Immunization of rabbits with Glossina pallidipes tsetse fly midgut proteins: effects on the fly and trypanosome transmission.


:Proteins isolated from the midgut of Glossina pallidipes were used to immunize rabbits and their efficacy as vaccine candidate(s) against the fly, and their potential to block transmission of Trypanosoma brucei rhodesiense assessed. Two fractions, detergent (DET) and aqueous (AQ) fractions were separated using a non-ionic detergent (Triton X-114) and a series of bioassay experiments carried out using serum obtained from rabbits immunized with either of the two fractions. The mortality rates of tsetse flies fed on serum from rabbits immunized with DET and AQ was 56 and 35%, respectively, as compared to 20% mortality in controls. The DET antigen(s) caused considerably higher mortality (chi(2)=1.194, P<0.05) than that on controls. These findings suggest that midgut proteins contain antigens that are lethal to tsetse flies, and are potential candidates for the development of anti-tsetse vaccine. When flies fed on serum derived from DET immunized rabbits were fed on T. b. rhodesiense infected blood, only 20% of them picked the infection. Very few flies (20%) fed on serum derived from DET immunized rabbits had infection of T. b. rhodesiense. In the control flies 45% of them had infection in the midgut with a higher and actively motile parasite load. Assessment of fecundity indicated significantly higher (chi(2)=2.117, P<0.05) larviposition for the control flies when compared to the AQ group of flies (chi(2)=1.054, P<0.05). Significant differences in abortions and pupal weights were also observed. These results suggest that midgut proteins contain antigens with potential for use in development of vaccine to block transmission of trypanosomes through tsetse.






Kinyua JK,Nguu EK,Mulaa F,Ndung'u JM




Has Abstract


2005-05-31 00:00:00
















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    pub_type: 杂志文章


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  • Well-defined and potent liposomal meningococcal B vaccines adjuvated with LPS derivatives.

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    pub_type: 杂志文章


    authors: Arigita C,Luijkx T,Jiskoot W,Poelen M,Hennink WE,Crommelin DJ,Ley Pv,Els Cv,Kersten GF

    更新日期:2005-10-17 00:00:00

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    pub_type: 杂志文章,随机对照试验


    authors: Bonifachich E,Chort M,Astigarraga A,Diaz N,Brunet B,Pezzotto SM,Bottasso O

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    更新日期:1992-01-01 00:00:00

  • Potentiation of an anthrax DNA vaccine with electroporation.

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    pub_type: 临床试验,杂志文章


    authors: Rendi-Wagner P,Kundi M,Zent O,Banzhoff A,Jaehnig P,Stemberger R,Dvorak G,Grumbeck E,Laaber B,Kollaritsch H

    更新日期:2004-12-09 00:00:00

  • Multiple CD4 and CD8 T-cell activation parameters predict vaccine efficacy in vivo mediated by individual DC-activating agonists.

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    authors: Welters MJ,Bijker MS,van den Eeden SJ,Franken KL,Melief CJ,Offringa R,van der Burg SH

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    pub_type: 杂志文章,评审


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  • Genetic stability of the live attenuated Bordetella pertussis vaccine candidate BPZE1.

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    authors: DeVelasco EA,Dekker HA,Antal P,Jalink KP,van Strijp JA,Verheul AF,Verhoef J,Snippe H

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    authors: Pallikkuth S,Bolivar H,Fletcher MA,Babic DZ,De Armas LR,Gupta S,Termini JM,Arheart KL,Stevenson M,Tung FY,Fischl MA,Pahwa S,Stone GW

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