Abstract:
:BAG-1 is a Hsp70/Hsc70-binding protein that interacts with Bcl-2, Raf-1, steroid hormone receptors, Siah-1, and hepatocyte growth factor (HGF) receptors, implying multiple functions for the BAG-1 protein. Here, we provide evidence that gene transfer-mediated overexpression of BAG-1 markedly enhances the motility of human gastric cancer cells. Two independent in vitro migration assays showed that the BAG-1-expressing MKN74 cells exhibited more active migration compared with control transfectants or parent MKN74 cells. In MKN74 cells, the overexpression of BAG-1 affected neither cell adhesion capability nor migration responses to HGF. The promotive effect of BAG-1 on cell migration was similarly observed in transfectants of another human gastric cancer MKN45 cell line. In BAG-1 transfected gastric cancer MKN74 cells, BAG-1 colocalized with cytokeratin as well as actin filaments, and was concentrated at membrane ruffles induced by lysophosphatidic acid (LPA). Taken together, these studies demonstrate that BAG-1 has a novel function as promoter of cell migration in human gastric cancer cells, possibly through cooperation with cytoskeletal proteins.
journal_name
Oncogenejournal_title
Oncogeneauthors
Naishiro Y,Adachi M,Okuda H,Yawata A,Mitaka T,Takayama S,Reed JC,Hinoda Y,Imai Kdoi
10.1038/sj.onc.1202661subject
Has Abstractpub_date
1999-05-27 00:00:00pages
3244-51issue
21eissn
0950-9232issn
1476-5594journal_volume
18pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::Bladder cancer is one of the most common causes of death in industrialized countries. New tumor markers and therapeutic approaches are still needed to improve the management of bladder cancer patients. Choline kinase-alpha (ChoKalpha) is a metabolic enzyme that has a role in cell proliferation and transformation. Inhi...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2009.91
更新日期:2009-07-02 00:00:00
abstract::The efficacy of anticancer therapy is limited by the development of drug resistance. While the role of p53 in the intrinsic sensitivity of human cancer cells to paclitaxel (PTX) remains controversial, its role in acquired paclitaxel resistance has never been addressed. In this study we examined the p53 status of three...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203642
更新日期:2000-06-22 00:00:00
abstract::We describe the cytogenetic and molecular characterization of a t(1;13)(q22;q12) constitutional rearrangement occurring in a patient with a relatively benign form of neuroblastoma, called ganglioneuroblastoma. Somatic cell hybrids were generated between mouse 3T3 cells and a lymphoblastoid cell line from this patient,...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
abstract::Tbx3 is a member of the T-box family of transcription factors. Mutations in Tbx3 cause ulnar-mammary syndrome, an autosomal dominant disorder characterized by upper limb defects, apocrine-gland defects including mammary hypoplasia, and tooth, hair and genital defects. In cell culture, Tbx3 and its close relative Tbx2 ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205476
更新日期:2002-05-30 00:00:00
abstract::The LoVo colon carcinoma cell line that presents two wild type p53 alleles was used as the recipient for a series of transfections with p53 expression vectors coding for wild-type or three different mutants (143ala, 175his or 273his). The parental cell line as well as all clones that had rearranged the plasmid with co...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1996-02-15 00:00:00
abstract::Functional alterations or loss of tumor-suppressor genes are an important feature of neoplastic progression in humans. The employment of suitable animal model systems would greatly facilitate the detection and manipulation of such genes. We describe here an experimental approach to this problem based on the analysis o...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
abstract::Non-small cell lung cancer remains a highly lethal malignancy. Using the tamoxifen inducible Hnf1b:CreERT2 (H) transgenic mouse crossed to the LsL-KrasG12D (K) transgenic mouse, we recently discovered that an Hnf1b positive cell type in the lung is sensitive to adenoma formation when expressing a mutant KrasG12D allel...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-019-1031-z
更新日期:2020-01-01 00:00:00
abstract::Silencing of the MLH1 gene by promoter hypermethylation is the mechanism underlying the microsatellite instability (MSI) phenotype in endometrial cancers. However, the profile of CpG methylation in a wide range of MLH1 promoters in endometrial cancers and in the normal endometrium is largely unknown. The present study...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206365
