Subunit contributions to histone methyltransferase activities of fly and worm polycomb group complexes.

abstract：:Genomic instability, including the ability to undergo gene amplification, is a hallmark of neoplastic cells. Similar to normal cells, "nonpermissive" REF52 cells do not develop resistance to N-(phosphonacetyl)-L-aspartate (PALA), an inhibitor of the synthesis of pyrimidine nucleotides, through amplification of cad, th...

journal_title：Molecular and cellular biology

authors： Chernova OB,Chernov MV,Ishizaka Y,Agarwal ML,Stark GR

更新日期：1998-01-01 00:00:00

abstract：:Thrombin stimulation of platelets induces a transient increase in the specific activity of pp60c-src followed by a redistribution of pp60c-src to the Triton X-100-insoluble, cytoskeleton-rich fraction. Concomitant with the observed increase in pp60c-src activity was a rapid dephosphorylation of tyrosine 527 in 10 to 1...

journal_title：Molecular and cellular biology

authors： Clark EA,Brugge JS

更新日期：1993-03-01 00:00:00

abstract：:An enzyme was isolated from a eucaryotic, Chlorella-like green alga infected with the virus PBCV-1 which exhibits type II restriction endonuclease activity. The enzyme recognized the sequence GATC and cleaved DNA 5' to the G. Methylation of deoxyadenosine in the GATC sequence inhibited enzyme activity. In vitro the en...

journal_title：Molecular and cellular biology

authors： Xia YN,Burbank DE,Uher L,Rabussay D,Van Etten JL

更新日期：1986-05-01 00:00:00

abstract：:Transcriptional activators interact with multisubunit coactivators that modify chromatin structure or recruit the general transcriptional machinery to their target genes. Budding yeast cells respond to amino acid starvation by inducing an activator of amino acid biosynthetic genes, Gcn4p. We conducted a comprehensive ...

journal_title：Molecular and cellular biology

authors： Swanson MJ,Qiu H,Sumibcay L,Krueger A,Kim SJ,Natarajan K,Yoon S,Hinnebusch AG

更新日期：2003-04-01 00:00:00

abstract：:"Neutropenia" refers to deficient numbers of neutrophils, the most abundant type of white blood cell. Two main forms of inherited neutropenia are cyclic neutropenia, in which neutrophil counts oscillate with a 21-day frequency, and severe congenital neutropenia, in which static neutropenia may evolve at times into leu...

journal_title：Molecular and cellular biology

authors： Salipante SJ,Rojas ME,Korkmaz B,Duan Z,Wechsler J,Benson KF,Person RE,Grimes HL,Horwitz MS

更新日期：2009-08-01 00:00:00

abstract：:Nonsense-mediated RNA decay (NMD) is an mRNA surveillance mechanism which rapidly degrades select cytoplasmic mRNAs. We and others have shown that NMD is a dynamically regulated process inhibited by amino acid deprivation, hypoxia, and other cellular stresses commonly generated by the tumor microenvironment. This inhi...

journal_title：Molecular and cellular biology

authors： Wengrod J,Martin L,Wang D,Frischmeyer-Guerrerio P,Dietz HC,Gardner LB

更新日期：2013-06-01 00:00:00

abstract：:We have determined the complete nucleotide sequences of two chicken beta-tubulin genes, beta 1 and beta 2. These genes display an unusual pattern of segmental homology which indicates that they originally arose by gene duplication and have subsequently coevolved by a process that included localized gene conversion or ...

journal_title：Molecular and cellular biology

authors： Sullivan KF,Lau JT,Cleveland DW

更新日期：1985-09-01 00:00:00

abstract：:Negative elongation factor (NELF) is a human transcription factor complex that cooperates with DRB sensitivity-inducing factor (DSIF)/hSpt4-hSpt5 to repress elongation by RNA polymerase II (RNAPII). NELF activity is associated with five polypeptides, including NELF-A, a candidate gene product for Wolf-Hirschhorn syndr...

journal_title：Molecular and cellular biology

authors： Yamaguchi Y,Inukai N,Narita T,Wada T,Handa H

更新日期：2002-05-01 00:00:00

abstract：:Ubiquitin is a multifunctional 76-amino-acid protein which plays critical roles in many aspects of cellular metabolism. In Caenorhabditis elegans, the major source of ubiquitin RNA is the polyubiquitin locus, UbiA. UbiA is transcribed as a polycistronic mRNA which contains 11 tandem repeats of ubiquitin sequence and p...

journal_title：Molecular and cellular biology

authors： Graham RW,Jones D,Candido EP

更新日期：1989-01-01 00:00:00

abstract：:Loss of heterozygosity (LOH) is a common genetic alteration in tumors and often extends several megabases to encompass multiple genetic loci or even whole chromosome arms. Based on marker and karyotype analysis of tumor samples, a significant fraction of LOH events appears to arise from mitotic recombination between h...

