Abstract:
:Successful treatment of multiple cancer types requires early detection and identification of reliable biomarkers present in specific cancer tissues. To test the feasibility of identifying proteins from archival cancer tissues, we have developed a methodology, termed direct tissue proteomics (DTP), which can be used to identify proteins directly from formalin-fixed paraffin-embedded prostate cancer tissue samples. Using minute prostate biopsy sections, we demonstrate the identification of 428 prostate-expressed proteins using the shotgun method. Because the DTP method is not quantitative, we employed the absolute quantification method and demonstrate picogram level quantification of prostate-specific antigen. In depth bioinformatics analysis of these expressed proteins affords the categorization of metabolic pathways that may be important for distinct stages of prostate carcinogenesis. Furthermore, we validate Wnt-3 as an upregulated protein in cancerous prostate cells by immunohistochemistry. We propose that this general strategy provides a roadmap for successful identification of critical molecular targets of multiple cancer types.
journal_name
Oncogenejournal_title
Oncogeneauthors
Hwang SI,Thumar J,Lundgren DH,Rezaul K,Mayya V,Wu L,Eng J,Wright ME,Han DKdoi
10.1038/sj.onc.1209755subject
Has Abstractpub_date
2007-01-04 00:00:00pages
65-76issue
1eissn
0950-9232issn
1476-5594pii
1209755journal_volume
26pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::Inhibitor of growth (ING)4, member of a gene family encoding potential tumor suppressors, is implicated as a repressor of angiogenesis and tumor growth and suppresses loss of contact inhibition in vitro. Here, we report that ING4 undergoes alternative splicing. Expression analysis identified novel ING4 spliced variant...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210335
更新日期:2007-08-09 00:00:00
abstract::The E1a gene of group C adenoviruses is one of the most studied transforming genes of DNA tumor viruses. These transforming genes have been conventionally considered as dominant oncogenes since they transform cells in vitro and many of the resulting transformed cell lines induce tumors in experimental animals. It now ...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:
更新日期:1992-07-01 00:00:00
abstract::Interleukin-6 (IL-6) is a cytokine that regulates the proliferation of some tumor cells including multiple myeloma (MM). Ectopic expression of fibroblast growth factor receptor 3 (FGFR 3) associated with the chromosomal translocation, t(4;14)(p16.3;q32), is frequently found in MM, and therefore, has been implicated in...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208782
更新日期:2005-09-15 00:00:00
abstract::Cellular senescence functions as a tumor suppressor that protects against cancer progression. α-Catulin, an α-catenin-related protein, is reported to have tumorigenic potential because it regulates the nuclear factor-κB (NF-κB) pathway, but little is known about its clinical relevance and the mechanism through which i...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.637
更新日期:2011-06-09 00:00:00
abstract::Recent studies have revealed that Ras can associate physically with Raf. In the present study, we tested 34 mutants of Ha-Ras carrying substitution(s) in the region of residues 23-71 for their ability to associate with Raf-1. Mouse Ba/F3 cell lysates were incubated with each mutant Ras protein, in either the guanosine...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
abstract::The heterogeneous breast cancers can be classified into different subtypes according to their histopathological characteristics and molecular signatures. Foxa1 expression is linked with luminal breast cancer (LBC) with good prognosis, whereas Twist1 expression is associated with basal-like breast cancer (BLBC) with po...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.286
更新日期:2017-02-23 00:00:00
abstract::The product of the proto-oncogene fos is a nuclear phosphoprotein. We have investigated if nuclear location of Fos protein is mediated by a nuclear targeting sequence. We show that the cytoplasmic chicken pyruvate kinase protein translocates to the nucleus if fused to the 22 amino acid Fos basic region (amino acids 13...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-11-01 00:00:00
abstract::LIM-kinase 2 (LIMK2) belongs to the LIMK family of proteins, which comprises LIMK1 and LIMK2. Both proteins regulate actin polymerization through phosphorylation and inactivation of the actin depolymerizing factor cofilin. In this study, we show that the level of LIMK2 protein is increased in neuroblastoma, BE(2)-C ce...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2009.367
