Fish soluble Toll-like receptor (TLR)5 amplifies human TLR5 response via physical binding to flagellin.


:Fish has a soluble form of TLR5 ortholog (TLR5S), which does not exist in mammals. We identified TLR5S from rainbow trout and named rtTLR5S, which was about 38% homologous to the extracellular domains of human (hu) and mouse TLR5. Adjuvancy of rtTLR5S to flagellin response by human TLR5 (huTLR5) was tested in this study. A chimera constructed of rtTLR5S and the intracellular TIR of huTLR5 expressed on HeLa cells signaled the presence of various species of bacterial flagellin resulting in NK-kappaB activation. huTLR5S, when co-expressed with rtTLR5S in HeLa cells, augmented response to flagellin resulting in robust huTLR5-mediated NF-kappaB activation. Physical binding of flagellin to rtTLR5S was detected under the conditions where huTLR5 induced rtTLR5S-amplified NF-kappaB activation. Signal amplification by rtTLR5S was specific to huTLR5: no other huTLRs tested were responded to rtTLR5S. These results suggest that the soluble TLR5 serves as an adjuvant augmenting flagellin-TLR5-mediated NF-kappaB activation even in human.






Tsujita T,Ishii A,Tsukada H,Matsumoto M,Che FS,Seya T




Has Abstract


2006-03-15 00:00:00
















  • Immunologic responses of bison to vaccination with Brucella abortus strain RB51: comparison of parenteral to ballistic delivery via compressed pellets or photopolymerized hydrogels.

    abstract::This study compared responses of bison calves to 10(10)CFU of Brucella abortus strain RB51 (SRB51) delivered by parenteral or ballistic methods. Two types of biobullet payloads were evaluated; compacted SRB51 pellets or SRB51 encapsulated in photopolymerized poly(ethylene glycol) hydrogels. Bison were vaccinated with ...


    pub_type: 杂志文章


    authors: Olsen SC,Christie RJ,Grainger DW,Stoffregen WS

    更新日期:2006-02-27 00:00:00

  • B cell immunopathology during HIV-1 infection: lessons to learn for HIV-1 vaccine design.

    abstract::Induction of broad HIV-1 neutralizing antibodies should be a major goal of an effective HIV-1 vaccine. However, B cells are severely damaged during HIV-1 infection with loss of memory B cells and decline of serological memory. The molecular events leading to B cell damage must be further characterized with the aim of ...


    pub_type: 杂志文章,评审


    authors: Cagigi A,Nilsson A,De Milito A,Chiodi F

    更新日期:2008-06-06 00:00:00

  • Construction and biological characterization of artificial recombinants between a wild type flavivirus (Kunjin) and a live chimeric flavivirus vaccine (ChimeriVax-JE).

    abstract::Although the theoretical concern of genetic recombination has been raised related to the use of live attenuated flavivirus vaccines [Seligman, Gould, Lancet 2004;363:2073-5], it has little foundation [e.g., Monath TP, Kanesa-Thasan N, Guirakhoo F, Pugachev K, Almond J, Lang J, et al. Vaccine 2005;23:2956-8]. To invest...


    pub_type: 杂志文章


    authors: Pugachev KV,Schwaiger J,Brown N,Zhang ZX,Catalan J,Mitchell FS,Ocran SW,Rumyantsev AA,Khromykh AA,Monath TP,Guirakhoo F

    更新日期:2007-09-17 00:00:00

  • Predictors for influenza vaccine acceptance among patients with inflammatory rheumatic diseases.

    abstract:BACKGROUND:Patients with inflammatory rheumatic diseases are at higher risk for influenza and current guidelines recommend vaccination for this group of patients. The aim of this study was to evaluate the vaccination coverage and predictors for influenza vaccination among patients with inflammatory rheumatic diseases. ...


    pub_type: 杂志文章


    authors: Harrison N,Poeppl W,Miksch M,Machold K,Kiener H,Aletaha D,Smolen JS,Forstner C,Burgmann H,Lagler H