journal_title：Molecular and cellular biology

authors： Stark JM,Jasin M

更新日期：2003-01-01 00:00:00

abstract：:The Polycomb group proteins are involved in maintenance of the silenced state of several developmentally regulated genes. These proteins form large aggregates with different subunit compositions. To explore the nature of these complexes and their function, we used the full-length Eed (embryonic ectoderm development) p...

journal_title：Molecular and cellular biology

authors： Denisenko O,Shnyreva M,Suzuki H,Bomsztyk K

更新日期：1998-10-01 00:00:00

abstract：:The phosphoinositide phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] regulates the activity of many actin-binding proteins and as such is an important modulator of cytoskeleton organization during cell migration, for example. In migrating cells actin remodeling is tightly regulated and localized; therefore, how the ...

journal_title：Molecular and cellular biology

authors： Kisseleva M,Feng Y,Ward M,Song C,Anderson RA,Longmore GD

更新日期：2005-05-01 00:00:00

abstract：:We have previously reported that antibodies to phosphotyrosine recognize the phosphorylated forms of platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) receptors (Zippel et al., Biochim. Biophys. Acta 881:54-61, 1986, and Sturani et al., Biochem. Biophys. Res. Commun. 137:343-350, 1986). In this r...

journal_title：Molecular and cellular biology

authors： Sturani E,Zippel R,Toschi L,Morello L,Comoglio PM,Alberghina L

更新日期：1988-03-01 00:00:00

abstract：:Amino acids 106 to 143 and 354 to 433 of the human c-myc protein (439 amino acids) were shown to be required for the protein to suppress c-myc gene transcription and were found to exactly overlap with those necessary for c-myc to cooperate with ras oncogenes in the transformation of rat embryo fibroblasts. The essenti...

journal_title：Molecular and cellular biology

authors： Penn LJ,Brooks MW,Laufer EM,Littlewood TD,Morgenstern JP,Evan GI,Lee WM,Land H

更新日期：1990-09-01 00:00:00

abstract：:p27(Kip1) is a member of the Cip-Kip family of cyclin-dependent kinase (Cdk) inhibitors that binds to cyclin-Cdk complexes and inhibits their catalytic activity in response to antiproliferative stimuli. p27(Kip1) is regulated by several posttranscriptional mechanisms, including subcellular localization. We have identi...

journal_title：Molecular and cellular biology

authors： Smitherman M,Lee K,Swanger J,Kapur R,Clurman BE

更新日期：2000-08-01 00:00:00

abstract：:CCAAT/enhancer-binding protein alpha (C/EBPalpha) is one of the key transcription factors that mediate lineage specification and differentiation of multipotent myeloid progenitors into mature granulocytes. Although C/EBPalpha is known to induce granulopoiesis while suppressing monocyte differentiation, it is unclear h...

journal_title：Molecular and cellular biology

authors： Ross SE,Radomska HS,Wu B,Zhang P,Winnay JN,Bajnok L,Wright WS,Schaufele F,Tenen DG,MacDougald OA

更新日期：2004-01-01 00:00:00

abstract：:Previous analyses have identified related cytoplasmic Lsm1-7p and nuclear Lsm2-8p complexes. Here we report that mature heat shock and MET mRNAs that are trapped in the nucleus due to a block in mRNA export were strongly stabilized in strains lacking Lsm6p or the nucleus-specific Lsm8p protein but not by the absence o...

journal_title：Molecular and cellular biology

authors： Kufel J,Bousquet-Antonelli C,Beggs JD,Tollervey D

更新日期：2004-11-01 00:00:00

abstract：:Nutritional excess and/or obesity represent well-known predisposition factors for the development of non-insulin-dependent diabetes mellitus (NIDDM). However, molecular links between obesity and NIDDM are only beginning to emerge. Here, we demonstrate that nutrients suppress phosphatidylinositol 3 (PI3)-kinase/Akt sig...

journal_title：Molecular and cellular biology

authors： Tzatsos A,Kandror KV

更新日期：2006-01-01 00:00:00

abstract：:The RAD10 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of UV-damaged DNA. We show that the RAD10 gene is also required for mitotic recombination. The rad10 delta mutation lowered the rate of intrachromosomal recombination of a his3 duplication in which one his3 allele has a del...

journal_title：Molecular and cellular biology

authors： Schiestl RH,Prakash S

更新日期：1990-06-01 00:00:00

abstract：:Smad proteins play a key role in the intracellular signaling of the transforming growth factor beta (TGF-beta) superfamily of extracellular polypeptides that initiate signaling from the cell surface through serine/threonine kinase receptors. A subclass of Smad proteins, including Smad6 and Smad7, has been shown to fun...

