Characterization of ryanodine receptor-mediated calcium release in human B cells: relevance to diagnostic testing for malignant hyperthermia.


BACKGROUND:Mutations in the ryanodine type 1 receptor (RyR1) are causative for malignant hyperthermia. Studies in human B lymphocytes have shown that measurement of RyR1-mediated intracellular Ca(2+) (Ca(2+)(i)) release can differentiate between normal and malignant hyperthermia-susceptible individuals. The authors have further developed the B-cell assay by pharmacologically characterizing RyR1-mediated Ca release in two normal human B-cell lines and demonstrating increased sensitivity of lymphocytes to the RyR1 agonist 4-chloro-m-cresol (4-CmC) in the porcine model of MH. METHODS:Ca(2+)(i) was measured fluorometrically using fura-2 in populations of cells in suspension or with fluo-4 in single cells using confocal microscopy. The Dakiki and PP normal human B cell lines were used, as well as lymphocytes obtained from normal and malignant hyperthermia-susceptible pigs. 4-CmC was used to elicit RyR1-mediated Ca release; all experiments were performed in the absence of external Ca(2+). RESULTS:EC(50) values for 4-CmC were 0.98 and 1.04 mm for Dakiki and PP cells, respectively, demonstrating reproducibility. The 4-CmC-induced increase in Ca(2+)(i) was eliminated by thapsigargin and was unaffected by xestospongin C. The Ca(2+)(i) increase was separable from mitochondrial stores and was inhibited by azumolene. Caffeine did not induce Ca(2+)(i) release, but ryanodine depleted intracellular stores by 50%. Lymphocytes from pigs carrying the Arg614Cys mutation in RyR1 showed increased sensitivity to 4-CmC (EC(50) = 0.47 vs. 0.81 mm for cells derived from normal animals). CONCLUSIONS:RyR1-mediated Ca(2+) signals can be pharmacologically distinguished from other intracellular sources in human B cells, and alterations of RyR1 function can be successfully detected using Ca(2+) release from intracellular stores as an end point.






McKinney LC,Butler T,Mullen SP,Klein MG




Has Abstract


2006-06-01 00:00:00














  • Differential effects of fentanyl and morphine on intracellular Ca2+ transients and contraction in rat ventricular myocytes.

    abstract:BACKGROUND:Our objective was to elucidate the direct effects of fentanyl and morphine on cardiac excitation-contraction coupling using individual, field-stimulated rat ventricular myocytes. METHODS:Freshly isolated myocytes were loaded with fura-2 and field stimulated (0.3 Hz) at 28 degrees C. Amplitude and timing of ...


    pub_type: 杂志文章


    authors: Kanaya N,Zakhary DR,Murray PA,Damron DS

    更新日期:1998-12-01 00:00:00

  • Comparison of the effects of racemic bupivacaine, levobupivacaine, and ropivacaine on ventricular conduction, refractoriness, and wavelength: an epicardial mapping study.

    abstract:BACKGROUND:The study was designed to compare the effects of equimolar concentrations of racemic bupivacaine, levobupivacaine, and ropivacaine on ventricular conduction, anisotropy, duration and homogeneity of refractoriness, and wavelengths, and to provide a potency ratio for effects on conduction velocity. METHODS:Is...


    pub_type: 杂志文章


    authors: Aya AG,de la Coussaye JE,Robert E,Ripart J,Cuvillon P,Mazoit JX,Jeannes P,Fabbro-Péray P,Eledjam JJ

    更新日期:2002-03-01 00:00:00

  • Patient selection for day case-eligible surgery: identifying those at high risk for major complications.

    abstract:BACKGROUND:Due to economic pressures and improvements in perioperative care, outpatient surgical procedures have become commonplace. However, risk factors for outpatient surgical morbidity and mortality remain unclear. There are no multicenter clinical data guiding patient selection for outpatient surgery. The authors ...