更新日期:2010-01-28 00:00:00
abstract::The c-Met receptor is a potential therapeutic target for non-small cell lung cancer (NSCLC). Signaling interactions between c-Met and the mutant epidermal growth factor receptor (EGFR) have been studied extensively, but signaling intermediates and biological consequences of lateral signaling to c-Met in EGFR wild-type...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.84
更新日期:2011-08-18 00:00:00
abstract::The adenomatous polyposis coli (APC) tumor suppressor gene is mutationally inactivated in both familial and sporadic forms of colorectal cancers. In addition, hypermethylation of CpG islands in the upstream portion of APC, a potential alternative mechanism of tumor suppressor gene inactivation, has been described in c...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203704
更新日期:2000-07-27 00:00:00
abstract::LASP-1 is a multidomain protein predominantly localized at focal contacts, where it regulates cytoskeleton dynamics and cell migration. However, in different tumor entities, a nuclear LASP-1 accumulation is observed, thought to have an important role in cancer progression. Until now, the molecular mechanisms that cont...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.216
更新日期:2013-04-18 00:00:00
abstract::Overexpression of the nuclear phosphoprotein p53 has been detected in many different transformed human cell lines and primary adult tumors. Elevated steady-state levels of p53 appear to be the result of an increase in the stability of the protein and, in adult cancers, high levels of the protein are associated with mu...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::Cancer therapy drugs, such as diamminedichloroplatinum (cisplatin), mitomycin C, etoposide and a number of other compounds, as well as energy-rich radiation, are known to act on cellular DNA. These agents are shown to induce nuclear accumulation of the so-called tumor-suppressor protein p53 in fibroblastoid cells, as ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::Cancer stem cell (CSC), the primary source of cancer-initiating population, is involved in cancer recurrence and drug-resistant phenotypes. This study demonstrates that the loss of DAB2IP, a novel Ras-GTPase activating protein frequently found in many cancer types, is associated with CSC properties. Mechanistically, D...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2014.215
更新日期:2015-05-21 00:00:00
abstract::Cbl proteins are ubiquitin protein ligases, which ubiquitinate activated tyrosine kinases and target them for degradation. Both c-Cbl and Cbl-b have an ubiquitin associated (UBA) domain at their C-terminal end. We observed that high molecular weight ubiquitinated proteins constitutively coimmunoprecipitated with trans...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207952
更新日期:2004-09-16 00:00:00
abstract::The human ETS2 gene is a member of the ets gene family (ETS1, ETS2, ERG, ELK1 and ELK2) with amino acid similarity to the v-ets oncogene of the avian leukemia virus, E26. The ETS2 gene is composed of 10 exons, nine of which contribute to the open reading frame encoding 469 amino acids. The ETS2 gene directs the synthe...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::Activation of the Akt/PKB protein kinase family triggers increases in cell size, metabolism and survival. Akt coordinately regulates these fundamental cellular processes through phosphorylation-dependent inactivation of tumor suppressors and activation of trophic signaling. Akt signaling stimulates transport and metab...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1209097
更新日期:2005-11-14 00:00:00
abstract::The RNA-binding protein (RBP) RNPC1 is a target of the p53 family and forms a feedback regulatory loop with the p53 family proteins. The murine double minute-2 (MDM2) oncogene, a key negative regulator of p53, has a critical role in a variety of fundamental cellular processes. MDM2 expression is found to be regulated ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.238
更新日期:2013-04-25 00:00:00
abstract::Microarray RNA gene expression profiling analysis has shown that Sox4 (Sry-related high mobility group (HMG) box 4) is one of the most upregulated genes in adenoid cystic carcinoma (ACC), relative to non-neoplastic tissue of origin. Here, we show that Sox4 protein is similarly upregulated in ACC by immunohistochemistr...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209566
更新日期:2006-09-14 00:00:00
