Abstract:
:In spontaneous fibrinolysis of an alpha 2-plasmin inhibitor-deficient plasma clot or tissue-type plasminogen activator-induced fibrinolysis in a purified system without alpha 2-plasmin inhibitor, the lysis was faster when factor XIII-mediated crosslinking of fibrin to fibrin did not occur. During the initial period, the binding of plasminogen to fibrin steadily increased with incubation time. The initial level and subsequent increase of the binding, which may be critical for the subsequent development of fibrinolysis, were more remarkable when fibrin was not crosslinked. The amount of glu- or lys-plasminogen bound to noncrosslinked fibrin was around 4 or 1.5 times larger than the amount of the respective plasminogen bound to crosslinked fibrin. Plasmin was also found to be bound to noncrosslinked fibrin twice as much as the amount bound to crosslinked fibrin. Structural changes induced by crosslinking of fibrin alpha-chain may reduce either the affinity or the number of available complementary sites to lysine binding sites of plasmin(ogen), thereby decreasing the binding of plasmin(ogen) to fibrin. These results suggest that an increased affinity of noncrosslinked fibrin for plasmin(ogen) is contributory to the accelerated fibrinolysis observed in factor XIII deficiency, in addition to an absence of crosslinking of alpha 2-plasmin inhibitor to fibrin.
journal_name
Bloodjournal_title
Bloodauthors
Sakata Y,Mimuro J,Aoki Nsubject
Has Abstractpub_date
1984-06-01 00:00:00pages
1393-401issue
6eissn
0006-4971issn
1528-0020journal_volume
63pub_type
杂志文章相关文献
BLOOD文献大全abstract::Fanconi anemia (FA) is a hereditary disorder caused by mutations in any 1 of 22 FA genes. The disease is characterized by hypersensitivity to interstrand crosslink (ICL) inducers such as mitomycin C (MMC). In addition to promoting ICL repair, FA proteins such as RAD51, BRCA2, or FANCD2 protect stalled replication fork...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019003782
更新日期:2020-07-31 00:00:00
abstract::Although thrombin is a key enzyme in the coagulation cascade and is required for both normal hemostasis and pathologic thrombogenesis, it also participates in its own negative feedback via activation of protein C, which downregulates thrombin generation by enzymatically inactivating factors Va and VIIIa. Our group and...
journal_title:Blood
pub_type: 杂志文章,随机对照试验
doi:10.1182/blood.2019002771
更新日期:2020-02-27 00:00:00
abstract::Combined deficiency of ankyrin and spectrin represents the most common biochemical abnormality in hereditary spherocytosis (HS). To examine whether a decrease in ankyrin mRNA represents a frequent cause of this type of HS, we took advantage of the reported (AC)n microsatellite polymorphism in the 3' untranslated regio...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-06-01 00:00:00
abstract::Fetal hemoglobin (HbF, α2γ2) induction is a well-validated strategy for sickle cell disease (SCD) treatment. Using a small-molecule screen, we found that UNC0638, a selective inhibitor of EHMT1 and EHMT2 histone methyltransferases, induces γ-globin expression. EHMT1/2 catalyze mono- and dimethylation of lysine 9 on hi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-06-649087
更新日期:2015-10-15 00:00:00
abstract::The presence of circulating tumor idiotype interferes with the in vivo effectiveness of anti-idiotype antibodies. We developed two assays that permit identification of patients with high levels of serum idiotype without the need for first producing an anti-idiotype antibody. A cell suspension made from the tumor was c...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1987-04-01 00:00:00
abstract::Cyclin dependent kinase (CDK) inhibitors, such as flavopiridol, demonstrate significant single-agent activity in chronic lymphocytic leukemia (CLL), but the mechanism of action in these nonproliferating cells is unclear. Here we demonstrate that CLL cells undergo autophagy after treatment with therapeutic agents, incl...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-12-400184
更新日期:2012-08-09 00:00:00
abstract::We have identified an intergenic transcriptional activity that is located between the human HOXA1 and HOXA2 genes, shows myeloid-specific expression, and is up-regulated during granulocytic differentiation. The novel gene, termed HOTAIRM1 (HOX antisense intergenic RNA myeloid 1), is transcribed antisense to the HOXA g...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-06-162164
更新日期:2009-03-12 00:00:00