    pub_type: 杂志文章


    authors: Mathis MR,Naughton NN,Shanks AM,Freundlich RE,Pannucci CJ,Chu Y,Haus J,Morris M,Kheterpal S

    更新日期:2013-12-01 00:00:00

  • A pharmacokinetic explanation for increasing recovery time following larger or repeated doses of nondepolarizing muscle relaxants.

    abstract::The authors used pharmacokinetic and pharmacodynamic modeling to explain the time course of neuromuscular blockade following single or multiple doses of three nondepolarizing muscle relaxants. Published and unpublished pharmacokinetic and pharmacodynamic data for 13 normal subjects were used to simulate the duration o...


    pub_type: 杂志文章


    authors: Fisher DM,Rosen JI

    更新日期:1986-09-01 00:00:00

  • Pharmacokinetics and protein binding of propofol in patients with cirrhosis.

    abstract::The pharmacokinetics and protein binding of propofol were studied in ten patients with cirrhosis and in ten control patients undergoing elective surgery. All patients received 2.5 propofol as an intravenous bolus injection for the induction of anesthesia. Whole blood propofol concentrations were measured at in...


    pub_type: 杂志文章


    authors: Servin F,Desmonts JM,Haberer JP,Cockshott ID,Plummer GF,Farinotti R

    更新日期:1988-12-01 00:00:00

  • Intrathecal sufentanil for labor analgesia does not cause a sympathectomy.

    abstract:BACKGROUND:Intrathecal sufentanil (ITS) is frequently used to provide analgesia during labor. Decreases in blood pressure and sensory changes in this circumstance suggest that ITS may have a local anesthetic effect and thus cause a sympathectomy. To determine whether ITS given to laboring women causes a sympathectomy, ...


    pub_type: 杂志文章


    authors: Riley ET,Walker D,Hamilton CL,Cohen SE

    更新日期:1997-10-01 00:00:00

  • Blood Purification and Mortality in Sepsis and Septic Shock: A Systematic Review and Meta-analysis of Randomized Trials.

    abstract:BACKGROUND:Sepsis and septic shock are severe inflammatory conditions related to high morbidity and mortality. We performed a systematic review with meta-analysis of randomized trials to assess whether extracorporeal blood purification reduces mortality in this setting. METHODS:Electronic databases were searched for p...


    pub_type: 杂志文章,meta分析


    authors: Putzu A,Schorer R,Lopez-Delgado JC,Cassina T,Landoni G

    更新日期:2019-09-01 00:00:00

  • Effects of perioperative dexmedetomidine infusion in patients undergoing vascular surgery. The Study of Perioperative Ischemia Research Group.

    abstract:BACKGROUND:Dexmedetomidine, a highly selective alpha 2-adrenergic agonist, increases perioperative hemodynamic stability in healthy patients but decreases blood pressure and heart rate. The goal of this study was to evaluate, in a preliminary manner, the hemodynamic effects of perioperatively administered dexmedetomidi...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Talke P,Li J,Jain U,Leung J,Drasner K,Hollenberg M,Mangano DT

    更新日期:1995-03-01 00:00:00

  • Electroacupuncture improves survival in rats with lethal endotoxemia via the autonomic nervous system.

    abstract:BACKGROUND:Recent advances have indicated a complex interplay between the autonomic nervous system and the innate immune system. Targeting neural networks for the treatment of sepsis is being developed as a therapeutic strategy. Because electroacupuncture at select acupoints can modulate activities of the autonomic ner...


    pub_type: 杂志文章


    authors: Song JG,Li HH,Cao YF,Lv X,Zhang P,Li YS,Zheng YJ,Li Q,Yin PH,Song SL,Wang HY,Wang XR

    更新日期:2012-02-01 00:00:00

  • Body position changes redistribute lung computed-tomographic density in patients with acute respiratory failure.

    abstract::Ten patients with parenchymal acute respiratory failure (ARF) underwent computed tomography (CT) scans while in the supine and prone positions. At equal levels of positive end-expiratory pressure, the authors measured the changes of CT density in dorsal and ventral basilar lung regions induced by the change of positio...


