Abstract:
:Glial cells have various important roles in regulation of brain functions. For such events, extracellular nucleotides/P2 receptors have central roles. Although there have been huge amount of literature about activation of P2 receptors and glial functions, little is known about what happens in glia or the brain if glial P2 receptor is inhibited. Here we show that the inhibition of P2 receptors in astrocytes, the most abundant glial cells and cause a constitutive release of nucleotides, resulted in secretion of metalloproteinase-9 (MMP-9), a metal-dependent endopeptidase that degrades extracellular matrix molecules and is important in regulation of brain remodeling. When cultured astrocytes were treated with apyrase (ecto-nucleotidase), reactive blue 2 (P2 receptor antagonist), and pertussis toxin, they secreted MMP-9, suggesting that Gi-coupled P2Y receptor-mediated signals constitutively suppress the production of MMP-9. Among Gi-coupled P2Y receptors, we found that an inhibition of P2Y(14) receptor, a receptor for nucleotide-sugars such as UDP-glucose, is responsible for the production of MMP-9 by pharmacological and molecular biochemical analysis. As for the mechanisms, the inhibition of P2Y(14) receptors resulted in the release of tumor necrosis factor (TNF)-α which then acted on astrocytes to induce MMP-9. Taken together, our results suggest that the constitutive releases of nucleotide-sugars in astrocytes should play an important role in maintaining the normal status of the cell, through Gi-coupled P2Y(14) receptors, and when the signal is removed, the cells start to release TNF-α, which then acts on astrocytes in a feedback fashion to boost MMP-9 synthesis and secretion.
journal_name
Cell Mol Neurobioljournal_title
Cellular and molecular neurobiologyauthors
Kinoshita M,Nasu-Tada K,Fujishita K,Sato K,Koizumi Sdoi
10.1007/s10571-012-9869-4subject
Has Abstractpub_date
2013-01-01 00:00:00pages
47-58issue
1eissn
0272-4340issn
1573-6830journal_volume
33pub_type
杂志文章abstract::The present study was undertaken to evaluate whether estrogen deprivation might lead to mitochondrial alteration of hippocampal neurons of ovariectomized (OVX) rats, and to evaluate the protective effect of estrogen and phytoestrogen on the mitochondrial alteration. First, OVX rats were used to mimic the pathologic ch...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-008-9265-2
更新日期:2008-09-01 00:00:00
abstract::We have used the nervous system of the medicinal leech as a preparation to study the molecular basis of neural repair. The leech central nervous system, unlike mammalian CNS, can regenerate to restore function, and contains identified nerve cells of known function and connectivity. We have constructed subtractive cDNA...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-005-3151-y
更新日期:2005-03-01 00:00:00
abstract::Endothelins have been implicated in the regulation of cell proliferation, differentiation, and apoptosis, but the mechanisms of these complex events are not yet fully understood. Although the nuclear factor-kappaB (NF-kappaB) was shown to play a prominent role in the above processes, its participation in endothelin re...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/a:1015195803445
更新日期:2001-12-01 00:00:00
abstract::: 1. Glutaric acidemia type I (GA I) is a neurometabolic disorder caused by deficiency of glutaryl-CoA dehydrogenase, which leads to tissue accumulation of predominantly glutaric acid (GA) and also 3-hydroxyglutaric acid to a lesser amount. Affected patients usually present progressive cortical atrophy and acute stria...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-006-9134-9
更新日期:2007-06-01 00:00:00
abstract::Sulfur dioxide (SO2) is a common gas pollutant that is detrimental to many organs. Previous studies have shown that SO2 exposure is involved in neurotoxicity and increased risk of many brain disorders; however, our understanding of the mechanisms underlying SO2-induced cytotoxicity on neuronal cells remains elusive. T...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-014-0153-7
更新日期:2015-05-01 00:00:00
abstract::1. The effects of radiofrequency lesions of the ventral noradrenergic bundle (VNB) on monoamine and metabolite concentrations in several discrete areas of the rat hypothalamus were examined. Monoamines and metabolites were analyzed utilizing high-performance liquid chromatography coupled with electrochemical detection...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00733792
更新日期:1987-12-01 00:00:00
abstract::1. Several weeks after administration of 5,7-dihydroxytryptamine (5,7-DHT) to Aplysia, a dark pigmentation appears in serotonin-containing neurons, and this pigmentation allows visual identification of serotonergic neurons but does not appear to alter their physiology. 2. We have determined the distribution of labeled...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00734932