abstract::Soluble HLA-G (sHLA-G) inhibits natural killer (NK) cell functions. Here, we investigated sHLA-G-mediated modulation of (1) chemokine receptor and NK receptor expression and function and (2) cytokine and chemokine secretion in CD56bright and CD56dim NK cells. sHLA-G-treated or untreated peripheral blood (PB) and tonsi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-05-352393
更新日期:2011-11-24 00:00:00
abstract::It has recently been shown that the ultralarge platelet-recruiting von Willebrand factor (VWF) strings formed immediately at exocytosis from endothelial cells may be anchored to the cell surface by interaction with the integral membrane protein P-selectin. This finding of a new binding partner for VWF immediately prom...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-09-3635
更新日期:2006-05-15 00:00:00
abstract::Interactions between vascular endothelial cells and blood platelets have been investigated using a model microcirculation consisting of microcarrier beads colonized with human umbilical vein endothelial cells (HUVECs) and perfused with washed platelet suspensions. To simulate the effects of endothelial desquamation an...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1986-06-01 00:00:00
abstract::We retrospectively analyzed outcomes among 567 patients with hematologic malignancies who had hematopoietic cell transplantation from human leukocyte antigen-identical sibling donors between 2001 and 2007 for a correlation between statin use and risk of graft-versus-host disease (GVHD). Compared with allografts where ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-08-240358
更新日期:2010-02-11 00:00:00
abstract::Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous disease defined by transcriptional classifications, specific signaling and survival pathways, and multiple low-frequency genetic alterations. Preclinical model systems that capture the genetic and functional heterogeneity of DLBCL are urgently needed. Here, we g...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-09-672352
更新日期:2016-05-05 00:00:00
abstract::The presence of multiple VHDJH joinings in upwards of 30% of acute lymphoblastic leukemias (ALL) suggests a relative instability of the rearranged immunoglobulin heavy chain (IgH) gene, but the mechanisms involved are not completely understood. An investigation of the structure of the VHDJH joinings using complementar...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::Recent studies on the immunoglobulin variable heavy chain (IgV(H)) genes have revealed that B-cell chronic lymphocytic leukemia (B-CLL) consists of at least 2 clinical entities with either somatically mutated or unmutated V(H) genes. We have analyzed the V(H) gene mutation status and V(H) gene usage in 119 B-CLL cases...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v99.6.2262
更新日期:2002-03-15 00:00:00
abstract::Surrogate light chain expression during B lineage differentiation was examined by using indicator fluorochrome-filled liposomes in an enhanced immunofluorescence assay. Pro-B cells bearing surrogate light chain components were found in mice, but not in humans. A limited subpopulation of relatively large pre-B cells in...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v99.7.2459
更新日期:2002-04-01 00:00:00
abstract::Escherichia coli were labeled with 59Fe and then either treated with myeloperoxidase, H2O2, and chloride or opsonized and mixed with human neutrophils. The myeloperoxidase system at pH 7.4 caused release of most of the bacterial 59Fe. A similar result has been obtained by Rosen and Klebanoff (J Biol Chem 257:13731, 19...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-02-15 00:00:00
abstract::The human peripheral B-cell compartment displays a large population of immunoglobulin M-positive, immunoglobulin D-positive CD27(+) (IgM(+)IgD(+)CD27(+)) "memory" B cells carrying a mutated immunoglobulin receptor. By means of phenotypic analysis, complementarity-determining region 3 (CDR3) spectratyping during a T-in...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-01-0346
更新日期:2004-12-01 00:00:00
abstract::The endothelial cell protein C receptor (EPCR) augments protein C activation by the thrombin-thrombomodulin complex. Deletion of the EPCR gene (Procr) in mice leads to embryonic lethality before embryonic day 10 (E10.0). EPCR is detected in the giant trophoblast cells at the feto-maternal boundary from E7.5 and weakly...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-01-0406
更新日期:2005-10-15 00:00:00
abstract::There have been several new developments in the field of autoimmune neutropenia over the past decade. Neutropenia caused by antibodies directed against granulocyte precursor cells, the oligoclonal nature of antineutrophil antibodies, and the expanding knowledge of neutrophil antigens, particularly in relationship to a...
