GM-CSF-surface-modified B16.F10 melanoma cell vaccine.


:Through the specific and tight interaction between streptavidin and biotin, a novel platform was developed to allow for rapid (less than 2h), efficient and durable display of streptavidin-tagged bioactive GM-CSF on the surface of biotinylated B16.F10 tumor cells. This technology involved biotinylation of the cell membrane with a biotin derivative and surface modification of the biotinylated cells with the bi-functional fusion protein, streptavidin-tagged GM-CSF. Furthermore, the resultant GM-CSF-modified B16.F10 whole tumor cell vaccine could induce strong and long-lasting systemic protection against the wild-type tumor challenge. Therefore, the platform may represent a fast, efficient and safe approach for the whole tumor cell vaccination.






Gao J,Huang S,Li M,Luo R,Wang X,Takashima A




Has Abstract


2006-06-19 00:00:00
















  • Methods for systematic reviews of administrative database studies capturing health outcomes of interest.

    abstract::This report provides an overview of methods used to conduct systematic reviews for the US Food and Drug Administration (FDA) Mini-Sentinel project, which is designed to inform the development of safety monitoring tools for FDA-regulated products including vaccines. The objective of these reviews was to summarize the l...


    pub_type: 杂志文章,评审


    authors: McPheeters ML,Sathe NA,Jerome RN,Carnahan RM

    更新日期:2013-12-30 00:00:00

  • Clinical and serological responses following primary and booster immunization with Salmonella typhi Vi capsular polysaccharide vaccines.

    abstract::Clinical and serum antibody responses following intramuscular injection of two formulations of Salmonella typhi Vi capsular polysaccharide (Vi) were assessed in a double-blind evaluation. Healthy adults were randomly assigned to receive a 25 micrograms dose of liquid (Vi-Liq; n = 182) or freeze-dried Vi vaccine (Vi-Ly...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Keitel WA,Bond NL,Zahradnik JM,Cramton TA,Robbins JB

    更新日期:1994-01-01 00:00:00

  • Placebo use in vaccine trials: recommendations of a WHO expert panel.

    abstract::Vaccines are among the most cost-effective interventions against infectious diseases. Many candidate vaccines targeting neglected diseases in low- and middle-income countries are now progressing to large-scale clinical testing. However, controversy surrounds the appropriate design of vaccine trials and, in particular,...


    pub_type: 杂志文章


    authors: Rid A,Saxena A,Baqui AH,Bhan A,Bines J,Bouesseau MC,Caplan A,Colgrove J,Dhai A,Gomez-Diaz R,Green SK,Kang G,Lagos R,Loh P,London AJ,Mulholland K,Neels P,Pitisuttithum P,Sarr SC,Selgelid M,Sheehan M,Smith PG

    更新日期:2014-08-20 00:00:00

  • Intranasal but not subcutaneous vaccination with LaAg allows rapid expansion of protective immunity against cutaneous leishmaniasis.

    abstract::Mucosal but not parenteral vaccination with whole Leishmania amazonensis promastigotes antigens (LaAg) is known to increase host resistance to infection by an as yet unknown immune mechanism. Since early immune responses are critical for infection establishment, in the present study the differential responses elicited...


    pub_type: 杂志文章


    authors: Pereira Silva Bezerra I,Amaral Abib M,Rossi-Bergmann B

    更新日期:2018-04-25 00:00:00

  • Rationale and design of a community-based double-blind randomized clinical trial of an HPV 16 and 18 vaccine in Guanacaste, Costa Rica.

    abstract::We report the rationale, design, methods and details of participation of a community-based, double-blind, randomized clinical trial of an HPV 16 and 18 vaccine conducted in two provinces of Costa Rica to investigate the efficacy and population impact of the vaccine in the prevention of cervical cancer precursors. More...


    pub_type: 杂志文章,随机对照试验


    authors: Herrero R,Hildesheim A,Rodríguez AC,Wacholder S,Bratti C,Solomon D,González P,Porras C,Jiménez S,Guillen D,Morales J,Alfaro M,Cyr J,Morrisey K,Estrada Y,Cortés B,Morera LA,Freer E,Schussler J,Schiller J,Lowy D,S