更新日期:1992-08-01 00:00:00
abstract::1. Sporadic Creutzfeldt-Jakob disease (CJD) is a rapidly progressive and fatal disease. Patients with CJD usually become akinetic mutism within approximately 6 months. In addition, clinical signs and symptoms at early stage of sporadic CJD may not be easy to distinguish from other neurodegenerative diseases by neurolo...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-006-9370-z
更新日期:2006-02-01 00:00:00
abstract::Astragalosides (AST) are reported to be neuroprotective in focal cerebral ischemic models in vivo. In this study, the direct effect of AST against oxygen and glucose deprivation (OGD) including neuronal injury and the underlying mechanisms in vitro were investigated. 5 h OGD followed by 24 h of reperfusion [adding bac...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-014-0059-4
更新日期:2014-08-01 00:00:00
abstract::1. Effects of 5-hydroxytryptamine (5-HT) on neuronal nicotinic acetylcholine (ACh) receptor channels were investigated by expressing cloned channel subunits in Xenopus oocytes. 2. When channels were expressed with a combination of alpha 3 and beta 4 subunits, 5-HT (10 to 300 microM) reversibly inhibited an inward curr...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF02071882
更新日期:1995-08-01 00:00:00
abstract::In chromaffin cells, SNARE proteins, forming the basic exocytotic machinery are present in membrane clusters of 500-600 nm in diameter. These microdomains containing both SNAP-25 and syntaxin-1 are dynamic and the expression of altered forms of SNAREs modifies not only their motion but also the mobility of the associa...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-010-9565-1
更新日期:2010-11-01 00:00:00
abstract::Neuropathic pain is diagnosed primarily by sensory dysfunction, which includes both spontaneous, and stimulus-evoked pain. Clinical evaluation highlights the disabilities which characterise this condition for most patients. Chronic constriction injury of the sciatic nerve (CCI) evokes sensory dysfunction characteristi...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-013-9962-3
更新日期:2013-10-01 00:00:00
abstract::Fractalkine (CX3CL1) is a potent inflammatory mediator of the central nervous system, which is expressed by neurons and regulates microglial functions by binding to fractalkine receptor (CX3CR1). It has been demonstrated that neuroinflammation plays an important role in iron accumulation of the brain leading to neuron...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-019-00694-4
更新日期:2019-10-01 00:00:00
abstract::The intention of this review is to emphasize the current knowledge about the extent and importance of the substances co-localized with magnocellular arginine vasopressin (AVP) and oxytocin (OXY) as potential candidates for the gradual clarification of their actual role in the regulation of hydromineral homeostasis. Ma...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s10571-008-9306-x
更新日期:2008-12-01 00:00:00
abstract::Intrachain disulfide bond formation among the cysteine thiols of SNAP-25, a component of the SNARE protein complex required for neurotransmitter release, has been hypothesized to link oxidative stress and inhibition of synaptic transmission. However, neither the availability in vivo of SNAP-25 thiols, which are known ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-011-9748-4
更新日期:2012-03-01 00:00:00
abstract::1. An identified dopaminergic interneuron (RPeD1) of the snail Lymnaea stagnalis, makes specific synaptic connections with a number of target (VI and VJ) but not non-target (VF and RPB) neurons in vivo. When cultured in vitro with both target and non-target cells, RPeD1 re-establishes synapses with target cells only. ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF02152058
更新日期:1996-10-01 00:00:00
abstract::The effects of the calcium channel blocker D-600 on the cation channels activated by acetylcholine (ACh) was studied in voltage-clamped Aplysia neurons by voltage-jump relaxation analysis. D-600 blocked the steady-state ACh current in a highly voltage-dependent manner, the degree of antagonism increasing with membrane...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00734714
更新日期:1983-12-01 00:00:00
abstract::Working memory (WM) is a highly heritable cognitive trait that is involved in many higher-level cognitive functions. In the past few years, much evidence has indicated that the reduction of dopamine activity in human brain can impair the WM system of the neuropsychiatric disorders. In this study, we hypothesized that ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-012-9817-3
更新日期:2012-08-01 00:00:00
