Characterization of ryanodine receptor-mediated calcium release in human B cells: relevance to diagnostic testing for malignant hyperthermia.


BACKGROUND:Mutations in the ryanodine type 1 receptor (RyR1) are causative for malignant hyperthermia. Studies in human B lymphocytes have shown that measurement of RyR1-mediated intracellular Ca(2+) (Ca(2+)(i)) release can differentiate between normal and malignant hyperthermia-susceptible individuals. The authors have further developed the B-cell assay by pharmacologically characterizing RyR1-mediated Ca release in two normal human B-cell lines and demonstrating increased sensitivity of lymphocytes to the RyR1 agonist 4-chloro-m-cresol (4-CmC) in the porcine model of MH. METHODS:Ca(2+)(i) was measured fluorometrically using fura-2 in populations of cells in suspension or with fluo-4 in single cells using confocal microscopy. The Dakiki and PP normal human B cell lines were used, as well as lymphocytes obtained from normal and malignant hyperthermia-susceptible pigs. 4-CmC was used to elicit RyR1-mediated Ca release; all experiments were performed in the absence of external Ca(2+). RESULTS:EC(50) values for 4-CmC were 0.98 and 1.04 mm for Dakiki and PP cells, respectively, demonstrating reproducibility. The 4-CmC-induced increase in Ca(2+)(i) was eliminated by thapsigargin and was unaffected by xestospongin C. The Ca(2+)(i) increase was separable from mitochondrial stores and was inhibited by azumolene. Caffeine did not induce Ca(2+)(i) release, but ryanodine depleted intracellular stores by 50%. Lymphocytes from pigs carrying the Arg614Cys mutation in RyR1 showed increased sensitivity to 4-CmC (EC(50) = 0.47 vs. 0.81 mm for cells derived from normal animals). CONCLUSIONS:RyR1-mediated Ca(2+) signals can be pharmacologically distinguished from other intracellular sources in human B cells, and alterations of RyR1 function can be successfully detected using Ca(2+) release from intracellular stores as an end point.






McKinney LC,Butler T,Mullen SP,Klein MG




Has Abstract


2006-06-01 00:00:00














  • Nitrogen analyzer adaptation for use in the presence of halogenated anesthetics.

    abstract::Interposition of activated carbon between a nitrogen analyzer needle valve and gas mixtures containing halothane, methoxyflurane, enflurane, or isoflurane eliminated the interfering effects of these agents, but added sampling delays. Placement of a carbon-filled Swinney adaptor in the sampling line between the needle ...


    pub_type: 杂志文章


    authors: Gilmour IJ,Holland R,Craig DB

    更新日期:1975-12-01 00:00:00

  • The effect of 30% nitrous oxide on thermoregulatory responses in humans during hypothermia.

    abstract::Clinical studies have reported that body core temperature decreases during prolonged surgery and anesthesia. Although this finding has been attributed primarily to increased heat loss resulting from exposure of body cavities and infusion of cold solutions, it is generally recognized that anesthesia interferes with the...


    pub_type: 杂志文章


    authors: Passias TC,Mekjavić IB,Eiken O

    更新日期:1992-04-01 00:00:00

  • Interaction of neuromuscular blocking effects of neomycin and polymyxin B.

    abstract::Neomycin and polymyxin B produce neuromuscular blocks with distinct features by different mechanisms of action. In eight anesthetized cats the authors studied their interaction by examining the neuromuscular block produced by an equipotent mixture. Values of onset, potency, dose-response relations, duration and revers...


    pub_type: 杂志文章


    authors: Lee C,de Silva AJ

    更新日期:1979-03-01 00:00:00

  • Extubation Success Prediction in a Multicentric Cohort of Patients with Severe Brain Injury.

    abstract:BACKGROUND:Patients with brain injury are at high risk of extubation failure. METHODS:We conducted a prospective observational cohort study in four intensive care units of three university hospitals. The aim of the study was to create a score that could predict extubation success in patients with brain injury. RESULT...


    pub_type: 杂志文章,多中心研究


    authors: Asehnoune K,Seguin P,Lasocki S,Roquilly A,Delater A,Gros A,Denou F,Mahé PJ,Nesseler N,Demeure-Dit-Latte D,Launey Y,Lakhal K,Rozec B,Mallédant Y,Sébille V,Jaber S,Le Thuaut A,Feuillet F,Cinotti R,ATLANREA group.