    更新日期:2008-09-02 00:00:00

  • Utility of the Sindbis replicon system as an Env-targeted HIV vaccine.

    abstract::Sindbis replicon-based vaccine vectors are designed to combine the immunostimulatory properties of replicating viruses with the superior safety profile of non-replicating systems. In this study we performed a detailed assessment of Sindbis (SIN) replicon vectors expressing HIV-1 envelope protein (Env) for the inductio...


    pub_type: 杂志文章


    authors: Center RJ,Miller A,Wheatley AK,Campbell SM,Siebentritt C,Purcell DF

    更新日期:2013-04-26 00:00:00

  • A global perspective of vaccination of healthcare personnel against measles: systematic review.

    abstract::Measles transmission has been well documented in healthcare facilities. Healthcare personnel who are unvaccinated and who lack other evidence of measles immunity put themselves and their patients at risk for measles. We conducted a systematic literature review of measles vaccination policies and their implementation i...


    pub_type: 杂志文章,评审


    authors: Fiebelkorn AP,Seward JF,Orenstein WA

    更新日期:2014-08-27 00:00:00

  • Effect of complement Factor H on anti-FHbp serum bactericidal antibody responses of infant rhesus macaques boosted with a licensed meningococcal serogroup B vaccine.

    abstract::FHbp is a major serogroup B meningococcal vaccine antigen. Binding of complement Factor H (FH) to FHbp is specific for human and some non-human primate FH. In previous studies, FH binding to FHbp vaccines impaired protective anti-FHbp antibody responses. In this study we investigated anti-FHbp antibody responses to a ...


    pub_type: 杂志文章


    authors: Giuntini S,Beernink PT,Granoff DM

    更新日期:2015-12-16 00:00:00

  • Indicators of influenza and pneumococcal vaccination in French nursing home residents in 2011.

    abstract:INTRODUCTION:Older adults living in nursing homes (NH) are at high risk of developing influenza and pneumococcal infections. The objectives of this study were to describe vaccination coverage for influenza and pneumococcal among French NH residents and to investigate which NH structure- and organisation-related aspects...


    pub_type: 杂志文章


    authors: de Souto Barreto P,Lapeyre-Mestre M,Vellas B,Rolland Y

    更新日期:2014-02-07 00:00:00

  • Efficient influenza B virus propagation due to deficient interferon-induced antiviral activity in MDCK cells.

    abstract::Influenza B virus infections are mainly restricted to humans, which is partially caused by the inability of influenza B virus NS1 protein to counteract the innate immune response of other species. However, for cell culture-based influenza vaccine production non-human cells, such as Madin-Darby canine kidney (MDCK) cel...


    pub_type: 杂志文章


    authors: Frensing T,Seitz C,Heynisch B,Patzina C,Kochs G,Reichl U

    更新日期:2011-09-22 00:00:00

  • Cross-reactive immune responses in mice after genetic vaccination with cDNA encoding hantavirus nucleocapsid proteins.

    abstract::Hantaviruses cause hemorrhagic fever with renal syndrome (HFRS) in about 150,000 individuals in Eurasia, and several hundred cases of hantavirus pulmonary syndrome (HPS) on the American continent annually. There is consequently a need for rapid diagnostics and effective prevention of hantaviral infections. In this stu...


    pub_type: 杂志文章


    authors: Lindkvist M,Lahti K,Lilliehöök B,Holmström A,Ahlm C,Bucht G

    更新日期:2007-02-19 00:00:00

  • Viral interference induced by live attenuated virus vaccine (OPV) can prevent otitis media.

    abstract:BACKGROUND:The goal of this study was to evaluate whether a live attenuated poliovirus vaccine (OPV) has clinically relevant interfering effect with non-polio infections causing otitis media in young children. METHODS:Open trial in which the intervention group (64 children) received OPV at the age of 2, 3, 6 and 12 mo...


