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Hypothermia of 8 degrees C protects cultured retinal pigment epithelial cells and retinal ganglion cells against trypan blue toxicity.

Abstract：

PURPOSE:To determine whether hypothermia of 8 degrees C can protect cultured human retinal pigment epithelial (ARPE-19) cells and rat retinal ganglion cells (RGC-5) against trypan blue (TB) toxicity. DESIGN:Laboratory investigation. METHODS:ARPE-19 cells and RGC-5 were exposed to balanced salt solution as controls, and 0.05% and 0.5% TB at 37 degrees C, and at 8 degrees C for one minute. The percentage of surviving cells was determined by the resazurin test. RESULTS:TB induced a statistically significant decrease in the percentage of ARPE-19 cells surviving at 0.5% TB at 37 degrees C (P < .01). Conversely, TB induced a statistically significant decrease in the percentage of RGC-5 surviving at all conditions except for 0.05% TB at 8 degrees C (0.05% 37 degrees C; P < .05, 0.5% 37 degrees C and 8 degrees C; P < .01). CONCLUSIONS:These results indicate that reducing the temperature to 8 degrees C has a protective effect against the TB toxicity for ARPE-19 cells and RGC-5 in culture.

journal_name

Am J Ophthalmol

journal_title

American journal of ophthalmology

authors

Kunikata H,Abe T,Murata H,Sagara Y,Wakusawa R,Sato H,Yoshida M,Fuse N,Tamai M

doi

10.1016/j.ajo.2005.10.054

subject

Has Abstract

pub_date

2006-04-01 00:00:00

pages

754-6

issue

4

eissn

0002-9394

issn

1879-1891

pii

S0002-9394(05)01175-X

journal_volume

141

pub_type

杂志文章

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