    更新日期:2017-08-01 00:00:00

  • Ventilator-associated pneumonia leading to acute lung injury after trauma: importance of Haemophilus influenzae.

    abstract:BACKGROUND:Ventilator-associated pneumonia is a clear risk factor for acute lung injury which has been poorly described in trauma patients. This prospective study was undertaken to estimate the incidence of such ventilator-associated pneumonia leading to acute lung injury, the risk factors, and the associated morbidity...


    pub_type: 杂志文章


    authors: Stéphan F,Mabrouk N,Decailliot F,Delclaux C,Legrand P

    更新日期:2006-02-01 00:00:00

  • Physiologic and antinociceptive effects of intrathecal resiniferatoxin in a canine bone cancer model.

    abstract:BACKGROUND:Resiniferatoxin is a potent capsaicin analog. Intrathecal administration leads to selective, prolonged opening of the transient receptor potential V1 ion channel, which is localized mainly to C-fiber primary afferent nociceptive sensory neurons. Following work in laboratory animals, the authors explored the ...


    pub_type: 杂志文章


    authors: Brown DC,Iadarola MJ,Perkowski SZ,Erin H,Shofer F,Laszlo KJ,Olah Z,Mannes AJ

    更新日期:2005-11-01 00:00:00

  • Interaction of anesthesia, beta-receptor blockade, and blood loss in dogs with induced myocardial infarction.

    abstract::The cardiovascular effects of halothane-nitrous oxide anesthesia, and beta-receptor blockade with either propranolol or practolol, were studied in 15 dogs in which severe myocardial infarction had been induced ten days earlier. The hemodynamic responses to blood loss amounting to 25 per cent of estimated blood volume,...


    pub_type: 杂志文章


    authors: Prys-Roberts C,Roberts JG,Foëx P,Clarke TN,Bennett MJ,Ryder WA

    更新日期:1976-09-01 00:00:00

  • Effects of intravascular volume replacement on lung and kidney function and damage in nonseptic experimental lung injury.

    abstract:BACKGROUND:Intravascular volume replacement is often required in the presence of increased pulmonary capillary leakage, for example in patients with volutrauma with major hemorrhage. In the present study, the effects of Ringer's acetate (RA), gelatin-polysuccinate (GEL), and a modern hydroxyethyl starch (HES, 6% 130/0....


    pub_type: 杂志文章


    authors: Silva PL,Güldner A,Uhlig C,Carvalho N,Beda A,Rentzsch I,Kasper M,Wiedemann B,Spieth PM,Koch T,Capelozzi VL,Pelosi P,Rocco PR,de Abreu MG

    更新日期:2013-02-01 00:00:00

  • Cardiorespiratory effects of positive end-expiratory pressure during progressive tidal volume reduction (permissive hypercapnia) in patients with acute respiratory distress syndrome.

    abstract:BACKGROUND:In patients with acute respiratory distress syndrome (ARDS), the ventilatory approach is based on tidal volume (VT) of 10-15 ml/kg and positive end-expiratory pressure (PEEP). To avoid further pulmonary injury, decreasing VT and allowing PaCO2 to increase (permissive hypercapnia) has been suggested. Effects ...


    pub_type: 杂志文章


    authors: Ranieri VM,Mascia L,Fiore T,Bruno F,Brienza A,Giuliani R

    更新日期:1995-10-01 00:00:00

  • Neuromuscular and cardiovascular effects of mivacurium in children.

    abstract::The neuromuscular and cardiovascular effects of mivacurium chloride (BW B1090U) were evaluated in 90 children (2-12 yr) during N2O:O2 halothane or N2O:O2 narcotic anesthesia. Neuromuscular response was evaluated by recording the force of contraction of the adductor of the thumb during train-of-four stimulation at 0.1 ...