    pub_type: 杂志文章


    authors: Seppälä E,Viskari H,Hoppu S,Honkanen H,Huhtala H,Simell O,Ilonen J,Knip M,Hyöty H

    更新日期:2011-11-03 00:00:00

  • Altered proportions of circulating CXCR5+ helper T cells do not dampen influenza vaccine responses in children with rheumatic disease.

    abstract::Biological therapy options for the treatment of rheumatic disease target molecules that can affect the cross-talk between innate and adaptive immune responses upon vaccination. Influenza vaccination in children with rheumatic disease has been recommended, but there are only sparse data on the quality of vaccine respon...


    pub_type: 杂志文章


    authors: Laestadius Å,Ingelman-Sundberg HM,Myrberg IH,Verme A,Sundberg E,Schweiger B,Saghafian-Hedengren S,Nilsson A

    更新日期:2019-06-19 00:00:00

  • Pathogenicity and immunogenicity of recombinant Tiantan Vaccinia Virus with deleted C12L and A53R genes.

    abstract::Interest is increasing regarding replicating poxvirus as HIV vaccine vector. In China, the Tiantan Vaccinia Virus (TV) has been used most extensively in the battle of eradicating smallpox. Recently, TV was developing as vaccine vector to fight against infectious diseases such as human immunodeficiency virus (HIV). How...


    pub_type: 杂志文章


    authors: Dai K,Liu Y,Liu M,Xu J,Huang W,Huang X,Liu L,Wan Y,Hao Y,Shao Y

    更新日期:2008-09-15 00:00:00

  • Cost-effectiveness analysis of the introduction of rotavirus vaccine in Iran.

    abstract:BACKGROUND:Although the mortality from diarrheal diseases has been decreasing dramatically in Iran, it still represents an important proportion of disease burden in children <5 years old. Rotavirus vaccines are among the most effective strategies against diarrheal diseases in specific epidemiological conditions. This s...


    pub_type: 杂志文章


    authors: Javanbakht M,Moradi-Lakeh M,Yaghoubi M,Esteghamati A,Mansour Ghanaie R,Mahmoudi S,Shamshiri AR,Zahraei SM,Baxter L,Shakerian S,Chaudhri I,Fleming JA,Munier A,Baradaran HR

    更新日期:2015-05-07 00:00:00

  • Frequency of IFN-gamma producing cells correlates with adjuvant enhancement of bacille Calmette-Guèrin induced protection against Mycobacterium bovis.

    abstract::Tuberculosis caused by infection with Mycobacterium tuberculosis or Mycobacterium bovis remains one of the most important infectious diseases of man and animals. The current vaccine M. bovis Calmette-Guérin (BCG) demonstrates variable efficacy and so a more robust strategy to either replace, or more likely supplement ...


    pub_type: 杂志文章


    authors: Logan KE,Chambers MA,Hewinson RG,Hogarth PJ

    更新日期:2005-12-01 00:00:00

  • Efficient extracellular production of recombinant Escherichia coli heat-labile enterotoxin B subunit by using the expression/secretion system of Bacillus brevis and its mucosal immunoadjuvanticity.

    abstract::A gene encoding the mature Escherichia coli heat-labile enterotoxin B subunit (LTB) was introduced in a vector pNU212 and expressed at high levels in Bacillus brevis HPD31. The maximum amount of recombinant LTB (rLTB) secreted into the modified 5PY medium containing erythromycin was about 350 mg l(-1) when cultivated ...


    pub_type: 杂志文章


    authors: Kozuka S,Yasuda Y,Isaka M,Masaki N,Taniguchi T,Matano K,Moriyama A,Ohkuma K,Goto N,Udaka S,Tochikubo K

    更新日期:2000-03-06 00:00:00

  • A phase I evaluation of inactivated influenza A/H5N1 vaccine administered by the intradermal or the intramuscular route.

    abstract::In a phase I clinical trial, one hundred healthy young adults were randomized to receive two doses 28 days apart of an inactivated, subvirion vaccine containing 15 or 45microg of influenza A/H5N1 hemagglutinin (HA) by the intramuscular (IM) route, or 3 or 9microg of H5 HA by the intradermal(ID) route. Seventy-seven su...