更新日期:2003-04-17 00:00:00
abstract::Sam68 phosphorylation correlates with Fyn but not Lck expression in T cells. This substrate has been used here to explore the possible basis of the specificity of Fyn versus Lck. We show that this specificity is not based on a spatial segregation of the two kinases, since a chimeric Lck molecule containing the membran...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205929
更新日期:2002-10-17 00:00:00
abstract::Targeting Bruton tyrosine kinase (BTK) by ibrutinib is an effective treatment for patients with relapsed/refractory mantle cell lymphoma (MCL). However, both primary and acquired resistance to ibrutinib have developed in a significant number of these patients. A combinatory strategy targeting multiple oncogenic pathwa...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.155
更新日期:2016-12-01 00:00:00
abstract::Signal transducers and activators of transcription (Stats) are known to transduce signals from the cell surface to the nucleus in cytokine receptor signaling. We examined the capacity of platelet-derived growth factor (PDGF) receptor to interact with and activate Stat molecules. Activation of the PDGF beta-receptor le...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201555
更新日期:1998-01-29 00:00:00
abstract::Melanoma is the most aggressive form of skin cancer and is notoriously resistant to all current modalities of cancer therapy. A large set of genetic, functional and biochemical studies suggest that melanoma cells become 'bullet proof' against a variety of chemotherapeutic drugs by exploiting their intrinsic resistance...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1206454
更新日期:2003-05-19 00:00:00
abstract::We have studied the relationship between ras-GAP and G protein coupled receptors in a proliferative setting comprised of NIH3T3 expressing transfected muscarinic receptors (mAChRs). GAP expression plasmids were engineered to encode wild-type GAP, its carboxyl-terminal catalytic domain, a mutant lacking a portion of th...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
abstract::TNFα is a pleiotropic cytokine which fuels tumor cell growth, invasion, and metastasis in some malignancies, while in others it induces cytotoxic cell death. However, the molecular mechanism by which TNFα exerts its diverse effects on breast cancer subtypes remains elusive. Using in vitro assays and mouse xenografts, ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-018-0472-0
更新日期:2019-01-01 00:00:00
abstract::The protein kinase encoded by the ataxia telangiectasia and Rad3-related (ATR) gene is activated by DNA-damaging agents that are frequently used as anticancer therapeutics. Inhibition of ATR expression in cultured cancer cells has been demonstrated to increase sensitivity to chemotherapeutic drugs, including the DNA-c...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.624
更新日期:2011-06-02 00:00:00
abstract::beta-Catenin, a component of the Wnt signaling pathway, is a coactivator of human androgen receptor (hAR) transcriptional activity. Here, we show that Wnt signaling also influences androgen-mediated signaling through its ability to regulate hAR mRNA and protein in prostate cancer (PCa) cells. Three functional LEF-1/TC...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209366
更新日期:2006-06-08 00:00:00
abstract::Intrachromosomal rearrangements involving the RET and the adjacent H4 or ELE1 gene are very frequent events in thyroid cancer of children from Belarus after the Chernobyl reactor accident (Klugbauer et al., 1995). The fusion product between ELE1 and RET (RET/ PTC3) seems to be the prevailing type of rearrangement as s...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1996-09-05 00:00:00
abstract::p53 is known to play a role in iron homeostasis and is required for FDXR-mediated iron metabolism via iron regulatory protein 2 (IRP2). Interestingly, p53 is frequently mutated in tumors wherein iron is often accumulated, suggesting that mutant p53 may exert its gain of function by altering iron metabolism. In this st...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-019-0876-5
更新日期:2019-08-01 00:00:00
abstract::Radicicol, a macrocyclic anti-fungal antibiotic, has the ability to suppress transformation by diverse oncogenes such as Src, Ras and Mos. Despite this useful property, the mechanism by which radicicol exerts its anti-transformation effects is currently unknown. To understand the transformation-suppressing effects of ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201790