abstract::Rosmarinic acid (RA) is a natural polyphenolic compound with a well-documented neuroprotective effect mainly associated with its anti-inflammatory and antioxidant activities. Recently, our research group developed and optimized chitosan-coated RA nanoemulsions (RA CNE) intended to be used for nasal delivery as a new p...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-019-00727-y
更新日期:2020-01-01 00:00:00
abstract::Oligodendrocyte precursor cell (OPC) maturation requires membrane addition for myelin sheath formation. Since the Rab system has been shown to contribute to membrane addition in other cell types, in this study, we explored the role of Rab in OPC maturation. SiRNA and shRNA techniques and conditional knockout mice prov...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-017-0465-5
更新日期:2017-10-01 00:00:00
abstract::1. Doubly transgenic mice were some differences in the period proceeding of the development of Abeta-42 deposits and behavioral deficits. It was not characterized human mutant PS2 (hPS2) with APPsw in the brains of double transgenic mice. The aim of this study was to examine whether doubly transgenic mice co-expressin...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-005-4950-x
更新日期:2005-08-01 00:00:00
abstract::MicroRNAs are small post-transcriptional regulators that modulate gene expression by directly interacting with their target transcripts. Since the interaction between miRNAs and target mRNAs does not require a perfect match, one single miRNA can influence the expression of several genes and lead to a very broad array ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-020-00836-z
更新日期:2021-01-01 00:00:00
abstract::Elevated iron levels in the substantia nigra (SN) participate in neuronal death in Parkinson's disease (PD), while the mechanisms underlying the increased iron are still unknown. Ceruloplasmin (CP), a ferroxidase, converts highly toxic ferrous iron to its non-toxic ferric form, which cooperated with ferroportin1 (FP1)...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-015-0161-2
更新日期:2015-07-01 00:00:00
abstract::1. Golgi-Kopsch preparations of the oral ventral nuclei of human thalamus were analyzed in an attempt to classify the neuronal types. 2. Three types of neurons are described for the first time in humans. Type I neurons are large or medium in size and bear dendrites with protrusions, spines, and short hair-like appenda...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00712927
更新日期:1992-06-01 00:00:00
abstract::The purpose of the study was to examine the nanoscale distribution and density of the VEGFR-2 membrane receptor on the endothelial cell surface of glioma microvasculature. Immunofluorescence and atomic force microscopy combined with immunogold labeling techniques were used to characterize and determine the position of...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-011-9665-6
更新日期:2011-07-01 00:00:00
abstract::Although it is known that brain-derived neurotrophic factor (BDNF) plays a critical role in neuronal survival and differentiation, its effect on lipid homeostasis is poorly understood. To understand them, we here investigated the effect of BDNF on the fatty acid composition of primary neurons. A detailed analysis of t...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-012-9863-x
更新日期:2012-11-01 00:00:00
abstract::1. In the retinal inner nuclear layer of the majority of species, a dopaminergic neuronal network has been visualized in either amacrine cells or the so-called interplexiform cells. 2. Binding studies of retinal dopamine receptors have revealed the existence of both D1- as well D2-subtypes. The D1-subtype was characte...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/BF00711177
更新日期:1990-09-01 00:00:00
abstract::Organotellurium compounds have been synthesized since 1840, but pharmacological and toxicological studies about them are still incipient. Therefore, the objective of this study was to verify the effect of acute administration of the organochalcogen 3-butyl-1-phenyl-2-(phenyltelluro)oct-en-1-one on some parameters of o...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-010-9547-3
更新日期:2010-10-01 00:00:00
abstract::Cannabinoid (CB) ligands have demonstrated neuroprotective properties. In this study we compared the effects of a diverse set of CB ligands against β amyloid-mediated neuronal toxicity and activated microglial-conditioned media-based neurotoxicity in vitro, and compared this with a capacity to directly alter β amyloid...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-013-9984-x
更新日期:2014-01-01 00:00:00
abstract::The original version of this article unfortunately contained a typo in co-author name. ...
journal_title:Cellular and molecular neurobiology
pub_type: 已发布勘误
doi:10.1007/s10571-020-00990-4
更新日期:2020-10-31 00:00:00