Abstract:
:The rpoB gene encoding the beta subunit of the DNA-dependent RNA polymerase was molecularly characterized by PCR amplification and DNA sequencing in 26 Brucella reference strains by using primers selected according to the B. melitensis 16 M rpoB published sequence. Comparison of the rpoB nucleotide sequence of all Brucella strains analysed revealed specific nucleotide variations associated with different Brucella species and biovars. 17 rpoB alleles were recognized and new Brucella typing is proposed. Our results suggest that the rpoB gene polymorphism can be used to identify all Brucella species and most of the biovars, offering an improvement over conventional typing methods.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Marianelli C,Ciuchini F,Tarantino M,Pasquali P,Adone Rdoi
10.1016/j.micinf.2005.10.008subject
Has Abstractpub_date
2006-03-01 00:00:00pages
860-5issue
3eissn
1286-4579issn
1769-714Xpii
S1286-4579(05)00376-Xjournal_volume
8pub_type
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