    pub_type: 杂志文章


    authors: Goudsouzian NG,Alifimoff JK,Eberly C,Smeets R,Griswold J,Miler V,McNulty BF,Savarese JJ

    更新日期:1989-02-01 00:00:00

  • Effectiveness of triazolam, diazepam, and placebo as preanesthetic medications.

    abstract::Eighty-three ASA Physical Status 1-2 patients were orally premedicated with triazolam (0.125, 0.25, or 0.5 mg), diazepam (5, 10, or 15 mg), or placebo to evaluate the effectiveness of these drugs and doses in reducing preoperative anxiety, providing sedation, and producing amnesia. The drug treatments were administere...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Baughman VL,Becker GL,Ryan CM,Glaser M,Abenstein JP

    更新日期:1989-08-01 00:00:00

  • Amitriptyline for prolonged cutaneous analgesia in the rat.

    abstract:BACKGROUND:Amitriptyline has been reported to be a more potent local anesthetic than bupivacaine. In keeping with the objective of identifying drugs for prolonged cutaneous analgesia, the authors compared the cutaneous analgesic effectiveness of amitriptyline and bupivacaine in rats. METHODS:Rats were subcutaneously i...


    pub_type: 杂志文章


    authors: Khan MA,Gerner P,Kuo Wang G

    更新日期:2002-01-01 00:00:00

  • Postoperative pain facilitates nonthermoregulatory tremor.

    abstract:BACKGROUND:Spontaneous tremor is relatively common in normothermic patients after operation and has been attributed to many causes. The hypothesis that nonthermoregulatory shivering-like tremor is facilitated by postoperative pain was tested. In addition, the effects of intravenous lidocaine on nonthermoregulatory trem...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Horn EP,Schroeder F,Wilhelm S,Sessler DI,Standl T,von dem Busche K,Schulte am Esch J

    更新日期:1999-10-01 00:00:00

  • Carcinogen bioassay of enflurane in mice.

    abstract::A carcinogen bioassay of enflurane was performed in Swiss/ICR mice. Two groups of weanling mice, each of 125 males and 125 females, were exposed to eigher a maximum tolerated dose of enflurane, 3000 ppm (0.3 per cent v/v) or compressed air for 4 hs per day, 5 days per week. After 52 weeks exposure, 25 males and 25 fem...


    pub_type: 杂志文章


    authors: Baden JM,Egbert B,Mazze RI

    更新日期:1982-01-01 00:00:00

  • Cardiovascular responses to the induction of mild hypothermia in the presence of epidural anesthesia.

    abstract:BACKGROUND:The combining of epidural anesthesia with general anesthesia impairs central and peripheral thermoregulatory control and therefore is often accompanied by unintended intraoperative hypothermia. However, little is known about the cardiovascular response to hypothermia during combined epidural and general anes...


    pub_type: 杂志文章


    authors: Yoshida M,Shibata K,Itoh H,Yamamoto K

    更新日期:2001-04-01 00:00:00

  • A novel porcine model of ventilator-associated pneumonia caused by oropharyngeal challenge with Pseudomonas aeruginosa.

    abstract:BACKGROUND:Animal models of ventilator-associated pneumonia (VAP) in primates, sheep, and pigs differ in the underlying pulmonary injury, etiology, bacterial inoculation methods, and time to onset. The most common ovine and porcine models do not reproduce the primary pathogenic mechanism of the disease, through the asp...


    pub_type: 杂志文章


    authors: Li Bassi G,Rigol M,Marti JD,Saucedo L,Ranzani OT,Roca I,Cabanas M,Muñoz L,Giunta V,Luque N,Rinaudo M,Esperatti M,Fernandez-Barat L,Ferrer M,Vila J,Ramirez J,Torres A