    pub_type: 杂志文章,随机对照试验


    authors: Patel SM,Atmar RL,El Sahly HM,Cate TR,Keitel WA

    更新日期:2010-04-09 00:00:00

  • Transepithelial immunomodulation by cholera toxin and non-toxic derivatives.

    abstract::Comparative analyses of murine dendritic cells (DC) isolated from the skin and from the intestinal mucosa after exposure to cholera toxin and its non-toxic B subunit disclose striking differences regarding the migratory and functional behaviour of these cells. The nature of the epithelial microenvironment, especially ...


    pub_type: 杂志文章


    authors: Anjuère F,Luci C,Hervouet C,Rousseau D,Czerkinsky C

    更新日期:2006-04-12 00:00:00

  • Pneumococcal vaccination in patients with systemic lupus erythematosus: A multicenter placebo-controlled randomized double-blind study.

    abstract:BACKGROUND:Invasive pneumococcal disease and respiratory tract infections are both frequent and severe in patients with systemic lupus erythematosus (SLE). This study aimed to compare the immunological efficacy and safety of pneumococcal vaccination with the 23-valent polysaccharide (PPS) vaccine alone to a sequential ...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Grabar S,Groh M,Bahuaud M,Le Guern V,Costedoat-Chalumeau N,Mathian A,Hanslik T,Guillevin L,Batteux F,Launay O,VACCILUP study group.

    更新日期:2017-09-05 00:00:00

  • Cost-effectiveness of childhood rotavirus vaccination in Taiwan.

    abstract:OBJECTIVE:Rotavirus is the most common cause of severe diarrhea in children. Two rotavirus vaccines (RotaTeq and Rotarix) have been licensed in Taiwan. We have investigated whether routine infant immunization with either vaccine could be cost-effective in Taiwan. METHODS:We modeled specific disease outcomes including ...


    pub_type: 杂志文章


    authors: Wu CL,Yang YC,Huang LM,Chen KT

    更新日期:2009-03-04 00:00:00

  • Effect of passive antibody on the immune response of cotton rats to purified F and G glycoproteins of respiratory syncytial virus (RSV).

    abstract::The effect of passively transferred RSV immune serum on the antibody response to a single dose of purified RSV fusion (F) and large (G) glycoproteins was studied in cotton rats. Passively transferred antibody that achieved serum antibody levels similar to those seen in newborn human infants resulted in a seven- to eig...


    pub_type: 杂志文章


    authors: Murphy BR,Prince GA,Collins PL,Hildreth SW,Paradiso PR

    更新日期:1991-03-01 00:00:00

  • Immune responses to intradermal and intramuscular inactivated influenza vaccine among older age group.

    abstract:BACKGROUD:Influenza viruses cause substantial morbidity, especially in older age groups. Thus, they are amongst high priority groups for routine vaccination. However, vaccine-induced immune responses and effectiveness were reported as relatively low. This study aims to systemically compare the immune responses elicited...


    pub_type: 临床试验,杂志文章


    authors: Boonnak K,Dhitavat J,Thantamnu N,Kosoltanapiwat N,Auayporn M,Jiang L,Puthavathana P,Pitisuttithum P

    更新日期:2017-12-19 00:00:00

  • A DNA vaccine encoding genetic fusions of carcinoembryonic antigen (CEA) and granulocyte/macrophage colony-stimulating factor (GM-CSF).

    abstract::The anti-tumor immunologic effects of plasmid DNA vaccines encoding human carcinoembryonic antigen (CEA) fused to mouse granulocyte/macrophage colony-stimulating factor (GM-CSF) were examined. Immunization of C57BL/6 mice with the CEA-GMCSF fusion plasmids in a three injection, high-dose immunization schedule led to T...


    pub_type: 杂志文章


    authors: Lima J,Jenkins C,Guerrero A,Triozzi PL,Shaw DR,Strong TV

    更新日期:2005-01-26 00:00:00

  • Vaccine effectiveness against severe laboratory-confirmed influenza in children: results of two consecutive seasons in Italy.