    更新日期:2018-08-06 00:00:00

  • Would an in vitro ELISA test be a suitable alternative potency method to the in vivo immunogenicity assay commonly used in the context of international Hepatitis A vaccines batch release?

    abstract::Since many years Afssaps applies the 3R's strategy (replacement, reduction and refinement) for the use of laboratory animal testing in the framework of vaccine batch release. In this context, for Hepatitis A vaccines, a study was carried out to assess the feasibility of replacing the in vivo "Gold Standard" potency as...


    pub_type: 杂志文章


    authors: Poirier B,Variot P,Delourme P,Maurin J,Morgeaux S

    更新日期:2010-02-17 00:00:00

  • BCG vaccine may generate cross-reactive T cells against SARS-CoV-2: In silico analyses and a hypothesis.

    abstract::The world is facing the rising emergency of SARS-CoV-2. The outbreak of COVID-19 has caused a global public health and economic crisis.Recent epidemiological studies have shown that a possible association of BCG vaccination program with decreased COVID-19-related risks, suggesting that BCG may provide protection again...


    pub_type: 杂志文章


    authors: Tomita Y,Sato R,Ikeda T,Sakagami T

    更新日期:2020-09-22 00:00:00

  • A DNA vaccine encoding VP22 of herpes simplex virus type I (HSV-1) and OprF confers enhanced protection from Pseudomonas aeruginosa in mice.

    abstract::Pseudomonas aeruginosa antimicrobial resistance is a major therapeutic challenge. DNA vaccination is an attractive approach for antigen-specific immunotherapy against P. aeruginosa. We explored the feasibility of employing Herpes simplex virus type 1 tegument protein, VP22, as a molecular tool to enhance the immunogen...


    pub_type: 杂志文章


    authors: Yu X,Wang Y,Xia Y,Zhang L,Yang Q,Lei J

    更新日期:2016-08-17 00:00:00

  • Efficacy of parainfluenza virus 5 (PIV5)-based tuberculosis vaccines in mice.

    abstract::Mycobacterium tuberculosis, the etiological agent of tuberculosis (TB), is an important human pathogen. Bacillus Calmette-Guérin (BCG), a live, attenuated variant of Mycobacterium bovis, is currently the only available TB vaccine despite its low efficacy against the infectious pulmonary form of the disease in adults. ...


    pub_type: 杂志文章


    authors: Chen Z,Gupta T,Xu P,Phan S,Pickar A,Yau W,Karls RK,Quinn FD,Sakamoto K,He B

    更新日期:2015-12-16 00:00:00

  • Prothymosin alpha enhances protective immune responses induced by oral DNA vaccination against pseudorabies delivered by Salmonella choleraesuis.

    abstract::Previously, we showed that vaccination with the glycoprotein D (gD) gene of pseudorabies virus (PrV) delivered by Escherichia coli induced protective immune responses. In this study, we report that oral DNA vaccination with attenuated Salmonella choleraesuis carrying the PrV gD gene conferred protective immunity in mi...


    pub_type: 杂志文章


    authors: Shiau AL,Chen YL,Liao CY,Huang YS,Wu CL

    更新日期:2001-07-16 00:00:00

  • Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells.

    abstract::Cytomegalovirus infections are an important cause of disease for which no licensed vaccine exists. Recent studies have focused on the gH/gL/UL128-131 complex as antibodies to gH/gL/UL128-131 neutralize viral entry into epithelial cells. Prior studies have used cells from the retinal pigment epithelium, while to preven...


    pub_type: 杂志文章


    authors: Saccoccio FM,Sauer AL,Cui X,Armstrong AE,Habib el-SE,Johnson DC,Ryckman BJ,Klingelhutz AJ,Adler SP,McVoy MA

    更新日期:2011-03-24 00:00:00

  • Potential T cell epitopes within swine-origin triple reassortant influenza A (H3N2) variant virus which emerged in 2011: an immunoinformatics study.