    更新日期:2014-05-01 00:00:00

  • Effect of Exogenous Albumin on the Incidence of Postoperative Acute Kidney Injury in Patients Undergoing Off-pump Coronary Artery Bypass Surgery with a Preoperative Albumin Level of Less Than 4.0 g/dl.

    abstract:BACKGROUND:Hypoalbuminemia may increase the risk of acute kidney injury (AKI). The authors investigated whether the immediate preoperative administration of 20% albumin solution affects the incidence of AKI after off-pump coronary artery bypass surgery. METHODS:In this prospective, single-center, randomized, parallel-...


    pub_type: 杂志文章,随机对照试验


    authors: Lee EH,Kim WJ,Kim JY,Chin JH,Choi DK,Sim JY,Choo SJ,Chung CH,Lee JW,Choi IC

    更新日期:2016-05-01 00:00:00

  • The medical malpractice problem, and some possible solutions.

    abstract::The solutions to the problems of medical malpractice and malpractice insurance coverage are primarily in the hands of state legislatures. Legislation enacted has been primarily palliative, to assure continued availability of professional liability insurance. Unfortunately, no limit can be placed on the costs of such c...


    pub_type: 杂志文章


    authors: Dornette WH

    更新日期:1976-03-01 00:00:00

  • Isozyme-specific effects of protein kinase C in pain modulation.

    abstract:BACKGROUND:Protein kinase C (PKC) is a family of serine/threonine kinases that contains more than 10 isozymes. Evidence suggests that PKC may play important roles in pain modulation, but the isozyme-specific effects of PKC on different aspects of pain modulation are not fully understood. We hypothesize that different P...


    pub_type: 杂志文章


    authors: Zhao C,Leitges M,Gereau RW 4th

    更新日期:2011-12-01 00:00:00

  • Acute lung injury after instillation of human breast milk or infant formula into rabbits' lungs.

    abstract:BACKGROUND:Recent interest in shortening the fasting interval after ingestion of milk products demonstrated large volumes of breast milk in the stomach 2 h after breastfeeding. Although aspiration is a rare event, if it were to occur with human breast milk, it is important to understand the extent of the lung injury th...


    pub_type: 杂志文章


    authors: O'Hare B,Lerman J,Endo J,Cutz E

    更新日期:1996-06-01 00:00:00

  • Effects of succinylcholine and d-tubocurarine on epinephrine-induced arrhythmias during halothane anesthesia in dogs.

    abstract::The effects of subparalytic doses of succinylcholine and d-tubocurarine on epinephrine-induced cardiac arrhythmias during halothane anesthesia were evaluated in dogs. Succinylcholine markedly increased (P is less than .05) the arrhythmogenicity of epinephrine and d-tubocurarine slightly decreased (P is less than .05) ...


    pub_type: 杂志文章


    authors: Tucker WK,Munson ES

    更新日期:1975-01-01 00:00:00

  • Effect of 1.5% Topical Diclofenac on Clinical Neuropathic Pain.

    abstract:BACKGROUND:Neuropathic pain is a condition resulting from injury to the peripheral and/or central nervous system. Despite extensive research over the last several decades, neuropathic pain remains difficult to manage. METHODS:The authors conducted a randomized, placebo-controlled, double-blinded, and crossover clinica...


    pub_type: 杂志文章,随机对照试验


    authors: Ahmed SU,Zhang Y,Chen L,Cohen A,St Hillary K,Vo T,Houghton M,Mao J

    更新日期:2015-07-01 00:00:00

  • Beneficial effects of short-term vasopressin infusion during severe septic shock.

    abstract:BACKGROUND:Septic shock is associated with vasopressin deficiency and a hypersensitivity to its exogenous administration. The goal of the current study was to determine whether short-term vasopressin infusion in patients experiencing severe septic shock has a vasopressor sparing effect while maintaining hemodynamic sta...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Patel BM,Chittock DR,Russell JA,Walley KR

    更新日期:2002-03-01 00:00:00

  • Gabapentin suppresses cutaneous hyperalgesia following heat-capsaicin sensitization.