更新日期:1998-05-01 00:00:00
abstract::A mouse gene encoding a receptor tyrosine kinase, designated Embryonic receptor kinase (EmRK2), was isolated from embryoid bodies (EBs) generated by differentiating embryonic stem (ES) cells in culture for 6 days. Sequence analysis of EmRK2 cDNA clones predicts a receptor with a 755 amino acid extracellular region wit...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::The NAD-dependent deacetylase sirtuin 1 (SIRT1), a member of the mammalian sirtuin family, plays a pivotal role in deacetylating histone and nonhistone proteins. Recently, it has been reported that SIRT1 is upregulated in various kinds of tumors and is associated with cell growth and metastasis. However, the factors a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-020-1298-0
更新日期:2020-05-01 00:00:00
abstract::Oncogenic Ras is known to drive both the Rac and Raf-MAP-kinase pathways, which act in concert to cause cell transformation. Unlike the Raf-MAP-kinase cascade, however, the downstream elements of Rac pathway are not fully understood. Previously, we showed that cytosolic phospholipase A2 (cPLA2) and subsequent metaboli...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208151
更新日期:2004-12-09 00:00:00
abstract::c-Myc promotes cellular proliferation, sensitizes cells to apoptosis and prevents differentiation. It binds cyclin T1 structurally and functionally from the positive transcription elongation factor b (P-TEFb). The cyclin-dependent kinase 9 (Cdk9) in P-TEFb then phosporylates the C-terminal domain of RNA polymerase II,...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206800
更新日期:2003-08-28 00:00:00
abstract::A seven-transmembrane protein, frizzled, has been implicated in a planar cell polarity (PCP) pathway as well as the canonical Wnt signaling pathway. Although both pathways require a cytoplasmic protein, dishevelled, the molecular mechanism by which frizzled regulates intracellular signaling remains to be elucidated. I...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207817
更新日期:2004-08-12 00:00:00
abstract::An integrative functional genomics study of multiple forms of data are vital for discovering molecular drivers of cancer development and progression. Here, we present an integrated genomic strategy utilizing DNA methylation and transcriptome profile data to discover epigenetically regulated genes implicated in cancer ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.176
更新日期:2017-01-05 00:00:00
abstract::The breast cancer susceptibility gene 1 (BRCA1) is mutated in approximately 50% of hereditary breast cancers, and its expression is decreased in 30-40% of sporadic breast cancers, suggesting a general role in breast cancer development. BRCA1 physically and functionally interacts with estrogen receptor-alpha (ERalpha) ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2008.405
更新日期:2009-01-29 00:00:00
abstract::Observations that genome-wide DNA hypomethylation induces genome instability and tumors in animals caution against the indiscriminate use of demethylating agents, such as 5-aza-2'-deoxycytidine (5-Aza-dC). Using primary mouse embryonic fibroblasts harboring a lacZ mutational reporter construct that allows the quantifi...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.9
更新日期:2012-12-13 00:00:00
abstract::In previous studies we changed five conserved amino acid residues in the catalytic domain of the yeast Ras-specific guanine nucleotide exchange factor CDC25GEF (Park et al., 1994). One of the substitutions (R1489E) resulted in a molecule which could bind Ras but was catalytically inactive. These observations suggested...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1200893
更新日期:1997-02-20 00:00:00
abstract::Chromosomal translocations involving the Mixed-Lineage Leukaemia (MLL) gene underlie many human leukaemias and MLL rearrangements are found in both acute myelogenous and acute lymphoblastic leukaemias. To assess the functionally relevant haematopoietic cell contexts for MLL fusions to be tumorigenic, we have generated...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210818
更新日期:2008-03-20 00:00:00
abstract::We have cloned and sequenced the nov gene (novH) which is the homolog of the chicken nov proto-oncogene overexpressed in avian nephroblastomas. The novH gene is highly conserved and encodes a putative IGF-binding protein similar to that of chicken. We report that relative to autologous normal kidney expression of novH...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00