    abstract::An immuno-informatics study was conducted to determine possible pre-existing T cellular immunity to the recently emerged swine-origin triple reassortant H3N2 variant (S-OtrH3N2v-2011) which acquired the matrix gene of influenza A (H1N1)pdm09. Given the genetic origin of S-OtrH3N2v-2011, our study focused on the hemagg...


    pub_type: 杂志文章


    authors: Duvvuri VR,Marchand-Austin A,Eshaghi A,Patel SN,Low DE,Gubbay JB

    更新日期:2012-09-14 00:00:00

  • Lower peripheral blood CD14+ monocyte frequency and higher CD34+ progenitor cell frequency are associated with HBV vaccine induced response in HIV infected individuals.

    abstract::We evaluated immunologic predictors of response to HBV vaccine administered in the presence or absence of GM-CSF in HIV infected individuals. We measured peripheral blood hematopoietic progenitor, monocyte and myeloid-derived suppressor cell (MDSC) frequencies, and expression of GMCSF receptor on monocytes and MDSCs, ...


    pub_type: 杂志文章,随机对照试验


    authors: Anthony DD,Umbleja T,Aberg JA,Kang M,Medvik K,Lederman MM,Peters MG,Koziel MJ,Overton ET

    更新日期:2011-04-27 00:00:00

  • Sudden infant death syndrome (SIDS): are common bacterial toxins responsible, and do they have a vaccine potential?

    abstract::Despite extensive research, no unifying concept has satisfactorily explained the cause of the sudden infant death syndrome (SIDS). The details are briefly outlined of some of the evidence supporting the hypothesis that common bacterial toxins are important in the aetiology of SIDS. These bacterial toxins act as trigge...


    pub_type: 杂志文章,评审


    authors: Murrell TG,Murrell WG,Lindsay JA

    更新日期:1994-03-01 00:00:00

  • Comparison of influenza virus yields and apoptosis-induction in an adherent and a suspension MDCK cell line.

    abstract::Cell culture-based manufacturing of influenza vaccines is ideally based on easily scalable platforms using suspension cells that grow in chemically defined media. Consequently, different adherent cell lines selected for high virus yields were adapted to grow in suspension culture. This includes the MDCK suspension cel...


    pub_type: 杂志文章


    authors: Peschel B,Frentzel S,Laske T,Genzel Y,Reichl U

    更新日期:2013-11-19 00:00:00

  • European citizens' opinions on immunisation.

    abstract::As part of a larger study exploring how European citizens' balance issues of public and private interest and the extent to which they are prepared to accept State intervention on a range of public health issues, focus group participants were asked whether childhood immunisation should be a matter of parental choice or...


    pub_type: 杂志文章


    authors: Moran N,Shickle D,Richardson E

    更新日期:2008-01-17 00:00:00

  • A single dose of an ISCOM influenza vaccine induces long-lasting protective immunity against homologous challenge infection but fails to protect Cynomolgus macaques against distant drift variants of influenza A (H3N2) viruses.

    abstract::Since the production of influenza vaccines is complicated by the continuous variation of these viruses, it would be desirable to develop vaccines that induce cross-protective immunity against influenza virus strains that circulate in subsequent winter epidemics. We have recently demonstrated that antibodies induced af...


    pub_type: 杂志文章


    authors: Rimmelzwaan GF,Baars M,van Amerongen G,van Beek R,Osterhaus AD

    更新日期:2001-10-12 00:00:00

  • Development of a recombinant Leishmania major strain sensitive to ganciclovir and 5-fluorocytosine for use as a live vaccine challenge in clinical trials.

    abstract::To provide a safer live challenge strain for use in clinical vaccine trials, a double drug sensitive strain of Leishmania major was derived using advances in gene targeting technology by stably introducing into the chromosome a modified HSV-1 thymidine kinase gene (tk), conferring increased sensitivity to ganciclovir ...