journal_title:Blood
pub_type: 杂志文章,评审
doi:
更新日期:1993-04-15 00:00:00
abstract::The effect of recombinant human erythropoietin (rHuEpo) on megakaryopoiesis remains controversial. Treatment with rHuEpo in renal failure patients has been associated with a slight elevation of platelet counts. In animal studies, high doses of rHuEpo produced an increase of platelet counts followed by a gradual return...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-05-15 00:00:00
abstract::Long-living memory stem T cells (T(SCM)) with the ability to self-renew and the plasticity to differentiate into potent effectors could be valuable weapons in adoptive T-cell therapy against cancer. Nonetheless, procedures to specifically target this T-cell population remain elusive. Here, we show that it is possible ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-05-431718
更新日期:2013-01-24 00:00:00
abstract::The prevalence, the prognostic effect, and interaction with other molecular markers of DNMT3A mutations was studied in 415 patients with acute myeloid leukemia (AML) younger than 60 years. We show mutations in DNMT3A in 96 of 415 patients with newly diagnosed AML (23.1%). Univariate Cox regression analysis showed that...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-07-367961
更新日期:2012-06-14 00:00:00
abstract::Recent population-based studies demonstrate an increased fracture risk with monoclonal gammopathy of undetermined significance (MGUS). The etiology of this increased risk remains unclear, however, because areal bone mineral density (aBMD) measurements by dual-energy x-ray absorptiometry cannot assess bone microstructu...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-04-351437
更新日期:2011-12-15 00:00:00
abstract::The mechanical properties of sickle erythrocyte membranes were evaluated in the ektacytometer. When ghosts from the total red blood cell population were examined, the rigidity of the resealed ghosts and their rate of fragmentation by shear stress (t1/2) were normal. However, fractionation on Stractan density gradients...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-04-15 00:00:00
abstract::The prognosis of acute myeloid leukemia (AML) is very poor in elderly patients, especially in those classically defined as having unfavorable cytogenetics. The recent monosomal karyotype (MK) entity, defined as 2 or more autosomal monosomies or combination of 1 monosomy with structural abnormalities, has been reported...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-09-307264
更新日期:2011-07-21 00:00:00
abstract::Induction of red blood cell (RBC) fetal hemoglobin (HbF; α2γ2) ameliorates the pathophysiology of sickle cell disease (SCD) by reducing the concentration of sickle hemoglobin (HbS; α2βS2) to inhibit its polymerization. Hydroxyurea (HU), the only US Food and Drug Administration (FDA)-approved drug for SCD, acts in part...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2017-11-814335
更新日期:2018-07-19 00:00:00
abstract::Graft-versus-host disease (GVHD) is the main complication of allogeneic stem cell transplantation. However, diagnosis of GVHD and evaluation of response to immunosuppressive treatment is sometimes difficult. Since apoptosis is the histopathologic hallmark in GVHD, we investigated whether active GVHD-induced target org...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-10-049817
更新日期:2007-12-15 00:00:00
abstract::We describe the cloning and characterization of Siglec-H, a novel murine CD33-related siglec-like molecule with 2 immunoglobulin domains. Unlike other CD33-related siglecs, Siglec-H lacks tyrosine-based signaling motifs in its cytoplasmic tail. Although Siglec-H has the typical structural features required for sialic ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-09-3842
更新日期:2006-05-01 00:00:00
abstract::Attempts to maintain or expand primitive hematopoietic stem cells in vitro without the concomitant loss of their differentiative and proliferative potential in vivo have largely been unsuccessful. To investigate this problem, we compared the ability of three cloned bone marrow (BM) stromal cell lines to support the gr...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
abstract::To investigate the role of peritoneal mesothelial cells in regulating hematopoiesis, as well as inflammation, healing, and tissue regeneration processes, long-term cultures of peritoneal mesothelial cells from human endocavitarian fluids were established. The purity of the cell population was assessed by morphologic a...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00