    abstract:OBJECTIVE:To evaluate the effectiveness of seasonal influenza vaccine in preventing Emergency Department (ED) visits and hospitalisations for influenza like illness (ILI) in children. METHODS:We conducted a test negative case-control study during the 2011-2012 and 2012-2013 influenza seasons. Eleven paediatric hospita...


    pub_type: 杂志文章


    authors: Menniti-Ippolito F,Da Cas R,Traversa G,Santuccio C,Felicetti P,Tartaglia L,Trotta F,Di Pietro P,Barabino P,Renna S,Riceputi L,Tovo PA,Gabiano C,Urbino A,Baroero L,Le Serre D,Virano S,Perilongo G,Daverio M,Gnoato E,

    更新日期:2014-07-31 00:00:00

  • Application of the Geographic Information System (GIS) in immunisation service delivery; its use in the 2017/2018 measles vaccination campaign in Nigeria.

    abstract:BACKGROUND:As global effort is made towards measles elimination, the use of innovative technology to enhance planning for the campaign has become critical. GIS technology has been applied to track polio vaccination activities in Nigeria with encouraging outcomes. Despite numerous measles vaccination campaigns after the...


    pub_type: 杂志文章


    authors: Oteri J,Idi Hussaini M,Bawa S,Ibizugbe S,Lambo K,Mogekwu F,Wiwa O,Seaman V,Kolbe-Booysen O,Braka F,Nsubuga P,Shuaib F

    更新日期:2021-01-18 00:00:00

  • Tetanus and diphtheria antibodies and response to a booster dose in Brazilian HIV-1-infected women.

    abstract::Tetanus and diphtheria (Td) antibodies were studied in HIV-1-infected women during puerperium. HIV group (n=61) was compared with Control group (n=101). Twenty-one women from HIV and 13 from Control group who had antibody levels lower than 0.1 IU/mL received a booster with Td vaccine. Antibodies were assessed by doubl...


    pub_type: 杂志文章


    authors: Bonetti TC,Succi RC,Weckx LY,Tavares-Lopes L,de Moraes-Pinto MI

    更新日期:2004-09-09 00:00:00

  • WHO Working Group on revision of the Manual of Laboratory Methods for Testing DTP Vaccines-Report of two meetings held on 20-21 July 2006 and 28-30 March 2007, Geneva, Switzerland.

    abstract::This report reflects the discussion and conclusions of a WHO group of experts from National Regulatory Authorities (NRAs), National Control Laboratories (NCLs), vaccine industries and other relevant institutions involved in standardization and control of diphtheria, tetanus and pertussis vaccines (DTP), held on 20-21 ...




    authors: Corbel MJ,Das RG,Lei D,Xing DK,Horiuchi Y,Dobbelaer R,WHO Working Group.

    更新日期:2008-04-07 00:00:00

  • Effect of thiomersal on dissociation of intact (146S) foot-and-mouth disease virions into 12S particles as assessed by novel ELISAs specific for either 146S or 12S particles.

    abstract::Intact (146S) foot-and-mouth disease virions (FMDVs) can dissociate into specific (12S) viral capsid degradation products. Using two single-domain antibody fragments that bind specifically to either 146S or 12S particles we developed two ELISAs for the quantification of these particles in FMDV antigen preparations use...


    pub_type: 杂志文章


    authors: Harmsen MM,Fijten HP,Westra DF,Coco-Martin JM

    更新日期:2011-03-24 00:00:00

  • Species-specific CD1-restricted innate immunity for the development of HIV vaccine.

    abstract::The human immune defense system is composed of two distinct elements: innate immunity located primarily at body surfaces restricted by species-specific CD1 molecules and acquired immunity found mainly in internal compartments associated with individually restricted MHC molecules. Historically, effective vaccines have ...


    pub_type: 杂志文章,评审


    authors: Takahashi H

    更新日期:2010-05-26 00:00:00