Abstract:
:1. Nitric oxide (NO) is thought to play a neuromodulatory role in the nervous system of vertebrate and invertebrate species. In the hornworm Manduca sexta, NO-mediated signaling has been implicated in behavioral and developmental processes, but its exact function in neurons is unknown. In this study, we identify specific neurons in the CNS of Manduca larvae that accumulate cGMP in response to treatment with NO donors in the presence of cGMP-phosphodiesterase inhibitors. Subsets of these neurons were identified as motoneuron-12 (MN12) and intersegmental motoneurons (ISMs), which innervate dorsal oblique muscles of the larvae. 2. To investigate the physiological role of NO-evoked increases in cGMP in these motoneurons we performed intracellular recordings; we found that application of NO donors caused an increase in neuronal excitability that was characterized by an increase in the spontaneous firing frequency. When action potentials and EPSPs were blocked, NO treatment evoked a depolarization of the resting membrane potential and a decrease in the measured input resistance in both MN12 and the ISMs. 3. Additional experiments with MN12 showed that treatment with the cGMP analogue, 8-Br-cGMP mimicked the NO effect on the resting potential and the input resistance. Furthermore, MN12 incubation with the NOS inhibitor, L-NNA, resulted in a small hyperpolarization of the resting potential and an increase in the input resistance, and incubation with the sGC inhibitor, ODQ blocked the NO-evoked depolarization of MN12. Finally, NO treatment during voltage clamping of MN12 evoked an inward positive current. 4. Taken together, these results suggest that NO can act as a "gain control" of neuronal excitability, which might have an important role in insect behavior.
journal_name
Cell Mol Neurobioljournal_title
Cellular and molecular neurobiologyauthors
Zayas RM,Trimmer BAdoi
10.1007/s10571-006-9091-3subject
Has Abstractpub_date
2007-03-01 00:00:00pages
191-209issue
2eissn
0272-4340issn
1573-6830journal_volume
27pub_type
杂志文章abstract::Levels of cyclic nucleotides and ornithine decarboxylase (ODC) activity were examined following the application of various kinds of stimuli to superior cervical sympathetic ganglia (SCG), nodose ganglia, and vagus nerve fibers excised from the rat. The level of cyclic GMP in the SCG rose rapidly to about 4.5- to 7.5-f...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00710944
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journal_title:Cellular and molecular neurobiology
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journal_title:Cellular and molecular neurobiology
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00769042
更新日期:1991-04-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-019-00734-z
更新日期:2020-04-01 00:00:00
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journal_title:Cellular and molecular neurobiology
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
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更新日期:1992-06-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-010-9642-5
更新日期:2011-04-01 00:00:00
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journal_title:Cellular and molecular neurobiology
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更新日期:1991-10-01 00:00:00
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journal_title:Cellular and molecular neurobiology
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doi:10.1007/s10571-009-9348-8
更新日期:2009-06-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1023/a:1020629818207
更新日期:1998-12-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00733789
更新日期:1987-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s10571-014-0083-4
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journal_title:Cellular and molecular neurobiology
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journal_title:Cellular and molecular neurobiology
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journal_title:Cellular and molecular neurobiology
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更新日期:2013-01-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00742977
更新日期:1986-03-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-020-00904-4
更新日期:2020-07-05 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/a:1007125413308
更新日期:2001-02-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
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更新日期:2019-03-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00742976
更新日期:1986-03-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
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更新日期:2019-03-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
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更新日期:2016-11-01 00:00:00