Immunogenicity and structural characterisation of an in vitro folded meningococcal siderophore receptor (FrpB, FetA).

Abstract:

:The iron-limitation-inducible protein FrpB of Neisseria meningitidis is an outer-membrane-localized siderophore receptor. Because of its abundance and its capacity to elicit bactericidal antibodies, it is considered a vaccine candidate. Bactericidal antibodies against FrpB are, however, type-specific. Hence, an FrpB-based vaccine should comprise several FrpB variants to be capable of providing broad protection. To facilitate the development of a meningococcal subunit vaccine, we have established a procedure to obtain large quantities of the protein in a native-like conformation. The protein was expressed without its signal sequence in Escherichia coli, where it accumulated in inclusion bodies. After in vitro folding, the protein was biochemically, biophysically and biologically characterised. Our results show that in vitro folded FrpB assembles into oligomers, presumably dimers, and that it induces high levels of bactericidal antibodies in laboratory animals.

journal_name

Microbes Infect

journal_title

Microbes and infection

authors

Kortekaas J,Müller SA,Ringler P,Gregorini M,Weynants VE,Rutten L,Bos MP,Tommassen J

doi

10.1016/j.micinf.2006.04.011

subject

Has Abstract

pub_date

2006-07-01 00:00:00

pages

2145-53

issue

8

eissn

1286-4579

issn

1769-714X

pii

S1286-4579(06)00174-2

journal_volume

8

pub_type

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