2 domains, isoleucine of Ac-pYEEIE was replaced with various unnatural aliphatic amino acids and the binding affinities for Lck, Src, and Fyn SH2 domains were measured. We determined the characteristics of the amino acid at the pY+3 position...
journal_title:Protein and peptide letters
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更新日期:2004-12-01 00:00:00
abstract::Prediction of thermophilic and mesophilic protein plays a crucial role in both biochemistry and bioengineering. In this study, a different mode of pseudo amino acid composition (PseAAC) was proposed to formulate the protein samples by integrating the amino acid composition, the physic chemical features, as well as the...
journal_title:Protein and peptide letters
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更新日期:2011-07-01 00:00:00
abstract:BACKGROUND:Microbe-Binding Peptides (MBPs) are currently being investigated to address the problem of antimicrobial resistance. Strategies enhancing their antimicrobial activity have been developed, including peptide dimerization. Here, we present an alternative approach based on peptide polymerization, yielding hapten...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866527666200427212940
更新日期:2020-01-01 00:00:00
abstract::Identifying protein complexes in protein-protein interaction (PPI) networks is a fundamental problem in computational biology. High-throughput experimental techniques have generated large, experimentally detected PPI datasets. These interactions represent a rich source of data that can be used to detect protein comple...
journal_title:Protein and peptide letters
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doi:10.2174/0929866521666140320111720
更新日期:2014-07-01 00:00:00
abstract::Calcineurin (CN) is a heterodimer of a catalytic subunit, calcineurin A (CNA), and a regulatory subunit (CNB). Here, we find that the mechanism by which CNB regulates CNA depends on the substrate involved. The regulation mechanism involving tau and its truncation segments is distinct from that involving RII peptide, a...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609787316243
更新日期:2009-01-01 00:00:00
abstract::Quantum mechanical fragment molecular orbital calculations have been performed for receptor binding of the hemagglutinin protein of the recently pandemic influenza 2009 H1N1, A/swine/Iowa/1930, and A/Puerto Rico/8/1934 viruses to α2-6 linked sialyloligosaccharides, as analogs of human receptors. The strongest receptor...
journal_title:Protein and peptide letters
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doi:10.2174/092986611794927893
更新日期:2011-05-01 00:00:00
abstract::Familial mediterranean fever (FMF) is a systemic disorder characterized by recurrent attacks of fever and polyserositis. In FMF, several pro-inflammatory cytokines have been found to be elevated during the attacks. In recent years, it is shown that some proteins originated from adipose tissue play important role in in...
journal_title:Protein and peptide letters
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doi:10.2174/092986610792231555
更新日期:2010-10-01 00:00:00
abstract::The cyclotides are a large family of plant proteins that have a cyclic backbone and a knotted arrangement of three conserved disulfide bonds. Despite the apparent complexity of their cystine knot motif it is possible to efficiently fold these proteins, as exemplified by oxidative folding studies on the prototypic cycl...
journal_title:Protein and peptide letters
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更新日期:2005-02-01 00:00:00
abstract:BACKGROUND:The heterologous production of antimicrobial peptides in bacterial models can produce insoluble proteins due to the lack of proper folding. Fusion proteins have been used to increase the expression and solubility of these types of proteins with varying degrees of success. OBJECTIVES:Here, we demonstrate the...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866527666200610133407
更新日期:2020-06-10 00:00:00
abstract::Free radicals induce protein modifications, often associated to many biological phenomena. This mini-review overviews the approaches we have used to elucidate the radical-induced damages on sulfur-containing enzymes, such as ribonuclease and lysozyme, and the possible post-translational mechanism of the damage to anot...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/092986607781483840
更新日期:2007-01-01 00:00:00