    pub_type: 杂志文章


    authors: Davoudi N,Tate CA,Warburton C,Murray A,Mahboudi F,McMaster WR

    更新日期:2005-01-19 00:00:00

  • Seasonal influenza vaccination policies in the Eastern Mediterranean Region: Current status and the way forward.

    abstract:BACKGROUND:The World Health Organization recommends annual influenza vaccination, especially in high-risk groups. Little is known about the adoption and implementation of influenza vaccination policies in the Eastern Mediterranean Region. METHODS:A survey was distributed to country representatives at the ministries of...


    pub_type: 历史文章,杂志文章


    authors: Abubakar A,Melhem N,Malik M,Dbaibo G,Khan WM,Zaraket H

    更新日期:2019-03-14 00:00:00

  • Safety and immunogenicity of unadjuvanted subvirion monovalent inactivated influenza H3N2 variant (H3N2v) vaccine in children and adolescents.

    abstract:OBJECTIVE:In response to the emergence of influenza viruses with pandemic potential, we evaluated a swine-origin influenza A/H3N2 variant (H3N2v) vaccine in children. STUDY DESIGN:This multicenter phase II open-label study assessed the safety and immunogenicity of two doses, 21 days apart, of investigational unadjuvan...


    pub_type: 杂志文章


    authors: Munoz FM,Anderson EJ,Bernstein DI,Harrison CJ,Pahud B,Anderson E,Creech CB,Berry AA,Kotloff KL,Walter EB,Atmar RL,Bellamy AR,Chang S,Keitel WA

    更新日期:2019-08-23 00:00:00

  • Can the vaccine adverse event reporting system be used to increase vaccine acceptance and trust?

    abstract::Vaccine refusal has an impact on public health, and the human pappillomavirus (HPV) vaccine is particularly underutilized. Research suggests that it may be difficult to change vaccine-related attitudes, and there is currently no good evidence to recommend any particular intervention strategy. One reason for vaccine he...


    pub_type: 杂志文章


    authors: Scherer LD,Shaffer VA,Patel N,Zikmund-Fisher BJ

    更新日期:2016-05-05 00:00:00

  • Influenza vaccination of future healthcare workers: a cross-sectional study of uptake, knowledge and attitudes.

    abstract::Promotional campaigns recommend immunisation against influenza in healthcare workers (HCWs) but the uptake in this group remains low. We conducted a survey study during the 2008-2009 influenza vaccination period amongst future HCWs to quantify uptake and identify barriers to immunisation. Overall uptake was 8.0% (95% ...


    pub_type: 杂志文章


    authors: Blank DL,Bodansky DM,Forbes A,Garde E,Story F,Roalfe AK,Tait L

    更新日期:2010-06-23 00:00:00

  • Challenges for the registration of vaccines in emerging countries: Differences in dossier requirements, application and evaluation processes.

    abstract::The divergence of regulatory requirements and processes in developing and emerging countries contributes to hamper vaccines' registration, and therefore delay access to high-quality, safe and efficacious vaccines for their respective populations. This report focuses on providing insights on the heterogeneity of regist...




    authors: Dellepiane N,Pagliusi S,Registration Experts Working Group.

    更新日期:2018-06-07 00:00:00

  • Pharmacist role in vaccination: Evidence and challenges.

    abstract::Vaccines prevent an estimated 2.5 million deaths worldwide each year and are amongst the most cost-effective preventive measures against infectious diseases. Despite the effectiveness and availability of vaccines in many parts of the world, vaccination rates and service uptake remains suboptimal among both healthcare ...


    pub_type: 杂志文章,评审


    authors: Poudel A,Lau ETL,Deldot M,Campbell C,Waite NM,Nissen LM

    更新日期:2019-09-20 00:00:00

  • Epidemiological patterns of hepatitis A in different parts of the world.

    abstract::Serological surveys in many communities show a high prevalence of antibodies to hepatitis A virus (HAV) in people over the age of 50 years. However, few of that age can recall a previous episode of hepatitis, indicating that subclinical infections are common. The outcome of infection with HAV depends on the age at whi...