    abstract:BACKGROUND:The anticonvulsant gabapentin, proven effective for neuropathic pain in two large, placebo-controlled clinical trials, is widely used for treatment of chronic pain. Preclinical studies have demonstrated analgesic and antiallodynic effects in models involving neuronal sensitization and nerve injury, without a...


    pub_type: 杂志文章


    authors: Dirks J,Petersen KL,Rowbotham MC,Dahl JB

    更新日期:2002-07-01 00:00:00

  • Helium induces preconditioning in human endothelium in vivo.

    abstract:AIMS:Helium protects myocardium by inducing preconditioning in animals. We investigated whether human endothelium is preconditioned by helium inhalation in vivo. METHODS AND RESULTS:Forearm ischemia-reperfusion (I/R) in healthy volunteers (each group n = 10) was performed by inflating a blood pressure cuff for 20 min....


    pub_type: 杂志文章


    authors: Smit KF,Oei GT,Brevoord D,Stroes ES,Nieuwland R,Schlack WS,Hollmann MW,Weber NC,Preckel B

    更新日期:2013-01-01 00:00:00

  • Drug actions at mammalian motor nerve endings: the suppression of neostigmine-induced fasciculations by vecuronium and isoflurane.

    abstract::The occurrence of fasciculations following administration of agents is a well-known pharmacologic phenomenon. Using the cat soleus nerve-muscle preparation, intravenous neostigmine doses between 20-200 micrograms/kg evoked fasciculations in a dose-related manner. The data demonstrate that the fasciculations were the r...


    pub_type: 杂志文章


    authors: Baker T,Stanec A

    更新日期:1987-12-01 00:00:00

  • The pharmacokinetics and hemodynamic effects of intravenous and intramuscular dexmedetomidine hydrochloride in adult human volunteers.

    abstract:BACKGROUND:Dexmedetomidine is an alpha 2 agonist with potential utility in clinical anesthesia for both its sedative and sympatholytic properties. METHODS:The pharmacokinetics and hemodynamic changes that occurred in ten healthy male volunteers were determined after administration of dexmedetomidine 2 micrograms/kg by...


    pub_type: 杂志文章


    authors: Dyck JB,Maze M,Haack C,Vuorilehto L,Shafer SL

    更新日期:1993-05-01 00:00:00

  • Cumulative duration of "triple low" state of low blood pressure, low bispectral index, and low minimum alveolar concentration of volatile anesthesia is not associated with increased mortality.

    abstract:BACKGROUND:Mortality after noncardiac surgery has been associated with the "triple low state," a combination of low mean arterial blood pressure (<75 mmHg), low bispectral index (<45), and low minimum alveolar concentration of volatile anesthesia (<0.70). The authors set out to determine whether duration of a triple lo...


    pub_type: 杂志文章


    authors: Kertai MD,White WD,Gan TJ

    更新日期:2014-07-01 00:00:00

  • The effects of extracellular magnesium on myoplasmic [Ca2+] in malignant hyperthermia susceptible swine.

    abstract::It is now well established that the pathophysiology of the malignant hyperthermia (MH) syndrome is related to a malfunction of intracellular calcium homeostasis. Magnesium plays important roles in the basic contractile properties of muscle, and many of its actions are antagonistic to those of calcium. The aim of this ...


    pub_type: 杂志文章


    authors: Lopez JR,Sanchez V,Lopez I,Ryan JF,Mendoza M,Sreter FA,Allen PD

    更新日期:1990-07-01 00:00:00

  • Hepatic plasma flow during sodium nitroprusside-induced hypotension in humans.

    abstract::Changes in hepatic plasma flow (HPF) during sodium nitroprusside (SNP) induced hypotension were studied in 14 patients undergoing intracranial aneurysm surgery under neurolept anesthesia. Patients were monitored with the use of a radial artery catheter and a thermistor-tipped Swan-Ganz catheter. Hypotension was induce...


    pub_type: 杂志文章


    authors: Chauvin M,Bonnet F,Montembault C,Lafay M,Curet P,Viars P

    更新日期:1985-09-01 00:00:00