    pub_type: 杂志文章


    authors: Gattinoni L,Pelosi P,Vitale G,Pesenti A,D'Andrea L,Mascheroni D

    更新日期:1991-01-01 00:00:00

  • Lidocaine as an analgesic for experimental pain.

    abstract::The purpose of this study was to evaluate the analgesic contribution of intravenously administered lidocaine and to correlate it with blood levels of the drug. In a double-blind manner, 14 healthy male volunteers received saline solution or lidocaine, 0.2 per cent, at three increasingly greater rates of infusion on tw...


    pub_type: 杂志文章


    authors: Rowlingson JC,DiFazio CA,Foster J,Carron H

    更新日期:1980-01-01 00:00:00

  • Association of ethnicity with the minimum alveolar concentration of sevoflurane.

    abstract:BACKGROUND:Selective breeding produces animal strains with varying anesthetic sensitivity. It thus seems unlikely that various human ethnicities have identical anesthetic requirements. Therefore, the authors tested the hypothesis that the minimum alveolar concentration of sevoflurane differs significantly as a function...


    pub_type: 杂志文章,随机对照试验


    authors: Ezri T,Sessler D,Weisenberg M,Muzikant G,Protianov M,Mascha E,Evron S

    更新日期:2007-07-01 00:00:00

  • Effects of intramuscular clonidine on hemodynamic and plasma beta-endorphin responses to gynecologic laparoscopy.

    abstract::Ninety women undergoing gynecologic laparoscopy were randomly given clonidine 3 or 4.5 micrograms/kg or saline intramuscularly 45-60 min prior to induction of anesthesia. Anesthesia was induced with thiopental 3.5 mg/kg and maintained with 0.3% end-tidal isoflurane in nitrous oxide and oxygen. The laparoscopy did not ...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Aho M,Lehtinen AM,Laatikainen T,Korttila K

    更新日期:1990-05-01 00:00:00

  • Epidural clonidine treatment for refractory reflex sympathetic dystrophy.

    abstract:BACKGROUND:Intraspinally administered alpha 2-adrenergic agonists may relieve pain in sympathetically maintained pain (SMP) syndromes, such as reflex sympathetically dystrophy (RSD), by spinal, peripheral, and central nervous system actions. This study examined analgesic efficacy and side effects of epidurally administ...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Rauck RL,Eisenach JC,Jackson K,Young LD,Southern J

    更新日期:1993-12-01 00:00:00

  • Halothane and isoflurane decrease alveolar epithelial fluid clearance in rats.

    abstract:BACKGROUND:Active sodium transport is the primary mechanism that drives alveolar fluid clearance. In the current study, the effects of exposure to halothane and isoflurane on alveolar fluid clearance in rats were evaluated. METHODS:Rats were exposed to either halothane (0.4% for 6 h or 2% for 2 h) or isoflurane (0.6% ...


    pub_type: 杂志文章


    authors: Rezaiguia-Delclaux S,Jayr C,Luo DF,Saïdi NE,Meignan M,Duvaldestin P

    更新日期:1998-03-01 00:00:00

  • Inhibitory effect of glucocorticoids on human-cloned 5-hydroxytryptamine3A receptor expressed in xenopus oocytes.

    abstract:BACKGROUND:Methylprednisolone, dexamethasone, and other glucocorticoids have been found effective against nausea and vomiting induced by chemotherapy and surgery. Although the specific 5-hydroxytriptamine3 (5-HT3) receptor antagonists such as ondansetron and ramosetron are used as antiemetics, reports show that the use...


    pub_type: 杂志文章


    authors: Suzuki T,Sugimoto M,Koyama H,Mashimo T,Uchida I

    更新日期:2004-09-01 00:00:00

  • Neonatal patterns of breathing after cesarean section with or without epidural fentanyl.