    pub_type: 杂志文章,评审


    authors: Gust ID

    更新日期:1992-01-01 00:00:00

  • Safety and immunogenicity of influenza A(H5N1) vaccine stored up to twelve years in the National Pre-Pandemic Influenza Vaccine Stockpile (NPIVS).

    abstract:BACKGROUND:As part of the U.S. Department of Health and Human Services (HHS) Pandemic Influenza Plan preparedness and response strategy, the National Pre-Pandemic Influenza Vaccine Stockpile (NPIVS) program was established by the Biomedical Advanced Research and Development Authority (BARDA) in 2005 with the goal of bu...


    pub_type: 杂志文章,随机对照试验


    authors: Oshansky CM,Zhou J,Gao Y,Schweinle JE,Biscardi K,DeBeauchamp J,Pavetto C,Wollish A,BRITE Study Coordination Team.,Webby RJ,Cioce V,Donis RO,Bright RA

    更新日期:2019-01-14 00:00:00

  • Adjuvant activity of fish type I interferon shown in a virus DNA vaccination model.

    abstract::There is a need for more efficient vaccines to combat viral diseases of Atlantic salmon and other farmed fish. DNA vaccines are highly effective against salmonid rhabdoviruses, but have shown less effect against other viruses. In the present work we have studied if type I IFNs might be used as adjuvants in fish DNA va...


    pub_type: 杂志文章


    authors: Chang CJ,Sun B,Robertsen B

    更新日期:2015-05-15 00:00:00

  • The Meningitis Vaccine Project.

    abstract::Epidemic meningococcal meningitis is an important public health problem in sub-Saharan Africa. Current control measures rely on reactive immunizations with polysaccharide (PS) vaccines that do not induce herd immunity and are of limited effectiveness in those under 2 years of age. Conversely, polysaccharide conjugate ...


    pub_type: 杂志文章


    authors: LaForce FM,Konde K,Viviani S,Préziosi MP

    更新日期:2007-09-03 00:00:00

  • It's not all about autism: The emerging landscape of anti-vaccination sentiment on Facebook.

    abstract:BACKGROUND:Due in part to declining vaccination rates, in 2018 over 20 states reported at least one case of measles, and over 40,000 cases were confirmed in Europe. Anti-vaccine posts on social media may be facilitating anti-vaccination behaviour. This study aimed to systematically characterize (1) individuals known to...


    pub_type: 杂志文章


    authors: Hoffman BL,Felter EM,Chu KH,Shensa A,Hermann C,Wolynn T,Williams D,Primack BA

    更新日期:2019-04-10 00:00:00

  • Simple differentiation method of mumps Hoshino vaccine strain from wild strains by reverse transcription loop-mediated isothermal amplification (RT-LAMP).

    abstract::Mumps virus is still circulating and annual mumps outbreaks occur with fluctuating magnitudes in Japan. Aseptic meningitis has been reported after vaccination and it would be of importance to determine whether this was related to the vaccination. The objective of this study was to develop a sensitive, specific and rap...


    pub_type: 杂志文章


    authors: Yoshida N,Fujino M,Ota Y,Notomi T,Nakayama T

    更新日期:2007-01-26 00:00:00

  • RNA-based adjuvant CV8102 enhances the immunogenicity of a licensed rabies vaccine in a first-in-human trial.

    abstract:BACKGROUND:We report the first-in-concept human trial of the safety, tolerability and immunogenicity when a novel TLR 7/8/RIG I agonist RNA-based adjuvant, CV8102, was administered alone or mixed with fractional doses of a licensed rabies vaccine (Rabipur®) as model antigen. METHODS:The primary objective was to assess...


    pub_type: 杂志文章


    authors: Doener F,Hong HS,Meyer I,Tadjalli-Mehr K,Daehling A,Heidenreich R,Koch SD,Fotin-Mleczek M,Gnad-Vogt U

    更新日期:2019-03-22 00:00:00