Abstract:
:The JAK2V617F mutation is present in most patients with polycythemia vera (PV) and in some patients with essential thrombocythemia (ET) and myeloid metaplasia/myelofibrosis (MMM). We sought to investigate the relationship between granulocyte clonality and JAK2V617F allelic ratio. A total of 168 of 190 female patients were informative for a clonality assay at the HUMARA locus; 80% of MMM, 75% of PV, and 67% of ET patients demonstrated clonal granulopoiesis. The JAK2V617F allele was detected by quantitative real-time polymerase chain reaction (PCR) in 99% of PV, 72% of ET, and 39% of MMM. A correlation between clonality and JAK2V617F allelic ratio was demonstrated for PV (P < .001) but not for ET or MMM (both P > .6). These data suggest that acquisition of the JAK2V617F mutation may be sufficient for the development of PV, but additional genetic events are necessary in ET and MMM. In addition, some ET and MMM patients with clonal granulopoiesis have somatic mutations other than JAK2V617F.
journal_name
Bloodjournal_title
Bloodauthors
Levine RL,Belisle C,Wadleigh M,Zahrieh D,Lee S,Chagnon P,Gilliland DG,Busque Ldoi
10.1182/blood-2005-09-3900subject
Has Abstractpub_date
2006-05-15 00:00:00pages
4139-41issue
10eissn
0006-4971issn
1528-0020pii
2005-09-3900journal_volume
107pub_type
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