abstract::Somatostatin receptors (sst1-5) are present in different types of tumors, where they inhibit key cellular processes such as proliferation and invasion. Although ssts are densely expressed in breast cancer, especially sst2, their role and therapeutic potential remain uncertain. Recently, we identified a new truncated s...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.389
更新日期:2012-04-19 00:00:00
abstract::The C-terminal src kinase (Csk) is responsible for the phosphorylation of the carboxy-terminal tyrosine of several tyrosine kinases of the Src family. This phosphorylation site has a negative regulatory function. Csk is unique among the members of the protein tyrosine kinase family because it lacks the conserved tyros...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1993-05-01 00:00:00
abstract::We have compared the DNase I hypersensitivity of the regulatory region of two estrogen-regulated genes, pS2 and cathepsin D in hormone-dependent and -independent breast carcinoma cell lines. This strategy allowed the identification of two important control regions, one in pS2 and the other in cathepsin D genes. In the...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202317
更新日期:1999-01-14 00:00:00
abstract::Familial adenomatous polyposis (FAP) is caused by germline mutations in the adenomatous polyposis coli (APC) gene. Two promoters, 1A and 1B, have been recognized in APC, and 1B is thought to have a minor role in the regulation of the gene. We have identified a novel deletion encompassing half of this promoter in the l...
journal_title:Oncogene
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1038/onc.2011.201
更新日期:2011-12-15 00:00:00
abstract::Melanoma is a deadly form of skin cancer that accounts for a disproportionally large proportion of cancer-related deaths in younger people. Compared with most other skin cancers, a feature of melanoma is its high metastatic capacity, although the mechanisms that confer this are not well understood. The Hippo pathway i...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-020-1362-9
更新日期:2020-07-01 00:00:00
abstract::The function of the class III histone deacetylase, Sir2, in promoting lifespan extension is well established in small model organisms. By analogy, SirT1, the mammalian orthologue of Sir2, is a candidate gene to slow down aging and forestall the onset of age-associated diseases. We have used SirT1-null mice to study th...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2009.147
更新日期:2009-08-13 00:00:00
abstract::BAG-1 is a Hsp70/Hsc70-binding protein that interacts with Bcl-2, Raf-1, steroid hormone receptors, Siah-1, and hepatocyte growth factor (HGF) receptors, implying multiple functions for the BAG-1 protein. Here, we provide evidence that gene transfer-mediated overexpression of BAG-1 markedly enhances the motility of hu...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202661
更新日期:1999-05-27 00:00:00
abstract::The carboxyl terminus of c-Myb contains a negative regulatory domain that is absent in the v-Myb oncoprotein, but conserved among all the known Myb proteins of animals. This domain inhibits transcriptional activation by c-Myb in animal cells, but not in budding yeast, suggesting that additional protein(s) present in a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204048
更新日期:2001-01-04 00:00:00
abstract::Ataxia-Telangiectasia (A-T) is an autosomal recessive human disease characterized by genetic instability, radiosensitivity, immunodeficiency and cancer predisposition, because of mutation in both alleles of the ATM (ataxia-telangiectasia mutated) gene. The role of Atm heterozygosity in cancer susceptibility is controv...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2008.280
更新日期:2008-11-20 00:00:00
abstract::Suppressor of cytokine signaling 1 (SOCS1) is considered as a tumor suppressor protein in hepatocellular carcinoma (HCC), but the underlying mechanisms remain unclear. Previously, we have shown that SOCS1-deficient hepatocytes displayed increased responsiveness to hepatocyte growth factor (HGF) due to enhanced signali...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2015.20
更新日期:2015-11-12 00:00:00
abstract::PML is a component of a multiprotein complex, termed nuclear bodies, and the PML protein was originally discovered in patients suffering from acute promyelocytic leukaemia (APL). APL is associated with a reciprocal chromosomal translocation of chromosomes 15 and 17, which results in a fusion protein comprising PML and...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1204765
更新日期:2001-10-29 00:00:00