    abstract::The pattern of breathing and lung mechanics were evaluated during the first 7 h of life in full-term healthy newborns delivered by cesarean section with bupivacaine epidural anesthesia, without (group 1) or with the addition of 100 micrograms of fentanyl (group 2). Respiration and oxyhemoglobin saturation (SpO2) were ...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Benlabed M,Dreizzen E,Ecoffey C,Escourrou P,Migdal M,Gaultier C

    更新日期:1990-12-01 00:00:00

  • Anesthetic mishaps: breaking the chain of accident evolution.

    abstract::Anesthesia and surgery are a risk for all, the healthy as well as the sick. While the prevention of adverse outcomes in healthy patients is paramount, enhancement of safety for critically ill patients is also essential, since they are more likely to suffer a SNO after a critical incident. Dangers originate from a vari...


    pub_type: 杂志文章


    authors: Gaba DM,Maxwell M,DeAnda A

    更新日期:1987-05-01 00:00:00

  • Isoflurane and nociception: spinal alpha2A adrenoceptors mediate antinociception while supraspinal alpha1 adrenoceptors mediate pronociception.

    abstract:BACKGROUND:The authors recently established that the analgesic actions of the inhalation anesthetic nitrous oxide were mediated by noradrenergic bulbospinal neurons and spinal alpha2B adrenoceptors. They now determined whether noradrenergic brainstem nuclei and descending spinal pathways are responsible for the antinoc...


    pub_type: 杂志文章


    authors: Kingery WS,Agashe GS,Guo TZ,Sawamura S,Davies MF,Clark JD,Kobilka BK,Maze M

    更新日期:2002-02-01 00:00:00

  • Comparison of bispectral index and composite auditory evoked potential index for monitoring depth of hypnosis in children.

    abstract:BACKGROUND:In pediatric patients, the Bispectral Index (BIS), derived from the electroencephalogram, and the composite A-Line autoregressive index (cAAI), derived from auditory evoked potentials and the electroencephalogram, have been used as measurements of depth of hypnosis during anesthesia. The performance and reli...


    pub_type: 杂志文章


    authors: Blussé van Oud-Alblas HJ,Peters JW,de Leeuw TG,Tibboel D,Klein J,Weber F

    更新日期:2008-05-01 00:00:00

  • Does the investing layer of the deep cervical fascia exist?

    abstract:BACKGROUND:The placement of the superficial cervical plexus block has been the subject of controversy. Although the investing cervical fascia has been considered as an impenetrable barrier, clinically, the placement of the block deep or superficial to the fascia provides the same effective anesthesia. The underlying me...


    pub_type: 杂志文章


    authors: Nash L,Nicholson HD,Zhang M

    更新日期:2005-11-01 00:00:00

  • Compliance with Surgical Care Improvement Project for Body Temperature Management (SCIP Inf-10) Is Associated with Improved Clinical Outcomes.

    abstract:BACKGROUND:In an effort to measure and improve the quality of perioperative care, the Surgical Care Improvement Project (SCIP) was introduced in 2003. The SCIP guidelines are evidence-based process measures designed to reduce preventable morbidity, but it remains to be determined whether SCIP-measure compliance is asso...


    pub_type: 杂志文章


    authors: Scott AV,Stonemetz JL,Wasey JO,Johnson DJ,Rivers RJ,Koch CG,Frank SM

    更新日期:2015-07-01 00:00:00

  • A randomized study of carbon dioxide management during hypothermic cardiopulmonary bypass.

    abstract::Eighty-six patients undergoing coronary artery bypass graft (n = 63) or intracardiac (n = 23) surgery were randomly assigned with respect to the target value for PaCO2 during cardiopulmonary bypass. In 44 patients the target PaCO2 was 40 mmHg, measured at the standard electrode temperature of 37 degrees C, while in 42...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Bashein G,Townes BD,Nessly ML,Bledsoe SW,Hornbein TF,Davis KB,Goldstein DE,Coppel DB

    更新日期:1990-01-01 00:00:00

  • Anesthetic influence on arteriolar diameters and tissue oxygen tension in hemorrhaged rats.