journal_title：Molecular and cellular biology

authors： Datta PK,Moses HL

更新日期：2000-05-01 00:00:00

abstract：:In vitro studies have implicated nuclear respiratory factor 1 (NRF-1) in the transcriptional expression of nuclear genes required for mitochondrial respiratory function, as well as for other fundamental cellular activities. We investigated here the in vivo function of NRF-1 in mammals by disrupting the gene in mice. A...

journal_title：Molecular and cellular biology

authors： Huo L,Scarpulla RC

更新日期：2001-01-01 00:00:00

abstract：:The functional receptor for insect ecdysteroid hormones is a heterodimer consisting of two nuclear hormone receptors, ecdysteroid receptor (EcR) and the retinoid X receptor homologue Ultraspiracle (USP). Although ecdysone is commonly thought to be a hormone precursor and 20-hydroxyecdysone (20E), the physiologically a...

journal_title：Molecular and cellular biology

authors： Wang SF,Ayer S,Segraves WA,Williams DR,Raikhel AS

更新日期：2000-06-01 00:00:00

abstract：:Microinjection of p21Ha-ras proteins into Xenopus laevis oocytes induces a rapid increase of 1,2-diacylglycerol (DAG) levels. The observed alterations in DAG levels were consistent with the ability of the protein to induce maturation, measured by germinal vesicle breakdown (GVBD). Both the increase in DAG levels and G...

journal_title：Molecular and cellular biology

authors： Lacal JC

更新日期：1990-01-01 00:00:00

abstract：:The retinoblastoma tumor suppressor protein (Rb) plays a vital role in regulating mammalian cell cycle progression and inactivation of Rb is necessary for entry into S phase. Rb is inactivated by phosphorylation upon growth factor stimulation of quiescent cells, facilitating the transition from G(1) phase to S phase. ...

journal_title：Molecular and cellular biology

authors： Dasgupta P,Sun J,Wang S,Fusaro G,Betts V,Padmanabhan J,Sebti SM,Chellappan SP

更新日期：2004-11-01 00:00:00

abstract：:Using nuclear runoff transcription assays we demonstrated that alpha interferon-mediated induction of transcription of four mRNAs in HeLa monolayer cells needed ongoing protein synthesis and that such a need could be obviated by pretreating the cells with gamma interferon which, by itself, did not induce transcription...

journal_title：Molecular and cellular biology

authors： Kusari J,Sen GC

更新日期：1987-01-01 00:00:00

abstract：:NIH3T3 cells transformed by mouse Int-2/Fgf-3 cDNA express a series of Int-2-related products representing discrete stages of processing and glycosylation. We confirm that in at least two highly transformed clonal lines, Int-2 products acquire further modifications and are efficiently secreted into the culture medium....

journal_title：Molecular and cellular biology

authors： Kiefer P,Peters G,Dickson C

更新日期：1991-12-01 00:00:00

abstract：:A characteristic feature of gene expression in eukaryotes is the addition of a 5'-terminal 7-methylguanine cap (m7GpppN) to nascent pre-mRNAs in the nucleus catalyzed by capping enzyme and cap methyltransferase. Small interfering RNA (siRNA) knockdown of cap methyltransferase in HeLa cells resulted in apoptosis as mea...

journal_title：Molecular and cellular biology

authors： Shafer B,Chu C,Shatkin AJ

更新日期：2005-04-01 00:00:00

abstract：:We sought to study the binding constraints placed on the nine-zinc-finger protein transcription factor IIIA (TFIIIA) by a histone octamer. To this end, five overlapping fragments of the Xenopus laevis oocyte and somatic 5S rRNA genes were reconstituted into nucleosomes, and it was subsequently shown that nucleosome tr...

journal_title：Molecular and cellular biology

authors： Howe L,Ausió J

更新日期：1998-03-01 00:00:00

abstract：:Activins and other members of the transforming growth factor beta family play a critical role in morphological changes of the epidermis that require epithelial cell movement. We investigated the molecular pathways in the transmission of activin signals that lead to actin reorganization and epithelial cell migration. W...

journal_title：Molecular and cellular biology

authors： Zhang L,Deng M,Parthasarathy R,Wang L,Mongan M,Molkentin JD,Zheng Y,Xia Y

更新日期：2005-01-01 00:00:00

abstract：:The transcription factor LSF, identified as a HeLa protein that binds the simian virus 40 late promoter, recognizes direct repeats with a center-to-center spacing of 10 bp. The characterization of two human cDNAs, representing alternatively spliced mRNAs, provides insight into the unusual DNA-binding and oligomerizati...

journal_title：Molecular and cellular biology

authors： Shirra MK,Zhu Q,Huang HC,Pallas D,Hansen U

更新日期：1994-08-01 00:00:00