    abstract::Arteriolar diameters and tissue oxygen tensions were measured in the cremaster muscles of 68 hemorrhaged rats which were anesthetized with either intramuscular ketamine, 125 mg/kg, plus 30 mg/kg supplements as needed, or enflurane, 2.2% inspired. Animals breathed room air, or room air plus enflurane, throughout the ex...


    pub_type: 杂志文章


    authors: Longnecker DE,Ross DC,Silver IA

    更新日期:1982-09-01 00:00:00

  • Metabolism of ketamine stereoisomers by human liver microsomes.

    abstract::Ketamine is used clinically as a racemic mixture of optical isomers that differ in their analgesic properties and psychomimetic effects. Administered individually, or together as the racemate, ketamine enantiomers differ in their hepatic clearance and duration of anesthetic effect. S(+) ketamine exhibits a greater cle...


    pub_type: 杂志文章


    authors: Kharasch ED,Labroo R

    更新日期:1992-12-01 00:00:00

  • Soluble complement receptor type 1 inhibited the systemic organ injury caused by acid instillation into a lung.

    abstract:BACKGROUND:Acid aspiration into one lung causes contralateral lung injury and systemic organ injury; this injury is thought to be mediated by the sequestration of activated neutrophils. Recombinant human soluble complement receptor 1 (sCR1) inhibits both the classical and alternative complement pathways; this study inv...


    pub_type: 杂志文章


    authors: Nishizawa H,Yamada H,Miyazaki H,Ohara M,Kaneko K,Yamakawa T,Wiener-Kronish J,Kudoh I

    更新日期:1996-11-01 00:00:00

  • Factor VII levels and international normalized ratios in the early phase of warfarin therapy.

    abstract:BACKGROUND:Factor VII is the most affected clotting factor during the early phase of warfarin therapy. An international normalized ratio (INR) of more than 1.4 is considered unsafe for epidural catheter placement or removal, according to the American Society of Regional Anesthesia and Pain Medicine. The authors tested ...


    pub_type: 杂志文章


    authors: Benzon HT,Avram MJ,Benzon HA,Kirby-Nolan M,Nader A

    更新日期:2010-02-01 00:00:00

  • Days Alive and Out of Hospital: Validation of a Patient-centered Outcome for Perioperative Medicine.

    abstract:BACKGROUND:Days alive and out of hospital is a potentially useful patient-centered quality measure for perioperative care in adult surgical patients. However, there has been very limited prior validation of this endpoint with respect to its ability to capture differences in patient-level risk factor profiles and longer...


    pub_type: 杂志文章


    authors: Jerath A,Austin PC,Wijeysundera DN

    更新日期:2019-07-01 00:00:00

  • Anesthesia for creation of a forearm fistula in patients with endstage renal failure.

    abstract::The effects of local infiltration anesthesia, brachial plexus blockade, isoflurane, or halothane anesthesia on blood flow through the brachial artery and through a newly created forearm arteriovenous fistula (AVF) were compared in 36 patients with endstage renal failure. Brachial artery blood flow was measured at two ...


    pub_type: 杂志文章


    authors: Mouquet C,Bitker MO,Bailliart O,Rottembourg J,Clergue F,Montejo LS,Martineaud JP,Viars P

    更新日期:1989-06-01 00:00:00

  • "Gentlemen! This Is No Humbug": Did John Collins Warren, M.D., Proclaim These Words on October 16, 1846, at Massachusetts General Hospital, Boston?

    abstract::The proclamation, "Gentlemen! this is no humbug," attributed to John Collins Warren, M.D., was not identified in any contemporaneous eyewitness report of William T. G. Morton's October 16, 1846, demonstration of ether at Massachusetts General Hospital. The earliest known documentation of the proclamation is in Nathan ...


    pub_type: 传,历史文章,杂志文章


    authors: Haridas RP

    更新日期:2016-03-01 00:00:00